##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140088_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6905386
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79363499737741	34.0	31.0	34.0	31.0	34.0
2	32.99399685984245	34.0	33.0	34.0	31.0	34.0
3	33.10040307667088	34.0	33.0	34.0	31.0	34.0
4	36.468631731810504	37.0	37.0	37.0	35.0	37.0
5	36.33537632798514	37.0	37.0	37.0	35.0	37.0
6	36.36510211014996	37.0	37.0	37.0	35.0	37.0
7	36.37366223988058	37.0	37.0	37.0	35.0	37.0
8	36.38886993428029	37.0	37.0	37.0	35.0	37.0
9	38.30485768644939	39.0	39.0	39.0	37.0	39.0
10-11	38.21258232342117	39.0	39.0	39.0	37.0	39.0
12-13	38.19394158414896	39.0	39.0	39.0	37.0	39.0
14-15	39.7066754849041	41.0	40.0	41.0	37.5	41.0
16-17	39.68833863885379	41.0	40.0	41.0	37.5	41.0
18-19	39.73417503380695	41.0	40.0	41.0	38.0	41.0
20-21	39.75444594578788	41.0	40.0	41.0	38.0	41.0
22-23	39.704099034047054	41.0	40.0	41.0	37.5	41.0
24-25	39.67630094529119	41.0	40.0	41.0	37.0	41.0
26-27	39.56106053343463	41.0	39.5	41.0	37.0	41.0
28-29	39.50228252732925	41.0	39.0	41.0	37.0	41.0
30-31	39.42968298037126	40.5	39.0	41.0	36.5	41.0
32-33	39.33987123389528	40.0	39.0	41.0	36.0	41.0
34-35	39.302099332779065	40.0	39.0	41.0	36.0	41.0
36-37	39.26211747497756	40.0	39.0	41.0	35.5	41.0
38-39	39.195432178087295	40.0	39.0	41.0	35.0	41.0
40-41	39.131285891622596	40.0	39.0	41.0	35.0	41.0
42-43	39.12063469395229	40.0	39.0	41.0	35.0	41.0
44-45	39.0582035683876	40.0	39.0	41.0	35.0	41.0
46-47	38.940072073310716	40.0	38.0	41.0	35.0	41.0
48-49	38.923930374234395	40.0	38.0	41.0	35.0	41.0
50-51	38.806791460794614	40.0	38.0	41.0	35.0	41.0
52-53	38.63918045482516	40.0	38.0	41.0	35.0	41.0
54-55	38.427394282695516	40.0	37.5	41.0	34.5	41.0
56-57	38.26360121212931	40.0	37.0	41.0	34.0	41.0
58-59	38.09142234355495	39.5	36.5	41.0	34.0	41.0
60-61	37.845879679120166	39.0	36.0	41.0	34.0	41.0
62-63	37.66694386056042	39.0	35.5	41.0	34.0	41.0
64-65	37.39237559923389	39.0	35.0	40.5	33.5	41.0
66-67	37.1484898264563	38.0	35.0	40.0	33.0	41.0
68-69	36.88535659667758	37.0	35.0	40.0	33.0	41.0
70-71	36.440364593808184	37.0	35.0	39.5	33.0	41.0
72-73	36.18252345687603	36.0	35.0	39.0	33.0	41.0
74-75	35.82946846011076	36.0	35.0	39.0	32.5	40.0
76-77	34.06590697191395	34.5	33.0	36.5	30.0	39.0
78-79	35.060129716250046	35.0	34.0	37.0	32.0	39.0
80-81	35.00320172027831	35.0	34.5	37.0	32.0	39.0
82-83	34.74849853137677	35.0	34.0	36.0	32.0	38.0
84-85	34.52290547410297	35.0	34.0	36.0	32.0	37.0
86-87	34.357392244232855	35.0	34.0	35.5	32.0	37.0
88-89	34.20922582764557	35.0	34.0	35.0	32.0	36.0
90-91	34.15646144458666	35.0	34.0	35.0	32.0	36.0
92-93	34.04760576703745	35.0	34.0	35.0	32.0	36.0
94-95	33.91038601822378	35.0	34.0	35.0	31.0	36.0
96-97	33.94250976703767	35.0	34.0	35.0	31.0	35.5
98-99	33.97566979538135	35.0	34.0	35.0	31.5	35.0
100-101	32.73181755827879	34.0	31.5	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	10.0
20	27.0
21	86.0
22	224.0
23	870.0
24	2383.0
25	5168.0
26	9558.0
27	15990.0
28	24433.0
29	34764.0
30	47039.0
31	64421.0
32	87642.0
33	127121.0
34	211549.0
35	362483.0
36	781229.0
37	1726041.0
