##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2140085_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4889337 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.419834631975665 32.0 18.0 33.0 18.0 33.0 2 31.044808120201164 32.0 31.0 33.0 27.0 33.0 3 32.35599489255905 33.0 33.0 33.0 30.0 34.0 4 32.691146468324845 33.0 33.0 34.0 31.0 34.0 5 33.09241600650559 33.0 33.0 34.0 33.0 34.0 6 37.01064295629448 38.0 37.0 38.0 35.0 38.0 7 37.35307097874415 38.0 38.0 38.0 37.0 38.0 8 37.31270088357583 38.0 38.0 38.0 36.0 38.0 9 37.46721610721454 38.0 38.0 38.0 37.0 38.0 10-11 37.4993506277027 38.0 38.0 38.0 37.0 38.0 12-13 37.518914834465285 38.0 38.0 38.0 37.0 38.0 14-15 37.47817137579185 38.0 38.0 38.0 37.0 38.0 16-17 37.49312831167089 38.0 38.0 38.0 37.0 38.0 18-19 37.41991419286501 38.0 38.0 38.0 37.0 38.0 20-21 37.50904987173736 38.0 38.0 38.0 37.0 38.0 22-23 37.523047780241455 38.0 38.0 38.0 37.0 38.0 24-25 37.528373136311885 38.0 38.0 38.0 37.0 38.0 26-27 37.511062170871725 38.0 38.0 38.0 37.0 38.0 28-29 37.44899637190362 38.0 38.0 38.0 37.0 38.0 30-31 37.48567838012683 38.0 38.0 38.0 37.0 38.0 32-33 37.46895960668661 38.0 38.0 38.0 37.0 38.0 34-35 37.43828575497155 38.0 38.0 38.0 37.0 38.0 36-37 37.414409290105155 38.0 38.0 38.0 37.0 38.0 38-39 37.3783489567775 38.0 38.0 38.0 37.0 38.0 40-41 37.3847930608906 38.0 38.0 38.0 37.0 38.0 42-43 37.31418853328309 38.0 38.0 38.0 37.0 38.0 44-45 37.3110586725404 38.0 38.0 38.0 37.0 38.0 46-47 37.273356985935195 38.0 38.0 38.0 37.0 38.0 48-49 37.224264186159985 38.0 38.0 38.0 37.0 38.0 50-51 37.18606001711528 38.0 38.0 38.0 37.0 38.0 52-53 37.16466121999974 38.0 38.0 38.0 37.0 38.0 54-55 37.1523158633704 38.0 38.0 38.0 37.0 38.0 56-57 37.15447473348224 38.0 38.0 38.0 37.0 38.0 58-59 37.149652135771916 38.0 38.0 38.0 37.0 38.0 60-61 37.147315999794415 38.0 38.0 38.0 36.5 38.0 62-63 37.15255534095512 38.0 38.0 38.0 36.5 38.0 64-65 37.154700525073665 38.0 38.0 38.0 36.0 38.0 66-67 37.155148432679695 38.0 38.0 38.0 36.0 38.0 68-69 37.15888929310793 38.0 38.0 38.0 36.0 38.0 70-71 37.167726188691105 38.0 38.0 38.0 36.0 38.0 72-73 37.138629220502295 38.0 38.0 38.0 36.0 38.0 74-75 37.13727491038787 38.0 38.0 38.0 36.0 38.0 76-77 37.142726850300136 38.0 38.0 38.0 36.0 38.0 78-79 37.12936235640352 38.0 38.0 38.0 36.0 38.0 80-81 37.12329288258105 38.0 38.0 38.0 36.0 38.0 82-83 37.129655461938285 38.0 38.0 38.0 36.0 38.0 84-85 37.11415979760888 38.0 38.0 38.0 36.0 38.0 86-87 37.100221862605835 38.0 38.0 38.0 36.0 38.0 88-89 37.08486313908827 38.0 38.0 38.0 36.0 38.0 90-91 37.07353554496975 38.0 38.0 38.0 36.0 38.0 92-93 37.07008732640189 38.0 38.0 38.0 36.0 38.0 94-95 37.031274962126425 38.0 38.0 38.0 36.0 38.0 96-97 37.046005758171404 38.0 38.0 38.0 36.0 38.0 98-99 37.0397061898602 38.0 38.0 38.0 36.0 38.0 100 35.373429221054394 38.0 35.0 38.0 29.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 2.0 19 18.0 20 57.0 21 147.0 22 396.0 23 841.0 24 1768.0 25 3346.0 26 5741.0 27 9713.0 28 15919.0 29 24466.0 30 34180.0 31 44349.0 32 56110.0 33 73870.0 34 107718.0 35 197748.0 36 655385.0 37 3657563.