##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140086_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6724245
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.943697173437315	18.0	18.0	30.0	18.0	33.0
2	31.170164977629458	32.0	31.0	33.0	27.0	33.0
3	31.87317163488243	33.0	31.0	33.0	29.0	33.0
4	32.62879936706649	33.0	33.0	33.0	31.0	34.0
5	33.005067780843795	33.0	33.0	34.0	32.0	34.0
6	37.00070625029279	38.0	37.0	38.0	36.0	38.0
7	37.228282431707946	38.0	38.0	38.0	36.0	38.0
8	37.17292841055018	38.0	38.0	38.0	36.0	38.0
9	37.403192477371064	38.0	38.0	38.0	37.0	38.0
10-11	37.45606465856018	38.0	38.0	38.0	37.0	38.0
12-13	37.488180903581	38.0	38.0	38.0	37.0	38.0
14-15	37.45430944291887	38.0	38.0	38.0	37.0	38.0
16-17	37.45203141765358	38.0	38.0	38.0	37.0	38.0
18-19	37.42207452286465	38.0	38.0	38.0	37.0	38.0
20-21	37.51227762992238	38.0	38.0	38.0	37.0	38.0
22-23	37.52915869201479	38.0	38.0	38.0	37.5	38.0
24-25	37.533441600311434	38.0	38.0	38.0	38.0	38.0
26-27	37.50708363065883	38.0	38.0	38.0	37.5	38.0
28-29	37.414160387184104	38.0	38.0	38.0	37.0	38.0
30-31	37.460180146168085	38.0	38.0	38.0	37.0	38.0
32-33	37.43667114465076	38.0	38.0	38.0	37.0	38.0
34-35	37.38728755503543	38.0	38.0	38.0	37.0	38.0
36-37	37.36500490818363	38.0	38.0	38.0	37.0	38.0
38-39	37.277366186450905	38.0	38.0	38.0	37.0	38.0
40-41	37.3974849143125	38.0	38.0	38.0	37.0	38.0
42-43	37.3531312520777	38.0	38.0	38.0	37.0	38.0
44-45	37.355580566307665	38.0	38.0	38.0	37.0	38.0
46-47	37.3254380246054	38.0	38.0	38.0	37.0	38.0
48-49	37.29114514828237	38.0	38.0	38.0	37.0	38.0
50-51	37.25861345366533	38.0	38.0	38.0	37.0	38.0
52-53	37.23602244575862	38.0	38.0	38.0	37.0	38.0
54-55	37.21504709852189	38.0	38.0	38.0	37.0	38.0
56-57	37.21313555803037	38.0	38.0	38.0	37.0	38.0
58-59	37.19888051375817	38.0	38.0	38.0	37.0	38.0
60-61	37.178349540755804	38.0	38.0	38.0	37.0	38.0
62-63	37.16817196299979	38.0	38.0	38.0	37.0	38.0
64-65	37.158477853872384	38.0	38.0	38.0	36.5	38.0
66-67	37.14336124872891	38.0	38.0	38.0	36.0	38.0
68-69	37.13591503519773	38.0	38.0	38.0	36.0	38.0
70-71	37.136665552913584	38.0	38.0	38.0	36.0	38.0
72-73	37.082791534182405	38.0	38.0	38.0	36.0	38.0
74-75	37.08761523584673	38.0	38.0	38.0	36.0	38.0
76-77	37.098425934403394	38.0	38.0	38.0	36.0	38.0
78-79	37.10500232808759	38.0	38.0	38.0	36.0	38.0
80-81	37.112348045275034	38.0	38.0	38.0	36.0	38.0
82-83	37.12462353854084	38.0	38.0	38.0	36.0	38.0
84-85	37.11828718179916	38.0	38.0	38.0	36.0	38.0
86-87	37.110152546896785	38.0	38.0	38.0	36.0	38.0
88-89	37.09581234104516	38.0	38.0	38.0	36.0	38.0
90-91	37.09117867580218	38.0	38.0	38.0	36.0	38.0
92-93	37.089457773888455	38.0	38.0	38.0	36.0	38.0
94-95	37.05258852249503	38.0	38.0	38.0	36.0	38.0
96-97	37.06292131591995	38.0	38.0	38.0	36.0	38.0
98-99	37.05981482242876	38.0	38.0	38.0	36.0	38.0
100	35.47542205034057	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	5.0
18	43.0
19	39.0
20	66.0
21	225.0
22	542.0
23	1286.0
24	2583.0
25	5078.0
26	8973.0
27	14348.0
28	22416.0
29	32897.0
30	45954.0
31	60960.0
32	79186.0
33	106288.0
34	162567.0
35	303665.0
36	1076126.0
37	4800998.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.617716933276526	20.022976557219437	19.118309936654597	24.240996572849443