38	2232222.0
39	1142863.0
40	29260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.08100951923615	19.936814538680387	12.702823564099097	24.279352377984374
2	15.416738049437958	21.011139187932216	40.21784259856362	23.35428016406621
3	18.414192953803013	26.585446455801897	30.031532109488566	24.96882848090652
4	12.397105679537683	17.490086723609657	38.693970185012105	31.418837411840556
5	13.140165247846427	38.14557769045462	35.396520251948296	13.317736809750652
6	30.16401338614069	37.67720134718713	18.715761153922518	13.443024112749663
7	26.715783882320267	32.38172348366912	23.316712490800658	17.585780143209952
8	25.46082145154521	35.36939137073583	21.305876311621102	17.863910866097854
9	26.42961978224241	15.507216469465495	21.047240667971444	37.015923080320654
10-11	24.882118856399586	26.16593196030942	28.586064186455562	20.365884996835433
12-13	25.549749190971905	23.913764878220935	28.548705001127022	21.987780929680138
14-15	22.706435237653622	25.05929429578593	26.279559173086053	25.954711293474396
16-17	22.164285964607917	27.927584352272273	26.966385369333445	22.94174431378637
18-19	21.95415288877407	27.39147384375037	28.7331005102394	21.921272757236164
20-21	22.955015833315052	26.674919845041835	28.37289652745963	21.997167794183486
22-23	22.798995064391452	26.747452938359128	28.09773197208113	22.35582002516829
24-25	22.4637899484579	26.834113183452818	28.47093657481368	22.231160293275597
26-27	22.415607087879554	26.97815227058703	28.259861484733378	22.346379156800033
28-29	22.074663710068844	27.26146793545454	28.204818420505063	22.459049933971553
30-31	22.55032362128214	26.897649006002194	28.12594167773626	22.42608569497941
32-33	22.150155004073685	27.123541234581577	28.16431306304665	22.56199069829809
34-35	22.382626683180444	27.264869390307524	28.233418786612248	22.11908513989979
36-37	22.570189450762122	27.050867760270236	28.00817247828702	22.37077031068062
38-39	22.273148351751797	27.098180224920803	28.229511224497468	22.399160198829936
40-41	22.48694194123977	26.868454280150882	28.321659094678868	22.322944683930473
42-43	22.577827431071825	26.961107353646764	27.8735931186376	22.58747209664381
44-45	22.41037978954764	27.500302374079492	27.64514918790411	22.444168648468754
46-47	22.635170154741278	27.16319698888264	27.29818695375304	22.903445902623048
48-49	22.679793339348777	27.251890662687384	27.252667701661437	22.815648296302406
50-51	22.550736011347684	27.194072879044395	27.261997920534053	22.993193189073864
52-53	22.65013771863087	27.305767908507633	27.005927800448347	23.03816657241315
54-55	22.65678397064928	27.35298829682468	26.928261018828454	23.06196671369759
56-57	22.842394226394806	27.41322615332687	26.78365590508426	22.96072371519407
58-59	22.599255215674557	27.4724964588903	27.206191827719593	22.72205649771555
60-61	22.829093597858378	27.591699134395668	26.87624698522103	22.70296028252492
62-63	22.768393947375117	27.4092890846443	26.83065451945691	22.991662448523677
64-65	22.63367531216328	27.50331337650008	26.84436997085426	23.018641340482375
66-67	22.747736506205438	27.49922296408331	26.693837848475273	23.059202681235984
