##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140095_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6698930
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.80251861118119	34.0	31.0	34.0	31.0	34.0
2	33.002340373761186	34.0	33.0	34.0	31.0	34.0
3	33.112238670951925	34.0	33.0	34.0	31.0	34.0
4	36.47355965803494	37.0	37.0	37.0	35.0	37.0
5	36.3411653801428	37.0	37.0	37.0	35.0	37.0
6	36.36848436989191	37.0	37.0	37.0	35.0	37.0
7	36.37596153415546	37.0	37.0	37.0	35.0	37.0
8	36.38746949139639	37.0	37.0	37.0	35.0	37.0
9	38.29928510971155	39.0	39.0	39.0	37.0	39.0
10-11	38.21138062347271	39.0	39.0	39.0	37.0	39.0
12-13	38.19317308883657	39.0	39.0	39.0	37.0	39.0
14-15	39.71894078606583	41.0	40.0	41.0	38.0	41.0
16-17	39.700857674285295	41.0	40.0	41.0	38.0	41.0
18-19	39.73794650787514	41.0	40.0	41.0	38.0	41.0
20-21	39.757718128492684	41.0	40.0	41.0	38.0	41.0
22-23	39.71190653064213	41.0	40.0	41.0	38.0	41.0
24-25	39.69593991216239	41.0	40.0	41.0	37.0	41.0
26-27	39.58879860152861	41.0	40.0	41.0	37.0	41.0
28-29	39.52838890093969	41.0	39.0	41.0	37.0	41.0
30-31	39.47048234665506	40.5	39.0	41.0	37.0	41.0
32-33	39.39370717578569	40.0	39.0	41.0	36.5	41.0
34-35	39.36140249432987	40.0	39.0	41.0	36.0	41.0
36-37	39.328125431997975	40.0	39.0	41.0	36.0	41.0
38-39	39.27073696720653	40.0	39.0	41.0	36.0	41.0
40-41	39.21175236400873	40.0	39.0	41.0	36.0	41.0
42-43	39.208824922846034	40.0	39.0	41.0	35.0	41.0
44-45	39.15667121865181	40.0	39.0	41.0	35.0	41.0
46-47	39.05398382768946	40.0	39.0	41.0	35.0	41.0
48-49	39.05439574206504	40.0	39.0	41.0	35.0	41.0
50-51	38.95742574124486	40.0	38.5	41.0	35.0	41.0
52-53	38.80864691717329	40.0	38.0	41.0	35.0	41.0
54-55	38.623896332025204	40.0	38.0	41.0	35.0	41.0
56-57	38.49280086500101	40.0	38.0	41.0	35.0	41.0
58-59	38.35151237553219	40.0	37.0	41.0	34.0	41.0
60-61	38.14299363353848	40.0	37.0	41.0	34.0	41.0
62-63	38.00157130487793	39.5	36.0	41.0	34.0	41.0
64-65	37.75074530752041	39.0	36.0	41.0	34.0	41.0
66-67	37.52721685635191	39.0	35.0	40.5	34.0	41.0
68-69	37.279877076400716	38.5	35.0	40.0	33.5	41.0
70-71	36.840435037105664	37.5	35.0	40.0	33.0	41.0
72-73	36.5819652626663	37.0	35.0	39.5	33.0	41.0
74-75	36.21918036364319	36.5	35.0	39.0	32.5	41.0
76-77	34.40050956561977	34.5	33.0	37.0	30.0	39.0
78-79	35.39611491841585	35.0	34.5	37.5	32.0	39.0
80-81	35.30008362230636	35.0	35.0	37.0	32.0	39.0
82-83	35.00730196876087	35.0	34.5	37.0	32.0	39.0
84-85	34.74144080926953	35.0	34.0	36.0	32.0	37.5
86-87	34.53492125411252	35.0	34.0	36.0	32.0	37.0
88-89	34.34966570420991	35.0	34.0	36.0	32.0	37.0
90-91	34.26620958246964	35.0	34.0	35.0	32.0	36.0
92-93	34.133204965356185	35.0	34.0	35.0	32.0	36.0
94-95	33.97215987659646	35.0	34.0	35.0	31.0	36.0
96-97	33.99261433408408	35.0	34.0	35.0	31.5	36.0
98-99	34.01581485804367	35.0	34.0	35.0	32.0	35.5
100-101	32.801161978027764	34.5	32.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	15.0
20	30.0
21	70.0
22	247.0
23	760.0
24	2267.0
25	4816.0
26	9104.0
27	15311.0
28	23041.0
