##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140092_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6189112
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76972560845562	34.0	31.0	34.0	31.0	34.0
2	32.9797171225856	34.0	33.0	34.0	31.0	34.0
3	33.08614547612	34.0	33.0	34.0	31.0	34.0
4	36.45565034208462	37.0	37.0	37.0	35.0	37.0
5	36.31543151909353	37.0	37.0	37.0	35.0	37.0
6	36.342206604113805	37.0	37.0	37.0	35.0	37.0
7	36.34804993026463	37.0	37.0	37.0	35.0	37.0
8	36.36387190924966	37.0	37.0	37.0	35.0	37.0
9	38.27406161013082	39.0	39.0	39.0	37.0	39.0
10-11	38.18286872171646	39.0	39.0	39.0	37.0	39.0
12-13	38.16621495943198	39.0	39.0	39.0	37.0	39.0
14-15	39.66167812119089	41.0	40.0	41.0	37.5	41.0
16-17	39.63176066291901	41.0	40.0	41.0	37.0	41.0
18-19	39.6815837231577	41.0	40.0	41.0	37.5	41.0
20-21	39.70526108251205	41.0	40.0	41.0	37.5	41.0
22-23	39.64620059512824	41.0	40.0	41.0	37.0	41.0
24-25	39.62014232416558	41.0	40.0	41.0	37.0	41.0
26-27	39.50750535949882	41.0	39.0	41.0	37.0	41.0
28-29	39.4456978654909	41.0	39.0	41.0	37.0	41.0
30-31	39.384104638248154	40.0	39.0	41.0	36.5	41.0
32-33	39.28639626925953	40.0	39.0	41.0	36.0	41.0
34-35	39.22489761940207	40.0	39.0	41.0	36.0	41.0
36-37	39.20004807787498	40.0	39.0	41.0	35.5	41.0
38-39	39.11362485577829	40.0	39.0	41.0	35.0	41.0
40-41	39.074881210247455	40.0	39.0	41.0	35.0	41.0
42-43	39.067734342820856	40.0	39.0	41.0	35.0	41.0
44-45	39.0023458344412	40.0	39.0	41.0	35.0	41.0
46-47	38.89559618775636	40.0	38.0	41.0	35.0	41.0
48-49	38.88529047453849	40.0	38.0	41.0	35.0	41.0
50-51	38.74003370195693	40.0	38.0	41.0	35.0	41.0
52-53	38.57863653738602	40.0	38.0	41.0	35.0	41.0
54-55	38.347297094417684	40.0	37.5	41.0	34.5	41.0
56-57	38.17615169395566	40.0	37.0	41.0	34.0	41.0
58-59	38.00723267568813	39.5	36.5	41.0	34.0	41.0
60-61	37.73236861409454	39.0	36.0	41.0	33.5	41.0
62-63	37.560133168429715	39.0	35.5	41.0	33.5	41.0
64-65	37.30720589796629	38.5	35.0	40.0	33.0	41.0
66-67	37.07803440359359	38.0	35.0	40.0	33.0	41.0
68-69	36.82657494116907	37.0	35.0	40.0	33.0	41.0
70-71	36.387629352638015	37.0	35.0	39.0	33.0	41.0
72-73	36.12754956424356	36.0	35.0	39.0	33.0	41.0
74-75	35.7764629176102	36.0	35.0	39.0	32.5	40.0
76-77	34.016041470412006	34.5	33.0	36.5	30.0	39.0
78-79	35.02798368404767	35.0	34.0	37.0	31.5	39.0
80-81	34.972224185960236	35.0	34.0	37.0	32.0	39.0
82-83	34.724490104279695	35.0	34.0	36.0	32.0	38.0
84-85	34.49966123206832	35.0	34.0	36.0	32.0	37.0
86-87	34.33324117165961	35.0	34.0	35.5	32.0	37.0
88-89	34.1822926064908	35.0	34.0	35.0	31.5	36.5
90-91	34.12859654759558	35.0	34.0	35.0	32.0	36.0
92-93	34.016295138701864	35.0	34.0	35.0	31.5	36.0
94-95	33.87728301258205	35.0	34.0	35.0	31.0	36.0
96-97	33.907886139587625	35.0	34.0	35.0	31.0	35.5
98-99	33.94045247423425	35.0	34.0	35.0	31.0	35.0
