##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140090_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5265588
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79619882907664	34.0	31.0	34.0	31.0	34.0
2	32.99706452536735	34.0	33.0	34.0	31.0	34.0
3	33.105216549414806	34.0	33.0	34.0	31.0	34.0
4	36.468347314677864	37.0	37.0	37.0	35.0	37.0
5	36.331956848883735	37.0	37.0	37.0	35.0	37.0
6	36.366492023303	37.0	37.0	37.0	35.0	37.0
7	36.37200707689246	37.0	37.0	37.0	35.0	37.0
8	36.38676288384127	37.0	37.0	37.0	35.0	37.0
9	38.29682307085173	39.0	39.0	39.0	37.0	39.0
10-11	38.206691066600726	39.0	39.0	39.0	37.0	39.0
12-13	38.1885653606017	39.0	39.0	39.0	37.0	39.0
14-15	39.69770232688163	41.0	40.0	41.0	37.5	41.0
16-17	39.6771163068588	41.0	40.0	41.0	37.5	41.0
18-19	39.7147781976106	41.0	40.0	41.0	38.0	41.0
20-21	39.7342831374268	41.0	40.0	41.0	38.0	41.0
22-23	39.68528887338681	41.0	40.0	41.0	37.5	41.0
24-25	39.65732795618932	41.0	40.0	41.0	37.0	41.0
26-27	39.54404056111607	41.0	39.0	41.0	37.0	41.0
28-29	39.48090118310316	41.0	39.0	41.0	37.0	41.0
30-31	39.41117761004191	40.0	39.0	41.0	36.5	41.0
32-33	39.32706442946002	40.0	39.0	41.0	36.0	41.0
34-35	39.2918104930024	40.0	39.0	41.0	36.0	41.0
36-37	39.2559706061099	40.0	39.0	41.0	35.5	41.0
38-39	39.190051547608775	40.0	39.0	41.0	35.0	41.0
40-41	39.12428176910194	40.0	39.0	41.0	35.0	41.0
42-43	39.10703082004521	40.0	39.0	41.0	35.0	41.0
44-45	39.035579410226845	40.0	39.0	41.0	35.0	41.0
46-47	38.917375049300034	40.0	38.0	41.0	35.0	41.0
48-49	38.89649783816803	40.0	38.0	41.0	35.0	41.0
50-51	38.77463570300729	40.0	38.0	41.0	35.0	41.0
52-53	38.59864323440459	40.0	38.0	41.0	35.0	41.0
54-55	38.38591403283428	40.0	37.0	41.0	34.5	41.0
56-57	38.218581699480396	40.0	37.0	41.0	34.0	41.0
58-59	38.04165311872583	39.5	36.5	41.0	34.0	41.0
60-61	37.785625626056714	39.0	36.0	41.0	34.0	41.0
62-63	37.600147323537314	39.0	35.0	41.0	34.0	41.0
64-65	37.317024581418195	38.5	35.0	40.0	33.5	41.0
66-67	37.0709866267277	37.5	35.0	40.0	33.0	41.0
68-69	36.801588095291606	37.0	35.0	40.0	33.0	41.0
70-71	36.36154889877515	36.5	35.0	39.0	33.0	41.0
72-73	36.1054040714739	36.0	35.0	39.0	33.0	41.0
74-75	35.757172861439706	35.5	35.0	38.5	32.5	40.0
76-77	33.995090450647524	34.5	33.0	36.5	30.0	39.0
78-79	35.00181559369098	35.0	34.0	37.0	32.0	39.0
80-81	34.952441252681425	35.0	34.0	36.5	32.0	39.0
82-83	34.70421611057361	35.0	34.0	36.0	32.0	37.5
84-85	34.48558586271023	35.0	34.0	36.0	32.0	37.0
86-87	34.32475559800825	35.0	34.0	35.5	32.0	37.0
88-89	34.183037467884745	35.0	34.0	35.0	32.0	36.0
90-91	34.129627499495044	35.0	34.0	35.0	32.0	36.0
92-93	34.0204952159865	35.0	34.0	35.0	31.5	36.0
94-95	33.88265925872985	35.0	34.0	35.0	31.0	36.0
96-97	33.91569056265093	35.0	34.0	35.0	31.0	35.0
98-99	33.94434463334865	35.0	34.0	35.0	31.0	35.0
100-101	32.6838528922393	34.0	31.5	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	7.0
20	17.0
21	67.0
22	208.0
23	653.0
24	1707.0
25	3899.0
26	7325.0
27	12024.0
28	18560.0
29	26488.0
