##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140084_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14856891
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.2547372125164	18.0	18.0	28.0	18.0	33.0
2	30.135860053089168	31.0	27.0	33.0	27.0	33.0
3	31.919051637384968	33.0	31.0	33.0	29.0	33.0
4	32.33207142732621	33.0	33.0	33.0	31.0	34.0
5	32.85968383290959	33.0	33.0	34.0	32.0	34.0
6	36.926880529715135	38.0	37.0	38.0	35.0	38.0
7	37.15598263459024	38.0	38.0	38.0	36.0	38.0
8	37.203808522254086	38.0	38.0	38.0	36.0	38.0
9	37.31882087578081	38.0	38.0	38.0	37.0	38.0
10-11	37.35048510485808	38.0	38.0	38.0	37.0	38.0
12-13	37.363222291931734	38.0	38.0	38.0	37.0	38.0
14-15	37.364678956048074	38.0	38.0	38.0	37.0	38.0
16-17	37.36240691272488	38.0	38.0	38.0	37.0	38.0
18-19	37.36476871237731	38.0	38.0	38.0	37.0	38.0
20-21	37.32955331782168	38.0	38.0	38.0	37.0	38.0
22-23	37.342286876313096	38.0	38.0	38.0	37.0	38.0
24-25	37.36262171048031	38.0	38.0	38.0	37.0	38.0
26-27	37.336273648291936	38.0	38.0	38.0	37.0	38.0
28-29	37.31071317871519	38.0	38.0	38.0	37.0	38.0
30-31	37.30853363456069	38.0	38.0	38.0	37.0	38.0
32-33	37.247250991779296	38.0	38.0	38.0	37.0	38.0
34-35	37.256328706791514	38.0	38.0	38.0	37.0	38.0
36-37	37.25105305533631	38.0	38.0	38.0	37.0	38.0
38-39	37.24022677965981	38.0	38.0	38.0	37.0	38.0
40-41	37.23254888761268	38.0	38.0	38.0	37.0	38.0
42-43	37.203496899128396	38.0	38.0	38.0	37.0	38.0
44-45	37.173346637455495	38.0	38.0	38.0	36.5	38.0
46-47	37.13993057217775	38.0	38.0	38.0	36.0	38.0
48-49	37.093331092945675	38.0	38.0	38.0	36.0	38.0
50-51	37.05670495789069	38.0	38.0	38.0	36.0	38.0
52-53	37.0217347741327	38.0	38.0	38.0	36.0	38.0
54-55	36.99614311886835	38.0	38.0	38.0	36.0	38.0
56-57	36.96504401435068	38.0	38.0	38.0	36.0	38.0
58-59	36.97085492192507	38.0	38.0	38.0	36.0	38.0
60-61	36.99446397981298	38.0	38.0	38.0	36.0	38.0
62-63	37.00107841055604	38.0	38.0	38.0	36.0	38.0
64-65	36.99979451167256	38.0	38.0	38.0	36.0	38.0
66-67	37.00398233398313	38.0	38.0	38.0	36.0	38.0
68-69	37.0057013001983	38.0	38.0	38.0	36.0	38.0
70-71	36.99375321967744	38.0	38.0	38.0	36.0	38.0
72-73	36.97367012377939	38.0	38.0	38.0	36.0	38.0
74-75	36.969649107041775	38.0	38.0	38.0	36.0	38.0
76-77	36.96812469323127	38.0	38.0	38.0	36.0	38.0
78-79	36.939803230559015	38.0	38.0	38.0	36.0	38.0
80-81	36.91540204341034	38.0	38.0	38.0	35.5	38.0
82-83	36.91403099693508	38.0	38.0	38.0	36.0	38.0
84-85	36.86240339289259	38.0	38.0	38.0	35.0	38.0
86-87	36.8464147218548	38.0	38.0	38.0	35.0	38.0
88-89	36.8504934315616	38.0	38.0	38.0	35.0	38.0
90-91	36.82880493344228	38.0	38.0	38.0	35.0	38.0
92-93	36.83561893744235	38.0	38.0	38.0	35.0	38.0
94-95	36.78254188691756	38.0	38.0	38.0	35.0	38.0
96-97	36.75416603599958	38.0	38.0	38.0	35.0	38.0
98-99	36.828519278241195	38.0	38.0	38.0	35.0	38.0
100	35.14055554240868	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	7.0
18	24.0
19	81.0
20	205.0
21	581.0
22	1456.0
23	3587.0
24	8065.0
25	15590.0
26	27203.0
27	44026.0
28	68021.0
29	97135.0
30	132880.0
31	174141.0
32	226984.0
33	304501.0
34	444951.0
35	785350.0
36	2466940.0
37	1.0055163E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.709711473282	20.028773180068427	22.601269673446485	22.660245673203093
2	13.495709990367924	22.453890894356114	42.88443999001118	21.16595912526478
3	17.193476077868514	27.977044457013246	31.790951417762976	23.038528047355264
4	11.282515298792998	19.38331512292848	41.24347415620132	28.090695422077204
5	11.912448190650867	38.719596251902885	37.16870152591557	12.199254031530671
6	27.17448685197753	40.44888853798508	20.013639478509372	12.362985131528013
7	24.24490426698291	33.94453792519579	25.143699310979667	16.666858496841634
8	22.939826374172092	37.89316351583922	22.544427363706177	16.622582746282514
9	25.147758033628975	16.779735410322388	22.393669038831877	35.67883751721676
10-11	22.78750648436473	27.745889096177663	30.562195011055813	18.904409408401797
