##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140093_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4414041
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.809285867530456	34.0	31.0	34.0	31.0	34.0
2	33.007989051302424	34.0	33.0	34.0	31.0	34.0
3	33.11575810011733	34.0	33.0	34.0	31.0	34.0
4	36.47494710629104	37.0	37.0	37.0	35.0	37.0
5	36.341577253133806	37.0	37.0	37.0	35.0	37.0
6	36.36928361109469	37.0	37.0	37.0	35.0	37.0
7	36.37375525057425	37.0	37.0	37.0	35.0	37.0
8	36.385349841562416	37.0	37.0	37.0	35.0	37.0
9	38.283975160176354	39.0	39.0	39.0	37.0	39.0
10-11	38.19528771934832	39.0	39.0	39.0	37.0	39.0
12-13	38.180425374390495	39.0	39.0	39.0	37.0	39.0
14-15	39.706059821374566	41.0	40.0	41.0	38.0	41.0
16-17	39.68086080306006	41.0	40.0	41.0	38.0	41.0
18-19	39.71319308089798	41.0	40.0	41.0	38.0	41.0
20-21	39.727373558120945	41.0	40.0	41.0	38.0	41.0
22-23	39.68084176476869	41.0	40.0	41.0	37.5	41.0
24-25	39.66197024869244	41.0	40.0	41.0	37.0	41.0
26-27	39.55435286774518	41.0	39.5	41.0	37.0	41.0
28-29	39.48856431734424	41.0	39.0	41.0	37.0	41.0
30-31	39.432370351471704	40.5	39.0	41.0	37.0	41.0
32-33	39.35242404131025	40.0	39.0	41.0	36.0	41.0
34-35	39.319300456311	40.0	39.0	41.0	36.0	41.0
36-37	39.28145282124704	40.0	39.0	41.0	36.0	41.0
38-39	39.21992987537945	40.0	39.0	41.0	36.0	41.0
40-41	39.1571279830071	40.0	39.0	41.0	35.0	41.0
42-43	39.1453197590327	40.0	39.0	41.0	35.0	41.0
44-45	39.0861856307822	40.0	39.0	41.0	35.0	41.0
46-47	38.97658843315083	40.0	38.5	41.0	35.0	41.0
48-49	38.963081102968445	40.0	38.5	41.0	35.0	41.0
50-51	38.85856679836982	40.0	38.0	41.0	35.0	41.0
52-53	38.69594298553838	40.0	38.0	41.0	35.0	41.0
54-55	38.496966395757454	40.0	38.0	41.0	34.5	41.0
56-57	38.354550778861935	40.0	37.0	41.0	34.0	41.0
58-59	38.196495634094106	40.0	37.0	41.0	34.0	41.0
60-61	37.96225227625963	39.5	36.5	41.0	34.0	41.0
62-63	37.79584687987713	39.0	36.0	41.0	34.0	41.0
64-65	37.51546927768901	39.0	35.5	40.5	33.5	41.0
66-67	37.26404915194499	38.5	35.0	40.0	33.0	41.0
68-69	36.98762996068092	37.5	35.0	40.0	33.0	41.0
70-71	36.529725965197116	37.0	35.0	39.5	33.0	41.0
72-73	36.255465176209555	36.5	35.0	39.0	33.0	41.0
74-75	35.88623411665341	36.0	35.0	39.0	32.5	40.5
76-77	34.096067962301305	34.5	33.0	36.5	30.0	39.0
78-79	35.10140029220899	35.0	34.0	37.0	31.5	39.0
80-81	35.02630494487357	35.0	34.0	37.0	32.0	39.0
82-83	34.76128905870369	35.0	34.0	36.0	32.0	38.5
84-85	34.52399775356952	35.0	34.0	36.0	32.0	37.0
86-87	34.33900946490126	35.0	34.0	36.0	31.5	37.0
88-89	34.178949041506655	35.0	34.0	35.0	31.0	36.5
90-91	34.11880515375867	35.0	34.0	35.0	31.5	36.0
92-93	33.99932378983377	35.0	34.0	35.0	31.0	36.0
94-95	33.8530960256208	35.0	34.0	35.0	31.0	36.0
96-97	33.88296916966879	35.0	34.0	35.0	31.0	35.5
98-99	33.91681517556715	35.0	34.0	35.0	31.0	35.0
100-101	32.68226229248198	34.0	31.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	12.0
20	18.0
21	46.0
22	185.0
23	523.0
24	1581.0
25	3578.0
26	6715.0
27	11352.0
28	16942.0
29	24462.0
30	33991.0
31	45353.0
32	61258.0
33	86291.0
34	135973.0
35	230703.0
36	485024.0
37	1096262.0
38	1471141.0
39	685134.0
