FastQCFastQC Report
Fri 27 May 2016
SRR2140084_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140084_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14856891
Sequences flagged as poor quality0
Sequence length19-100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA174300.048.684911
GTATCAA328150.032.321071
ATCAACG432800.028.8832443
TCAACGC437750.028.576094
CAACGCA443900.028.228145
AACGCAG457150.027.446776
TATCAAC466350.027.1070772
ACGCAGA524050.023.967147
CGCAGAG529000.023.7187278
TACATGG520250.021.000472
TGGTATC81350.020.7786542
ACATGGG517400.020.4986133
GCAGAGT624700.020.085179
GAGTACT350200.019.28816212-13
AGAGTAC585850.018.67727910-11
GTACATG511150.018.6254981
CAGAGTA626700.018.156210-11
CATGGGG303650.018.0741044
GTGGTAT86250.017.9197521
TGTATCA111600.017.1303651
Produced by FastQC (version 0.11.5)