29	33369.0
30	45369.0
31	61743.0
32	83947.0
33	118972.0
34	190065.0
35	318442.0
36	663141.0
37	1519218.0
38	2292429.0
39	1282110.0
40	34460.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.29491874075412	20.29854021463129	13.020318170215242	24.386222874399344
2	14.728092223126033	21.29683882046284	41.16812317196655	22.80694578444458
3	18.221317263009247	26.439798235230015	30.310693264107936	25.028191237652802
4	12.072942992388338	17.739773366791415	39.844960314557696	30.342323326262555
5	12.731495925468694	38.224507495973235	36.25055046104378	12.79344611751429
6	29.5514239706794	38.56551865385841	19.071777210289017	12.811280165173164
7	26.13638297459445	32.58812974609378	23.937554206418042	17.33793307289373
8	24.304060499214053	36.73980770063279	21.719752258942844	17.23637954121031
9	25.480983602005637	16.377558263417928	21.685496293511992	36.45596184106444
10-11	24.104306638288882	26.760316335399715	29.361227454795767	19.774149571515633
12-13	24.822200459134635	24.292502067315016	29.366919809971968	21.518377663578374
14-15	21.779105021249663	25.7692721076351	26.950871258544275	25.500751612570966
16-17	21.584014163455954	28.713242264063066	27.681771566503905	22.020972005977075
18-19	21.401873134963346	28.276232174392028	29.10503617741938	21.216858513225244
20-21	22.35804562979789	27.585995325326046	28.770487899873544	21.28547114500252
22-23	22.049797450535	27.51596429587458	28.58509791029209	21.84914034329833
24-25	21.764517252565646	27.570940445974834	28.83942987074995	21.82511243070957
26-27	21.875978822701743	27.763694929203623	28.583587071063743	21.77673917703089
28-29	21.678922098027805	28.08380439999707	28.542020960068665	21.69525254190646
30-31	22.008476665505412	27.825752372211227	28.529215559465015	21.636555402818345
32-33	21.765469451449256	27.956308816803087	28.456033225476695	21.82218850627096
34-35	21.784852112814274	28.067728722769843	28.534753601876915	21.61266556253897
36-37	21.858897932386835	27.89219020710166	28.511867926067747	21.737043934443765
38-39	21.74217088473398	27.99393333511304	28.563977677520754	21.699918102632225
40-41	21.94238206442689	27.8765936925286	28.474722664160794	21.70630157888371
42-43	21.879846607754697	27.985682805227146	28.299322105845288	21.835148481172865
44-45	21.971484395577335	28.232563430948375	28.036583903006516	21.759368270467775
46-47	21.927106814155557	28.031715071093476	27.896111626111242	22.145066488639724
48-49	21.981748854269316	27.995832194722365	27.995579472001907	22.026839479006405
50-51	21.836911463433932	28.13818860291634	27.896902877705564	22.127997055944164
52-53	21.952824007053763	28.155207801722543	27.722938501968393	22.169029689255307
54-55	21.977666520217802	28.07909861429173	27.76265581040513	22.18057905508534
56-57	22.086442449495088	28.09713748108432	27.67492374901972	22.141496320400865
58-59	21.992818640338943	28.1545572933366	27.848618130732078	22.004005935592374
60-61	22.067367088976503	28.143872685900046	27.723087338131293	22.065672886992164
