##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140089_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5911083
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.80541399943124	34.0	31.0	34.0	31.0	34.0
2	33.00343642611684	34.0	33.0	34.0	31.0	34.0
3	33.108430553250564	34.0	33.0	34.0	31.0	34.0
4	36.471991342364845	37.0	37.0	37.0	35.0	37.0
5	36.34000199286662	37.0	37.0	37.0	35.0	37.0
6	36.37320200037794	37.0	37.0	37.0	35.0	37.0
7	36.37672149079957	37.0	37.0	37.0	35.0	37.0
8	36.39046042831745	37.0	37.0	37.0	35.0	37.0
9	38.29522170471976	39.0	39.0	39.0	37.0	39.0
10-11	38.20838482558949	39.0	39.0	39.0	37.0	39.0
12-13	38.18781575220649	39.0	39.0	39.0	37.0	39.0
14-15	39.7084745722569	41.0	40.0	41.0	38.0	41.0
16-17	39.68824545349811	41.0	40.0	41.0	37.5	41.0
18-19	39.72411637596697	41.0	40.0	41.0	38.0	41.0
20-21	39.73860147402951	41.0	40.0	41.0	38.0	41.0
22-23	39.691609792389286	41.0	40.0	41.0	37.5	41.0
24-25	39.66895219747667	41.0	40.0	41.0	37.0	41.0
26-27	39.55552962835609	41.0	39.5	41.0	37.0	41.0
28-29	39.49431686954629	41.0	39.0	41.0	37.0	41.0
30-31	39.42958328871383	40.5	39.0	41.0	37.0	41.0
32-33	39.34584990567559	40.0	39.0	41.0	36.0	41.0
34-35	39.31189693172493	40.0	39.0	41.0	36.0	41.0
36-37	39.27253841755687	40.0	39.0	41.0	36.0	41.0
38-39	39.20945903980744	40.0	39.0	41.0	35.0	41.0
40-41	39.144444684071274	40.0	39.0	41.0	35.0	41.0
42-43	39.131545192192874	40.0	39.0	41.0	35.0	41.0
44-45	39.06542920181127	40.0	39.0	41.0	35.0	41.0
46-47	38.95030064442939	40.0	38.0	41.0	35.0	41.0
48-49	38.93149164144826	40.0	38.0	41.0	35.0	41.0
50-51	38.81302383879927	40.0	38.0	41.0	35.0	41.0
52-53	38.64335451691157	40.0	38.0	41.0	35.0	41.0
54-55	38.431446900592334	40.0	37.5	41.0	34.5	41.0
56-57	38.273121506391504	40.0	37.0	41.0	34.0	41.0
58-59	38.098924846458274	39.5	36.5	41.0	34.0	41.0
60-61	37.85698857411977	39.0	36.0	41.0	34.0	41.0
62-63	37.67059821895105	39.0	35.5	41.0	34.0	41.0
64-65	37.38118260606084	39.0	35.0	40.5	33.5	41.0
66-67	37.12615442281298	38.0	35.0	40.0	33.0	41.0
68-69	36.85038294817135	37.0	35.0	40.0	33.0	41.0
70-71	36.405718869060706	37.0	35.0	39.0	33.0	41.0
72-73	36.13811302274071	36.0	35.0	39.0	33.0	41.0
74-75	35.779049323266165	36.0	35.0	39.0	32.5	40.0
76-77	34.017131153212986	34.5	33.0	36.5	30.0	39.0
78-79	35.023759067360004	35.0	34.0	37.0	32.0	39.0
80-81	34.96777501553409	35.0	34.0	36.5	32.0	39.0
82-83	34.7214652869169	35.0	34.0	36.0	32.0	37.5
84-85	34.498896986449395	35.0	34.0	36.0	32.0	37.0
86-87	34.3323935376515	35.0	34.0	35.5	32.0	37.0
88-89	34.18071532791117	35.0	34.0	35.0	32.0	36.0
90-91	34.13087532465438	35.0	34.0	35.0	32.0	36.0
92-93	34.01782224315136	35.0	34.0	35.0	31.5	36.0
94-95	33.88009694479234	35.0	34.0	35.0	31.0	36.0
96-97	33.91022986417214	35.0	34.0	35.0	31.0	35.5
98-99	33.936919392596835	35.0	34.0	35.0	31.0	35.0
100-101	32.68773037615572	34.0	31.5	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	18.0
21	73.0
22	257.0
23	723.0
24	2057.0
25	4448.0
26	8360.0
27	14295.0
28	21567.0
29	30353.0
30	42404.0
31	57268.0
32	78750.0
33	112034.0
34	184481.0
35	317328.0
36	680240.0
37	1508493.0
38	1903357.0
39	921741.0
40	22831.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.393537191069726	19.48389153053679	12.636144679409847	24.486426598983638
2	15.589914466263835	20.705139450999923	39.99087464204455	23.714071440691686
3	18.732461869289192	25.94077633259342	29.63413093005996	25.69263086805743
4	12.640475527073466	17.05135928559961	38.337187956927686	31.970977230399235
5	13.362745879223825	37.900584376839234	35.28502306599315	13.451646677943788
