##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140094_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3728213
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.74392584329275	34.0	31.0	34.0	31.0	34.0
2	32.93927331941603	34.0	31.0	34.0	31.0	34.0
3	33.054383158902134	34.0	33.0	34.0	31.0	34.0
4	36.390145359184146	37.0	37.0	37.0	35.0	37.0
5	36.23890882843872	37.0	37.0	37.0	35.0	37.0
6	36.32101679812822	37.0	37.0	37.0	35.0	37.0
7	36.32601731714363	37.0	37.0	37.0	35.0	37.0
8	36.35535791544099	37.0	37.0	37.0	35.0	37.0
9	38.220403984429	39.0	39.0	39.0	37.0	39.0
10-11	38.144706056225864	39.0	38.0	39.0	37.0	39.0
12-13	38.136109846728175	39.0	38.5	39.0	37.0	39.0
14-15	39.57507510971074	41.0	39.0	41.0	37.0	41.0
16-17	39.57077143929277	41.0	39.0	41.0	37.0	41.0
18-19	39.60928372386449	41.0	39.5	41.0	37.0	41.0
20-21	39.6324904984611	41.0	39.5	41.0	37.0	41.0
22-23	39.58723285083779	41.0	39.0	41.0	37.0	41.0
24-25	39.55406213951498	41.0	39.0	41.0	37.0	41.0
26-27	39.43125070413094	41.0	39.0	41.0	37.0	41.0
28-29	39.365865328571324	40.0	39.0	41.0	36.0	41.0
30-31	39.31770866776425	40.0	39.0	41.0	36.0	41.0
32-33	39.230866598809854	40.0	39.0	41.0	36.0	41.0
34-35	39.18564528252863	40.0	39.0	41.0	35.5	41.0
36-37	39.14279240051974	40.0	39.0	41.0	35.0	41.0
38-39	39.077772810769275	40.0	39.0	41.0	35.0	41.0
40-41	39.0335137927789	40.0	39.0	41.0	35.0	41.0
42-43	39.01502444391471	40.0	38.0	41.0	35.0	41.0
44-45	38.95541923209025	40.0	38.0	41.0	35.0	41.0
46-47	38.84617907010106	40.0	38.0	41.0	35.0	41.0
48-49	38.83226274897268	40.0	38.0	41.0	35.0	41.0
50-51	38.72110554527151	40.0	38.0	41.0	35.0	41.0
52-53	38.56764315062749	40.0	38.0	41.0	35.0	41.0
54-55	38.37769092986877	40.0	37.5	41.0	34.5	41.0
56-57	38.22087098737582	40.0	37.0	41.0	34.0	41.0
58-59	38.06064002959572	39.5	36.5	41.0	34.0	41.0
60-61	37.80216059583347	39.0	36.0	41.0	34.0	41.0
62-63	37.640618005072106	39.0	35.5	41.0	34.0	41.0
64-65	37.35163493812184	39.0	35.0	40.0	33.0	41.0
66-67	37.097186755285044	38.0	35.0	40.0	33.0	41.0
68-69	36.82090902914781	37.0	35.0	40.0	33.0	41.0
70-71	36.35172887466798	37.0	35.0	39.0	33.0	41.0
72-73	36.08164846279574	36.0	35.0	39.0	33.0	41.0
74-75	35.73135629329076	36.0	35.0	38.5	32.0	40.0
76-77	33.97708302500952	34.5	33.0	36.5	30.0	39.0
78-79	34.97592552668209	35.0	34.0	37.0	31.5	39.0
80-81	34.9264951279575	35.0	34.0	36.5	32.0	39.0
82-83	34.6741617402068	35.0	34.0	36.0	32.0	37.5
84-85	34.45941640763401	35.0	34.0	36.0	32.0	37.0
86-87	34.31823662190122	35.0	34.0	35.5	32.0	37.0
88-89	34.173790779495434	35.0	34.0	35.0	31.0	36.5
90-91	34.12456824871902	35.0	34.0	35.0	32.0	36.0
92-93	34.02445044793532	35.0	34.0	35.0	31.5	36.0
94-95	33.891559386698106	35.0	34.0	35.0	31.0	36.0
96-97	33.928608078202515	35.0	34.0	35.0	31.0	35.5
98-99	33.965554169064625	35.0	34.0	35.0	31.0	35.0
100-101	32.73509742778117	34.0	32.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	6.0
19	12.0
20	27.0
21	89.0
22	214.0
23	607.0
24	1420.0
25	3058.0