30	36063.0
31	49185.0
32	68442.0
33	99288.0
34	167281.0
35	286421.0
36	614841.0
37	1325722.0
38	1668612.0
39	857055.0
40	21722.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.45178544162589	19.65624731748857	12.468351112924141	24.4236161279614
2	15.57963390343474	20.79181144568337	39.96048995194375	23.668064698938142
3	18.7103484870181	26.045273040180707	29.740472147132635	25.503906325668563
4	12.581690781732258	17.137649204609247	38.52075019921802	31.75990981444048
5	13.37018771692734	37.993002870714534	35.19768352556258	13.43912588679555
6	30.768980959834607	37.284270047229604	18.48726999804199	13.459478994893795
7	27.13824173102795	32.209090418771844	23.040389791225596	17.61227805897461
8	25.672137660599347	35.30397744753292	20.96377460598892	18.060110285878807
9	26.509712972171958	15.503057873699552	20.916851245644597	37.0703779084839
10-11	25.204144546160762	25.951822314410183	28.257336978015964	20.58669616141309
12-13	25.83984718846256	23.63714210671549	28.356943624917413	22.166067079904533
14-15	22.902731850649918	24.86773556913302	26.077657424014184	26.15187515620288
16-17	22.43035345720174	27.63717936154519	26.81980625905407	23.112660922199
18-19	22.168578324016234	27.36029670380592	28.42170712938422	22.049417842793627
20-21	23.310233285226435	26.54037513505732	27.964466606917632	22.184924972798616
22-23	23.103373085442662	26.56815252410636	27.707950206598774	22.620524183852208
24-25	22.739067275694886	26.56021686252819	28.14074938891528	22.559966472861646
26-27	22.82800986302932	26.771492234527255	27.756056308585492	22.644441593857938
28-29	22.437406027014767	27.27485237741548	27.692199421968066	22.595542173601686
30-31	22.876661107679304	26.884803148082785	27.638578578019484	22.599957166218424
32-33	22.585277733687068	27.039495167259425	27.536790548967144	22.83843655008636
34-35	22.772629677570684	27.10779735706111	27.677125596074383	22.442447369293824
36-37	22.890574415842966	26.89023180458383	27.598682204829274	22.620511574743936
38-39	22.65222827749179	26.92091302548715	27.753559306919772	22.67329939010129
40-41	22.83611401765212	26.713581502806004	27.821621539279057	22.628682940262816
42-43	22.863839895667308	26.902018904396684	27.391633713331043	22.84250748660496
44-45	22.81160989003247	27.3783697784988	27.097660284543252	22.712360046925482
46-47	22.897023772291668	27.083758563588656	26.902013780752377	23.117203883367296
48-49	23.101413457433985	27.07353882024366	26.833419089137678	22.99162863318468
50-51	22.91195886617857	27.053743901156242	26.86256407846902	23.17173315419617
52-53	22.953758949656837	27.14919579966488	26.584072780770747	23.312972469907535
54-55	23.033568836324626	27.152175772381938	26.566037727657353	23.248217663636083
56-57	23.162829961283133	27.167741841803387	26.480032367457767	23.189395829455705
58-59	22.98967188275002	27.261340926043147	26.722020383132566	23.026966808074267
60-61	23.145776245982177	27.263589490847302	26.60304223748361	22.98759202568692