12-13	23.758355634432533	25.58061104439684	30.512070122881024	20.1489631982896
14-15	21.03501668013853	26.892073180048236	28.174037219496327	23.898872920316908
16-17	20.304503815771415	29.973151852564577	28.8438004963488	20.87854383531521
18-19	20.334176914941356	29.091446521348242	30.623126332420426	19.95125023128998
20-21	21.397801980044072	28.205896744358828	30.353696898626037	20.04260437697106
22-23	21.057737028200325	28.155853469397655	30.217769269372745	20.56864023302927
24-25	20.693934497617587	28.28654096478787	30.377749850608943	20.6417746869856
26-27	20.695161345592233	28.4415546907299	30.23548894122545	20.62779502245241
28-29	20.62894475631492	28.47659873757023	30.30721719333679	20.587239312778067
30-31	20.896358679354744	28.35016469410519	30.218569422968393	20.534907203571674
32-33	20.754063623951545	28.40907839999849	30.148491184583182	20.688366791466787
34-35	20.81439081805259	28.45764918301452	30.153707076929575	20.574252922003307
36-37	20.762675979850613	28.3851164413067	30.1825553700948	20.669652208747884
38-39	20.631773072349922	28.469090776623418	30.280376207926295	20.618759943100365
40-41	20.856962861937326	28.37392703672653	30.16848346042173	20.600626640914413
42-43	20.860834655667833	28.392139632433285	29.949211213442695	20.79781449845619
44-45	20.872780018631016	28.561743646660716	29.792071264629982	20.773405070078287
46-47	20.91442819578803	28.509279593757874	29.567928753701217	21.008363456752882
48-49	20.899804447735193	28.589965397923876	29.58511931867865	20.925110835662284
50-51	20.87992071971247	28.674524908954606	29.42424627307172	21.021308098261205
52-53	20.985327067036092	28.65953101884581	29.388800857775266	20.966341056342827
54-55	21.021039939819932	28.62045307291366	29.33254486285881	21.025962124407595
56-57	21.07629944888016	28.66800721489171	29.24156404496096	21.01412929126717
58-59	21.0101912708729	28.66306125899918	29.33631489891057	20.990432571217347
60-61	21.067758641685167	28.690211400873732	29.26620113735614	20.97582882008496
62-63	20.997391802213848	28.689045399760964	29.298697362894238	21.014865435130947
64-65	20.981718614366347	28.66590730031961	29.296453249350744	21.055920835963303
66-67	21.104698606014615	28.68464210252028	29.14352915120707	21.067130140258037
68-69	21.077702040834996	28.638600349666042	29.201324516557737	21.08237309294122
70-71	21.07548305360381	28.428697433819234	29.25065192912333	21.24516758345363
72-73	21.101111545048568	28.4546251873137	29.282570727846146	21.16169253979159
74-75	21.048257309408395	28.558242359322648	29.171083919201564	21.222416412067385
76-77	21.106343624999308	28.580701160393744	29.073229052489637	21.239726162117314
78-79	21.14376282332162	28.579475720701446	29.02766659259689	21.249094863380044
80-81	21.13310787775925	28.585584565780042	29.00372101602472	21.277586540435987
82-83	21.182981547106884	28.561780231387935	29.060292708893925	21.194945512611255
84-85	21.33987314555329	28.50805685588238	28.934551490853842	21.21751850771049
86-87	21.135836339528044	28.60329989402743	28.967253903277662	21.293609863166868
88-89	21.27268937801928	28.541409014923698	28.909297330988075	21.27660427606895
90-91	21.278404715521997	28.57892980163656	28.946961284988472	21.195704197852972
92-93	21.28300457406251	28.579460912042915	28.880287302560742	21.25724721133384
94-95	21.235107614342475	28.6128924028266	28.870075336478134	21.28192464635279
96-97	21.278950372971533	28.73910851646678	28.967249758441895	21.01469135211979
98-99	22.266117265439604	30.647066853731925	29.24072341140817	17.846092469420302
100	21.14920409676849	31.546101149396303	23.279886634205482	24.02480811962972
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2492.0
1	3382.0
2	5688.0
3	7431.0
4	8825.0
5	10749.5
6	12241.5
7	16245.5
8	19785.0
9	20280.0
10	20658.0
11	21528.0
12	24063.0
13	29412.0
14	40547.5
15	53240.5
16	59888.5
