##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140096_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6515863
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73076336933419	34.0	31.0	34.0	31.0	34.0
2	32.93965158567637	34.0	31.0	34.0	31.0	34.0
3	33.04933498448325	34.0	33.0	34.0	31.0	34.0
4	36.434547503531	37.0	37.0	37.0	35.0	37.0
5	36.2955722058613	37.0	37.0	37.0	35.0	37.0
6	36.320789893832945	37.0	37.0	37.0	35.0	37.0
7	36.32468592418226	37.0	37.0	37.0	35.0	37.0
8	36.334708541293764	37.0	37.0	37.0	35.0	37.0
9	38.23681790117441	39.0	39.0	39.0	37.0	39.0
10-11	38.14322860993241	39.0	38.5	39.0	37.0	39.0
12-13	38.12979454908736	39.0	38.5	39.0	37.0	39.0
14-15	39.62305683836508	41.0	39.0	41.0	37.0	41.0
16-17	39.59475859145596	41.0	39.0	41.0	37.0	41.0
18-19	39.63581393592837	41.0	39.5	41.0	37.0	41.0
20-21	39.65360569770922	41.0	40.0	41.0	37.0	41.0
22-23	39.60374857938598	41.0	39.5	41.0	37.0	41.0
24-25	39.58295356743686	41.0	39.0	41.0	37.0	41.0
26-27	39.46445476163316	41.0	39.0	41.0	37.0	41.0
28-29	39.39319830380314	40.5	39.0	41.0	37.0	41.0
30-31	39.31970745346861	40.0	39.0	41.0	36.0	41.0
32-33	39.23212097789627	40.0	39.0	41.0	36.0	41.0
34-35	39.18357741922726	40.0	39.0	41.0	36.0	41.0
36-37	39.13244765507551	40.0	39.0	41.0	35.0	41.0
38-39	39.05624193440697	40.0	39.0	41.0	35.0	41.0
40-41	39.00830304674448	40.0	39.0	41.0	35.0	41.0
42-43	38.99933821326744	40.0	39.0	41.0	35.0	41.0
44-45	38.9285831079879	40.0	38.0	41.0	35.0	41.0
46-47	38.81039890828448	40.0	38.0	41.0	35.0	41.0
48-49	38.79657644471847	40.0	38.0	41.0	35.0	41.0
50-51	38.645953082904214	40.0	38.0	41.0	35.0	41.0
52-53	38.48512447117666	40.0	38.0	41.0	34.5	41.0
54-55	38.280242455403965	40.0	37.0	41.0	34.0	41.0
56-57	38.111650247324775	40.0	37.0	41.0	34.0	41.0
58-59	37.94481362341508	39.5	36.5	41.0	34.0	41.0
60-61	37.70320506032314	39.0	36.0	41.0	33.5	41.0
62-63	37.54344203405426	39.0	35.5	41.0	33.0	41.0
64-65	37.278485251086295	38.5	35.0	40.0	33.0	41.0
66-67	37.04083317159261	38.0	35.0	40.0	33.0	41.0
68-69	36.77798584737218	37.0	35.0	40.0	33.0	41.0
70-71	36.33537589609796	37.0	35.0	39.0	32.5	41.0
72-73	36.07835607845213	36.0	35.0	39.0	32.0	41.0
74-75	35.72437301999	36.0	35.0	39.0	32.0	40.0
76-77	33.9537597113642	34.5	32.0	36.5	29.0	39.0
78-79	34.96224150273859	35.0	34.0	37.0	31.5	39.0
80-81	34.90871955526755	35.0	34.0	37.0	32.0	39.0
82-83	34.66045280591162	35.0	34.0	36.0	31.5	38.0
84-85	34.43547486879572	35.0	34.0	36.0	31.0	37.0
86-87	34.26465914341132	35.0	34.0	35.5	31.0	37.0
88-89	34.113652682787105	35.0	34.0	35.0	31.0	36.5
90-91	34.06027271242348	35.0	34.0	35.0	31.0	36.0
92-93	33.94782029303218	35.0	34.0	35.0	31.0	36.0
94-95	33.80834742378484	35.0	34.0	35.0	31.0	36.0
96-97	33.8405198943447	35.0	34.0	35.0	31.0	35.5
98-99	33.875709694676544	35.0	34.0	35.0	31.0	35.0
100-101	32.6628079816125	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	6.0
20	25.0
21	72.0
22	357.0
23	1032.0
24	2644.0
25	5767.0
26	10962.0
27	17887.0
28	27632.0
29	39188.0
30	53537.0
31	71773.0
32	97233.0
33	138577.0
34	220653.0
35	373301.0
36	773887.0
37	1656250.0
38	2037797.0
39	966483.0
40	20799.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.47923874396991	19.979210735400667	12.818424819551916	24.723125701077507
