##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140091_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5968940
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76419112941326	34.0	31.0	34.0	31.0	34.0
2	32.9798954253184	34.0	33.0	34.0	31.0	34.0
3	33.09228740781445	34.0	33.0	34.0	31.0	34.0
4	36.463887055323056	37.0	37.0	37.0	35.0	37.0
5	36.31591739906918	37.0	37.0	37.0	35.0	37.0
6	36.34038841067258	37.0	37.0	37.0	35.0	37.0
7	36.35465928623843	37.0	37.0	37.0	35.0	37.0
8	36.370120993007134	37.0	37.0	37.0	35.0	37.0
9	38.293077497847186	39.0	39.0	39.0	37.0	39.0
10-11	38.20389172951981	39.0	39.0	39.0	37.0	39.0
12-13	38.19089159884335	39.0	39.0	39.0	37.0	39.0
14-15	39.713107938762995	41.0	40.0	41.0	38.0	41.0
16-17	39.69761364664413	41.0	40.0	41.0	38.0	41.0
18-19	39.73639800366564	41.0	40.0	41.0	38.0	41.0
20-21	39.76044880817007	41.0	40.0	41.0	38.0	41.0
22-23	39.71608972992646	41.0	40.0	41.0	38.0	41.0
24-25	39.702519032281415	41.0	40.0	41.0	37.5	41.0
26-27	39.600224240971855	41.0	40.0	41.0	37.0	41.0
28-29	39.53987646122327	41.0	39.0	41.0	37.0	41.0
30-31	39.4847577799907	40.5	39.0	41.0	37.0	41.0
32-33	39.40852263984215	40.0	39.0	41.0	36.5	41.0
34-35	39.37765499497026	40.0	39.0	41.0	36.0	41.0
36-37	39.34136641981232	40.0	39.0	41.0	36.0	41.0
38-39	39.28560360640433	40.0	39.0	41.0	36.0	41.0
40-41	39.23065837526278	40.0	39.0	41.0	36.0	41.0
42-43	39.22763865298343	40.0	39.0	41.0	36.0	41.0
44-45	39.17591545396837	40.0	39.0	41.0	35.0	41.0
46-47	39.0753466753833	40.0	39.0	41.0	35.0	41.0
48-49	39.07578408415555	40.0	39.0	41.0	35.0	41.0
50-51	38.98385830717665	40.0	39.0	41.0	35.0	41.0
52-53	38.83823361041806	40.0	38.0	41.0	35.0	41.0
54-55	38.65891070185542	40.0	38.0	41.0	35.0	41.0
56-57	38.53264601925198	40.0	38.0	41.0	35.0	41.0
58-59	38.39522859504237	40.0	37.0	41.0	34.5	41.0
60-61	38.1881186322298	40.0	37.0	41.0	34.0	41.0
62-63	38.04667165195468	39.5	36.5	41.0	34.0	41.0
64-65	37.79541179141263	39.0	36.0	41.0	34.0	41.0
66-67	37.571612915123225	39.0	35.5	41.0	34.0	41.0
68-69	37.31851729758212	38.5	35.0	40.0	34.0	41.0
70-71	36.88136993238538	37.5	35.0	40.0	33.0	41.0
72-73	36.615583619998304	37.0	35.0	39.5	33.0	41.0
74-75	36.247979234234336	36.5	35.0	39.0	33.0	41.0
76-77	34.41814492347556	34.5	33.0	36.5	30.0	39.0
78-79	35.383042920483426	35.0	34.5	37.0	32.0	39.0
80-81	35.28800774892364	35.0	35.0	37.0	32.0	39.0
82-83	34.999015746531654	35.0	34.5	37.0	32.0	39.0
84-85	34.73916348148361	35.0	34.0	36.0	32.0	37.5
86-87	34.53430421708784	35.0	34.0	36.0	32.0	37.0
88-89	34.350182280062846	35.0	34.0	36.0	32.0	37.0
90-91	34.26606962353304	35.0	34.0	35.0	32.0	36.0
92-93	34.13669949086429	35.0	34.0	35.0	32.0	36.0
94-95	33.9835629493913	35.0	34.0	35.0	31.0	36.0
96-97	34.00038401738384	35.0	34.0	35.0	31.5	36.0
98-99	34.0222817662607	35.0	34.0	35.0	32.0	35.5
100-101	32.81253570451939	34.5	32.0	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	6.0
20	22.0
21	48.0
22	203.0
23	687.0
24	1888.0
25	4304.0
26	8097.0
27	13364.0
28	20599.0
29	29711.0
30	41010.0
31	55372.0
32	76100.0
33	106904.0
34	170631.0
35	284434.0
36	596720.0
37	1388773.0
38	2097788.0
39	1049887.0