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.29337495042784 19.430671275062448 14.433102074984808 22.842851699524903 2 13.936618692793775 20.81611215283573 43.665284881685174 21.581984272685325 3 17.330447870539505 27.414146335177957 31.32506513664327 23.930340657639267 4 11.6752639468296 19.23857979108415 39.60784458097284 29.47831168111341 5 12.516311875700032 38.26318101825375 36.142575815961955 13.07793129008426 6 28.070941518897403 38.899966314354764 19.488812663839145 13.540279502908684 7 25.12017478034343 33.79108455809039 23.468212561335005 17.620528100231176 8 23.613282220703724 36.93888307464434 21.98721175260511 17.460622952046833 9 25.25071942372472 17.06902581744329 21.819267670621535 35.86098708821045 10-11 23.410791688116404 27.369825397594806 29.443797799169907 19.77558511511888 12-13 24.578076741284146 24.93650979672704 29.467911498021103 21.01750196396771 14-15 21.765139118044022 26.205373039330283 27.405331234071205 24.624156608554493 16-17 20.93538449078065 29.52458584875618 28.01362434211428 21.52640531834889 18-19 20.89791519790925 28.19366920300237 30.218187046628202 20.69022855246018 20-21 21.94509237496816 27.14008563011623 30.119632023867705 20.79518997104791 22-23 21.49124089845603 27.450741367147575 29.93873363900381 21.119284095392587 24-25 21.3787438919847 27.674737459405186 30.10411623316096 20.842402415449154 26-27 21.366182315842018 27.835784408990392 30.047153117731412 20.75088015743618 28-29 21.04940736800218 27.865302063409374 30.089663491521673 20.995627077066768 30-31 21.925156093391248 27.515878222827254 29.938730367008326 20.62023531677317 32-33 20.9215396067551 27.99673252367786 30.011330632200412 21.070397237366635 34-35 21.328672444792762 27.900263740142695 30.169522521501158 20.601541293563383 36-37 21.080459675008818 27.911190589714924 30.11554966246301 20.89280007281325 38-39 20.937937317344215 27.46665198272736 30.46114508911749 21.13426561081093 40-41 21.2692110145596 27.37942998501064 30.361337931468224 20.990021068961543 42-43 21.561390883644243 27.291051136123684 30.127570024781704 21.019987955450368 44-45 21.240168284759317 27.77489772928884 29.916283593002717 21.06865039294913 46-47 21.629879131176274 27.413149464492687 29.374180949586503 21.582790454744533 48-49 21.459921969331983 27.490868087107994 29.459504820835264 21.589705122724762 50-51 21.177564802015283 27.68590012349924 29.56190662475177 21.574628449733705 52-53 21.335094065777977 27.67540076808838 29.772730816618378 21.21677434951527 54-55 21.412733795125803 27.662682900683162 29.33957251791568 21.585010786275355 56-57 21.760088964036495 27.93792560477033 28.867111546903175 21.434873884290003 58-59 21.198587107257076 28.30281074334226 29.52444200525986 20.974160144140804 60-61 21.576603999984844 28.72516110574918 28.75298932344712 20.945245570818855 62-63 21.200520729796015 29.077561546299968 28.64418416197381 21.077733561930206 64-65 21.188646616453322 29.015770019283703 28.698777293701244 21.096806070561733 66-67 21.367152425199425 28.93959567768883 28.525109633276397 21.168142263835353 68-69 21.38747667221844 28.73454556977401 