FastQCFastQC Report
Fri 27 May 2016
SRR2140088_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140088_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6905386
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102000.040.1214681
GTATCAA170000.033.8217961
ATCAACG201850.027.6776983
CAACGCA204300.027.226595
TCAACGC209650.026.6871854
AACGCAG213000.026.4397056
TATCAAC215900.026.2918432
TACATGG263700.023.7033182
GTACATG276650.023.256121
ACATGGG263800.023.2000433
ACGCAGA243700.022.908637
CGCAGAG244650.022.8030288
CATGGGG184350.020.7853534
GTACTAG24650.020.6492371
GCAGAGT295400.018.7475979
GAGTACT148800.018.11608512-13
AGAGTAC262700.017.21363410-11
CAGAGTA276900.017.16176410-11
CGACGAG11350.016.7317724-25
TAGTACT28550.016.6874754
Produced by FastQC (version 0.11.5)