40	22389.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.58238481204368	20.191172972085496	12.848713506920827	24.37772870895
2	14.79368841764123	21.32125928740545	41.0824321526006	22.802620142352716
3	18.2539480434103	26.328643808429884	30.384680207729858	25.032727940429954
4	12.044550623728837	17.755765680338552	39.89597818038044	30.303705515552178
5	12.713011020382178	38.275254902880576	36.253254346667916	12.758479730069325
6	29.599577008410073	38.53565967538813	19.088617977846216	12.776145338355576
7	26.220266915063643	32.58580250429725	23.894912664560206	17.299017916078903
8	24.323045632892942	36.767382483321995	21.681035493739255	17.228536390045804
9	25.554529130642674	16.267516935173393	21.549629096499242	36.628324837684694
10-11	24.055566166073593	26.73274227163373	29.433967954001726	19.777723608290948
12-13	24.849968545372146	24.23425362900963	29.4721570000578	21.443620825560426
14-15	21.81636940562311	25.775648607625474	26.973323571689445	25.43465841506197
16-17	21.606700687224198	28.735177435189495	27.60291609565517	22.055205781931132
18-19	21.442308014488333	28.374041287062695	29.09744443737079	21.086206261078182
20-21	22.320326586251007	27.590828261663404	28.802072805022945	21.286772347062648
22-23	22.05211186558488	27.496429925153265	28.621108233006492	21.83034997625536
24-25	21.786688515561515	27.571332594030824	28.81288649024807	21.829092400159592
26-27	21.922730038652	27.781405177702872	28.540856701421806	21.755008082223327
28-29	21.667888181443974	28.021009327160805	28.610356303431484	21.700746187963734
30-31	22.01382721194639	27.777445410829355	28.57830784704361	21.630419530180646
32-33	21.7624428226001	27.928084908570504	28.46966243226489	21.8398098365645
34-35	21.822480950131684	28.044609255520815	28.50817845934565	21.62473133500185
36-37	21.902069509569042	27.87863561831465	28.43713743892316	21.78215743319315
38-39	21.811617116896436	27.936517208265943	28.565192158598553	21.686673516239072
40-41	21.96220922949217	27.806423643059443	28.52783464041073	21.703532487037652
42-43	21.874036136466735	27.921340456100552	28.30422332916885	21.900400078263868
44-45	21.919663750070086	28.202521011879778	28.094723095256935	21.783092142793205
46-47	21.946642999130272	28.017855696276904	27.895408242747738	22.14009306184509
48-49	22.0750763515938	28.010912025931905	27.932935547065945	21.98107607540835
50-51	21.958959605795854	28.067644205442395	27.889120665551193	22.08427552321056
52-53	22.004820849600062	28.045745221085816	27.798893824119414	22.150540105194708
54-55	22.037682145610656	28.062740758516686	27.776478621084678	22.12309847478798
56-57	22.162175552573917	28.09533867669768	27.709520083407458	22.032965687320946
58-59	22.013169853265335	28.15080806641177	27.80803054367491	22.02799153664799
60-61	22.152420877253093	28.14638224034197	27.652139195763358	22.04905768664158
62-63	22.12307112526086	28.106226230484204	27.593606143368287	22.177096500886645
64-65	22.02247395957959	28.067903578464097	27.68127874802005	22.228343713936262
66-67	22.14545841814074	28.094471439362973	27.579558325187257	22.18051181730902
68-69	22.071897078674503	28.124001069940004	27.708827426423067	22.09527442496243
70-71	22.039055780565107	27.885732254750085	27.675423147287066	22.39978881739774