40	17495.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.37284134877768	19.30910927198003	12.511347311907613	24.806702067334673
2	15.343652829932203	20.740760878799723	40.071364246512495	23.84422204475558
3	18.7592814989863	25.437089929073775	29.915883990636203	25.887744581303725
4	12.473354914465	17.11882603718452	38.741710826881764	31.66610822146872
5	13.27223738972973	37.69797335366844	35.69339297029638	13.336396286305451
6	30.911546723685063	37.527520302382364	18.3563531968861	13.20457977704647
7	27.090573014614044	32.18937023919805	22.92108297136343	17.798973774824475
8	25.272964161411277	35.85317852734037	20.95392861099387	17.919928700254484
9	25.96268406328082	15.994965155495908	20.635975350467	37.40637543075628
10-11	24.68506090129016	26.13958271220753	28.80021377299827	20.37514261350404
12-13	25.432681029161742	23.529664968630065	28.88358331901343	22.154070683194764
14-15	22.38334442294487	25.02930987727572	26.316667652158195	26.270678047621214
16-17	22.401649644849243	28.065643250708366	26.897835792644425	22.634871311797966
18-19	22.23758229703802	27.60496560861125	28.236620366688935	21.920831727661795
20-21	23.06842763205966	26.951935205959206	27.97392877028283	22.005708391698306
22-23	22.82997425740779	26.718839226634515	27.735105100477686	22.716081415479998
24-25	22.581799661688244	26.819366319371646	27.99053787851829	22.608296140421817
26-27	22.617224977476845	27.009690482503927	27.848038124808483	22.525046415210745
28-29	22.4694470783985	27.27241786090862	27.86154621460527	22.39658884608761
30-31	22.813073622827904	27.095885416808724	27.66657620529549	22.424464755067884
32-33	22.524686275906276	27.254263941314722	27.698038601239226	22.523011181539776
34-35	22.59123053027719	27.284455290290733	27.652315029271552	22.471999150160528
36-37	22.6558732575464	27.108482059330424	27.69788108264159	22.537763600481586
38-39	22.491916036312215	27.27423460984331	27.800683676327438	22.433165677517035
40-41	22.787132077965413	27.12091149415652	27.694436589411282	22.39751983846679
42-43	22.65055960415211	27.28528180806199	27.48665736818412	22.57750121960178
44-45	22.71733268109343	27.48566034892458	27.26872640414011	22.528280565841882
46-47	22.723574144441013	27.26972821515872	27.137565449161787	22.869132191238478
48-49	22.73436004015257	27.204411780241045	27.349076635338804	22.712151544267577
50-51	22.587764197926298	27.429538939173554	27.20918002267257	22.77351684022758
52-53	22.782897284607635	27.413302659637772	26.99308739637813	22.81071265937646
54-55	22.855692190282625	27.274710650723932	27.020593896796967	22.84900326219648
56-57	22.832070908825646	27.300188400515623	27.04741718240492	22.82032350825381
58-59	22.754408913602937	27.429125065333555	27.138376398367804	22.67808962269571
60-61	22.907129599842257	27.335709526801473	27.030729856843827	22.726431016512443
62-63	22.782646204496178	27.366044953419756	27.019164116545287	22.832144725538786
64-65	22.691128759248695	27.463963507082163	26.988833725652402	22.856074008016744
66-67	22.760927895706462	27.447245639979013	26.859212486124985	22.932613978189544
68-69	22.800713201416382	27.43249644463008	26.997234607557445	22.76955574639609
70-71	22.8178643413366	27.108860625683505	26.909142228487326	23.164132804492567
