FastQCFastQC Report
Fri 27 May 2016
SRR2140096_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140096_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6515863
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75400.042.0618441
CTTATAC73900.038.0477371
TATACAC82650.036.070883
GTATCAA127850.034.638021
TTATACA101700.028.5417442
ATCAACG155350.027.9208453
CAACGCA157150.027.495795
TCAACGC158300.027.3482864
TATCAAC163350.027.009072
AACGCAG165500.026.3833686
TACACAT117450.025.7352685
ATACACA129450.024.2120464
ACGCAGA183250.023.6695047
TACATGG205000.023.3772752
GTACATG210100.023.3018261
ACACATC124300.022.886626
ACATGGG204750.022.779783
ACATCTC125950.022.6192768
CGCAGAG194150.022.3406478
GTACTAG18450.021.0666661
Produced by FastQC (version 0.11.5)