2	15.273657656396686	20.64605549567535	40.56914383481228	23.511143013115685
3	18.481408889030085	26.2220509470092	30.049499457398284	25.247040706562434
4	12.423005210514708	17.490591806488258	38.143128546441204	31.943274436555836
5	13.072005964520738	38.02289888538172	35.65555322449229	13.249541925605248
6	30.544821011236024	37.524532568999675	18.29053805594623	13.640108363818074
7	26.881765316428535	32.04247848673307	22.849037802053235	18.22671839478516
8	25.351822160779008	35.78720117350534	21.04556219183859	17.81541447387706
9	25.88808321358703	15.954688399488079	20.631063379364104	37.526165007560785
10-11	24.81680796336313	26.170355625675278	28.545943424025143	20.46689298693645
12-13	25.51818369891446	23.497176724521612	28.510923233795328	22.4737163427686
14-15	22.666560055053335	24.905948759205035	25.724842588004076	26.702648597737554
16-17	22.529525252449293	28.17607583216529	26.35073819078148	22.94366072460394
18-19	22.483974877924844	27.214200175786385	27.987451547093606	22.314373399195166
20-21	23.192432420818058	26.64937251465309	27.640173181965377	22.518021882563474
22-23	23.081220721338127	26.779735133104953	27.457235752042493	22.681808393514423
24-25	22.90829873843432	26.90658279664739	27.708199938994877	22.476918525923416
26-27	22.83031268090517	27.222793782313403	27.675753448426764	22.27114008835466
28-29	22.47390815122217	27.042399840075404	27.814472407859352	22.66921960084308
30-31	22.869248807683164	27.310801232298033	27.372277604145424	22.447672355873387
32-33	23.173064795965704	26.95462403693073	27.47249591956105	22.399815247542513
34-35	22.752394233928424	27.004264257698974	27.685369473839888	22.557972034532717
36-37	22.634534850691303	27.23248434407754	27.524919832595362	22.608060972635794
38-39	22.566625075078473	26.835087467989556	27.836529037462586	22.761758419469384
40-41	22.701271860738117	26.714989030243743	28.005184881434918	22.578554227583222
42-43	22.90836055637019	26.688621137346143	27.723927399011366	22.6790909072723
44-45	22.626568112225442	27.333636406059973	27.421120955538004	22.61867452617658
46-47	23.021435990699622	26.811770490742816	27.045246206623514	23.121547311934055
48-49	22.94477421089586	26.701036644483583	27.13923604091607	23.214953103704485
50-51	22.51743184513376	26.92028736335899	27.425538303093532	23.136742488413713
52-53	22.7201070834066	27.10996379966355	27.352695457484316	22.817233659445534
54-55	22.795745838949617	27.02670315376225	27.026968878107528	23.150582129180606
56-57	23.117783932171672	27.205032446539366	26.781278993371828	22.89590462791713
58-59	22.64076016039717	27.485589412822925	27.31164495739148	22.56200546938842
60-61	23.010786559679055	27.836683002537953	26.504151176023182	22.648379261759807
62-63	22.760673584557566	27.9269203435467	26.52500753051099	22.787398541384743
64-65	22.68064850122709	27.731126158733225	26.64285441393539	22.945370926104296
66-67	22.84568357604161	27.56661306481832	26.51877378705984	23.06892957208023
68-69	22.840761322539795	27.3768069870212	26.77911808474591	23.003313605693094
70-71	22.904697810942835	27.0149093555259	26.821201364487813	23.25919146904345