62-63	23.17718936732125	27.0756463172402	26.42819932223887	23.31896499319968
64-65	22.969836238725318	27.212399665652903	26.497762961296257	23.320001134325526
66-67	23.08286889205717	27.127219597261664	26.401253696874004	23.38865781380716
68-69	23.023215389831382	27.069064206706294	26.57207659338016	23.335643810082157
70-71	23.079276332115832	26.837750652832288	26.508212781278523	23.574760233773357
72-73	23.097996958184268	26.99801112048423	26.733200741365614	23.17079117996589
74-75	23.065005426589916	27.14532645091028	26.631405280715185	23.15826284178462
76-77	23.083774008536228	27.063946950455797	26.428994538309443	23.423284502698536
78-79	22.969719703661486	27.0960371391459	26.455211726489086	23.479031430703525
80-81	22.91799976463025	27.086199534772486	26.67396286774401	23.321837832853255
82-83	23.28749129963718	26.91574493017287	26.81019954512659	22.98656422506336
84-85	23.457236029215206	26.98858069768241	26.429352030850094	23.124831242252295
86-87	22.94028701932739	27.18245564625832	26.645343694502284	23.231913639912012
88-89	23.267899393616545	26.907824882301952	26.505397682061542	23.31887804201996
90-91	23.26163225641925	26.94510598988803	26.547698626724852	23.24556312696787
92-93	23.402236139504115	26.968648183898164	26.513365514094268	23.115750162503456
94-95	22.932784113844814	26.975728851582225	26.7921261060501	23.29936092852286
96-97	23.09195302901991	27.058215746848123	26.66014623266022	23.189684991471747
98-99	23.30394335951711	27.697282390574152	26.630844129299337	22.3679301206094
100-101	27.75023431966716	34.4683271100534	27.27812133891298	10.503317231366461
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	582.0
1	657.5
2	1376.0
3	2157.0
4	3221.5
5	5743.0
6	7657.5
7	8507.5
8	8429.0
9	7341.0
10	6060.5
11	5065.5
12	5728.0
13	8506.5
14	14211.0
15	21368.5
16	26573.0
17	29079.5
18	28985.5
19	26815.0
20	23807.5
21	20843.0
22	19324.5
23	20300.5
24	23479.0
25	28776.5
26	36510.0
27	44877.5
28	53641.5
29	64882.5
30	75798.0
31	86066.0
32	98300.5
33	110769.0
34	122737.0
35	135106.5
36	146776.0
37	160210.5
38	169729.5
39	173120.5
40	177325.0
41	181139.0
42	185596.5
43	196654.5
44	211876.0
45	228380.0
46	241708.5
47	249807.0
48	252632.5
49	250783.5
50	246547.0
51	244488.0
52	243391.0
53	240194.0
54	236948.5
55	225950.0
56	209791.0
57	187300.0
58	162367.0
59	139537.0
60	113590.5
61	90934.0
62	73298.0
63	57394.5
64	43235.0
65	31556.5
66	23066.0
67	17759.0
68	13766.5
69	10400.5
70	7761.5
71	5195.5
72	3685.5
73	2608.5
74	1707.0
75	1094.0
76	727.5
77	471.5
78	296.5
79	201.0
80	160.5
81	127.5
82	94.0
83	68.0
84	44.5
85	26.0
86	18.0
87	14.5
88	10.5
89	6.0
90	4.5
91	3.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001747193285916027
3	4.937720155849642E-4
4	0.0
5	0.0
6	6.646930979028364E-4
7	0.0
8	0.0
9	1.8991231368652466E-5
10-11	3.323465489514182E-4
12-13	3.2285093326709193E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	3.8007805663049015E-5
28-29	0.0
30-31	3.812529171805241E-5
32-33	1.5308143846417985E-4
34-35	4.806564613686789E-5
36-37	6.87153453270323E-4