28.691118806885097 21.18685895112245 70-71 21.39988306985919 28.238300414074846 28.88955797291232 21.472258543153647 72-73 21.53660250084523 27.93013794494944 29.045385421100807 21.487874133104523 74-75 21.496161902306312 28.058302504824912 28.917822712275722 21.527712880593057 76-77 21.568543617009432 28.046822899853368 28.824413240084564 21.560220243052637 78-79 21.565410735296638 28.047884031173943 28.757818376900218 21.628886856629197 80-81 21.562882951302647 28.00941605293007 28.734645360705645 21.69305563506164 82-83 21.674885046873143 27.9671065574168 28.835747601295907 21.52226079441415 84-85 21.89375452706909 27.92546760713047 28.65960841581481 21.52116944998563 86-87 21.51969707494836 28.074141861209238 28.727832258310233 21.678328805532168 88-89 21.777329764733878 28.00958727401634 28.569921821392025 21.64316113985776 90-91 21.740619823634127 28.036359137553006 28.65739245187054 21.565628586942324 92-93 21.78377865998207 28.040051907798098 28.571782518964678 21.604386913255155 94-95 21.70231225811618 28.146729539718113 28.53647873410251 21.614479468063195 96-97 21.71900446233176 28.24575980964389 28.662020388249825 21.373215339774525 98-99 22.794737983151634 30.109653741328046 28.82656095143707 18.26904732408325 100 21.671513655701396 31.078527756894502 22.796204613183484 24.453753974220618 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 989.0 1 939.5 2 1499.0 3 2364.0 4 3003.5 5 3934.5 6 4826.0 7 6265.5 8 8496.5 9 10026.0 10 10212.5 11 10207.0 12 11133.0 13 13252.5 14 17120.5 15 21323.0 16 24197.0 17 25761.0 18 25468.5 19 23405.0 20 21019.5 21 19543.5 22 20157.5 23 23525.0 24 29419.0 25 38049.0 26 49657.0 27 61904.0 28 73315.5 29 85996.0 30 98225.5 31 109709.0 32 122591.0 33 134653.0 34 147013.5 35 159449.0 36 171573.5 37 182192.0 38 186461.0 39 188451.5 40 193770.0 41 196778.0 42 198944.0 43 216591.0 44 224876.5 45 216852.5 46 210080.5 47 201749.0 48 195510.0 49 188494.5 50 181593.5 51 173292.5 52 162910.5 53 151877.5 54 140347.5 55 128543.5 56 116666.5 57 103649.0 58 90710.5 59 79275.5 60 67160.0 61 54660.5 62 42702.5 63 32539.0 64 24609.5 65 18044.5 66 13207.5 67 10080.5 68 7646.0 69 5662.0 70 4185.0 71 3022.0 72 2145.0 73 1547.5 74 1119.5 75 747.5 76 441.5 77 268.5 78 192.0 79 121.5 80 70.0 81 38.5 82 26.5 83 23.5 84 16.0 85 13.0 86 11.0 87 8.5 88 6.0 89 3.0 90 2.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 1.8407403703201477E-4 3 0.0 4 0.0 5 0.005338147073928429 6 2.8633739093868963E-4 7 0.0 8 8.18106831253399E-5 9 1.4316869546934482E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 7.159123694623283E-5 26-27 1.0228440977957606E-5 28-29 5.423021236755806E-4 30-31 1.7413390405549668E-4 32-33 0.0014372254321967858 34-35 3.0891265525563914E-4 36-37 0.00137557229754601 38-39 7.494582041732331E-4 40-41 8.804861625310372E-4 42-43 1.5836952868911525E-4 44-45 1.3844092305165967E-4 46-47 4.087168549453126E-4 48-49 0.001455653252097743 50-51 0.0016994013392817497 52-53 0.0017804594027609286 54-55 0.0015393588971765147 56-57 0.003085180481932215 58-59 5.798525128079188E-4 60-61 3.444085162582632E-5 62-63 0.0019125361295461564 