FastQCFastQC Report
Fri 27 May 2016
SRR2140093_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140093_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4414041
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGG112600.023.1735742
GGTATCA81000.023.1360721
CAACGCA101450.022.6144125
ATCAACG101700.022.600143
TCAACGC102750.022.3282954
ACATGGG113150.022.169943
GTACATG119900.022.113121
AACGCAG105700.021.9041446
GTATCAA114150.021.2760871
CATGGGG87100.019.0074044
ACGCAGA121700.018.9205937
TATCAAC132400.018.216452
CGCAGAG126000.018.1748458
ATTATAC24150.017.5731243
ACTATCG3750.017.36761910-11
AGAGTAC105950.017.13257410-11
GAGTACT66850.016.90784512-13
GAGTACA78150.016.344981
GTATAAT25550.016.2811151
CAGAGTA116950.016.1139510-11
Produced by FastQC (version 0.11.5)