6	30.938193245771945	37.18049897370231	18.428273270430836	13.453034510094902
7	27.379111408180197	31.99239462548572	22.946421154972786	17.6820728113613
8	25.630074894905047	35.45416296810585	20.932864586743243	17.98289755024587
9	26.40353370280896	15.778165749744547	20.818496789080847	36.999803758365644
10-11	25.080044837966508	26.0563245657041	28.377151178586864	20.48647941774253
12-13	25.665722954700982	23.56932053584343	28.50118971994001	22.263766789515575
14-15	22.79391103119344	24.8325898993467	26.071499926494013	26.301999142965848
16-17	22.501908702686123	27.80493016254382	26.663540336009493	23.02962079876057
18-19	22.419910192430052	27.26996897184492	28.275698378791166	22.034422456933864
20-21	23.30382884458455	26.58156525346315	27.951168589397046	22.163437312555253
22-23	23.211908266190946	26.454648369354004	27.662543065679106	22.670900298775948
24-25	22.82915881284181	26.541342212533937	27.92711819750468	22.702380777119576
26-27	22.794020383141724	26.83526432638755	27.78513365968906	22.58558163078167
28-29	22.62693301626634	27.031048100473352	27.724520637034995	22.61749824622531
30-31	22.984260060965365	26.84741654088586	27.586395036695638	22.581928361453134
32-33	22.771484984335054	26.92963180029223	27.581783969699252	22.717099245673463
34-35	22.80094619331021	27.047424098188877	27.651148347738264	22.50048136076265
36-37	22.874238290763106	26.87634300149731	27.609485053227573	22.639933654512006
38-39	22.724995908188063	26.992989824565296	27.647298294477302	22.634715972769335
40-41	22.86758512282412	26.81058404378083	27.734651646552127	22.58717918684292
42-43	22.883825322731994	26.901158506966468	27.363164596741363	22.851851573560175
44-45	22.8710038224016	27.288493807881014	27.13356346754373	22.706938902173654
46-47	22.911700646060158	27.070747995624018	26.944874252075884	23.07267710623994
48-49	23.033377312431103	26.95796189692028	27.003868669786872	23.004792120861747
50-51	22.76119787109194	27.070885419231605	26.986136426217133	23.18178028345932
52-53	22.92223256653037	27.166510845692983	26.708715044711134	23.202541543065518
54-55	23.03079424826274	27.07446206593143	26.73479105019947	23.159952635606356
56-57	23.11056123885797	27.120499211388456	26.72261210926477	23.04632744048881
58-59	22.95794704213739	27.187611818581747	26.87637585076745	22.978065288513413
60-61	23.145825738636738	27.244638672209803	26.620716848983587	22.988818740169872
62-63	23.08411444414436	27.179346130306946	26.607664168457312	23.128875257091376
64-65	22.87622190878057	27.219491534712297	26.663149917006784	23.241136639500347
66-67	22.978101429095116	27.193485242833816	26.530179612368816	23.298233715702256
68-69	22.97942714755769	27.108549645902812	26.74490398816794	23.167119218371564
70-71	22.98709606890923	26.868058774191805	26.647081812533145	23.497763344365826
72-73	23.07252422065825	27.042866638710432	26.71351539434949	23.17109374628183
74-75	22.944277771127975	27.207355571462738	26.677639911548823	23.170726745860463
76-77	23.01813264226865	27.094215238722175	26.556912012842982	23.3307401061662
78-79	23.023906178531167	27.059562553777504	26.577112073012763	23.33941919467856
80-81	22.888312832186266	27.188566469314186	26.640358939283832	23.28276175921572
82-83	23.186442674860576	27.0193360573384	26.778917613686698	23.015303654114327
84-85	23.332829107958997	26.942065190509723	26.545019150845594	23.180086550685683
86-87	22.894551630537098	27.198066653420867	26.75064428473533	23.156737431306706
88-89	23.169558316914248	26.90582675189673	26.71259194364733	23.2120229875417