2	13.265553628033524	20.91834981971927	43.684731422979716	22.131365129267493
3	16.819955251481762	27.901526491078183	32.45653601259323	22.82198224484682
4	11.211340455322494	19.70085860940522	37.767095041896894	31.32070589337539
5	11.90114149872572	38.63519276027575	36.033744053950514	13.429921687048024
6	27.244223789294157	38.51369780099336	19.221640345390348	15.020438064322125
7	24.131110630264065	33.84446878422782	22.32313070091884	19.701289884589272
8	23.79161844561811	36.45326863607824	22.19442726778721	17.560685650516444
9	24.51649496923961	16.643833823699122	20.503397265293643	38.33627394176762
10-11	23.3057168499958	27.01349370821557	28.80952434065088	20.871265101137748
12-13	24.6493100712422	24.330590274447168	28.56650077443639	22.453598879874246
14-15	21.945454243967482	25.89199426047045	25.84137890881273	26.321172586749338
16-17	21.483913807423733	29.90540201911144	26.309169282201943	22.301514891262887
18-19	21.493966980679616	27.62358450651337	28.476780664595058	22.405667848211955
20-21	22.484952177670298	26.60512708215283	28.22555823214549	22.68436250803138
22-23	22.072164581773514	28.21592852713338	28.032857660112786	21.679049230980326
24-25	22.2593066640943	28.62148294497758	28.231318492212637	20.887891898715484
26-27	22.110977394276937	28.8523906378427	28.263970383313787	20.772661584566574
28-29	21.252838989756242	27.592432380561267	28.604179636639117	22.550548993043375
30-31	23.011923723093833	27.804231412531564	28.69981460940563	20.484030254968964
32-33	22.775889703248374	27.69400408638441	29.189169000542346	20.340937209824865
34-35	21.93050324747529	27.978377512540913	29.794601545661198	20.2965176943226
36-37	21.94536247437433	27.297959366200413	29.31507031436546	21.441607845059796
38-39	21.109030275723462	26.435813215729166	30.133443657029535	22.321712851517837
40-41	21.04073244235475	26.350898049375342	30.61643197654253	21.991937531727377
42-43	21.942802637023895	26.005780414856737	30.661357576673666	21.390059371445705
44-45	20.800113846235647	27.10581913773987	30.338841114129288	21.755225901895194
46-47	22.221421168267103	25.921484116878723	28.99898584988254	22.858108864971634
48-49	21.722900675453808	25.99879026776893	29.427443756201793	22.850865300575464
50-51	20.995277402958866	26.619215683643155	30.012570255472315	22.372936657925667
52-53	21.07247219094907	27.272782973600627	30.636256445602942	21.018488389847352
54-55	21.000850772353623	27.72637763480671	29.092623760842322	22.180147831997346
56-57	22.035952136335897	28.796745419868213	27.517698222335547	21.649604221460343
58-59	20.359139979453577	30.28124144196484	29.154228254077267	20.205390324504318
60-61	21.429356974325735	31.870236673471048	26.567148918159127	20.13325743404409
62-63	20.193631867827456	32.73614870585019	26.684566010244254	20.3856534160781
64-65	20.256311667586953	32.363559622042395	26.863342406537544	20.51678630383311
66-67	20.484166771606745	31.967551576116755	26.84937715535924	20.698904496917258
68-69	20.74664190882114	31.184357931945762	27.166515803862374	20.902484355370728
70-71	20.922187025912674	30.16818585938747	27.557227981090954	21.352399133608905
72-73	21.450671935464925	28.66614286800948	28.18251494607474	21.700670250450862