62-63	22.08624092118713	28.01682224080455	27.658194797110568	22.23874204089775
64-65	21.91440347484284	28.13012283140689	27.71494596229875	22.24052773145152
66-67	22.009519825098216	28.18875440461253	27.525214700217404	22.276511070071848
68-69	22.0176144982121	28.150640333142164	27.644720686999435	22.187024481646297
70-71	21.997025529621393	27.839249291052077	27.649259493305568	22.514465686020962
72-73	22.034029828291285	27.95654815184041	27.80200613039164	22.207415889476668
74-75	22.01548069473774	28.044201801693625	27.648188280716905	22.292129222851727
76-77	21.955155298033585	28.02457508387176	27.543534830301663	22.47673478779299
78-79	22.079876739027085	28.01119839220006	27.492693188422106	22.416231680350744
80-81	21.96733443418559	28.05633829919529	27.51489217380637	22.46143509281275
82-83	22.145141646486643	27.892249854114183	27.763697509063217	22.198910990335953
84-85	22.38389875922338	27.923636588482626	27.530967888206437	22.161496764087556
86-87	21.910116056819675	28.036352122694158	27.690664737870623	22.362867082615544
88-89	22.18062335854835	27.885945889362183	27.63306115131848	22.300369600770985
90-91	22.212084316589475	27.945358564922874	27.623718181759514	22.21883893672814
92-93	22.237528507151332	27.86693898182962	27.64250953188665	22.2530229791324
94-95	21.974879659181656	27.98433681749501	27.81008802045905	22.230695502864283
96-97	21.95134027778877	27.980075066679838	27.855227706256454	22.21335694927494
98-99	22.25795776092197	28.603457730340143	27.800934265979237	21.337650242758652
100-101	26.319332107708775	35.57964293766993	28.432271742044797	9.6687532125765
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	670.0
1	722.5
2	1396.0
3	2146.5
4	3218.5
5	5583.0
6	7294.0
7	8113.0
8	8303.0
9	7585.5
10	6481.0
11	5563.5
12	6238.5
13	8925.5
14	14535.5
15	21676.5
16	27153.5
17	30204.0
18	31018.0
19	30187.0
20	28757.5
21	27747.0
22	28671.5
23	32804.0
24	40073.5
25	50212.5
26	64162.0
27	79495.5
28	95025.0
29	112233.0
30	127358.5
31	141813.5
32	158134.5
33	173850.0
34	190848.5
35	208992.5
36	226418.5
37	242895.0
38	252552.0
39	257744.0
40	266400.5
41	273935.0
42	278334.0
43	283744.0
44	290037.0
45	296785.5
46	300976.0
47	304447.0
48	304559.0
49	296796.5
50	288499.5
51	279952.5
52	267558.5
53	254311.0
54	239247.5
55	218969.0
56	198152.0
57	175531.0
58	151260.0
59	130349.5
60	109710.5
61	88714.5
62	69492.0
63	53287.5
64	39951.0
65	28991.5
66	21105.5
67	15844.5
68	12072.5
69	8856.0
70	6400.5
71	4754.5
72	3506.0
73	2519.0
74	1737.0
75	1198.5
76	844.0
77	540.0
78	337.5
79	231.0
80	158.5
81	104.0
82	77.5
83	60.0
84	41.5
85	27.0
86	18.5
87	14.5
88	10.0
89	5.5
90	2.5
91	1.5
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001985391696882935
3	5.971102847768227E-4
4	0.0
5	0.0
6	3.1348289950783184E-4
7	0.0
8	0.0
9	1.4927757119420565E-5
10-11	4.1051332078406553E-4
12-13	3.508022923063833E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.9746891257222463E-5
28-29	1.4947772482944591E-5
30-31	5.990996131913348E-5