68-69	22.67508110811857	27.398833159668868	26.865658926370223	23.06042680584234
70-71	22.758320316474805	27.040837637376235	26.821944647821677	23.37889739832728
72-73	22.756145361102018	27.21728108841678	26.95948077345509	23.06709277702611
74-75	22.698736134820365	27.268205550767423	26.936192909086227	23.096865405325985
76-77	22.860692475155556	27.118121222528398	26.79075155478263	23.23043474753342
78-79	22.711204888875848	27.160420307965026	26.804491261419987	23.323883541739136
80-81	22.62869736367921	27.124055981719607	26.961934331573673	23.28531232302751
82-83	23.00958939523337	26.926937318369333	27.070018780447814	22.99345450594949
84-85	23.10506547732773	27.093688114477004	26.76601810374767	23.035228304447593
86-87	22.771681595774574	27.17900517083883	26.944238511810394	23.105074721576198
88-89	23.016460209528052	26.956122788137947	26.842820248233167	23.18459675410083
90-91	23.06871704684889	26.86663068204716	26.937338744459755	23.127313526644194
92-93	23.145373807900327	26.791433571452195	27.03919405095424	23.023998569693234
94-95	22.808578426084487	26.780868185404287	27.270327097035217	23.140226291476
96-97	22.83851323104569	26.862389296951623	27.209934331175763	23.089163140826926
98-99	23.114166788493375	27.471928825355718	27.116006047324454	22.29789833882645
100-101	27.498660825115966	34.30094763991249	27.809146737180424	10.391244797791117
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	740.0
1	884.0
2	1963.0
3	3091.5
4	4657.5
5	8208.0
6	10753.0
7	11845.0
8	12006.5
9	10918.0
10	9291.0
11	7938.5
12	8958.5
13	13029.5
14	21587.5
15	32123.0
16	39812.0
17	43428.0
18	43001.0
19	39641.5
20	34910.5
21	30454.0
22	28065.5
23	29192.5
24	33641.5
25	41132.0
26	51616.5
27	63030.0
28	75313.0
29	91426.5
30	106538.0
31	120129.0
32	136887.0
33	154031.0
34	171618.0
35	189704.0
36	206700.5
37	224232.5
38	235615.5
39	239586.0
40	242254.0
41	243545.0
42	246918.0
43	257634.5
44	272850.0
45	288892.5
46	301762.0
47	312306.0
48	317700.5
49	316899.5
50	312828.0
51	309231.0
52	305284.0
53	298413.5
54	292483.0
55	278864.0
56	258967.5
57	231479.5
58	201257.0
59	172500.5
60	140402.0
61	113024.5
62	89895.5
63	68827.0
64	51090.0
65	37757.5
66	28565.5
67	22191.0
68	17294.5
69	13178.5
70	9657.5
71	6324.0
72	4368.0
73	2909.5
74	1855.0
75	1211.0
76	777.5
77	494.5
78	322.0
79	217.0
80	147.0
81	102.5
82	73.5
83	54.0
84	37.5
85	21.5
86	13.0
87	9.0
88	5.0
89	2.5
90	2.0
91	2.5
92	2.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0018101812121726433
3	6.371837866847704E-4
4	0.0
5	1.4481449697381145E-5
6	5.068507394083401E-4
7	0.0
8	0.0
9	1.4481449697381145E-5
10-11	2.823882690989323E-4
12-13	4.706471151648872E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	7.244441285868044E-5
28-29	1.449501925772021E-5
30-31	5.8089002517432144E-5
32-33	2.477727055628033E-4
34-35	5.1245586199358655E-5
36-37	7.958133145757187E-4
38-39	2.4556399799619775E-4
40-41	8.274704544151792E-5
42-43	0.0
44-45	7.703797610143313E-6
46-47	1.5634671510079439E-4
48-49	0.0
50-51	4.5813627110671975E-4
52-53	0.0
54-55	7.82104590764596E-4
