##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140087_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4742102
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.78436693263873	34.0	31.0	34.0	31.0	34.0
2	32.988488860003436	34.0	33.0	34.0	31.0	34.0
3	33.096722508288515	34.0	33.0	34.0	31.0	34.0
4	36.465040819450955	37.0	37.0	37.0	35.0	37.0
5	36.324603097951076	37.0	37.0	37.0	35.0	37.0
6	36.35362693590311	37.0	37.0	37.0	35.0	37.0
7	36.36196184729894	37.0	37.0	37.0	35.0	37.0
8	36.37332284290806	37.0	37.0	37.0	35.0	37.0
9	38.28307046115837	39.0	39.0	39.0	37.0	39.0
10-11	38.19135490970038	39.0	39.0	39.0	37.0	39.0
12-13	38.17840717049106	39.0	39.0	39.0	37.0	39.0
14-15	39.69695021321768	41.0	40.0	41.0	37.5	41.0
16-17	39.67490418805838	41.0	40.0	41.0	37.0	41.0
18-19	39.70389755429132	41.0	40.0	41.0	38.0	41.0
20-21	39.71783778303568	41.0	40.0	41.0	38.0	41.0
22-23	39.67121573108747	41.0	40.0	41.0	37.0	41.0
24-25	39.65025082920663	41.0	40.0	41.0	37.0	41.0
26-27	39.539036612561134	41.0	39.0	41.0	37.0	41.0
28-29	39.47730325814848	41.0	39.0	41.0	37.0	41.0
30-31	39.41949329248352	40.0	39.0	41.0	37.0	41.0
32-33	39.34281005101141	40.0	39.0	41.0	36.0	41.0
34-35	39.30627844182343	40.0	39.0	41.0	36.0	41.0
36-37	39.270495448285345	40.0	39.0	41.0	36.0	41.0
38-39	39.21115845802906	40.0	39.0	41.0	35.5	41.0
40-41	39.14367932776253	40.0	39.0	41.0	35.0	41.0
42-43	39.127876147350904	40.0	39.0	41.0	35.0	41.0
44-45	39.06228707844764	40.0	39.0	41.0	35.0	41.0
46-47	38.94591113204393	40.0	38.0	41.0	35.0	41.0
48-49	38.92718912645011	40.0	38.0	41.0	35.0	41.0
50-51	38.805508088703576	40.0	38.0	41.0	35.0	41.0
52-53	38.62528514041866	40.0	38.0	41.0	35.0	41.0
54-55	38.411272236240755	40.0	37.5	41.0	34.5	41.0
56-57	38.24162659421687	40.0	37.0	41.0	34.0	41.0
58-59	38.05268155738747	39.5	36.5	41.0	34.0	41.0
60-61	37.794200561145736	39.0	36.0	41.0	34.0	41.0
62-63	37.599434039760084	39.0	35.5	41.0	34.0	41.0
64-65	37.29737214685436	38.5	35.0	40.0	33.5	41.0
66-67	37.02892554346482	37.5	35.0	40.0	33.0	41.0
68-69	36.743681294491566	37.0	35.0	40.0	33.0	41.0
70-71	36.28521754773591	36.5	35.0	39.0	33.0	41.0
72-73	36.01878925958371	36.0	35.0	39.0	33.0	41.0
74-75	35.66370884488161	35.5	35.0	38.5	32.5	40.0
76-77	33.9130159974617	34.5	33.0	36.0	30.0	39.0
78-79	34.91362009461882	35.0	34.0	37.0	31.5	39.0
80-81	34.85906300043969	35.0	34.0	36.0	32.0	39.0
82-83	34.6233880133558	35.0	34.0	36.0	32.0	37.0
84-85	34.41381274942965	35.0	34.0	36.0	32.0	37.0
86-87	34.26037401669667	35.0	34.0	35.0	32.0	37.0
88-89	34.123809653291836	35.0	34.0	35.0	31.5	36.0
90-91	34.07826761930849	35.0	34.0	35.0	32.0	36.0
92-93	33.97457256945324	35.0	34.0	35.0	31.0	36.0
94-95	33.842108634471984	35.0	34.0	35.0	31.0	35.5
96-97	33.87962334244861	35.0	34.0	35.0	31.0	35.0
98-99	33.91374684920936	35.0	34.0	35.0	31.0	35.0
100-101	32.64921008890342	34.0	31.5	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	8.0
20	14.0
21	54.0
22	221.0
23	622.0
24	1608.0
25	3432.0
26	6754.0
27	11308.0
28	17019.0
29	24513.0
30	33745.0
31	45191.0
32	62420.0
33	89928.0
34	147468.0
35	254937.0
36	543515.0
37	1204414.0
38	1503733.0
39	771329.0
40	19867.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.50195335317545	19.271053216485008	12.488301601273022	24.73869182906652
2	15.84588401740264	20.52604375459586	39.578396550513396	24.049675677488104
3	19.028986460782154	25.453535540130524	29.31810877767459	26.199369221412734