74-75	21.52232857348325	28.549726800820473	28.119259529114544	21.808685096581737
76-77	21.58669443269964	28.496959303267516	27.951935653404043	21.964410610628807
78-79	21.650928765706272	28.51348176340907	27.988218253964682	21.847371216919974
80-81	21.616785272482602	28.42444461762203	28.000582903595628	21.958187206299737
82-83	21.7383426741952	28.27271319974854	28.14125403380285	21.847690092253416
84-85	21.92763713818855	28.23647537990144	27.9661528516076	21.86973463030241
86-87	21.690434487099385	28.276643928938167	28.086495757626484	21.946425826335965
88-89	21.908118705276504	28.174178275982058	27.98956641661332	21.928136602128117
90-91	21.917146771788055	28.15790910470079	28.026075766727715	21.89886835678344
92-93	21.975706680416927	28.17545838426917	27.987131976169078	21.86170295914482
94-95	21.844662129743135	28.294294259703356	27.98556873766992	21.87547487288359
96-97	21.874611095637842	28.38154630570561	28.117195516533126	21.626647082123423
98-99	22.92418814291094	30.20649193663679	28.377137510623296	18.492182409828974
100	28.951887131504822	41.285419446176576	29.76269342231861	0.0
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2273.0
1	2135.5
2	2380.5
3	3007.5
4	3933.0
5	5104.0
6	5727.5
7	6002.5
8	6050.0
9	6020.0
10	6529.0
11	8271.0
12	10861.0
13	17063.0
14	26477.0
15	33814.0
16	38506.0
17	39314.5
18	36996.5
19	33242.0
20	29673.5
21	27828.5
22	28499.5
23	33102.0
24	40842.0
25	51677.5
26	66214.0
27	81636.5
28	96485.0
29	113113.5
30	129344.5
31	146002.0
32	164217.0
33	181612.5
34	196354.0
35	209897.0
36	223572.0
37	235309.5
38	244127.0
39	250752.5
40	258991.0
41	264991.5
42	267750.0
43	273092.5
44	335777.0
45	415561.5
46	389525.0
47	361457.0
48	331531.5
49	270159.5
50	251545.0
51	240597.0
52	229208.0
53	215411.0
54	200078.5
55	183222.5
56	164548.5
57	145047.5
58	126730.5
59	109713.0
60	92225.0
61	74349.0
62	57562.5
63	43678.5
64	32953.5
65	24620.0
66	18048.0
67	13375.0
68	9829.5
69	6947.5
70	4920.0
71	3475.0
72	2418.0
73	1605.5
74	1062.0
75	739.0
76	507.0
77	313.5
78	212.0
79	150.5
80	114.0
81	84.0
82	59.0
83	43.0
84	31.0
85	22.5
86	14.0
87	10.0
88	9.0
89	7.0
90	4.5
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.4871558070831745E-4
3	0.0
4	0.0
5	0.00673681580608678
6	4.015320679124571E-4
7	0.0
8	4.461467421249523E-5
9	1.9333025492081267E-4
10-11	0.0
12-13	0.0
14-15	7.4357790354158715E-6
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.0420451833768784E-4
26-27	7.445718108984167E-6
28-29	7.152370220987378E-4
30-31	2.686272275633026E-4
32-33	0.0016166839387390555
34-35	2.8549812284984225E-4
36-37	0.0013143617055429429
38-39	7.537240247857958E-4
40-41	6.603627618367145E-4
42-43	8.517911968618772E-5
44-45	5.473373717969719E-5
46-47	4.743060132279204E-4
48-49	0.0015662822269560527
50-51	0.0018997709927119934
52-53	0.001878940823085549
54-55	0.0013926629735002607
56-57	0.0031600742251539577
58-59	3.947020570527547E-4
60-61	3.396953459274817E-5
62-63	0.002062478247973917
64-65	0.0024616275194889064
66-67	0.0033353779616417264
68-69	7.747400473703409E-4
70-71	0.005230070425089123
72-73	0.0011457964339541271
74-75	0.004579229357683508
76-77	3.062420728992461E-4
78-79	0.0012002449699983766
80-81	0.0
82-83	0.0