100-101	32.68630360952286	34.0	31.5	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	7.0
19	13.0
20	27.0
21	89.0
22	310.0
23	916.0
24	2357.0
25	5263.0
26	9684.0
27	16077.0
28	23853.0
29	34245.0
30	46531.0
31	61940.0
32	84591.0
33	120493.0
34	195253.0
35	332292.0
36	707566.0
37	1573976.0
38	1988835.0
39	960828.0
40	23964.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.437218780335535	19.87808590311502	12.72069401878654	24.964001297762913
2	15.174046273922425	20.463401688313894	40.573580771738314	23.788971266025367
3	18.22460318845244	26.37915400728121	30.40235033164843	24.99389247261792
4	12.274345657341474	17.628991041041107	37.77945850713317	32.31720479448425
5	12.942147435690291	38.019638358459176	35.67041604676083	13.367798159089705
6	30.304021296888887	37.452458550739344	18.21886734895517	14.0246528034166
7	26.687592662727706	32.150880449408575	22.50830167558771	18.65322521227601
8	25.471456971533236	35.36644675358921	21.207436543400735	17.954659731476827
9	25.756380843710836	15.691930553515682	20.32434060400597	38.22734799876751
10-11	24.651592504163375	25.991692800022747	28.47244562164157	20.884269074172305
12-13	25.527412116292414	23.274366110160333	28.374390540254073	22.823831233293177
14-15	22.733228934942527	24.794090008388924	25.460372990503323	27.012308066165225
16-17	22.632051253879393	28.18359887492745	26.057663845798878	23.12668602539427
18-19	22.550779497931206	27.03291683847376	27.812810626144753	22.603493037450285
20-21	23.123910208359355	26.224796131907226	27.39606310421781	23.255230555515606
22-23	22.983014274402773	26.668422284691484	27.376086106842152	22.972477334063594
24-25	22.929659556615142	26.85699202122565	27.594172547168007	22.6191758749912
26-27	22.76326070778481	26.98529527683985	27.67809927193895	22.573344743436394
28-29	22.152552288187227	26.57024769983761	28.202090637442943	23.07510937453222
30-31	22.71470533558835	26.457772292571352	27.397519706976908	23.43000266486339
32-33	22.978443047736096	26.65607722382773	27.941896701451718	22.423583026984456
34-35	22.71449596942931	27.266948503820316	27.78938558547892	22.22916994127145
36-37	22.508418795526104	26.856185612853906	27.46994654033019	23.165449051289798
38-39	22.57691832053753	26.562095295066886	27.895982046973376	22.965004337422208
40-41	22.5714257037182	26.4186360070192	28.28237889957828	22.72755938968432
42-43	22.925148583028815	26.37884822674715	28.033662911542322	22.662340278681715
44-45	22.438696198177222	27.10625827164278	27.649798046557567	22.805247483622434
46-47	23.142088792882777	26.41464255098719	27.03022375188932	23.413044904240714
48-49	23.063561198334373	26.314966487966206	27.190079863013842	23.43139245068558
50-51	22.463275091656996	26.6473681221762	27.57866707836895	23.310689707797856
52-53	22.615527621667724	26.99142324027994	27.69679020340414	22.6962589346482
54-55	22.6497770986623	26.97497448172761	27.10713208025392	23.268116339356165