4	13.015135482113207	16.81806928657376	37.78862200770882	32.378173223604215
5	13.74765873867749	37.50824001676894	35.08180549469412	13.662295749859451
6	31.55751437925381	36.63265553581502	18.18644791573309	13.623382169198084
7	27.85389264085842	31.532725361031883	22.679731477728655	17.933650520381047
8	25.860557195943905	35.226825572288405	20.71849150440037	18.194125727367314
9	26.49415807589124	15.627373683653367	20.544813249483035	37.333654990972356
10-11	25.388613382996443	25.749351366471856	28.153642137756087	20.708393112775617
12-13	25.85633982003491	23.266330127044288	28.236948018556813	22.640382034363988
14-15	23.026212848226375	24.478438042876345	25.615170234634345	26.880178874262935
16-17	22.896228297071637	27.522457340647673	26.278357150478836	23.302957211801857
18-19	22.912254945169884	27.0206334659187	27.740925859460635	22.326185729450778
20-21	23.65758097597577	26.35108632249689	27.311501020041916	22.679831681485428
22-23	23.599190905852645	26.209470686998205	27.126018037375154	23.065320369773996
24-25	23.220515663191392	26.375080047686904	27.258874008526607	23.145530280595093
26-27	23.21573021759871	26.6066297834309	27.177231951273956	23.000408047696432
28-29	23.06148572374564	26.72855880125834	27.120280013835142	23.089675461160876
30-31	23.30312607504828	26.590743413045942	27.001953512518362	23.10417699938742
32-33	23.163491299965496	26.699521612833593	26.999830877516228	23.137156209684683
34-35	23.13099050399913	26.84516435747385	27.103458228205874	22.920386910321145
36-37	23.222699908469846	26.684367154702088	26.990614262472796	23.10231867435527
38-39	23.209878072540572	26.73541555490865	27.004030418242685	23.05067595430809
40-41	23.31664215963918	26.488342756716154	27.210004146215123	22.985010937429546
42-43	23.285917607247338	26.575140182963946	26.861292587634523	23.277649622154193
44-45	23.2528295777741	26.941077501051836	26.609394933253128	23.196697987920942
46-47	23.31972965623344	26.812560412006885	26.500502973394752	23.36720695836492
48-49	23.44125249997388	26.66933253163925	26.440803288183872	23.448611680202994
50-51	23.116954242984377	26.767054176456245	26.55245945121531	23.56353212934406
52-53	23.25089202440186	26.815112950394344	26.30657584178559	23.627419183418205
54-55	23.423046114922567	26.689662280816762	26.33086834946454	23.556423254796133
56-57	23.507148019501216	26.69209905692168	26.358194777373868	23.44255814620324
58-59	23.384719195210085	26.762355057708405	26.555250243777035	23.297675503304475
60-61	23.500518217312266	26.87340792265862	26.173417280586236	23.45265657944288
62-63	23.543780978162804	26.823126303498174	26.16905779069424	23.464034927644782
64-65	23.338349216964833	26.80919811962688	26.23286401770983	23.619588645698457
66-67	23.439755647497332	26.782819096085	26.13392861836744	23.643496638050227
68-69	23.37617153166551	26.76884428878771	26.304905680707556	23.55007849883922
70-71	23.442299433706157	26.474213470622924	26.240392758162713	23.84309433750821
72-73	23.469648006783665	26.609166489150354	26.28684905592224	23.634336448143742
74-75	23.285418857099092	26.76695655806764	26.342048643266462	23.605575941566805
76-77	23.478975559032644	26.68137759360949	26.106094945224562	23.733551902133307
78-79	23.380851410488866	26.696278709922964	26.143048322790662	23.779821556797504
80-81	23.345128857726422	26.75392532288084	26.239413889059026	23.661531930333712
82-83	23.74801435351929	26.557097629187954	26.363772650770663	23.33111536652209