56-57	1.0006006939499346E-4
58-59	8.449822587524953E-6
60-61	9.765064539367344E-4
62-63	1.7367544037361756E-5
64-65	8.801636893222854E-6
66-67	9.821760055830456E-5
68-69	3.6252463241588316E-5
70-71	0.0
72-73	1.400325659735428E-4
74-75	0.0
76-77	7.683149968556709E-5
78-79	1.461958950921648E-4
80-81	0.0
82-83	3.0177464616168302E-5
84-85	2.042320766695385E-5
86-87	4.7695498664733407E-4
88-89	1.1588344906227113E-4
90-91	2.143642481592006E-5
92-93	1.09150382157318E-5
94-95	1.112128461513738E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	89.0
20-21	395.0
22-23	865.0
24-25	1628.0
26-27	2570.0
28-29	8296.0
30-31	23557.0
32-33	29364.0
34-35	39055.0
36-37	61166.0
38-39	74066.0
40-41	71889.0
42-43	79775.0
44-45	92702.0
46-47	91727.0
48-49	87066.0
50-51	78794.0
52-53	70514.0
54-55	75898.0
56-57	78835.0
58-59	79013.0
60-61	81341.0
62-63	76654.0
64-65	79282.0
66-67	83636.0
68-69	81995.0
70-71	80954.0
72-73	74100.0
74-75	75364.0
76-77	74921.0
78-79	79359.0
80-81	81063.0
82-83	74765.0
84-85	73718.0
86-87	75139.0
88-89	79939.0
90-91	84526.0
92-93	83617.0
94-95	86577.0
96-97	119085.0
98-99	515600.0
100-101	3796487.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.3989071770789
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.64441057431281	69.58870419779656
2	7.629488294986117	12.115460658844253
3	2.25420055988245	5.369431830378779
4	0.9863539253893902	3.132616950629584
5	0.52015339763208	2.0649805668215864
6	0.30122667296510813	1.4350241187610153
7	0.17255768446066808	0.9590624099828977
8	0.12018149154489302	0.7633823273260681
9	0.08770607577548799	0.6267389912427437
>10	0.2779180879136035	3.380677778559045
>50	0.004310160974967102	0.22484422991038708
>100	0.0012657363420808018	0.20216384917343622
>500	2.2733782044611524E-4	0.13691209057364073
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	1.4481449697381145E-5	0.0	0.0	0.0	0.0
3	1.4481449697381145E-5	0.0	0.0	0.0	0.0
4	1.4481449697381145E-5	0.0	0.0	0.0	0.0
5	5.792579878952458E-5	0.0	0.0	0.0	0.0
6	1.0137014788166803E-4	0.0	0.0	0.0	0.0
7	1.1585159757904916E-4	0.0	0.0	0.0	0.0
8	1.1585159757904916E-4	0.0	0.0	0.0	0.0
9	1.1585159757904916E-4	0.0	0.0	0.0	0.0
10-11	1.1585159757904916E-4	0.0	0.0	0.0	0.0
12-13	1.4481449697381147E-4	0.0	0.0	7.240724848690573E-6	0.0
14-15	2.461846448554795E-4	0.0	0.0	3.620362424345286E-5	0.0
16-17	2.5342536970417005E-4	0.0	0.0	6.516652363821516E-5	0.0
18-19	3.692769672832192E-4	0.0	0.0	1.8825884606595488E-4	0.0
20-21	4.344434909214344E-4	0.0	0.0	6.661466860795327E-4	0.0
22-23	4.6340639031619666E-4	0.0	0.0	0.0015350336679224013	0.0
24-25	5.502950885004835E-4	0.0	0.0	0.003685528947983502	0.0
26-27	5.502950885004835E-4	0.0	0.0	0.008413722274178446	0.0
28-29	6.95109585474295E-4	0.0	0.0	0.04491421623642762	0.0
30-31	7.530353842638195E-4	0.0	0.0	0.06469587652305027	0.0
32-33	7.602761091125101E-4	0.0	0.0	0.06971369884319284	0.0
34-35	8.544055321454875E-4	0.0	7.240724848690573E-6	0.07570901901790864	0.0
36-37	9.48534955178465E-4	7.240724848690573E-6	1.4481449697381145E-5	0.0843327223126991	0.0
38-39	0.001100590177000967	1.4481449697381145E-5	1.4481449697381145E-5	0.1015366845531879	0.0