72-73	22.878464119451987	27.197384011203035	27.034484165186868	22.889667704158114
74-75	22.642119335586315	27.42981027997863	26.985010077112726	22.94306030732233
76-77	22.735995996058055	27.262341330978163	26.910404190161312	23.091258482802466
78-79	22.856677367962906	27.293009169057626	26.887587641421103	22.96272582155837
80-81	22.733607845960872	27.33871902155492	26.86887214673213	23.058800985752075
82-83	22.89805661128807	27.254091594564038	26.986831451520764	22.86102034262712
84-85	23.101250194072115	27.176261838398926	26.849392646700032	22.87309532082893
86-87	22.67877712360385	27.298707327647552	26.966429010023724	23.056086538724873
88-89	22.900716156982874	27.116470155680105	27.000621677729658	22.98219200960736
90-91	22.94808433071685	27.245605169642133	26.90407311050757	22.902237389133447
92-93	22.957968042077315	27.238642025972887	26.900549703951015	22.902840227998787
94-95	22.75161507538706	27.377378945289955	26.925211630567958	22.945794348755026
96-97	22.715839405732392	27.301598439654835	27.048455966100892	22.93410618851188
98-99	23.136436980716475	27.80626394957555	27.071673347421832	21.985625722286148
100-101	27.355520480585398	35.03792195040927	27.51741274221769	10.08914482678764
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	527.0
1	510.0
2	808.0
3	1212.0
4	1761.5
5	3033.5
6	4054.0
7	4586.0
8	4789.5
9	4457.5
10	3853.5
11	3407.5
12	3827.5
13	5314.5
14	8358.5
15	12203.5
16	15188.5
17	16810.0
18	17103.5
19	16399.0
20	15320.5
21	14572.0
22	14999.0
23	17222.0
24	21296.0
25	27361.5
26	35543.5
27	44487.5
28	52428.0
29	60086.0
30	66087.5
31	71647.0
32	79172.5
33	87017.0
34	96554.5
35	107796.0
36	119789.0
37	129644.0
38	135780.5
39	141139.5
40	149853.5
41	158684.5
42	165320.0
43	172469.5
44	180916.5
45	190589.0
46	197103.0
47	200354.0
48	201917.0
49	202081.5
50	201332.0
51	199580.5
52	192603.5
53	182979.5
54	174748.5
55	163236.0
56	149805.0
57	136606.5
58	123714.0
59	109131.5
60	91390.0
61	73915.0
62	58597.5
63	44956.5
64	33842.5
65	25233.0
66	18505.5
67	13580.0
68	9732.0
69	6963.5
70	5076.5
71	3639.0
72	2467.5
73	1684.5
74	1116.0
75	760.5
76	561.5
77	384.0
78	258.0
79	175.0
80	115.0
81	75.5
82	53.0
83	38.0
84	26.0
85	14.0
86	7.5
87	6.5
88	5.0
89	4.0
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	1.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0014952285218918448
3	4.07789596879594E-4
4	0.0
5	0.0
6	6.11684395319391E-4
7	0.0
8	0.0
9	4.5309955208843774E-5
10-11	2.945147088574846E-4
12-13	4.870820184950706E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	3.401003477412689E-5
28-29	1.1345098730154444E-5
30-31	4.545795480115554E-5
32-33	3.0766944723879206E-4
34-35	6.860916753409618E-5
36-37	7.929845006007719E-4
38-39	1.38944289708107E-4
40-41	4.670207462290994E-5
42-43	0.0
44-45	0.0
46-47	1.2013703310544138E-5
48-49	0.0
50-51	1.8401033598324575E-4
52-53	0.0
54-55	6.001245258391117E-4
56-57	1.1368439242134366E-4
58-59	0.0
60-61	8.516749930801408E-4
62-63	0.0
64-65	0.0
66-67	3.9942461552717925E-5
68-69	0.0
70-71	0.0
72-73	1.5158578715123038E-4
74-75	0.0
76-77	1.409044686020459E-5
78-79	1.2828425510321892E-4
80-81	0.0
82-83	0.0
84-85	0.0
86-87	6.285457650905981E-4
