FastQCFastQC Report
Fri 27 May 2016
SRR2140090_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140090_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5265588
Sequences flagged as poor quality0
Sequence length19-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92550.032.8708761
GTATCAA136400.027.5146181
ATCAACG151700.023.8020213
CAACGCA154200.023.2576455
ACGGTAC4150.022.5100983
AACGCAG163200.022.4479036
TACATGG183450.022.2733822
TATCAAC167500.022.1634462
TCAACGC163250.022.0678394
GTACATG191200.021.6892091
ACATGGG181100.021.5527463
CGCAGAG179550.020.1095168
ACGCAGA180000.020.0818047
CATGGGG134350.019.529194
GGTTCAC21200.018.0086616
TAACGCC7800.017.7040944
TCTAACG8450.017.3042072
CTAACGC8250.017.231053
ACGGGCG13050.016.806325
GAGTACT105400.016.74304412-13
Produced by FastQC (version 0.11.5)