72-73	22.09324986694126	28.014841728454094	27.772714910164538	22.119193494440108
74-75	22.049630574435202	28.101765334210064	27.66486380710827	22.183740284246454
76-77	22.04937447978542	28.00371239854829	27.590571265319696	22.356341856346592
78-79	22.10411425933522	27.953238449545754	27.631852381189248	22.31079490992978
80-81	21.997399266344107	28.083823762480282	27.645380159054078	22.273396812121536
82-83	22.18370052277003	27.94361455011916	27.79112720620801	22.0815577209028
84-85	22.406764269445997	27.907392117434078	27.626783296886387	22.059060316233538
86-87	22.033824807286447	28.069222809306726	27.660673997410502	22.236278385996325
88-89	22.227217073261293	27.916460908200175	27.647102305740066	22.20921971279846
90-91	22.31222419781293	27.954273264116004	27.621963297004985	22.111539241066083
92-93	22.298200493106847	27.926677968736396	27.623614196194346	22.15150734196241
94-95	22.07910018819252	28.024061588553717	27.716723978764858	22.1801142444889
96-97	22.070832878654294	27.98349925497017	27.813694739596862	22.131973126778682
98-99	22.33819698320599	28.532630411794813	27.82408890801737	21.30508369698183
100-101	26.35812575633956	35.58256143884975	28.41334011468821	9.645972690122468
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	568.0
1	590.5
2	1136.0
3	1784.5
4	2709.0
5	4795.5
6	6392.5
7	7145.5
8	7208.0
9	6481.0
10	5524.0
11	4828.0
12	5394.0
13	7565.0
14	12375.5
15	18455.0
16	22975.5
17	25519.5
18	26066.5
19	25010.5
20	23602.5
21	22703.0
22	23472.0
23	27097.0
24	33283.5
25	42389.0
26	54581.0
27	67908.0
28	81472.0
29	95741.5
30	108218.5
31	119829.0
32	133409.5
33	146751.5
34	161244.0
35	176928.0
36	192237.5
37	206538.0
38	215981.5
39	222592.0
40	231745.5
41	239167.0
42	244352.0
43	252559.5
44	260933.5
45	268249.0
46	273393.5
47	275243.0
48	272313.0
49	265784.0
50	258720.0
51	248732.0
52	234403.0
53	220029.0
54	204349.0
55	185488.0
56	167674.0
57	148203.0
58	127710.0
59	109217.0
60	90853.0
61	73053.5
62	57693.0
63	44525.5
64	33051.5
65	24109.5
66	17856.5
67	13441.5
68	10071.5
69	7241.0
70	5283.0
71	3913.5
72	2763.0
73	1910.5
74	1293.5
75	849.0
76	581.5
77	389.5
78	250.5
79	167.5
80	112.0
81	79.0
82	57.5
83	40.5
84	27.5
85	18.0
86	15.0
87	14.0
88	11.0
89	9.5
90	8.5
91	10.0
92	12.5
93	10.5
94	8.5
95	6.0
96	3.0
97	1.5
98	1.5
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0017423529135826461
3	5.696153755943266E-4
4	0.0
5	0.0
6	4.523416217954947E-4
7	0.0
8	0.0
9	3.350678679966627E-5
10-11	3.3506786799666275E-4
12-13	4.1883483499582843E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	1.0059283549324314E-4
28-29	3.355424610110146E-5
30-31	5.881771189521843E-5
32-33	3.201848309065991E-4
34-35	5.073535828295312E-5
36-37	8.753870400563511E-4
38-39	2.5694395315808955E-4
40-41	9.503495083107632E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	9.00213251517152E-6
50-51	6.369986661247932E-5
52-53	0.0
54-55	7.240979711981677E-4
56-57	8.445082100273152E-5
58-59	0.0
60-61	8.925432617158539E-4
62-63	0.0
64-65	0.0
66-67	7.932870463563235E-5
68-69	0.0
70-71	0.0
72-73	1.2334514500146883E-4
74-75	3.119313102300368E-5
76-77	4.2090668772322526E-5
78-79	8.52114922609325E-5
80-81	0.0
82-83	0.0