26	5615.0
27	9628.0
28	14375.0
29	20295.0
30	28057.0
31	38273.0
32	51887.0
33	73371.0
34	118355.0
35	200978.0
36	415223.0
37	921486.0
38	1188273.0
39	620562.0
40	16402.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.904435449369444	19.21408460299881	12.398889226554385	23.48259072107736
2	15.293300022906731	21.53385502024858	39.09028094809185	24.08256400875284
3	20.433331151755223	25.752698093551075	30.188257673701003	23.625713080992696
4	12.430298376192562	16.963328007278555	39.60546245614186	31.00091116038703
5	12.763997121409105	38.6733000501849	35.34540542613848	13.21729740226752
6	29.7342439085101	37.48931452851287	18.376570097095296	14.399871465881727
7	26.372286132793377	32.961421463848765	23.078670666080505	17.587621737277352
8	24.905283040427143	33.66232025906245	20.93815455286487	20.494242147645533
9	25.272758094228546	16.971057439866616	21.940866023713244	35.81531844219159
10-11	25.10453433170583	25.47031396644119	28.125495379675076	21.29965632217791
12-13	27.01389880766778	23.29345481294915	28.031884040478005	21.66076233890506
14-15	23.256490441935586	24.835504301926957	27.150071629491123	24.75793362664633
16-17	21.85733486793807	27.774754285766395	27.625956456886986	22.741954389408544
18-19	21.520806348778894	28.70969282066234	28.04312682778586	21.72637400277291
20-21	23.05856493353953	26.703899432090537	27.221767496946867	23.015768137423063
22-23	22.51409222547346	26.443926179668427	27.715784098030444	23.32619749682767
24-25	23.412243228949567	26.4932338862762	27.568147084137255	22.526375800636977
26-27	23.05268060756002	25.957631567097017	28.79504897325206	22.194638852090897
28-29	22.025329077908104	27.785824636863865	28.14897761320233	22.0398686720257
30-31	22.317815167182694	27.0418886834323	27.73927437562662	22.90102177375838
32-33	22.326486523488192	26.96947138542537	27.763269963908616	22.94077212717782
34-35	23.153238511191596	26.86295821354363	27.38333095963612	22.60047231562865
36-37	22.91987415397427	26.314062827666618	28.385983660518853	22.38007935784026
38-39	22.77603755916716	26.906617479254813	28.085422931674398	22.231922029903632
40-41	22.48246351798681	27.184788992945858	28.24867408120813	22.084073407859204
42-43	22.275527438238914	27.437351501445622	27.001545816276874	23.28557524403859
44-45	22.38465192562753	27.23462425167751	26.871884864746292	23.50883895794867
46-47	23.31858321412689	27.157313930431126	26.879967745906796	22.644135109535192
48-49	23.140048332516177	26.900629332141246	27.191096097828282	22.7682262375143
50-51	22.226780722793002	27.034009568577606	27.88643900008883	22.852770708540564
52-53	22.595613878156055	27.494789766799954	26.813846557760986	23.09574979728301
54-55	22.44241351320983	27.103659997909872	26.935412772236848	23.518513716643447
56-57	23.089654026611374	27.034217130770816	26.25598867682791	23.6201401657899
58-59	23.318694987429062	26.93435613882265	26.731470191391082	23.01547868235721
