FastQCFastQC Report
Fri 27 May 2016
SRR2140092_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140092_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6189112
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC97750.049.1572651
TATACAC109950.045.150163
TTATACA118250.041.118632
TACACAT155450.031.8029485
GGTATCA113250.030.8577021
ATACACA167300.030.623994
ACACATC167700.028.8469096
ACATCTC180350.026.8009098
CACATCT182200.026.5967
GTATCAA166900.026.540031
TACATGG206350.024.6711852
CAACGCA174250.024.5528815
ATCAACG175100.024.3874573
ACATGGG206850.024.1572153
GTACATG213600.024.0822641
TCAACGC181200.023.6787344
CATCTCC205900.023.415729
AACGCAG185500.023.2178786
CATGGGG151350.021.9291674
TATCAAC205450.021.2620752
Produced by FastQC (version 0.11.5)