38-39	1.8577744585540786E-4
40-41	2.967539568430721E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	1.0436810798675903E-5
50-51	8.46583242942935E-5
52-53	0.0
54-55	7.05505427887414E-4
56-57	1.1000347390970606E-4
58-59	1.1156123590660851E-5
60-61	8.825019881751521E-4
62-63	0.0
64-65	0.0
66-67	5.8914292077029255E-5
68-69	0.0
70-71	0.0
72-73	1.231502827530492E-4
74-75	0.0
76-77	2.5341325480563393E-5
78-79	9.001613089065561E-5
80-81	0.0
82-83	0.0
84-85	0.0
86-87	2.871005320793147E-4
88-89	1.1109472463922683E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	114.0
20-21	483.0
22-23	911.0
24-25	1438.0
26-27	3435.0
28-29	8829.0
30-31	18955.0
32-33	23317.0
34-35	30748.0
36-37	48482.0
38-39	60302.0
40-41	56980.0
42-43	61800.0
44-45	71292.0
46-47	70529.0
48-49	67348.0
50-51	61333.0
52-53	57621.0
54-55	60819.0
56-57	63514.0
58-59	62117.0
60-61	61164.0
62-63	57560.0
64-65	58025.0
66-67	60950.0
68-69	61628.0
70-71	61654.0
72-73	57056.0
74-75	56649.0
76-77	57359.0
78-79	60066.0
80-81	60353.0
82-83	56397.0
84-85	55096.0
86-87	56232.0
88-89	58808.0
90-91	61176.0
92-93	60137.0
94-95	62960.0
96-97	91844.0
98-99	391401.0
100-101	2888706.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.59821455757861
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.28023625936086	72.03509659485059
2	7.566853177376457	12.34883418186519
3	2.1717692649035136	5.3163748334145495
4	0.8603230336877216	2.8080329396671053
5	0.4145725078753086	1.691418822364144
6	0.23646264439418874	1.1576957755277655
7	0.13331049495839906	0.7614528859274713
8	0.08723748684940925	0.5694738535521631
9	0.055675301097835375	0.4088704648085067
>10	0.18779604637408048	2.39133703449646
>50	0.004550554936823152	0.24193882086702417
>100	0.001040891344521461	0.1845018704814931
>500	1.723368408476899E-4	0.08497192217756902
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.798246273730493E-5	0.0	0.0	0.0
3	0.0	3.798246273730493E-5	0.0	0.0	0.0
4	0.0	9.495615684326233E-5	0.0	0.0	0.0
5	1.5192985094921973E-4	9.495615684326233E-5	0.0	0.0	0.0
6	2.0890354505517713E-4	9.495615684326233E-5	0.0	0.0	0.0
7	3.0385970189843945E-4	1.3293861958056725E-4	0.0	0.0	0.0
8	3.6083339600439683E-4	1.3293861958056725E-4	0.0	0.0	0.0
9	3.6083339600439683E-4	1.3293861958056725E-4	0.0	0.0	0.0
10-11	3.6083339600439683E-4	1.3293861958056725E-4	0.0	0.0	0.0
12-13	4.3679832147900673E-4	1.8991231368652465E-4	0.0	1.8991231368652466E-5	0.0
14-15	6.362062508498577E-4	2.0890354505517713E-4	0.0	1.8991231368652466E-5	0.0
16-17	6.931799449558149E-4	2.2789477642382958E-4	0.0	3.798246273730493E-5	0.0
18-19	9.780484154856021E-4	2.468860077924821E-4	0.0	7.596492547460986E-5	0.0
20-21	0.0012534212703310627	2.6587723916113456E-4	0.0	1.3293861958056728E-4	0.0
22-23	0.0013388818114899988	3.133553175827657E-4	0.0	2.1839916073950334E-4	0.0
24-25	0.0017661845172846794	4.2730270579468047E-4	0.0	2.84868470529787E-4	0.0
26-27	0.0018041669800219842	4.7478078421631163E-4	0.0	3.513377803200706E-4	0.0
