FastQCFastQC Report
Fri 27 May 2016
SRR2140094_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140094_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3728213
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG131000.022.4324552
ACATGGG130850.021.9745483
GTACATG136400.021.9503171
GTACTAG13050.020.6090351
CATGGGG90950.019.0039484
AGAGTAC127850.016.72080410-11
GAGTACT71400.016.53504612-13
ATGGGGG61800.016.1918745
GTCTAGA14150.015.932271
GAGTACA97550.015.569091
AGTACAT94950.015.2456312
CGACGAG5800.015.1103824-25
AGTACTT78900.015.03846612-13
CAATGCG5800.015.02554118-19
TGCGACG5700.014.98302522-23
ATGGGCG5550.014.9655055
GTACTTT78750.014.94646214-15
CATGGGC30850.014.7437444
TATACTG15850.014.7227125
GGTTCAC11900.014.6241566
Produced by FastQC (version 0.11.5)