84-85	23.700487556928596	26.588785830998646	26.119076219281933	23.59165039279082
86-87	23.337723677171965	26.784300714325465	26.322371868306714	23.555603740195856
88-89	23.5754443299787	26.468547973066215	26.224460152698054	23.73154754425703
90-91	23.64782473031795	26.509937875434293	26.181360559521128	23.66087683472663
92-93	23.75768985322272	26.536104020421185	26.23165283982131	23.47455328653478
94-95	23.456699166890342	26.66118054322683	26.382725429529064	23.49939486035376
96-97	23.420431985406623	26.57606126360148	26.331713501090565	23.67179324990133
98-99	23.764300578289426	27.1004996508779	26.330554587684734	22.804645183147937
100-101	28.48259875818196	34.27102456061116	26.622210846362464	10.624165834844408
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	419.0
1	452.0
2	898.0
3	1391.5
4	2050.0
5	3585.0
6	4725.0
7	5253.5
8	5355.5
9	4865.0
10	4156.5
11	3567.0
12	3895.5
13	5407.5
14	8775.5
15	13054.5
16	16288.5
17	18221.0
18	18563.5
19	17483.0
20	15959.5
21	14632.0
22	14301.0
23	15751.0
24	19073.5
25	23769.5
26	29808.5
27	36963.0
28	44291.0
29	52602.5
30	60764.5
31	68242.5
32	77833.0
33	88949.5
34	99476.5
35	110892.5
36	124587.0
37	137083.5
38	144723.0
39	150068.5
40	156794.5
41	163168.5
42	169453.5
43	179387.0
44	192076.5
45	205220.0
46	213388.0
47	219419.0
48	228531.5
49	234125.5
50	236314.5
51	237098.5
52	232153.5
53	226549.5
54	221331.5
55	211194.5
56	198312.5
57	184447.0
58	166942.5
59	142886.0
60	116108.0
61	92407.5
62	72923.5
63	57203.5
64	43999.5
65	32608.0
66	24529.0
67	18790.0
68	14180.0
69	10499.5
70	7743.0
71	5737.5
72	4162.0
73	2890.5
74	2006.0
75	1390.0
76	922.0
77	593.0
78	411.5
79	291.0
80	186.5
81	109.0
82	76.0
83	47.0
84	27.0
85	21.0
86	20.0
87	19.5
88	14.0
89	6.5
90	4.5
91	4.0
92	3.5
93	3.5
94	2.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0013285247765653292
3	5.482800665190246E-4
4	0.0
5	0.0
6	5.69367761385141E-4
7	0.0
8	0.0
9	0.0
10-11	3.690346601570359E-4
12-13	3.268592704248032E-4
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	6.329587975470737E-5
28-29	0.0
30-31	4.2327321195903435E-5
32-33	2.5495786821227794E-4
34-35	1.067374036788327E-5
36-37	6.87949458073189E-4
38-39	2.281917175534197E-4
40-41	8.798326734221686E-5
42-43	0.0
44-45	0.0
46-47	0.0
48-49	1.1607538446198653E-5
50-51	3.531840840182554E-5
52-53	0.0
54-55	6.645854267774276E-4
56-57	3.67614397005168E-5
58-59	0.0
60-61	8.449809330683036E-4
62-63	1.2788011597191957E-5
64-65	1.2960937936419082E-5
66-67	2.6285761449880545E-5
68-69	0.0
70-71	0.0
72-73	5.493731995151781E-5
74-75	1.3925926049711936E-5
76-77	1.4124295780267767E-5
78-79	2.865958134943352E-5
80-81	0.0
82-83	0.0
84-85	5.9940574914030233E-5
86-87	4.865746452642754E-4
88-89	7.717941945949401E-5
90-91	1.5687623030183614E-5
92-93	1.5954049783974188E-5
94-95	1.6227931028047547E-5
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	84.0
20-21	304.0
22-23	599.0
24-25	1034.0
26-27	2741.0
28-29	7963.0
30-31	17883.0
32-33	20822.0
34-35	28427.0
36-37	46504.0
38-39	56541.0
40-41	52683.0
42-43	55835.0
44-45	63482.0
46-47	64054.0
48-49	61513.0
50-51	56616.0
52-53	53163.0
54-55	56912.0
56-57	58488.0
58-59	57156.0
60-61	56042.0
62-63	52399.0
64-65	52631.0
66-67	55110.0
68-69	54953.0
70-71	54890.0
72-73	50565.0
74-75	50227.0
76-77	50188.0
78-79	52493.0
80-81	52151.0
82-83	49092.0
84-85	48047.0
86-87	48798.0
88-89	51260.0
90-91	53287.0
92-93	52874.0
94-95	55110.0
