FastQCFastQC Report
Fri 27 May 2016
SRR2140091_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140091_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5968940
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93300.028.8110751
GTATCAA134700.025.24031
ATCAACG138900.023.421853
CGTCGAA1802.0032554E-423.20535794-95
CAACGCA140400.023.1416025
TCAACGC142450.022.9847324
AACGCAG144850.022.6046586
GTACATG161550.022.3671111
TACATGG157300.022.0398032
ACATGGG160150.020.9676533
ACGCAGA161200.020.2078727
CGCAGAG163300.019.9480048
CATGGGG109200.019.1325594
GTACTAG18400.019.1147021
TATCAAC174750.019.072252
AGAGTAC156900.017.21245410-11
GAGTACT100150.016.88991212-13
CGACGAG7250.016.79433624-25
CTAACGC5750.016.747983
GCAGAGT198900.016.4618269
Produced by FastQC (version 0.11.5)