88-89	9.093278854489352E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	177.0
20-21	744.0
22-23	969.0
24-25	1236.0
26-27	2515.0
28-29	5924.0
30-31	11388.0
32-33	14178.0
34-35	19321.0
36-37	30201.0
38-39	36429.0
40-41	34538.0
42-43	38573.0
44-45	44436.0
46-47	44725.0
48-49	42823.0
50-51	39135.0
52-53	37599.0
54-55	40237.0
56-57	42160.0
58-59	41571.0
60-61	40985.0
62-63	38592.0
64-65	39739.0
66-67	41944.0
68-69	42697.0
70-71	42840.0
72-73	40106.0
74-75	39889.0
76-77	40017.0
78-79	42404.0
80-81	43198.0
82-83	41335.0
84-85	40196.0
86-87	41273.0
88-89	44122.0
90-91	46211.0
92-93	47223.0
94-95	50476.0
96-97	78288.0
98-99	361590.0
100-101	2682037.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.59959740507004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.96766264507458	77.01195701893543
2	7.117499650080101	12.185102091551672
3	1.6916042366667388	4.344019248821509
4	0.5840654830779222	1.9998308083867156
5	0.25734790455167716	1.1014438511330982
6	0.14103165315019722	0.7243351638617015
7	0.0702951215345036	0.42120638720257836
8	0.04367998020354669	0.29911909760680205
9	0.032132867090554164	0.24755044377798543
>10	0.09023866082257179	1.191643728262747
>50	0.002865986417725528	0.16580927726435998
>100	0.001534632353974695	0.2657897852942658
>500	0.0	0.0
>1k	4.1178975812924155E-5	0.04219309790113891
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.265497760442189E-5	0.0	0.0	0.0	0.0
3	2.265497760442189E-5	0.0	0.0	0.0	2.265497760442189E-5
4	2.265497760442189E-5	0.0	0.0	0.0	2.265497760442189E-5
5	2.265497760442189E-5	2.265497760442189E-5	0.0	0.0	2.265497760442189E-5
6	2.265497760442189E-5	2.265497760442189E-5	0.0	0.0	2.265497760442189E-5
7	2.265497760442189E-5	2.265497760442189E-5	0.0	0.0	2.265497760442189E-5
8	2.265497760442189E-5	2.265497760442189E-5	0.0	0.0	2.265497760442189E-5
9	2.265497760442189E-5	2.265497760442189E-5	0.0	0.0	2.265497760442189E-5
10-11	2.265497760442189E-5	2.265497760442189E-5	0.0	2.265497760442189E-5	2.265497760442189E-5
12-13	2.265497760442189E-5	2.265497760442189E-5	0.0	5.663744401105472E-5	2.265497760442189E-5
14-15	2.265497760442189E-5	2.265497760442189E-5	0.0	1.1327488802210945E-4	2.265497760442189E-5
16-17	2.265497760442189E-5	2.265497760442189E-5	0.0	1.5858484323095324E-4	2.265497760442189E-5
18-19	2.265497760442189E-5	2.265497760442189E-5	0.0	2.265497760442189E-4	2.265497760442189E-5
20-21	2.265497760442189E-5	4.530995520884378E-5	0.0	3.8513461927517213E-4	2.265497760442189E-5
22-23	2.265497760442189E-5	4.530995520884378E-5	0.0	6.569943505282348E-4	2.265497760442189E-5
24-25	2.265497760442189E-5	4.530995520884378E-5	0.0	9.515090593857193E-4	2.265497760442189E-5
26-27	2.265497760442189E-5	4.530995520884378E-5	0.0	0.0012346962794409929	2.265497760442189E-5
28-29	2.265497760442189E-5	4.530995520884378E-5	0.0	0.0018916906299692277	2.265497760442189E-5
30-31	2.265497760442189E-5	4.530995520884378E-5	0.0	0.0032849717526411736	2.265497760442189E-5
32-33	2.265497760442189E-5	4.530995520884378E-5	0.0	0.006060206509182855	2.265497760442189E-5
34-35	2.265497760442189E-5	4.530995520884378E-5	0.0	0.009617037993077091	2.265497760442189E-5