96-97	82769.0
98-99	357296.0
100-101	2599116.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.75868782536206
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.1655078875184	74.68385939940512
2	7.183071715402608	12.0328932287519
3	1.988732138206684	4.997207829969554
4	0.7619349663759676	2.5527469196764967
5	0.3586315937296	1.501925585175482
6	0.19637331048996368	0.986878248633706
7	0.10345793396527019	0.6065850555837792
8	0.06283406527453364	0.42103190865024687
9	0.04313478287513859	0.3251621531928156
>10	0.1339012684134381	1.654003520068143
>50	0.001543838705213451	0.0818457892000684
>100	8.764990432227084E-4	0.15586036169265277
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.1087694866116334E-5	0.0
6	0.0	0.0	0.0	2.1087694866116334E-5	0.0
7	0.0	0.0	0.0	2.1087694866116334E-5	0.0
8	0.0	0.0	0.0	4.217538973223267E-5	0.0
9	0.0	0.0	0.0	4.217538973223267E-5	0.0
10-11	0.0	0.0	0.0	6.3263084598349E-5	0.0
12-13	0.0	0.0	0.0	6.3263084598349E-5	0.0
14-15	0.0	0.0	0.0	6.3263084598349E-5	0.0
16-17	0.0	0.0	0.0	6.3263084598349E-5	0.0
18-19	0.0	0.0	0.0	6.3263084598349E-5	0.0
20-21	0.0	0.0	0.0	6.3263084598349E-5	0.0
22-23	0.0	0.0	0.0	1.0543847433058166E-4	0.0
24-25	0.0	0.0	0.0	1.26526169196698E-4	0.0
26-27	0.0	0.0	0.0	2.0033310122810518E-4	0.0
28-29	0.0	0.0	0.0	6.95893930581839E-4	0.0
30-31	0.0	0.0	0.0	0.0022985587404066804	0.0
32-33	0.0	0.0	0.0	0.0056409583766861195	0.0
34-35	0.0	0.0	0.0	0.009194234961626722	0.0
36-37	0.0	0.0	0.0	0.014518877915321096	0.0
38-39	0.0	0.0	0.0	0.02281688584513787	0.0
40-41	0.0	0.0	0.0	0.0315577353671431	0.0
42-43	0.0	0.0	0.0	0.03905441089204745	0.0
44-45	0.0	0.0	0.0	0.04758438346539151	0.0
46-47	0.0	0.0	0.0	0.0573163546461042	0.0
48-49	0.0	0.0	0.0	0.06638406343853422	0.0
50-51	0.0	0.0	0.0	0.0753357899092006	0.0
52-53	0.0	0.0	0.0	0.08391848171970995	0.0
54-55	0.0	0.0	0.0	0.09259606815711682	0.0
56-57	0.0	0.0	0.0	0.10080972530746914	0.0
58-59	0.0	0.0	0.0	0.10865434779766442	0.0
60-61	0.0	0.0	0.0	0.11642516335582828	0.0
62-63	0.0	0.0	0.0	0.12378476886410289	0.0
64-65	0.0	0.0	0.0	0.13084914664425185	0.0
66-67	0.0	0.0	0.0	0.13796624366156612	0.0
68-69	2.1087694866116334E-5	0.0	0.0	0.14528367378010848	0.0
70-71	2.1087694866116334E-5	0.0	0.0	0.15228478847565913	0.0
72-73	2.1087694866116334E-5	0.0	0.0	0.1581577114958725	0.0
74-75	2.1087694866116334E-5	0.0	0.0	0.16242796970626106	0.0
76-77	2.1087694866116334E-5	0.0	0.0	0.1666033332897521	0.0
78-79	2.1087694866116334E-5	0.0	0.0	0.1704096622130861	0.0
80-81	2.1087694866116334E-5	0.0	0.0	0.17238136168306797	0.0
82-83	2.1087694866116334E-5	0.0	0.0	0.17284529097012252	0.0
84-85	2.1087694866116334E-5	0.0	0.0	0.1733092202571771	0.0
86-87	2.1087694866116334E-5	0.0	0.0	0.17347792181610602	0.0
88-89	2.1087694866116334E-5	0.0	0.0	0.17353064105327132	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5305	0.0	37.604996	1
GTATCAA	8365	0.0	32.86469	1
ATCAACG	9905	0.0	26.854382	3
CAACGCA	9950	0.0	26.814432	5
TCAACGC	10315	0.0	25.944212	4
TATCAAC	10520	0.0	25.708448	2
AACGCAG	10845	0.0	24.93829	6
TACATGG	14990	0.0	23.56038	2
GTACATG	15415	0.0	23.042328	1
ACATGGG	15430	0.0	22.494358	3
ACGCAGA	12195	0.0	21.944382	7
CGCAGAG	12920	0.0	20.650215	8
CATGGGG	11695	0.0	19.62375	4
GTACTAG	1250	0.0	18.489733	1
GCAGAGT	15660	0.0	16.855843	9
GTATAAG	2265	0.0	16.46969	1
AGAGTAC	13720	0.0	15.7667265	10-11
GAGTACT	7195	0.0	15.582001	12-13
ATGGGGG	8285	0.0	15.41643	5
CAGAGTA	14845	0.0	15.37763	10-11
>>END_MODULE