56-57	23.211550341706204	27.27952515589223	26.57423766326909	22.934686839132475
58-59	22.469714111380885	27.67598567005075	27.43764856928572	22.41665164928264
60-61	23.07187443243373	28.29195081967369	26.112344441152118	22.523830306740464
62-63	22.6520461711841	28.54260490093915	26.18010451140681	22.62524441646994
64-65	22.53579211790703	28.10052365064636	26.45825001263612	22.9054342188105
66-67	22.8071054170412	27.6980656644925	26.449481942079604	23.045346976386693
68-69	22.87754950272699	27.421894702702858	26.68466041371918	23.015895380850974
70-71	22.983038728110117	26.8499013874738	26.76351602967319	23.403543854742892
72-73	23.010386383693067	27.02494273415959	26.795156874267295	23.16951400788005
74-75	22.840315468977526	27.238040504533128	26.786011289539697	23.135632736949653
76-77	22.94116313104352	27.106550397340467	26.68047648828431	23.2718099833317
78-79	22.96682304918124	27.071964825645207	26.699195972728106	23.262016152445444
80-81	22.892659699899333	27.097107837558	26.759348172245716	23.250884290296952
82-83	23.25365440931066	26.907576043115565	26.85342709584173	22.985342451732045
84-85	23.333809207065542	26.851508754636928	26.655608664478557	23.159073373818973
86-87	22.864970277715706	27.070334383909874	26.945795713477157	23.118899624897267
88-89	23.166539963787375	26.858083499496892	26.774906235582236	23.200470301133496
90-91	23.2231260572548	26.768186088958636	26.80507532185143	23.203612531935136
92-93	23.278196763436416	26.840442940883253	26.80698231791432	23.074377977766012
94-95	23.054098939386964	26.855765836288008	26.894432932725515	23.195702291599517
96-97	22.98993832797652	26.810242515269202	27.009892858317407	23.189926298436873
98-99	23.333310811881866	27.38175393425936	26.931385539617008	22.353549714241762
100-101	27.87772692048236	34.3998836324712	27.42529915898781	10.297090288058627
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	504.0
1	575.0
2	1323.0
3	2174.0
4	3270.5
5	5817.5
6	7758.5
7	8669.0
8	8852.5
9	8070.5
10	6882.0
11	5855.5
12	6610.0
13	9456.0
14	15577.0
15	23377.0
16	29150.0
17	32085.0
18	32160.0
19	29845.5
20	26495.5
21	23336.5
22	21919.5
23	23683.0
24	28638.0
25	36507.0
26	47226.0
27	58776.5
28	68814.5
29	80265.5
30	91186.5
31	99684.5
32	110786.5
33	123734.5
34	137122.5
35	152209.0
36	169959.0
37	184155.0
38	189981.5
39	194612.5
40	202241.0
41	207670.0
42	211553.5
43	221024.5
44	234036.0
45	248655.0
46	264003.0
47	279546.0
48	309470.5
49	323526.5
50	311254.0
51	308047.5
52	301750.5
53	286378.0
54	269128.5
55	248460.5
56	229538.5
57	212402.5
58	192497.0
59	167312.0
60	138999.5
61	111438.0
62	86402.0
63	66424.5
64	50490.0
65	37260.0
66	27400.0
67	20594.5
68	15392.0
69	11476.5
70	8510.5
71	6138.5
72	4345.0
73	3031.5
74	2134.5
75	1431.0
76	1011.5
77	715.0
78	483.0
79	310.5
80	215.0
81	167.0
82	135.5
83	98.5
