FastQCFastQC Report
Fri 27 May 2016
SRR2140086_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140086_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6724245
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC186950.058.85531
TATACAC237750.048.582383
GGTATCA115000.048.5494271
TTATACA236800.048.2591252
GTATCAA217700.034.538921
TACACAT348850.033.1686445
ATACACA369550.031.89654
ACACATC378300.030.2057256
ATCAACG282850.029.5151233
TCAACGC288800.028.9777494
TATCAAC291600.028.9371812
CAACGCA292000.028.64625
TGGTATC49050.028.560682
ACATCTC402800.028.3581358
CACATCT405850.028.1752097
AACGCAG301100.027.859136
GTGGTAT49400.027.1181661
CATCTCC444050.025.6226439
TGCGCGA5650.024.93938394
ACGCAGA344500.024.2899957
Produced by FastQC (version 0.11.5)