72-73	22.94505031204229	27.176126974925307	26.837623016719142	23.04119969631326
74-75	22.739262892324465	27.370498239448704	26.876622173239035	23.0136166949878
76-77	22.887738380131754	27.318489652865523	26.702048781262498	23.09172318574022
78-79	22.95389072165363	27.261005132411388	26.673652616363807	23.111451529571173
80-81	22.830076624839812	27.345094971202016	26.687794015479803	23.137034388478362
82-83	23.073469689504254	27.155946270883263	26.918807049396243	22.851776990216237
84-85	23.210877418515473	27.1379184195703	26.648363888048156	23.00284027386607
86-87	22.82630077051193	27.309377504873495	26.9057800523646	22.95854167224997
88-89	23.059693913809692	27.06600449117642	26.815928110259907	23.058373484753982
90-91	23.073799578870688	27.14199324955767	26.82701762809132	22.957189543480318
92-93	23.147565001148457	27.12409999324307	26.79606275693052	22.932272248677947
94-95	22.864593957551353	27.26263830766148	26.87453889957738	22.998228835209787
96-97	22.817047739803648	27.22610286110948	26.939156223695367	23.0176931753915
98-99	23.196561730365776	27.778332041107966	26.929786554775152	22.095319673751103
100-101	27.647448733423047	34.85436638780855	27.27876716657649	10.21941771219191
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	848.0
1	901.0
2	1647.5
3	2500.0
4	3533.5
5	5797.0
6	7533.5
7	8501.5
8	8956.5
9	8462.0
10	7381.0
11	6427.0
12	7001.5
13	9592.5
14	15366.0
15	22649.0
16	27996.5
17	30632.0
18	30691.5
19	28723.0
20	25977.0
21	23671.0
22	23056.0
23	25417.5
24	30681.5
25	38175.0
26	48672.0
27	61025.0
28	72004.0
29	83744.5
30	95330.0
31	105449.0
32	118705.0
33	134204.5
34	147994.0
35	161899.5
36	178670.0
37	193429.0
38	201336.5
39	206500.5
40	214626.0
41	222443.0
42	227983.5
43	236586.5
44	250921.0
45	269039.5
46	285968.0
47	301456.0
48	308977.0
49	308538.0
50	308138.5
51	306007.5
52	296505.5
53	285049.0
54	273642.0
55	255789.5
56	236953.5
57	218348.0
58	197403.5
59	172740.0
60	143167.5
61	113311.5
62	88621.0
63	68729.0
64	52136.5
65	38772.5
66	28755.5
67	21725.5
68	16526.0
69	12281.5
70	9338.0
71	7113.5
72	5231.0
73	3888.0
74	2851.0
75	1962.5
76	1282.5
77	828.0
78	541.5
79	377.5
80	285.5
81	209.0
82	142.5
83	98.5
84	67.0
85	44.0
86	27.5
87	20.0
88	15.5
89	9.5
90	5.0
91	2.5
92	2.0
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001166384253321471
3	5.218034817490792E-4
4	0.0
5	0.0
6	5.371506429769932E-4
7	0.0
8	0.0
9	6.138864491165637E-5
10-11	3.5298470824202414E-4
12-13	4.143733531536805E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	8.445433020767857E-5
28-29	7.682772190054881E-6
30-31	5.387260529535387E-5
32-33	2.933060157372571E-4
34-35	1.54942755237007E-5
36-37	8.17809491824086E-4
38-39	1.4136093829736406E-4
40-41	6.341582860667419E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	1.6773483002003926E-5
52-53	0.0
54-55	5.485891871871167E-4
56-57	6.0753146340356316E-5
58-59	0.0
60-61	8.913475026091969E-4
62-63	0.0
64-65	0.0
66-67	2.789179582350109E-5
68-69	0.0
70-71	0.0
72-73	5.8125538569443305E-5
74-75	1.961070399289622E-5
76-77	0.0
78-79	3.0155169455466994E-5
80-81	0.0
82-83	0.0
84-85	5.229163378830192E-5
86-87	5.402314605803696E-4
88-89	7.514631255434019E-5
90-91	2.1784048195028226E-5