60-61	23.31664999874495	27.09791226023624	27.2140867938537	22.371350947165112
62-63	22.84632022653738	27.283325669619124	27.01895268385955	22.85140141998395
64-65	22.427961722093904	27.753030680292824	26.775795948745174	23.043211648868102
66-67	22.639023213966237	27.415193700362746	26.306724435813067	23.639058649857954
68-69	22.688640986999065	27.24245712854626	26.685108458297268	23.38379342615741
70-71	23.341760404728216	26.582614017489508	26.418600710533674	23.6570248672486
72-73	22.971960928593766	26.689047426845608	27.378243509528055	22.960748135032567
74-75	22.716877167451564	27.475665183649607	26.89533328742178	22.912124361477055
76-77	22.510776135000228	27.140325680831285	26.803050244227787	23.545847939940696
78-79	22.622606861207025	27.050479726514805	26.836996172506634	23.489917239771536
80-81	22.96466085341523	27.102161934343442	26.524011408369617	23.40916580387171
82-83	22.982879170649102	26.69336384439359	27.32267429178779	23.001082693169515
84-85	23.213254923139363	26.914724871737246	26.745857028055305	23.12616317706809
86-87	22.670277227213056	27.38220288810523	27.146624108728666	22.800895775953048
88-89	22.902877379222737	27.00558700962541	26.59500959854047	23.496526012611387
90-91	22.686660246002667	26.939204364509532	26.830194425330227	23.543940964157574
92-93	23.188986700236974	26.986507369526375	26.869890352013705	22.95461557822295
94-95	22.736757869762165	26.74651335554925	27.237434143887835	23.27929463080074
96-97	22.60871194413637	26.95693837626792	27.42014569107296	23.01420398852275
98-99	23.177429093818237	26.621552367147633	27.450465402799402	22.75055313623473
100-101	26.483691968783056	34.40563603697243	28.738992317286282	10.371679676958227
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	456.0
1	514.5
2	1006.5
3	1537.0
4	2283.0
5	4021.5
6	5322.5
7	5857.5
8	5841.5
9	5218.5
10	4398.5
11	3702.0
12	4074.0
13	5773.0
14	9449.0
15	14078.0
16	17587.0
17	19510.5
18	19607.0
19	18240.5
20	16237.0
21	14476.5
22	13612.5
23	14445.0
24	17096.0
25	21139.5
26	27112.0
27	33803.0
28	40064.5
29	47087.0
30	53158.5
31	58366.0
32	65533.5
33	73483.5
34	81939.5
35	90943.0
36	99367.5
37	107170.5
38	112331.0
39	115899.5
40	120409.0
41	123999.0
42	128391.5
43	139933.0
44	157656.0
45	184348.5
46	220591.5
47	258155.0
48	273199.0
49	250502.5
50	213479.0
51	184098.5
52	165365.0
53	155537.0
54	148498.5
55	138656.5
56	129281.0
57	117137.0
58	103627.0
59	90192.0
60	74302.0
61	59920.5
62	48007.0
63	37196.0
64	27309.0
65	20064.0
66	14906.0
67	11570.0
68	9022.5
69	6707.0
70	4984.5
71	3373.0
72	2365.5
73	1649.0
74	1084.5
75	722.5
76	479.5
77	295.0
78	204.0
79	166.5
80	131.5
81	88.0
82	63.0
83	49.5
84	37.5
85	26.5
86	17.5
87	13.0
88	9.5
89	8.0
90	9.0
91	8.0
92	6.5
93	6.0
94	3.5
95	1.0
96	1.0
97	2.0
98	3.0
99	4.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0013679475931230325
3	6.705625456485453E-4
4	0.0
5	0.0
6	5.364500365188363E-4
7	0.0
8	0.0
9	2.6822501825941812E-5
10-11	3.2187002191130175E-4