84-85	2.2132646483537463E-5
86-87	5.154315153428835E-4
88-89	1.2490266108525878E-4
90-91	2.3031492571998174E-5
92-93	0.0
94-95	1.1865857431011014E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	215.0
20-21	765.0
22-23	1183.0
24-25	1607.0
26-27	3184.0
28-29	7648.0
30-31	15770.0
32-33	19650.0
34-35	26219.0
36-37	41955.0
38-39	51332.0
40-41	49615.0
42-43	54155.0
44-45	62577.0
46-47	63345.0
48-49	60898.0
50-51	55569.0
52-53	53332.0
54-55	56901.0
56-57	59223.0
58-59	58972.0
60-61	57770.0
62-63	54676.0
64-65	55662.0
66-67	58522.0
68-69	60351.0
70-71	59482.0
72-73	55720.0
74-75	57044.0
76-77	56693.0
78-79	59321.0
80-81	60777.0
82-83	56797.0
84-85	55556.0
86-87	58143.0
88-89	60584.0
90-91	63877.0
92-93	64030.0
94-95	67378.0
96-97	104483.0
98-99	470198.0
100-101	3587761.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.46826679660151
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.84594455168624	75.046629481879
2	7.6851883776827306	12.98309084536492
3	2.0306409293213457	5.1457415935804285
4	0.7281906428764209	2.4603600600509714
5	0.3127404111142648	1.320832024203928
6	0.15639986279682702	0.7926495202604553
7	0.08491451003924402	0.5020807042228292
8	0.043324777588356976	0.29276550977894067
9	0.029470018204396303	0.2240353224170685
>10	0.08106343346980197	0.9619376828531294
>50	0.0013085361442098358	0.07294106444352833
>100	6.915057824841248E-4	0.12275409865780797
>500	9.183248667791718E-5	0.046774289118656005
>1k	3.061080695024322E-5	0.027407803168323527
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.6753393399833136E-5	0.0	0.0	0.0
5	0.0	1.6753393399833136E-5	0.0	0.0	0.0
6	6.701357359933255E-5	1.6753393399833136E-5	0.0	0.0	0.0
7	6.701357359933255E-5	1.6753393399833136E-5	0.0	0.0	0.0
8	6.701357359933255E-5	3.350678679966627E-5	0.0	1.6753393399833136E-5	0.0
9	6.701357359933255E-5	6.701357359933255E-5	0.0	1.6753393399833136E-5	3.350678679966627E-5
10-11	6.701357359933255E-5	6.701357359933255E-5	0.0	1.6753393399833136E-5	3.350678679966627E-5
12-13	6.701357359933255E-5	6.701357359933255E-5	0.0	5.026018019949941E-5	3.350678679966627E-5
14-15	6.701357359933255E-5	1.256504504987485E-4	0.0	1.0889705709891538E-4	5.863687689941598E-5
16-17	1.340271471986651E-4	1.340271471986651E-4	0.0	1.9266402409808106E-4	6.701357359933255E-5
18-19	1.340271471986651E-4	1.5078054059849822E-4	0.0	2.7643099109724673E-4	1.0889705709891538E-4
20-21	1.5078054059849822E-4	1.6753393399833135E-4	0.0	3.9370474489607867E-4	1.1727375379883195E-4
22-23	1.591572372984148E-4	1.9266402409808106E-4	0.0	6.114988590939095E-4	1.340271471986651E-4
24-25	1.6753393399833135E-4	2.0941741749791421E-4	0.0	8.795531534912396E-4	1.5078054059849822E-4
26-27	1.6753393399833135E-4	2.1779411419783077E-4	0.0	0.0011141006610889035	1.5078054059849822E-4
28-29	1.6753393399833135E-4	2.1779411419783077E-4	0.0	0.0017926130937821457	1.5078054059849822E-4
30-31	1.7591063069824793E-4	2.1779411419783077E-4	0.0	0.003601979580964124	1.5078054059849822E-4
32-33	1.842873273981645E-4	2.1779411419783077E-4	0.0	0.0063746661886365085	1.6753393399833135E-4
34-35	1.842873273981645E-4	2.345475075976639E-4	0.0	0.010479247571595627	1.6753393399833135E-4