40-41	0.0011078309018496576	1.4481449697381145E-5	1.4481449697381145E-5	0.14052798786338666	0.0
42-43	0.001201960324882635	1.4481449697381145E-5	1.4481449697381145E-5	0.15655895267838754	0.0
44-45	0.0013178119224616842	1.4481449697381145E-5	1.4481449697381145E-5	0.17615959484379295	0.0
46-47	0.0015495151176197826	1.4481449697381145E-5	1.4481449697381145E-5	0.19730251140196942	0.0
48-49	0.001694329614593594	1.4481449697381145E-5	1.4481449697381145E-5	0.2158242855649199	0.0
50-51	0.001694329614593594	1.4481449697381145E-5	1.4481449697381145E-5	0.23631553688671422	0.0
52-53	0.001694329614593594	1.4481449697381145E-5	1.4481449697381145E-5	0.2618535734280459	0.0
54-55	0.001694329614593594	1.4481449697381145E-5	1.4481449697381145E-5	0.3101709303433581	0.0
56-57	0.0017522554133831186	1.4481449697381145E-5	1.4481449697381145E-5	0.36288340724182544	0.0
58-59	0.0017522554133831186	1.4481449697381145E-5	1.4481449697381145E-5	0.4156972542881745	0.0
60-61	0.0017667368630804997	1.4481449697381145E-5	1.4481449697381145E-5	0.4504599742867379	0.0
62-63	0.0017812183127778808	1.4481449697381145E-5	1.4481449697381145E-5	0.46718604868721314	0.0
64-65	0.0018246626618700244	1.4481449697381145E-5	1.4481449697381145E-5	0.4822177934730948	0.0
66-67	0.001983958608541217	1.4481449697381145E-5	1.4481449697381145E-5	0.49058807139818106	0.0
68-69	0.0020418844073307415	1.4481449697381145E-5	1.4481449697381145E-5	0.4991393674444846	0.0
70-71	0.002063606581876813	1.4481449697381145E-5	1.4481449697381145E-5	0.5070317575295573	0.0
72-73	0.0020780880315741943	1.4481449697381145E-5	1.4481449697381145E-5	0.5128533003079045	0.0
74-75	0.002099810206120266	1.4481449697381145E-5	1.4481449697381145E-5	0.517639419432889	0.0
76-77	0.002099810206120266	1.4481449697381145E-5	1.4481449697381145E-5	0.5218680027445244	0.0
78-79	0.0021215323806663376	1.4481449697381145E-5	1.4481449697381145E-5	0.5257345498137251	0.0
80-81	0.002338754126127055	1.4481449697381145E-5	1.4481449697381145E-5	0.5272044169580092	0.0
82-83	0.00240392064976527	1.4481449697381145E-5	1.4481449697381145E-5	0.5278053971204506	0.0
84-85	0.002440124274008723	1.4481449697381145E-5	1.4481449697381145E-5	0.5282108777119773	0.0
86-87	0.0024980500727982474	1.4481449697381145E-5	1.4481449697381145E-5	0.5283267293095564	0.0
88-89	0.0025197722473443195	1.4481449697381145E-5	1.4481449697381145E-5	0.5284570623568328	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10200	0.0	40.121468	1
GTATCAA	17000	0.0	33.821796	1
ATCAACG	20185	0.0	27.677698	3
CAACGCA	20430	0.0	27.22659	5
TCAACGC	20965	0.0	26.687185	4
AACGCAG	21300	0.0	26.439705	6
TATCAAC	21590	0.0	26.291843	2
TACATGG	26370	0.0	23.703318	2
GTACATG	27665	0.0	23.25612	1
ACATGGG	26380	0.0	23.200043	3
ACGCAGA	24370	0.0	22.90863	7
CGCAGAG	24465	0.0	22.803028	8
CATGGGG	18435	0.0	20.785353	4
GTACTAG	2465	0.0	20.649237	1
GCAGAGT	29540	0.0	18.747597	9
GAGTACT	14880	0.0	18.116085	12-13
AGAGTAC	26270	0.0	17.213634	10-11
CAGAGTA	27690	0.0	17.161764	10-11
CGACGAG	1135	0.0	16.73177	24-25
TAGTACT	2855	0.0	16.687475	4
>>END_MODULE