FastQCFastQC Report
Fri 27 May 2016
SRR2140089_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140089_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5911083
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69050.042.726571
GTATCAA120500.033.9399951
ATCAACG142050.027.7698633
CAACGCA144650.027.24235
TATCAAC147650.027.109742
TCAACGC147200.026.9674194
AACGCAG152550.025.9132276
GTACATG200400.023.6956481
TACATGG199100.023.6422482
ACGCAGA168100.023.3189377
ACATGGG198800.023.1358053
CGCAGAG177150.022.0574828
CATGGGG148850.020.4328484
GTACTAG18300.019.4728281
GCAGAGT209650.018.6381249
ACGGTAC6950.017.8860513
GTACTGG35050.017.8513321
GAGTACT105150.017.85125512-13
AGAGTAC185200.016.9930110-11
TAACGCC6700.016.6982384
Produced by FastQC (version 0.11.5)