84	62.0
85	32.5
86	19.0
87	13.5
88	11.5
89	9.5
90	6.5
91	4.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0015672684546668406
3	4.524073889759953E-4
4	0.0
5	0.0
6	6.301388632165648E-4
7	0.0
8	0.0
9	1.615740674914269E-5
10-11	3.7162035523028185E-4
12-13	3.877777619794245E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	5.659235443759246E-5
28-29	8.091327787699094E-6
30-31	4.05363904906168E-5
32-33	1.8705396270973527E-4
34-35	1.6330493923137428E-5
36-37	6.569602433117958E-4
38-39	1.6566084305300011E-4
40-41	5.854905243795327E-5
42-43	0.0
44-45	0.0
46-47	4.326849894913797E-5
48-49	0.0
50-51	1.153474891912748E-4
52-53	0.0
54-55	6.808457484042563E-4
56-57	3.6826735915660885E-5
58-59	0.0
60-61	9.778777470532177E-4
62-63	9.647858937496731E-6
64-65	0.0
66-67	1.9995091205109147E-5
68-69	0.0
70-71	0.0
72-73	6.282589624805842E-5
74-75	2.121455445723093E-5
76-77	1.0747499325594417E-5
78-79	4.357574264776453E-5
80-81	0.0
82-83	0.0
84-85	1.1366409509229126E-5
86-87	4.3794639559167767E-4
88-89	9.353633524170841E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	205.0
20-21	859.0
22-23	1253.0
24-25	1617.0
26-27	3800.0
28-29	9575.0
30-31	18577.0
32-33	22810.0
34-35	30758.0
36-37	48144.0
38-39	59630.0
40-41	57693.0
42-43	62934.0
44-45	74252.0
46-47	74300.0
48-49	70951.0
50-51	64836.0
52-53	61870.0
54-55	73123.0
56-57	81627.0
58-59	82551.0
60-61	84792.0
62-63	87021.0
64-65	89160.0
66-67	92684.0
68-69	74867.0
70-71	68690.0
72-73	61815.0
74-75	61284.0
76-77	61673.0
78-79	64439.0
80-81	65957.0
82-83	61528.0
84-85	60224.0
86-87	61245.0
88-89	65503.0
90-91	69310.0
92-93	68190.0
94-95	70579.0
96-97	103745.0
98-99	465397.0
100-101	3449644.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.93880096825397
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.36001085831789	72.40113343272469
2	7.472413699105163	12.24561237686865
3	2.1758084627847376	5.348494097314837
4	0.8790062577630647	2.8809887521879003
5	0.43616503801274165	1.7869420119518482
6	0.23032658277488854	1.13236104142138
7	0.13176576766728978	0.7557710307923478
8	0.08438913913774286	0.5531795900551844
9	0.05766128803023072	0.4252227123134016
>10	0.1694209740734785	1.9888422008557862
>50	0.0016512966376100412	0.08870025524260831
>100	9.956324280550569E-4	0.15546391502709175
>500	3.150026771247562E-4	0.16106027416175447
>1k	7.000059004724923E-5	0.07622830908255171
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	3.231481349828537E-5	0.0	0.0	0.0
5	0.0	3.231481349828537E-5	0.0	0.0	0.0
6	0.0	3.231481349828537E-5	0.0	0.0	0.0
7	0.0	3.231481349828537E-5	0.0	0.0	0.0
8	0.0	3.231481349828537E-5	0.0	0.0	0.0
9	0.0	3.231481349828537E-5	0.0	0.0	0.0
10-11	0.0	3.231481349828537E-5	0.0	8.078703374571343E-6	0.0
12-13	0.0	3.231481349828537E-5	0.0	3.231481349828537E-5	0.0
14-15	0.0	6.462962699657075E-5	0.0	7.270833037114209E-5	0.0