92-93	2.2117598608272223E-5
94-95	1.1232652151608903E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	92.0
20-21	409.0
22-23	858.0
24-25	1553.0
26-27	3202.0
28-29	8576.0
30-31	18144.0
32-33	22374.0
34-35	30025.0
36-37	48687.0
38-39	60157.0
40-41	58174.0
42-43	63232.0
44-45	74790.0
46-47	74953.0
48-49	72112.0
50-51	65484.0
52-53	63015.0
54-55	70177.0
56-57	74923.0
58-59	76133.0
60-61	76184.0
62-63	76285.0
64-65	76846.0
66-67	82722.0
68-69	71925.0
70-71	67992.0
72-73	62465.0
74-75	61628.0
76-77	62251.0
78-79	65592.0
80-81	66643.0
82-83	62216.0
84-85	60522.0
86-87	61623.0
88-89	66235.0
90-91	69144.0
92-93	69584.0
94-95	73271.0
96-97	112872.0
98-99	505377.0
100-101	3777416.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.66758970651907
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.9685261808102	71.84177503221588
2	7.829408304607501	12.788178101309969
3	2.2033786646479205	5.398338742576928
4	0.8986350078407207	2.935574204650021
5	0.41659098519645776	1.7010990827229435
6	0.23715579599244008	1.162076534618012
7	0.13401509784654356	0.7661283017787369
8	0.08998297493285162	0.5878954141910493
9	0.05519737888071031	0.4057053202174617
>10	0.16365902893353085	1.9358868038520394
>50	0.0020217432717715713	0.10624413015053712
>100	0.0011268564153456158	0.18862672112958373
>500	2.3487382795808368E-4	0.10922539235724063
>1k	6.710679618995976E-5	0.07324621822957046
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5347161227914094E-5
2	0.0	0.0	0.0	0.0	1.5347161227914094E-5
3	0.0	1.5347161227914094E-5	0.0	0.0	1.5347161227914094E-5
4	0.0	3.069432245582819E-5	0.0	0.0	1.5347161227914094E-5
5	0.0	3.069432245582819E-5	0.0	0.0	1.5347161227914094E-5
6	2.455545796466255E-4	3.069432245582819E-5	0.0	0.0	4.604148368374228E-5
7	2.455545796466255E-4	3.069432245582819E-5	0.0	0.0	4.604148368374228E-5
8	2.455545796466255E-4	3.069432245582819E-5	0.0	0.0	4.604148368374228E-5
9	2.455545796466255E-4	3.069432245582819E-5	0.0	0.0	4.604148368374228E-5
10-11	2.5322816026058256E-4	3.069432245582819E-5	0.0	0.0	4.604148368374228E-5
12-13	2.609017408745396E-4	3.836790306978523E-5	0.0	0.0	4.604148368374228E-5
14-15	2.9159606333036773E-4	6.138864491165637E-5	0.0	2.3020741841871142E-5	6.138864491165637E-5
16-17	4.604148368374228E-4	7.673580613957046E-5	0.0	3.069432245582819E-5	7.673580613957046E-5
18-19	4.604148368374228E-4	7.673580613957046E-5	0.0	3.069432245582819E-5	7.673580613957046E-5
20-21	4.604148368374228E-4	9.208296736748456E-5	0.0	4.604148368374228E-5	7.673580613957046E-5
22-23	4.7576199806533687E-4	9.208296736748456E-5	0.0	6.138864491165637E-5	7.673580613957046E-5
24-25	4.91109159293251E-4	9.208296736748456E-5	0.0	9.208296736748456E-5	7.673580613957046E-5
26-27	4.91109159293251E-4	9.208296736748456E-5	0.0	1.5347161227914093E-4	9.208296736748456E-5
28-29	4.91109159293251E-4	9.208296736748456E-5	0.0	7.750316420096616E-4	9.975654798144161E-5
30-31	4.91109159293251E-4	9.975654798144161E-5	0.0	0.0032305774384759163	1.0743012859539865E-4
32-33	4.91109159293251E-4	1.0743012859539865E-4	0.0	0.007213165777119623	1.2277728982331275E-4
34-35	5.06456320521165E-4	1.0743012859539865E-4	0.0	0.01202450082207069	1.3812445105122683E-4