84-85	9.974134782769059E-4
86-87	0.0
88-89	1.0647816340500982E-5
90-91	0.0034203420147799774
92-93	6.230867411076777E-4
94-95	1.1075751160212624E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1086.0
20-21	3175.0
22-23	1962.0
24-25	2115.0
26-27	3358.0
28-29	7165.0
30-31	19628.0
32-33	23726.0
34-35	31549.0
36-37	48742.0
38-39	56751.0
40-41	54199.0
42-43	59342.0
44-45	68877.0
46-47	68984.0
48-49	64705.0
50-51	75386.0
52-53	52455.0
54-55	55045.0
56-57	57104.0
58-59	61084.0
60-61	94679.0
62-63	85778.0
64-65	87908.0
66-67	113423.0
68-69	129648.0
70-71	178269.0
72-73	82617.0
74-75	59077.0
76-77	61305.0
78-79	64714.0
80-81	64455.0
82-83	59538.0
84-85	56413.0
86-87	59264.0
88-89	60753.0
90-91	61228.0
92-93	59482.0
94-95	63094.0
96-97	180378.0
98-99	1355861.0
100-101	2929923.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.82471511461229
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.01081854164444	74.61306506371794
2	7.395122835079748	12.397881297762478
3	1.9942883438953154	5.015119568502502
4	0.7420526107583026	2.4880939478747597
5	0.35456828519418165	1.4860792747539442
6	0.1807361953673418	0.9090096052539801
7	0.10582273106723193	0.6209392199052663
8	0.06013023482878624	0.40323198434381946
9	0.03847957648332601	0.2902985582801164
>10	0.11478099343217257	1.4003004217194523
>50	0.0018497340792414723	0.1059373521097726
>100	0.001261808709368393	0.2147736813429736
>500	8.81094605518914E-5	0.05527002443297926
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.974311614166349E-5
2	0.0	0.0	0.0	2.974311614166349E-5	2.974311614166349E-5
3	0.0	0.0	0.0	5.948623228332698E-5	2.974311614166349E-5
4	0.0	1.4871558070831745E-5	0.0	7.435779035415871E-5	2.974311614166349E-5
5	0.0	1.4871558070831745E-5	0.0	7.435779035415871E-5	2.974311614166349E-5
6	0.0	1.4871558070831745E-5	0.0	7.435779035415871E-5	2.974311614166349E-5
7	0.0	1.4871558070831745E-5	0.0	8.922934842499046E-5	2.974311614166349E-5
8	0.0	2.974311614166349E-5	0.0	8.922934842499046E-5	2.974311614166349E-5
9	0.0	7.435779035415871E-5	0.0	1.1897246456665396E-4	2.974311614166349E-5
10-11	0.0	7.435779035415871E-5	0.0	1.1897246456665396E-4	2.974311614166349E-5
12-13	0.0	7.435779035415871E-5	0.0	1.4127980167290158E-4	3.7178895177079356E-5
14-15	0.0	1.2640824360206983E-4	0.0	1.561513597437333E-4	7.435779035415871E-5
16-17	0.0	1.4871558070831743E-4	0.0	1.7102291781456505E-4	8.922934842499046E-5
18-19	0.0	1.7845869684998092E-4	0.0	1.9333025492081267E-4	1.338440226374857E-4
20-21	0.0	2.0820181299164442E-4	0.0	2.156375920270603E-4	1.338440226374857E-4
22-23	0.0	2.5281648720413966E-4	0.0	3.0486694045205075E-4	1.4871558070831743E-4
24-25	7.435779035415872E-6	2.8255960334580316E-4	0.0	3.717889517707936E-4	1.6358713877914917E-4
26-27	1.4871558070831745E-5	2.9743116141663485E-4	0.0	4.833256373020317E-4	1.6358713877914917E-4
28-29	1.4871558070831745E-5	2.9743116141663485E-4	0.0	9.517797165332317E-4	1.6358713877914917E-4
30-31	1.4871558070831745E-5	3.2717427755829835E-4	0.0	0.0025207290930059807	1.6358713877914917E-4
32-33	1.4871558070831745E-5	3.2717427755829835E-4	0.0	0.006298104842997244	1.6358713877914917E-4
34-35	1.4871558070831745E-5	3.6435317273537775E-4	0.0	0.010856237391707173	1.6358713877914917E-4
