FastQCFastQC Report
Fri 27 May 2016
SRR2140095_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140095_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6698930
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78000.036.3791241
GTATCAA124900.031.1562061
ATCAACG138700.027.2283133
CAACGCA140800.026.7063125
TCAACGC145700.026.005174
AACGCAG147500.025.66046
TATCAAC153600.025.1211322
ACGCAGA164500.022.8591677
CGCAGAG166500.022.6092138
TACATGG181250.022.035442
GTACATG185450.021.845951
ACATGGG178500.021.7315373
CATGGGG125150.019.757584
GCAGAGT203700.018.5205469
TAACGCC5350.018.395214
GTACTAG22900.017.7272151
AGAGTAC181800.016.66873710-11
CAGAGTA196050.016.57618510-11
GAGTACT117750.015.96696812-13
ATGGGGG88650.015.9583365
Produced by FastQC (version 0.11.5)