36-37	5.06456320521165E-4	1.304508704372698E-4	0.0	0.01851635002147835	1.3812445105122683E-4
38-39	5.06456320521165E-4	1.6114519289309797E-4	0.0	0.029105891268739074	1.3812445105122683E-4
40-41	5.06456320521165E-4	1.7649235412101207E-4	0.0	0.04053185280292112	1.3812445105122683E-4
42-43	5.985392878886496E-4	2.148602571907973E-4	0.0	0.05005476634484181	1.4579803166518386E-4
44-45	6.445807715723919E-4	2.148602571907973E-4	0.0	0.06088986217174916	1.6114519289309797E-4
46-47	6.67601513414263E-4	2.148602571907973E-4	0.0	0.07205492196505667	1.7649235412101207E-4
48-49	6.7527509402822E-4	2.2253383780475436E-4	0.0	0.08283630272766632	2.302074184187114E-4
50-51	6.906222552561342E-4	2.3788099903266843E-4	0.0	0.09372511361887137	2.302074184187114E-4
52-53	7.059694164840483E-4	2.455545796466255E-4	0.0	0.10359333828842013	2.839224827164107E-4
54-55	7.366637389398764E-4	2.609017408745396E-4	0.0	0.11353829876410845	3.453111276280671E-4
56-57	7.673580613957046E-4	2.609017408745396E-4	0.0	0.12318398959585247	3.5298470824202414E-4
58-59	8.594410287631892E-4	2.609017408745396E-4	0.0	0.1327145767183871	3.5298470824202414E-4
60-61	8.747881899911033E-4	2.7624890210245365E-4	0.0	0.14185381122960994	3.683318694699382E-4
62-63	8.747881899911033E-4	2.7624890210245365E-4	0.0	0.15008756322838585	3.683318694699382E-4
64-65	8.747881899911033E-4	2.7624890210245365E-4	0.0	0.15732374974734736	3.683318694699382E-4
66-67	9.208296736748456E-4	3.0694322455828186E-4	0.0	0.16498965678069044	3.683318694699382E-4
68-69	9.208296736748456E-4	3.0694322455828186E-4	0.0	0.17291646555490808	3.683318694699382E-4
70-71	9.208296736748456E-4	3.1461680517223887E-4	0.0	0.18056702542702324	3.913526113118093E-4
72-73	9.438504155167167E-4	3.2229038578619594E-4	0.0	0.18620710717828168	4.143733531536805E-4
74-75	9.822183185865018E-4	3.2229038578619594E-4	0.0	0.1904199029353441	4.143733531536805E-4
76-77	9.97565479814416E-4	3.3763754701411E-4	0.0	0.19471710807916004	4.143733531536805E-4
78-79	9.97565479814416E-4	3.3763754701411E-4	0.0	0.19835438529017568	4.143733531536805E-4
80-81	9.97565479814416E-4	3.3763754701411E-4	0.0	0.1999965315415625	4.297205143815946E-4
82-83	9.97565479814416E-4	3.3763754701411E-4	0.0	0.20068715379681862	4.527412562234657E-4
84-85	0.0010743012859539863	3.3763754701411E-4	0.0	0.20113989505304208	4.604148368374228E-4
86-87	0.0010743012859539863	3.3763754701411E-4	0.0	0.20129336666532122	4.7576199806533687E-4
88-89	0.0010819748665679436	3.3763754701411E-4	0.0	0.20141614395514454	4.7576199806533687E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7540	0.0	42.061844	1
CTTATAC	7390	0.0	38.047737	1
TATACAC	8265	0.0	36.07088	3
GTATCAA	12785	0.0	34.63802	1
TTATACA	10170	0.0	28.541744	2
ATCAACG	15535	0.0	27.920845	3
CAACGCA	15715	0.0	27.49579	5
TCAACGC	15830	0.0	27.348286	4
TATCAAC	16335	0.0	27.00907	2
AACGCAG	16550	0.0	26.383368	6
TACACAT	11745	0.0	25.735268	5
ATACACA	12945	0.0	24.212046	4
ACGCAGA	18325	0.0	23.669504	7
TACATGG	20500	0.0	23.377275	2
GTACATG	21010	0.0	23.301826	1
ACACATC	12430	0.0	22.88662	6
ACATGGG	20475	0.0	22.77978	3
ACATCTC	12595	0.0	22.619276	8
CGCAGAG	19415	0.0	22.340647	8
GTACTAG	1845	0.0	21.066666	1
>>END_MODULE