36-37	1.842873273981645E-4	2.51300900997497E-4	0.0	0.01655235267903514	1.6753393399833135E-4
38-39	1.842873273981645E-4	2.51300900997497E-4	0.0	0.02577509574564328	1.6753393399833135E-4
40-41	1.842873273981645E-4	2.51300900997497E-4	0.0	0.036120316170040245	1.6753393399833135E-4
42-43	1.842873273981645E-4	2.51300900997497E-4	0.0	0.045895921218842874	1.842873273981645E-4
44-45	1.842873273981645E-4	2.51300900997497E-4	0.0	0.056408675577238165	2.0104072079799764E-4
46-47	1.842873273981645E-4	2.51300900997497E-4	0.0	0.06801877720332253	2.0104072079799764E-4
48-49	1.842873273981645E-4	2.51300900997497E-4	0.0	0.07989693312380422	2.1779411419783077E-4
50-51	1.842873273981645E-4	2.51300900997497E-4	0.0	0.0912557338488911	2.1779411419783077E-4
52-53	1.842873273981645E-4	2.51300900997497E-4	0.0	0.10228784340268121	2.345475075976639E-4
54-55	1.842873273981645E-4	2.51300900997497E-4	0.0	0.11398171199576473	2.51300900997497E-4
56-57	1.842873273981645E-4	2.51300900997497E-4	0.0	0.12259295620327898	2.51300900997497E-4
58-59	1.842873273981645E-4	2.51300900997497E-4	0.0	0.13026601038040253	2.51300900997497E-4
60-61	2.0104072079799764E-4	2.51300900997497E-4	0.0	0.13748672293573064	2.51300900997497E-4
62-63	2.345475075976639E-4	2.51300900997497E-4	0.0	0.1443974977131618	2.680542943973302E-4
64-65	2.345475075976639E-4	2.8480768779716334E-4	0.0	0.15129989579389305	2.680542943973302E-4
66-67	2.345475075976639E-4	3.0156108119699644E-4	0.0	0.15812690360432505	2.680542943973302E-4
68-69	2.345475075976639E-4	3.4344456469657925E-4	0.0	0.16531410937285346	2.680542943973302E-4
70-71	2.345475075976639E-4	3.5182126139649586E-4	0.0	0.17147935814399207	2.8480768779716334E-4
72-73	2.7643099109724673E-4	3.5182126139649586E-4	0.0	0.1768571974253385	3.853280481961621E-4
74-75	2.8480768779716334E-4	3.5182126139649586E-4	0.0	0.18147275730699253	3.853280481961621E-4
76-77	2.931843844970799E-4	3.68574654796329E-4	0.0	0.1854600649361528	3.853280481961621E-4
78-79	3.0156108119699644E-4	3.853280481961621E-4	0.0	0.1890452911237171	3.853280481961621E-4
80-81	3.0156108119699644E-4	3.853280481961621E-4	0.0	0.19083790421749924	4.104581382959118E-4
82-83	3.0156108119699644E-4	3.853280481961621E-4	0.0	0.19144102637989324	4.3558822839566153E-4
84-85	3.0156108119699644E-4	3.853280481961621E-4	0.0	0.19195200487858816	4.3558822839566153E-4
86-87	3.0156108119699644E-4	3.853280481961621E-4	0.0	0.19211953881258648	4.3558822839566153E-4
88-89	3.0156108119699644E-4	3.853280481961621E-4	0.0	0.19216142229608607	4.3558822839566153E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9330	0.0	28.811075	1
GTATCAA	13470	0.0	25.2403	1
ATCAACG	13890	0.0	23.42185	3
CGTCGAA	180	2.0032554E-4	23.205357	94-95
CAACGCA	14040	0.0	23.141602	5
TCAACGC	14245	0.0	22.984732	4
AACGCAG	14485	0.0	22.604658	6
GTACATG	16155	0.0	22.367111	1
TACATGG	15730	0.0	22.039803	2
ACATGGG	16015	0.0	20.967653	3
ACGCAGA	16120	0.0	20.207872	7
CGCAGAG	16330	0.0	19.948004	8
CATGGGG	10920	0.0	19.132559	4
GTACTAG	1840	0.0	19.114702	1
TATCAAC	17475	0.0	19.07225	2
AGAGTAC	15690	0.0	17.212454	10-11
GAGTACT	10015	0.0	16.889912	12-13
CGACGAG	725	0.0	16.794336	24-25
CTAACGC	575	0.0	16.74798	3
GCAGAGT	19890	0.0	16.461826	9
>>END_MODULE