32-33	2.4800903233864927E-4
34-35	3.774363991359726E-5
36-37	6.913115433454237E-4
38-39	2.3013444914788116E-4
40-41	6.20264282205362E-5
42-43	0.0
44-45	7.933429639880208E-6
46-47	1.689108211993698E-4
48-49	5.7066388182692335E-5
50-51	4.377083605352562E-4
52-53	0.0
54-55	6.67721215404751E-4
56-57	7.701032896758926E-5
58-59	8.66560362818425E-6
60-61	9.2170760994282E-4
62-63	0.0
64-65	0.0
66-67	5.470008173103878E-5
68-69	9.24121578543819E-6
70-71	1.874035925643502E-5
72-73	7.598397346031775E-5
74-75	2.886465220258945E-5
76-77	4.8748456501996E-5
78-79	1.1858083640795325E-4
80-81	1.002232472833236E-5
82-83	1.0166257966152665E-5
84-85	2.061090304404488E-5
86-87	5.954328420699E-4
88-89	7.416590637783355E-5
90-91	0.0
92-93	2.1853975451210835E-5
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	184.0
20-21	551.0
22-23	978.0
24-25	1670.0
26-27	3744.0
28-29	9811.0
30-31	22741.0
32-33	27436.0
34-35	36779.0
36-37	58736.0
38-39	70602.0
40-41	68849.0
42-43	73790.0
44-45	84991.0
46-47	84296.0
48-49	80793.0
50-51	73226.0
52-53	66524.0
54-55	71560.0
56-57	73543.0
58-59	73128.0
60-61	73592.0
62-63	68654.0
64-65	69774.0
66-67	74195.0
68-69	74115.0
70-71	73423.0
72-73	67909.0
74-75	68161.0
76-77	67811.0
78-79	70581.0
80-81	71526.0
82-83	67222.0
84-85	65080.0
86-87	66716.0
88-89	69534.0
90-91	72810.0
92-93	72522.0
94-95	76114.0
96-97	114684.0
98-99	504930.0
100-101	3825645.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.56704651405465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.92614917673929	74.31295644568335
2	7.3697327369574	12.31733596850941
3	2.022113223726573	5.069460894715306
4	0.7903010714157817	2.6417250558043945
5	0.3688888010404717	1.5413473797531476
6	0.18936576394472507	0.9494842562243
7	0.10371756746208409	0.6067159549100009
8	0.0684955179758379	0.45791745093528907
9	0.04263172532549044	0.32063466359246956
>10	0.11603520774497436	1.3962249163996443
>50	0.001614273318791465	0.0890584684259806
>100	6.613074413999034E-4	0.12395268235304376
>500	2.349015890777721E-4	0.10919204464106237
>1k	5.872531804240381E-5	0.06399381805260013
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.4927757119420565E-5	0.0
4	0.0	0.0	0.0	4.4783271358261695E-5	0.0
5	0.0	0.0	0.0	4.4783271358261695E-5	0.0
6	0.0	0.0	0.0	4.4783271358261695E-5	0.0
7	0.0	0.0	0.0	5.971102847768226E-5	0.0
8	0.0	0.0	0.0	5.971102847768226E-5	0.0
9	0.0	0.0	0.0	8.956654271652339E-5	0.0
10-11	0.0	0.0	0.0	1.0449429983594395E-4	0.0
12-13	0.0	0.0	0.0	1.1942205695536452E-4	7.4638785597102825E-6
14-15	0.0	0.0	0.0	1.1942205695536452E-4	2.985551423884113E-5
16-17	0.0	0.0	0.0	1.2688593551507478E-4	4.4783271358261695E-5
18-19	0.0	0.0	0.0	1.3434981407478506E-4	7.463878559710282E-5
20-21	0.0	0.0	0.0	1.5674144975391592E-4	8.956654271652339E-5
22-23	0.0	0.0	0.0	3.2841065662725236E-4	1.1942205695536452E-4
24-25	0.0	0.0	0.0	5.224714991797197E-4	1.4927757119420564E-4
26-27	0.0	0.0	0.0	6.86676827493346E-4	1.716692068733365E-4
28-29	0.0	1.4927757119420565E-5	0.0	0.0017540114615319162	1.8659696399275707E-4