16-17	0.0	6.462962699657075E-5	0.0	9.694444049485613E-5	0.0
18-19	0.0	6.462962699657075E-5	0.0	1.3733795736771285E-4	0.0
20-21	0.0	6.462962699657075E-5	0.0	1.9388888098971225E-4	0.0
22-23	0.0	7.270833037114209E-5	0.0	3.796990586048532E-4	0.0
24-25	0.0	8.886573712028478E-5	0.0	6.947684902131356E-4	0.0
26-27	0.0	9.694444049485613E-5	0.0	8.482638543299911E-4	0.0
28-29	0.0	9.694444049485613E-5	0.0	0.001712685115409125	0.0
30-31	0.0	1.454166607422842E-4	0.0	0.003570786891560534	0.0
32-33	0.0	1.454166607422842E-4	0.0	0.006366018259162219	0.0
34-35	0.0	1.9388888098971225E-4	0.0	0.009726758862983898	0.0
36-37	0.0	2.4236110123714033E-4	0.0	0.014662846624846989	0.0
38-39	0.0	2.4236110123714033E-4	0.0	0.022886966660160615	0.0
40-41	0.0	2.58518507986283E-4	0.0	0.031781619075563664	0.0
42-43	0.0	2.746759147354257E-4	0.0	0.03987647985688415	0.0
44-45	0.0	2.9891202485913973E-4	0.0	0.048577243391297494	0.0
46-47	0.0	3.0699072823371106E-4	0.0	0.05771425690793768	0.0
48-49	0.0	3.0699072823371106E-4	0.0	0.06722289077980814	0.0
50-51	0.0	3.393055417319964E-4	0.0	0.0768527052022972	0.0
52-53	0.0	3.393055417319964E-4	0.0	0.0856746492873291	0.0
54-55	0.0	3.393055417319964E-4	0.0	0.09422999616100015	0.0
56-57	0.0	3.393055417319964E-4	0.0	0.10340740319451319	0.0
58-59	0.0	3.393055417319964E-4	0.0	0.11244747227065854	0.0
60-61	0.0	3.7162035523028185E-4	0.0	0.12126941635569044	0.0
62-63	0.0	3.877777619794245E-4	0.0	0.1293723558403855	0.0
64-65	0.0	4.524073889759953E-4	0.0	0.1368209203517403	0.0
66-67	0.0	4.8472220247428065E-4	0.0	0.14531971630178933	0.0
68-69	0.0	4.8472220247428065E-4	0.0	0.15312374376162524	0.0
70-71	0.0	4.8472220247428065E-4	0.0	0.16047536383248517	0.0
72-73	0.0	5.089583125979947E-4	0.0	0.16621932193180539	0.0
74-75	0.0	5.331944227217087E-4	0.0	0.17037985416970963	0.0
76-77	0.0	5.4127312609628E-4	0.0	0.17409605772201248	0.0
78-79	0.0	5.493518294708514E-4	0.0	0.17796575663843212	0.0
80-81	0.0	5.493518294708514E-4	0.0	0.1798884880415801	0.0
82-83	0.0	5.493518294708514E-4	0.0	0.18072867319253555	0.0
84-85	0.0	5.493518294708514E-4	0.0	0.18135073335237753	0.0
86-87	0.0	5.493518294708514E-4	0.0	0.18156077964011638	0.0
88-89	0.0	5.816666429691368E-4	0.0	0.1816092518603638	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	9775	0.0	49.157265	1
TATACAC	10995	0.0	45.15016	3
TTATACA	11825	0.0	41.11863	2
TACACAT	15545	0.0	31.802948	5
GGTATCA	11325	0.0	30.857702	1
ATACACA	16730	0.0	30.62399	4
ACACATC	16770	0.0	28.846909	6
ACATCTC	18035	0.0	26.800909	8
CACATCT	18220	0.0	26.596	7
GTATCAA	16690	0.0	26.54003	1
TACATGG	20635	0.0	24.671185	2
CAACGCA	17425	0.0	24.552881	5
ATCAACG	17510	0.0	24.387457	3
ACATGGG	20685	0.0	24.157215	3
GTACATG	21360	0.0	24.082264	1
TCAACGC	18120	0.0	23.678734	4
CATCTCC	20590	0.0	23.41572	9
AACGCAG	18550	0.0	23.217878	6
CATGGGG	15135	0.0	21.929167	4
TATCAAC	20545	0.0	21.262075	2
>>END_MODULE