36-37	1.4871558070831745E-5	4.015320679124571E-4	0.0	0.017972277928600164	1.6358713877914917E-4
38-39	1.4871558070831745E-5	4.015320679124571E-4	0.0	0.035498409115075374	1.7102291781456505E-4
40-41	1.4871558070831745E-5	4.7588985826661583E-4	0.0	0.053611966845348434	1.9333025492081267E-4
42-43	1.4871558070831745E-5	4.7588985826661583E-4	0.0	0.06476563539847224	2.0820181299164442E-4
44-45	1.4871558070831745E-5	4.833256373020317E-4	0.0	0.077205693724723	2.0820181299164442E-4
46-47	1.4871558070831745E-5	4.907614163374475E-4	0.0	0.09101393539349027	2.0820181299164442E-4
48-49	1.4871558070831745E-5	4.907614163374475E-4	0.0	0.10466602570251382	2.0820181299164442E-4
50-51	1.4871558070831745E-5	5.20504532479111E-4	0.0	0.11790171238555405	2.0820181299164442E-4
52-53	1.4871558070831745E-5	5.20504532479111E-4	0.0	0.151318103370713	2.2307337106247617E-4
54-55	1.4871558070831745E-5	5.279403115145269E-4	0.0	0.19042286531796507	2.3794492913330791E-4
56-57	1.4871558070831745E-5	5.353760905499428E-4	0.0	0.2460945429561237	2.3794492913330791E-4
58-59	1.4871558070831745E-5	5.353760905499428E-4	0.0	0.2874226028349651	2.3794492913330791E-4
60-61	1.4871558070831745E-5	5.353760905499428E-4	0.0	0.30639127515431097	2.3794492913330791E-4
62-63	1.4871558070831745E-5	5.353760905499428E-4	0.0	0.32213281937228644	2.3794492913330791E-4
64-65	1.4871558070831745E-5	6.692201131874285E-4	0.0	0.3304237129967751	2.3794492913330791E-4
66-67	1.4871558070831745E-5	6.692201131874285E-4	0.0	0.33805282228711175	2.3794492913330791E-4
68-69	1.4871558070831745E-5	6.692201131874285E-4	0.0	0.3452283490562881	2.3794492913330791E-4
70-71	1.4871558070831745E-5	6.692201131874285E-4	0.0	0.3510951787152312	2.3794492913330791E-4
72-73	1.4871558070831745E-5	6.766558922228444E-4	0.0	0.35502870582496626	2.5281648720413966E-4
74-75	1.4871558070831745E-5	6.989632293290919E-4	0.0	0.35835993483283257	2.5281648720413966E-4
76-77	1.4871558070831745E-5	7.58449461612419E-4	0.0	0.36164654916648636	2.5281648720413966E-4
78-79	1.4871558070831745E-5	7.58449461612419E-4	0.0	0.3635426728205174	2.5281648720413966E-4
80-81	1.4871558070831745E-5	7.58449461612419E-4	0.0	0.36422676449177566	2.6025226623955556E-4
82-83	1.4871558070831745E-5	7.58449461612419E-4	0.0	0.3647323974661839	3.123027194874666E-4
84-85	1.4871558070831745E-5	7.58449461612419E-4	0.0	0.36496290661628183	3.123027194874666E-4
86-87	1.4871558070831745E-5	7.58449461612419E-4	0.0	0.3650075212904943	3.1973849852288245E-4
88	1.4871558070831745E-5	7.881925777540825E-4	0.0	0.3650075212904943	3.2717427755829835E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	18695	0.0	58.8553	1
TATACAC	23775	0.0	48.58238	3
GGTATCA	11500	0.0	48.549427	1
TTATACA	23680	0.0	48.259125	2
GTATCAA	21770	0.0	34.53892	1
TACACAT	34885	0.0	33.168644	5
ATACACA	36955	0.0	31.8965	4
ACACATC	37830	0.0	30.205725	6
ATCAACG	28285	0.0	29.515123	3
TCAACGC	28880	0.0	28.977749	4
TATCAAC	29160	0.0	28.937181	2
CAACGCA	29200	0.0	28.6462	5
TGGTATC	4905	0.0	28.56068	2
ACATCTC	40280	0.0	28.358135	8
CACATCT	40585	0.0	28.175209	7
AACGCAG	30110	0.0	27.85913	6
GTGGTAT	4940	0.0	27.118166	1
CATCTCC	44405	0.0	25.622643	9
TGCGCGA	565	0.0	24.939383	94
ACGCAGA	34450	0.0	24.289995	7
>>END_MODULE