64-65 0.002446991891792848 66-67 0.0033522611712326717 68-69 8.402011777700054E-4 70-71 0.004940808866391036 72-73 9.525383538949665E-4 74-75 0.0037154212499577793 76-77 1.7709274929156576E-4 78-79 0.0011005819262947963 80-81 0.0 82-83 1.310641755735696E-5 84-85 9.279411292937493E-4 86-87 0.0 88-89 1.3574322810970769E-5 90-91 0.0029553813026083646 92-93 4.4541514013386675E-4 94-95 1.4098359176565593E-5 96-97 1.4340953590236219E-5 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 29.0 20-21 114.0 22-23 266.0 24-25 412.0 26-27 1248.0 28-29 3531.0 30-31 10182.0 32-33 13618.0 34-35 18968.0 36-37 28793.0 38-39 33898.0 40-41 33597.0 42-43 38402.0 44-45 45669.0 46-47 46773.0 48-49 43399.0 50-51 39695.0 52-53 38211.0 54-55 41231.0 56-57 42903.0 58-59 42467.0 60-61 41995.0 62-63 42468.0 64-65 46594.0 66-67 53359.0 68-69 57892.0 70-71 69402.0 72-73 46572.0 74-75 43808.0 76-77 45210.0 78-79 46367.0 80-81 46369.0 82-83 43521.0 84-85 42888.0 86-87 44766.0 88-89 45797.0 90-91 45486.0 92-93 44937.0 94-95 50741.0 96-97 141417.0 98-99 305679.0 100-101 3060663.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.18937345939486 #Duplication Level Percentage of deduplicated Percentage of total 1 90.03930872036545 77.60431605325326 2 7.128611016639745 12.288210343198386 3 1.6958040877221803 4.3848087549196615 4 0.5712653025757236 1.9694799403237297 5 0.2409893811732003 1.0385361886842712 6 0.12482585470587973 0.6455197325179938 7 0.06013156181488655 0.3627891146571961 8 0.039052870665742395 0.2692753963576894 9 0.028182366372072352 0.21861184501908312 >10 0.06952232583833623 0.8760228962641824 >50 0.0012865034674021175 0.07579004338759256 >100 8.46192759968252E-4 0.14664121411609807 >500 1.390527205922281E-4 0.07960947752460401 >1k 3.4763178804399176E-5 0.04038899977619692 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.0226335390667487E-4 0.0 0.0 0.0 3 0.0 1.0226335390667487E-4 0.0 0.0 0.0 4 0.0 1.4316869546934482E-4 0.0 0.0 0.0 5 0.0 1.636213662506798E-4 0.0 0.0 0.0 6 0.0 1.636213662506798E-4 0.0 0.0 0.0 7 0.0 1.636213662506798E-4 0.0 0.0 0.0 8 0.0 2.0452670781334975E-4 0.0 2.0452670781334974E-5 0.0 9 0.0 3.272427325013596E-4 0.0 4.090534156266995E-5 0.0 10-11 4.090534156266995E-5 3.272427325013596E-4 0.0 4.090534156266995E-5 0.0 12-13 4.090534156266995E-5 3.272427325013596E-4 0.0 4.090534156266995E-5 0.0 14-15 6.135801234400492E-5 3.886007448453645E-4 0.0 4.090534156266995E-5 0.0 16-17 1.2271602468800984E-4 3.886007448453645E-4 0.0 4.090534156266995E-5 0.0 18-19 1.636213662506798E-4 4.0905341562669944E-4 0.0 4.090534156266995E-5 0.0 20-21 1.636213662506798E-4 4.3973242179870195E-4 0.0 4.090534156266995E-5 0.0 22-23 1.9430037242268227E-4 4.6018509258003695E-4 0.0 4.090534156266995E-5 0.0 24-25 2.1475304320401722E-4 4.8063776336137185E-4 0.0 8.18106831253399E-5 0.0 26-27 2.352057139853522E-4 4.908640987520394E-4 0.0 9.203701851600738E-5 0.0 28-29 2.454320493760197E-4 4.908640987520394E-4 0.0 3.9882708023603205E-4 0.0 30-31 2.9656372632935713E-4 5.931274526587143E-4 0.0 0.0017282506810228052 0.0 32-33 3.6814807406402954E-4 5.931274526587143E-4 