90-91	23.238883455938375	26.929160445799223	26.69457382974365	23.13738226851875
92-93	23.303422854057228	27.024909414201947	26.60268229146799	23.068985440272833
94-95	22.964442327655117	27.072475030491734	26.769458634410864	23.193624007442285
96-97	23.043642420376482	27.05548454080403	26.78507450869957	23.115798530119914
98-99	23.316628003306455	27.704994202772966	26.688752208521656	22.289625585398927
100-101	27.862000187012697	34.64753433230127	27.175802248109033	10.314663232577002
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	711.0
1	743.0
2	1428.0
3	2221.0
4	3344.0
5	5929.5
6	7828.5
7	8594.5
8	8668.5
9	7786.0
10	6545.5
11	5638.5
12	6253.0
13	8848.5
14	14610.5
15	21735.5
16	26774.0
17	29389.0
18	29439.0
19	27215.5
20	24185.0
21	21544.5
22	20558.0
23	22315.5
24	26778.5
25	33299.0
26	42304.5
27	52636.5
28	62068.5
29	73057.5
30	83653.5
31	93287.5
32	105552.5
33	119029.5
34	132016.5
35	145174.0
36	161007.5
37	176059.0
38	183398.0
39	187922.5
40	196112.0
41	203386.5
42	208993.5
43	218416.5
44	230783.5
45	244492.0
46	253235.5
47	258729.0
48	266443.5
49	271223.5
50	272954.0
51	273568.5
52	269339.0
53	263700.5
54	255342.0
55	239890.5
56	222651.0
57	203590.0
58	182473.5
59	159387.0
60	132965.0
61	105493.5
62	81834.5
63	63468.0
64	48845.0
65	36400.0
66	26898.5
67	20321.5
68	15621.5
69	11853.5
70	8650.0
71	6293.5
72	4716.0
73	3392.0
74	2444.5
75	1723.0
76	1085.5
77	659.5
78	445.0
79	317.5
80	220.0
81	156.5
82	118.0
83	83.5
84	49.5
85	26.0
86	17.5
87	15.5
88	10.5
89	6.0
90	4.5
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0013872246422525281
3	5.75190705324219E-4
4	0.0
5	0.0
6	5.075212105801932E-4
7	0.0
8	0.0
9	5.075212105801932E-5
10-11	3.552648474061352E-4
12-13	4.736864632081803E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	3.385256109435174E-5
28-29	8.469272210031176E-6
30-31	4.242660854334434E-5
32-33	2.723961334390344E-4
34-35	3.4184507632417746E-5
36-37	7.13368771431145E-4
38-39	1.9071968419594402E-4
40-41	6.12793964924899E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	1.0201772511602198E-4
52-53	0.0
54-55	6.918474497223559E-4
56-57	5.753135818592122E-5
58-59	0.0
60-61	7.171012136299525E-4
62-63	9.940219519807876E-6
64-65	1.005551650663312E-5
66-67	3.053142696460319E-5
68-69	1.0305954945662883E-5
70-71	0.0
72-73	7.403285070260348E-5
74-75	0.0
76-77	3.252374151821476E-5
78-79	5.490999702387816E-5
80-81	0.0
82-83	0.0
84-85	2.2867358686297684E-5
86-87	5.329186762624467E-4
88-89	8.222751036477769E-5
90-91	2.384615604733748E-5
92-93	2.4219986260001795E-5
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	107.0
20-21	376.0
22-23	799.0
24-25	1328.0
26-27	3069.0
28-29	8524.0
30-31	18067.0
32-33	21773.0
34-35	28962.0
36-37	46005.0
38-39	57188.0
40-41	54633.0
42-43	59379.0
44-45	68971.0
46-47	69344.0
48-49	65966.0
50-51	60077.0
52-53	56000.0
54-55	60505.0
56-57	62484.0
58-59	61519.0
60-61	61349.0
62-63	57876.0
64-65	58475.0
66-67	61177.0
68-69	62748.0
70-71	62173.0
72-73	57964.0
74-75	57901.0
76-77	58292.0
78-79	61112.0
80-81	61674.0
82-83	58217.0
84-85	56745.0
86-87	58568.0
88-89	61989.0
90-91	64842.0
92-93	64795.0
94-95	68598.0
96-97	102818.0
98-99	461548.0
100-101	3447146.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.76131127227272
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.14285133722424	72.0667510460845
2	7.677071489290377	12.553748633907217
3	2.2033365108208476	5.404430468963598
4	0.8738189581807073	2.8577833534170347
5	0.4101652660827768	1.6767824996634242
6	0.2316397174128028	1.1363500223045675
7	0.14184935419848635	0.8118452441675315