30-31	0.0	1.4927757119420565E-5	0.0	0.004679851856938347	1.9406084255246732E-4
32-33	0.0	1.4927757119420565E-5	0.0	0.009859783577377282	1.9406084255246732E-4
34-35	0.0	1.4927757119420565E-5	0.0	0.016181688717451893	2.089885996718879E-4
36-37	0.0	1.4927757119420565E-5	0.0	0.02565335060972424	2.089885996718879E-4
38-39	0.0	1.4927757119420565E-5	0.0	0.0427605602685802	2.089885996718879E-4
40-41	0.0	1.4927757119420565E-5	0.0	0.08083380480166236	2.089885996718879E-4
42-43	0.0	1.4927757119420565E-5	0.0	0.0961795391204267	2.2391635679130847E-4
44-45	0.0	1.4927757119420565E-5	0.0	0.11595135342509924	2.3884411391072904E-4
46-47	0.0	1.4927757119420565E-5	0.0	0.1379921868119237	2.686996281495702E-4
48-49	0.0	1.4927757119420565E-5	0.0	0.15708478816766258	3.284106566272524E-4
50-51	0.0	1.4927757119420565E-5	0.0	0.17699095228640993	3.284106566272524E-4
52-53	0.0	1.4927757119420565E-5	0.0	0.20312199112395563	3.5080229230638325E-4
54-55	0.0	1.4927757119420565E-5	0.0	0.25223431204684926	3.8065780654522434E-4
56-57	0.0	1.4927757119420565E-5	0.0	0.30414558742963427	4.030494422243552E-4
58-59	0.0	1.4927757119420565E-5	0.0	0.3555567829489187	4.179771993437758E-4
60-61	0.0	1.4927757119420565E-5	0.0	0.38818139613341235	4.179771993437758E-4
62-63	0.0	1.4927757119420565E-5	0.0	0.40468403162893174	4.776882278214581E-4
64-65	0.0	1.4927757119420565E-5	0.0	0.42006708534049464	4.8515210638116834E-4
66-67	0.0	1.4927757119420565E-5	0.0	0.4286430818056018	5.000798635005889E-4
68-69	0.0	1.4927757119420565E-5	0.0	0.43661450410737235	5.224714991797197E-4
70-71	0.0	1.4927757119420565E-5	0.0	0.4441754130883589	5.373992562991403E-4
72-73	0.0	1.4927757119420565E-5	0.0	0.44998977448637323	6.120380418962432E-4
74-75	0.0	1.4927757119420565E-5	0.0	0.454632306950513	6.195019204559534E-4
76-77	0.0	1.4927757119420565E-5	0.0	0.4592748394146528	6.34429677575374E-4
78-79	0.0	1.4927757119420565E-5	0.0	0.46320083953706037	6.418935561350842E-4
80-81	0.0	1.4927757119420565E-5	0.0	0.4650220259056297	6.717490703739254E-4
82-83	0.0	1.4927757119420565E-5	0.0	0.46571616661168275	7.165323417321871E-4
84-85	0.0	1.4927757119420565E-5	0.0	0.46623863811086247	7.165323417321871E-4
86-87	0.0	1.4927757119420565E-5	0.0	0.4664252350748552	7.239962202918973E-4
88-89	0.0	1.4927757119420565E-5	0.0	0.4664924099818926	7.314600988516076E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7800	0.0	36.379124	1
GTATCAA	12490	0.0	31.156206	1
ATCAACG	13870	0.0	27.228313	3
CAACGCA	14080	0.0	26.706312	5
TCAACGC	14570	0.0	26.00517	4
AACGCAG	14750	0.0	25.6604	6
TATCAAC	15360	0.0	25.121132	2
ACGCAGA	16450	0.0	22.859167	7
CGCAGAG	16650	0.0	22.609213	8
TACATGG	18125	0.0	22.03544	2
GTACATG	18545	0.0	21.84595	1
ACATGGG	17850	0.0	21.731537	3
CATGGGG	12515	0.0	19.75758	4
GCAGAGT	20370	0.0	18.520546	9
TAACGCC	535	0.0	18.39521	4
GTACTAG	2290	0.0	17.727215	1
AGAGTAC	18180	0.0	16.668737	10-11
CAGAGTA	19605	0.0	16.576185	10-11
GAGTACT	11775	0.0	15.966968	12-13
ATGGGGG	8865	0.0	15.958336	5
>>END_MODULE