28-29	0.0022314696858166646	4.7478078421631163E-4	0.0	8.451097959050347E-4	0.0
30-31	0.0023739039210815584	5.127632469536166E-4	0.0	0.0025448250033994306	0.0
32-33	0.0024403732308718416	5.127632469536166E-4	0.0	0.005194101779326449	0.0
34-35	0.0026207899288740403	5.69736941059574E-4	0.0	0.008840418202107722	0.0
36-37	0.0029151540150881535	6.551974822185101E-4	0.0	0.01381612082069467	0.0
38-39	0.0034564041090947486	6.741887135871625E-4	0.0	0.021735464301422747	0.0
40-41	0.0034753953404634014	6.93179944955815E-4	0.0	0.030832264127007278	0.0
42-43	0.003703290116887231	7.121711763244674E-4	0.0	0.03878959007047266	0.0
44-45	0.004026141050154323	7.216667920087937E-4	0.0	0.0477439556607923	0.0
46-47	0.004529408681423613	7.216667920087937E-4	0.0	0.05726805819217151	0.0
48-49	0.004766799073531769	7.596492547460986E-4	0.0	0.06673518702944475	0.0
50-51	0.004766799073531769	8.071273331677298E-4	0.0	0.07605988163145314	0.0
52-53	0.004776294689216095	8.261185645363823E-4	0.0	0.08461543136303107	0.0
54-55	0.004861755230375031	8.356141802207085E-4	0.0	0.09346534518082311	0.0
56-57	0.005089650006798861	8.735966429580135E-4	0.0	0.10247668446524871	0.0
58-59	0.005127632469536166	8.830922586423397E-4	0.0	0.11163995360062352	0.0
60-61	0.005137128085220492	9.115791056953183E-4	0.0	0.12044238933999393	0.0
62-63	0.005241579857748081	9.115791056953183E-4	0.0	0.12852315828735555	0.0
64-65	0.00531754478322269	9.210747213796446E-4	0.0	0.1361481376818695	0.0
66-67	0.005526448328277867	9.685527998012758E-4	0.0	0.1436021959940656	0.0
68-69	0.00562140448512113	9.685527998012758E-4	0.0	0.1513791052395288	0.0
70-71	0.005754343104701697	9.875440311699283E-4	0.0	0.15877618985761893	0.0
72-73	0.005868290492913612	0.0010065352625385807	0.0	0.16482489704853476	0.0
74-75	0.0059252641870195695	0.0010065352625385807	0.0	0.16992404267101796	0.0
76-77	0.005963246649756874	0.001016030878222907	0.0	0.17444395573675722	0.0
78-79	0.005991733496809853	0.0010445177252758856	0.0	0.17890689510839053	0.0
80-81	0.006466514281026165	0.001063508956644538	0.0	0.1807680357825185	0.0
82-83	0.00662793974765971	0.001063508956644538	0.0	0.18166062365684515	0.0
84-85	0.006741887135871625	0.001063508956644538	0.0	0.18223985621358907	0.0
86-87	0.006893816986820845	0.001063508956644538	0.0	0.18248674222138156	0.0
88-89	0.0069412950652424765	0.001063508956644538	0.0	0.1825057334527502	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9255	0.0	32.870876	1
GTATCAA	13640	0.0	27.514618	1
ATCAACG	15170	0.0	23.802021	3
CAACGCA	15420	0.0	23.257645	5
ACGGTAC	415	0.0	22.510098	3
AACGCAG	16320	0.0	22.447903	6
TACATGG	18345	0.0	22.273382	2
TATCAAC	16750	0.0	22.163446	2
TCAACGC	16325	0.0	22.067839	4
GTACATG	19120	0.0	21.689209	1
ACATGGG	18110	0.0	21.552746	3
CGCAGAG	17955	0.0	20.109516	8
ACGCAGA	18000	0.0	20.081804	7
CATGGGG	13435	0.0	19.52919	4
GGTTCAC	2120	0.0	18.008661	6
TAACGCC	780	0.0	17.704094	4
TCTAACG	845	0.0	17.304207	2
CTAACGC	825	0.0	17.23105	3
ACGGGCG	1305	0.0	16.80632	5
GAGTACT	10540	0.0	16.743044	12-13
>>END_MODULE