12-13	4.157487783020981E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	8.06530639897377E-5
28-29	0.0
30-31	5.405563919914949E-5
32-33	2.1731114133848725E-4
34-35	2.7336236806165304E-5
36-37	7.302477289640086E-4
38-39	1.9517300902270878E-4
40-41	2.825160426734832E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	1.5262834402978207E-5
52-53	0.0
54-55	6.600351987342411E-4
56-57	7.987558578757728E-5
58-59	0.0
60-61	0.0010238446821011803
62-63	0.0
64-65	0.0
66-67	6.91431555449613E-5
68-69	0.0
70-71	0.0
72-73	1.272114344906545E-4
74-75	0.0
76-77	3.752298048035794E-5
78-79	7.643515604428194E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	3.088873055554411E-4
88-89	1.0494785036367578E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	524.0
20-21	2519.0
22-23	2618.0
24-25	2252.0
26-27	7932.0
28-29	8699.0
30-31	17163.0
32-33	21903.0
34-35	26153.0
36-37	39623.0
38-39	48479.0
40-41	45589.0
42-43	49364.0
44-45	57748.0
46-47	55514.0
48-49	52269.0
50-51	50648.0
52-53	44519.0
54-55	51730.0
56-57	52602.0
58-59	50468.0
60-61	47194.0
62-63	43395.0
64-65	44941.0
66-67	46944.0
68-69	48267.0
70-71	47306.0
72-73	43726.0
74-75	42324.0
76-77	45955.0
78-79	50878.0
80-81	46033.0
82-83	47077.0
84-85	47032.0
86-87	45579.0
88-89	46384.0
90-91	48494.0
92-93	45861.0
94-95	50004.0
96-97	74123.0
98-99	314733.0
100-101	1813647.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.18887463481258
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.21217902759199	75.10672956052396
2	7.220704917053805	12.158060420736348
3	1.9930468823123388	5.033771223488927
4	0.7581945223515056	2.5532617436421
5	0.329834758080378	1.3884208549116337
6	0.173353923156657	0.8756683022453237
7	0.1002789150402661	0.5909658304787988
8	0.0602389506357054	0.4057159570561652
9	0.04157721221970464	0.315030483650674
>10	0.10896713832630764	1.3383039678573327
>50	7.132659543050377E-4	0.03506038277473037
>100	8.59962999787835E-4	0.17625460869609128
>500	5.052427726240549E-5	0.02275666393785654
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	5.3645003651883624E-5	0.0	0.0	0.0	0.0
3	5.3645003651883624E-5	0.0	0.0	0.0	0.0
4	5.3645003651883624E-5	0.0	0.0	0.0	0.0
5	5.3645003651883624E-5	0.0	0.0	0.0	0.0
6	5.3645003651883624E-5	0.0	0.0	0.0	0.0
7	5.3645003651883624E-5	0.0	0.0	0.0	0.0
8	5.3645003651883624E-5	0.0	0.0	0.0	0.0
9	5.3645003651883624E-5	0.0	0.0	2.6822501825941812E-5	0.0
10-11	5.3645003651883624E-5	0.0	0.0	8.046750547782544E-5	0.0
12-13	5.3645003651883624E-5	0.0	0.0	1.3411250912970905E-4	0.0
14-15	5.3645003651883624E-5	0.0	0.0	2.145800146075345E-4	0.0
16-17	6.705625456485454E-5	0.0	0.0	4.962162837799234E-4	0.0
18-19	9.387875639079634E-5	0.0	0.0	8.985538111690506E-4	0.0
20-21	1.0729000730376725E-4	0.0	0.0	0.0013813588440360033	0.0
22-23	1.6093501095565087E-4	0.0	0.0	0.002186033898814258	0.0
24-25	1.6093501095565087E-4	0.0	0.0	0.0027761289389849775	0.0
26-27	1.7434626186862176E-4	0.0	0.0	0.003285756473677872	0.0
28-29	2.279912655205054E-4	0.0	0.0	0.004720760321365759	0.0