0.0 0.004499587571893695 0.0 34-35 3.7837440945469704E-4 6.851644711747217E-4 0.0 0.007966315269329973 0.0 36-37 3.886007448453645E-4 7.874278250813965E-4 0.0 0.012834050915287696 0.0 38-39 3.886007448453645E-4 8.18106831253399E-4 0.0 0.02040153910438164 0.0 40-41 4.2950608640803445E-4 9.203701851600739E-4 0.0 0.028265591019804935 0.0 42-43 4.2950608640803445E-4 9.408228559414089E-4 0.0 0.035679684178038865 0.0 44-45 4.3973242179870195E-4 0.0010021808682854137 0.0 0.04465840665104492 0.0 46-47 4.4995875718936945E-4 0.0010430862098480837 0.0 0.05481315769397774 0.0 48-49 4.4995875718936945E-4 0.0010737652160200863 0.0 0.06469179768136252 0.0 50-51 4.4995875718936945E-4 0.001237386582270766 0.0 0.07422274226546462 0.0 52-53 4.704114279707044E-4 0.0012476129176614335 0.0 0.08359006548331605 0.0 54-55 4.704114279707044E-4 0.001257839253052101 0.0 0.09277331466413544 0.0 56-57 4.704114279707044E-4 0.0012680655884427683 0.0 0.09928749030799064 0.0 58-59 4.704114279707044E-4 0.0012680655884427683 0.0 0.1054948758901258 1.0226335390667487E-5 60-61 5.113167695333744E-4 0.0012885182592241033 0.0 0.11186588283851165 2.0452670781334974E-5 62-63 5.113167695333744E-4 0.0012885182592241033 0.0 0.11805281574986547 2.0452670781334974E-5 64-65 5.113167695333744E-4 0.0014930449670374532 0.0 0.12417839064887529 2.0452670781334974E-5 66-67 5.113167695333744E-4 0.0015032713024281208 0.0 0.12969038542444505 2.0452670781334974E-5 68-69 5.317694403147094E-4 0.0015134976378187882 0.0 0.13520238020001485 2.0452670781334974E-5 70-71 5.317694403147094E-4 0.001533950308600123 0.0 0.1401826055352699 2.0452670781334974E-5 72-73 5.317694403147094E-4 0.001554402979381458 0.0 0.14434472403927157 2.0452670781334974E-5 74-75 5.317694403147094E-4 0.001574855650162793 0.0 0.1476069250288945 2.0452670781334974E-5 76-77 5.522221110960444E-4 0.0016464399978974656 0.0 0.15081799434156412 2.0452670781334974E-5 78-79 5.726747818773793E-4 0.001656666333288133 0.0 0.15309846713368297 2.0452670781334974E-5 80-81 5.726747818773793E-4 0.001656666333288133 0.0 0.15370182092173235 2.0452670781334974E-5 82-83 5.931274526587143E-4 0.001656666333288133 0.0 0.15414155334353102 2.0452670781334974E-5 84-85 6.135801234400493E-4 0.001677119004069468 0.0 0.15434608005134437 2.0452670781334974E-5 86-87 6.135801234400493E-4 0.001677119004069468 0.0 0.15441766439907906 2.0452670781334974E-5 88 6.135801234400493E-4 0.001677119004069468 0.0 0.15441766439907906 2.0452670781334974E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8425 0.0 54.268333 1 CTTATAC 5550 0.0 44.90029 1 GTATCAA 15490 0.0 37.465347 1 TATACAC 7705 0.0 35.232815 3 TTATACA 8770 0.0 30.33134 2 ATCAACG 18845 0.0 30.327055 3 TCAACGC 19020 0.0 30.070116 4 TATCAAC 19515 0.0 29.565788 2 CAACGCA 19370 0.0 29.505077 5 AACGCAG 19985 0.0 28.722105 6 ACACATC 9735 0.0 26.503431 6 ACATCTC 9860 0.0 26.124819 8 TACACAT 10585 0.0 25.924465 5 ACGCAGA 22570 0.0 25.376635 7 CGCAGAG 22935 0.0 24.972776 8 GTGGTAT 3320 0.0 24.93536 1 TGGTATC 3320 0.0 24.555634 2 CACATCT 10490 0.0 24.3956 7 CATCTCC 10865 0.0 23.43757 9 ATACACA 13440 0.0 22.199673 4 >>END_MODULE