FastQCFastQC Report
Fri 27 May 2016
SRR2140087_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140087_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4742102
Sequences flagged as poor quality0
Sequence length19-101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53050.037.6049961
GTATCAA83650.032.864691
ATCAACG99050.026.8543823
CAACGCA99500.026.8144325
TCAACGC103150.025.9442124
TATCAAC105200.025.7084482
AACGCAG108450.024.938296
TACATGG149900.023.560382
GTACATG154150.023.0423281
ACATGGG154300.022.4943583
ACGCAGA121950.021.9443827
CGCAGAG129200.020.6502158
CATGGGG116950.019.623754
GTACTAG12500.018.4897331
GCAGAGT156600.016.8558439
GTATAAG22650.016.469691
AGAGTAC137200.015.766726510-11
GAGTACT71950.015.58200112-13
ATGGGGG82850.015.416435
CAGAGTA148450.015.3776310-11
Produced by FastQC (version 0.11.5)