30-31	2.682250182594181E-4	0.0	0.0	0.006893382969267045	0.0
32-33	2.81636269172389E-4	0.0	0.0	0.011064282003200997	0.0
34-35	2.950475200853599E-4	0.0	0.0	0.015758219822740817	0.0
36-37	3.4869252373724357E-4	2.6822501825941812E-5	0.0	0.020948373926060554	0.0
38-39	3.4869252373724357E-4	2.6822501825941812E-5	0.0	0.0297327432740565	0.0
40-41	3.4869252373724357E-4	2.6822501825941812E-5	0.0	0.039952116469740326	0.0
42-43	3.8892627647615626E-4	2.6822501825941812E-5	0.0	0.0482134470321304	0.0
44-45	4.559825310410108E-4	2.6822501825941812E-5	0.0	0.05701122763103932	0.0
46-47	5.498612874318071E-4	2.6822501825941812E-5	0.0	0.06611746700094656	0.0
48-49	5.900950401707198E-4	2.6822501825941812E-5	0.0	0.07510300511263707	0.0
50-51	5.900950401707198E-4	2.6822501825941812E-5	0.0	0.085282144555582	0.0
52-53	5.900950401707198E-4	2.6822501825941812E-5	0.0	0.09467002019466163	0.0
54-55	5.900950401707198E-4	2.6822501825941812E-5	0.0	0.10415177459013206	0.0
56-57	6.169175419966617E-4	2.6822501825941812E-5	0.0	0.1137676414947322	0.0
58-59	6.437400438226035E-4	2.6822501825941812E-5	0.0	0.12287388086463945	0.0
60-61	6.705625456485453E-4	2.6822501825941812E-5	0.0	0.13181918522359104	0.0
62-63	6.705625456485453E-4	2.6822501825941812E-5	0.0	0.13991958077502548	0.0
64-65	6.705625456485453E-4	2.6822501825941812E-5	0.0	0.14693366500250926	0.0
66-67	6.973850474744871E-4	2.6822501825941812E-5	0.0	0.15421597424825245	0.0
68-69	6.973850474744871E-4	2.6822501825941812E-5	0.0	0.16200791102868856	0.0
70-71	7.510300511263707E-4	2.6822501825941812E-5	0.0	0.16965232404908195	0.0
72-73	7.912638038652834E-4	2.6822501825941812E-5	0.0	0.17595561197817827	0.0
74-75	8.046750547782543E-4	2.6822501825941812E-5	0.0	0.18099824232145534	0.0
76-77	8.180863056912252E-4	2.6822501825941812E-5	0.0	0.1854910113773006	0.0
78-79	8.314975566041962E-4	2.6822501825941812E-5	0.0	0.1896753216621475	0.0
80-81	8.58320058430138E-4	2.6822501825941812E-5	0.0	0.191298083022617	0.0
82-83	8.58320058430138E-4	2.6822501825941812E-5	0.0	0.19200887932100447	0.0
84-85	8.717313093431089E-4	2.6822501825941812E-5	0.0	0.19269285311756595	0.0
86-87	8.985538111690507E-4	2.6822501825941812E-5	0.0	0.19294766688491244	0.0
88-89	9.119650620820216E-4	2.6822501825941812E-5	0.0	0.1930147231394773	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACATGG	13100	0.0	22.432455	2
ACATGGG	13085	0.0	21.974548	3
GTACATG	13640	0.0	21.950317	1
GTACTAG	1305	0.0	20.609035	1
CATGGGG	9095	0.0	19.003948	4
AGAGTAC	12785	0.0	16.720804	10-11
GAGTACT	7140	0.0	16.535046	12-13
ATGGGGG	6180	0.0	16.191874	5
GTCTAGA	1415	0.0	15.93227	1
GAGTACA	9755	0.0	15.56909	1
AGTACAT	9495	0.0	15.245631	2
CGACGAG	580	0.0	15.11038	24-25
AGTACTT	7890	0.0	15.038466	12-13
CAATGCG	580	0.0	15.025541	18-19
TGCGACG	570	0.0	14.983025	22-23
ATGGGCG	555	0.0	14.965505	5
GTACTTT	7875	0.0	14.946462	14-15
CATGGGC	3085	0.0	14.743744	4
TATACTG	1585	0.0	14.722712	5
GGTTCAC	1190	0.0	14.624156	6
>>END_MODULE