36-37	2.265497760442189E-5	4.530995520884378E-5	0.0	0.014691752976467593	2.265497760442189E-5
38-39	2.265497760442189E-5	4.530995520884378E-5	0.0	0.02171479603383838	2.265497760442189E-5
40-41	2.265497760442189E-5	4.530995520884378E-5	0.0	0.029440143396946245	2.265497760442189E-5
42-43	2.265497760442189E-5	4.530995520884378E-5	0.0	0.03704088838322979	2.265497760442189E-5
44-45	2.265497760442189E-5	4.530995520884378E-5	0.0	0.04510606041040398	2.265497760442189E-5
46-47	2.265497760442189E-5	4.530995520884378E-5	0.0	0.05429265382899706	2.265497760442189E-5
48-49	2.265497760442189E-5	4.530995520884378E-5	0.0	0.06275428796424863	2.265497760442189E-5
50-51	2.265497760442189E-5	4.530995520884378E-5	0.0	0.07112530218908253	2.265497760442189E-5
52-53	2.265497760442189E-5	5.663744401105472E-5	0.0	0.07993808847720263	2.265497760442189E-5
54-55	2.265497760442189E-5	9.061991041768756E-5	0.0	0.08795795054916798	2.265497760442189E-5
56-57	2.265497760442189E-5	1.1327488802210945E-4	0.0	0.09590984768832006	2.265497760442189E-5
58-59	2.265497760442189E-5	1.3592986562653133E-4	0.0	0.10356723011861466	2.265497760442189E-5
60-61	2.265497760442189E-5	1.3592986562653133E-4	0.0	0.11057894568718324	2.265497760442189E-5
62-63	2.265497760442189E-5	1.3592986562653133E-4	0.0	0.1172621640804877	2.265497760442189E-5
64-65	2.265497760442189E-5	1.3592986562653133E-4	0.0	0.12318644072404403	2.265497760442189E-5
66-67	2.265497760442189E-5	1.3592986562653133E-4	0.0	0.1297790392069308	2.265497760442189E-5
68-69	2.265497760442189E-5	1.3592986562653133E-4	0.0	0.13626969029059766	2.265497760442189E-5
70-71	2.265497760442189E-5	1.4725735442874227E-4	0.0	0.14291892621749547	2.265497760442189E-5
72-73	2.265497760442189E-5	1.5858484323095321E-4	0.0	0.14817488102172138	4.530995520884378E-5
74-75	2.265497760442189E-5	1.5858484323095321E-4	0.0	0.1525472916993748	4.530995520884378E-5
76-77	2.265497760442189E-5	1.5858484323095321E-4	0.0	0.15561704116477396	4.530995520884378E-5
78-79	2.265497760442189E-5	1.5858484323095321E-4	0.0	0.15881139300699745	4.530995520884378E-5
80-81	2.265497760442189E-5	1.8123982083537512E-4	0.0	0.16043122390571363	5.663744401105472E-5
82-83	2.265497760442189E-5	1.8123982083537512E-4	0.0	0.16086166848019762	6.796493281326567E-5
84-85	2.265497760442189E-5	1.8123982083537512E-4	0.0	0.161258130588275	7.929242161547662E-5
86-87	2.265497760442189E-5	1.8123982083537512E-4	0.0	0.1613827329650993	9.061991041768756E-5
88-89	2.265497760442189E-5	1.8123982083537512E-4	0.0	0.16139406045390153	9.061991041768756E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACATGG	11260	0.0	23.173574	2
GGTATCA	8100	0.0	23.136072	1
CAACGCA	10145	0.0	22.614412	5
ATCAACG	10170	0.0	22.60014	3
TCAACGC	10275	0.0	22.328295	4
ACATGGG	11315	0.0	22.16994	3
GTACATG	11990	0.0	22.11312	1
AACGCAG	10570	0.0	21.904144	6
GTATCAA	11415	0.0	21.276087	1
CATGGGG	8710	0.0	19.007404	4
ACGCAGA	12170	0.0	18.920593	7
TATCAAC	13240	0.0	18.21645	2
CGCAGAG	12600	0.0	18.174845	8
ATTATAC	2415	0.0	17.573124	3
ACTATCG	375	0.0	17.367619	10-11
AGAGTAC	10595	0.0	17.132574	10-11
GAGTACT	6685	0.0	16.907845	12-13
GAGTACA	7815	0.0	16.34498	1
GTATAAT	2555	0.0	16.281115	1
CAGAGTA	11695	0.0	16.11395	10-11
>>END_MODULE