8	0.08767715867102754	0.5734879569256262
9	0.05434814656162581	0.3999218155286567
>10	0.1734351639506842	2.071610543144745
>50	0.002349750124959554	0.1289243795802292
>100	0.0012352726371083697	0.1997233475133177
>500	2.2187484432964778E-4	0.11864068879947536
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.691737368600644E-5	0.0
5	0.0	0.0	0.0	3.383474737201288E-5	0.0
6	0.0	0.0	0.0	3.383474737201288E-5	0.0
7	0.0	0.0	0.0	3.383474737201288E-5	0.0
8	0.0	0.0	0.0	3.383474737201288E-5	0.0
9	0.0	0.0	0.0	3.383474737201288E-5	0.0
10-11	1.691737368600644E-5	0.0	0.0	6.766949474402576E-5	0.0
12-13	1.691737368600644E-5	0.0	0.0	6.766949474402576E-5	0.0
14-15	1.691737368600644E-5	0.0	0.0	7.612818158702898E-5	0.0
16-17	1.691737368600644E-5	0.0	0.0	9.304555527303541E-5	0.0
18-19	1.691737368600644E-5	0.0	0.0	1.1842161580204507E-4	0.0
20-21	1.691737368600644E-5	0.0	0.0	1.353389894880515E-4	0.0
22-23	1.691737368600644E-5	0.0	0.0	2.0300848423207725E-4	0.0
24-25	1.691737368600644E-5	0.0	0.0	2.4530191844709335E-4	0.0
26-27	1.691737368600644E-5	0.0	0.0	3.552648474061352E-4	0.0
28-29	1.691737368600644E-5	0.0	0.0	9.135381790443477E-4	0.0
30-31	1.691737368600644E-5	0.0	0.0	0.0035780245345903615	0.0
32-33	1.691737368600644E-5	0.0	0.0	0.007748157148190949	0.0
34-35	1.691737368600644E-5	0.0	0.0	0.012645736830289814	0.0
36-37	1.691737368600644E-5	0.0	0.0	0.019920207515272584	0.0
38-39	1.691737368600644E-5	0.0	0.0	0.030679657179572675	0.0
40-41	1.691737368600644E-5	0.0	0.0	0.04205659098341201	0.0
42-43	1.691737368600644E-5	0.0	0.0	0.05184329166076673	0.0
44-45	1.691737368600644E-5	0.0	0.0	0.06218826566975967	0.0
46-47	1.691737368600644E-5	0.0	0.0	0.07357365816044201	0.0
48-49	1.691737368600644E-5	0.0	0.0	0.08452765762213117	0.0
50-51	1.691737368600644E-5	0.0	0.0	0.09505026405482717	0.0
52-53	1.691737368600644E-5	0.0	0.0	0.10562362260858121	0.0
54-55	1.691737368600644E-5	0.0	0.0	0.1150635171253728	0.0
56-57	1.691737368600644E-5	0.0	0.0	0.12446957689479238	0.0
58-59	1.691737368600644E-5	0.0	0.0	0.1335034544431198	0.0
60-61	1.691737368600644E-5	0.0	0.0	0.1421820671440411	0.0
62-63	1.691737368600644E-5	0.0	0.0	0.15041236944228326	0.0
64-65	1.691737368600644E-5	0.0	0.0	0.1581689852773172	0.0
66-67	1.691737368600644E-5	0.0	0.0	0.16620473777817027	0.0
68-69	1.691737368600644E-5	0.0	0.0	0.17401210573426223	0.0
70-71	1.691737368600644E-5	0.0	0.0	0.18170105207455217	0.0
72-73	1.691737368600644E-5	0.0	0.0	0.1878166826620435	0.0
74-75	1.691737368600644E-5	0.0	0.0	0.1925958407283403	0.0
76-77	1.691737368600644E-5	0.0	0.0	0.19637687374716276	0.0
78-79	1.691737368600644E-5	0.0	0.0	0.19979418323173603	0.0
80-81	1.691737368600644E-5	0.0	0.0	0.20140133373190666	0.0
82-83	1.691737368600644E-5	0.0	0.0	0.2020272765582889	0.0
84-85	1.691737368600644E-5	0.0	0.0	0.2025178803951831	0.0
86-87	1.691737368600644E-5	0.0	0.0	0.20273780625310117	0.0
88-89	1.691737368600644E-5	0.0	0.0	0.2028223931215312	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6905	0.0	42.72657	1
GTATCAA	12050	0.0	33.939995	1
ATCAACG	14205	0.0	27.769863	3
CAACGCA	14465	0.0	27.2423	5
TATCAAC	14765	0.0	27.10974	2
TCAACGC	14720	0.0	26.967419	4
AACGCAG	15255	0.0	25.913227	6
GTACATG	20040	0.0	23.695648	1
TACATGG	19910	0.0	23.642248	2
ACGCAGA	16810	0.0	23.318937	7
ACATGGG	19880	0.0	23.135805	3
CGCAGAG	17715	0.0	22.057482	8
CATGGGG	14885	0.0	20.432848	4
GTACTAG	1830	0.0	19.472828	1
GCAGAGT	20965	0.0	18.638124	9
ACGGTAC	695	0.0	17.886051	3
GTACTGG	3505	0.0	17.851332	1
GAGTACT	10515	0.0	17.851255	12-13
AGAGTAC	18520	0.0	16.99301	10-11
TAACGCC	670	0.0	16.698238	4
>>END_MODULE