FastQCFastQC Report
Fri 27 May 2016
SRR2140085_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140085_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4889337
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84250.054.2683331
CTTATAC55500.044.900291
GTATCAA154900.037.4653471
TATACAC77050.035.2328153
TTATACA87700.030.331342
ATCAACG188450.030.3270553
TCAACGC190200.030.0701164
TATCAAC195150.029.5657882
CAACGCA193700.029.5050775
AACGCAG199850.028.7221056
ACACATC97350.026.5034316
ACATCTC98600.026.1248198
TACACAT105850.025.9244655
ACGCAGA225700.025.3766357
CGCAGAG229350.024.9727768
GTGGTAT33200.024.935361
TGGTATC33200.024.5556342
CACATCT104900.024.39567
CATCTCC108650.023.437579
ATACACA134400.022.1996734
Produced by FastQC (version 0.11.5)