17	60153.5
18	56661.5
19	51965.5
20	49259.5
21	50468.0
22	57365.5
23	71672.0
24	93748.0
25	124560.0
26	164774.0
27	210739.5
28	259146.0
29	312530.0
30	365035.5
31	414463.0
32	465188.5
33	512010.0
34	553672.0
35	588080.5
36	617072.0
37	637864.5
38	643315.0
39	641273.0
40	640202.5
41	633694.0
42	620162.5
43	610303.0
44	602647.0
45	593214.0
46	577297.5
47	559647.5
48	541977.5
49	517629.0
50	492125.0
51	462423.5
52	429395.0
53	396791.0
54	361839.0
55	326247.5
56	292415.0
57	258954.5
58	224623.5
59	190778.5
60	158409.0
61	127209.0
62	98127.0
63	74293.0
64	55015.5
65	40139.5
66	29189.0
67	21429.5
68	15699.0
69	11206.5
70	8128.5
71	5940.0
72	4338.5
73	3182.5
74	2327.0
75	1718.0
76	1248.0
77	855.5
78	588.5
79	397.0
80	272.0
81	161.5
82	91.5
83	61.5
84	41.0
85	24.0
86	18.5
87	16.5
88	10.0
89	5.0
90	4.0
91	2.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0018913782163441867
3	0.0
4	0.0
5	0.003358710782760673
6	1.2115589997934291E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	7.958394895554717E-5
44-45	3.835119162382083E-5
46-47	0.0
48-49	0.0
50-51	3.5787929604569864E-6
52-53	0.0010531642735372028
54-55	0.00227200551853782
56-57	0.002640007770422871
58-59	0.0017538313444737884
60-61	0.004212059102806766
62-63	0.0017631556749293995
64-65	0.0017673217868816682
66-67	1.9568869412790412E-4
68-69	1.2404438339048808E-4
70-71	1.9196818436843818E-4
72-73	0.0
74-75	0.0
76-77	0.0
78-79	5.307219852072269E-5
80-81	2.3531343811882016E-4
82-83	4.174446966135091E-6
84-85	0.0
86-87	0.0
88-89	0.0
90-91	3.4903925635790274E-5
92-93	2.0754217996741942E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	118.0
20-21	437.0
22-23	837.0
24-25	1139.0
26-27	3237.0
28-29	8608.0
30-31	26920.0
32-33	35917.0
34-35	49227.0
36-37	76448.0
38-39	91235.0
40-41	88927.0
42-43	102371.0
44-45	124387.0
46-47	127341.0
48-49	120445.0
50-51	109990.0
52-53	106692.0
54-55	115784.0
56-57	122749.0
58-59	123151.0
60-61	120649.0
62-63	115399.0
64-65	121408.0
66-67	131415.0
68-69	134819.0
70-71	137040.0
72-73	124889.0
74-75	123326.0
76-77	130802.0
78-79	135481.0
80-81	135717.0
82-83	127768.0
84-85	123982.0
86-87	128056.0
88-89	135506.0
90-91	137781.0
92-93	135915.0
94-95	157195.0
96-97	435933.0
98-99	950857.0
100-101	9676993.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.39854173909787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.62135885655606	76.5353335502627
2	7.371920368897735	12.591024986412377
3	1.6899134057784766	4.329484215565029
4	0.6498878800878432	2.2199790901366203
5	0.2897127461916696	1.2370523023998978
6	0.13493898051512604	0.6914155295851383
7	0.07328029447919608	0.4380621200714507
8	0.046003262477463454	0.31428892246530704
9	0.03164488297260964	0.24321841734287442
>10	0.0887736548706597	1.1690241730189792
>50	0.001892215867537674	0.11037729202919
>100	6.356210823401034E-4	0.08825030492445841
>500	2.5221124250122663E-5	0.017952313684140114
>1k	1.2609099081645609E-5	0.0145367821019205
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.730883332185717E-6	0.0	0.0	0.0
3	0.0	6.730883332185717E-6	0.0	0.0	0.0
4	0.0	1.3461766664371435E-5	0.0	2.692353332874287E-5	0.0
5	0.0	1.3461766664371435E-5	0.0	4.711618332530002E-5	0.0
6	2.692353332874287E-5	1.3461766664371435E-5	0.0	4.711618332530002E-5	6.730883332185717E-6
7	2.692353332874287E-5	1.3461766664371435E-5	0.0	6.057794998967146E-5	6.730883332185717E-6
8	2.692353332874287E-5	1.3461766664371435E-5	0.0	6.057794998967146E-5	6.730883332185717E-6
9	2.692353332874287E-5	2.692353332874287E-5	0.0	1.4134854997590007E-4	6.730883332185717E-6
10-11	3.028897499483573E-5	2.692353332874287E-5	0.0	1.716375249707358E-4	6.730883332185717E-6
12-13	3.365441666092859E-5	3.028897499483573E-5	0.0	1.951956166333858E-4	6.730883332185717E-6
14-15	3.365441666092859E-5	4.711618332530002E-5	0.0	2.38946358292593E-4	6.730883332185717E-6
16-17	4.711618332530002E-5	4.711618332530002E-5	0.0	3.028897499483573E-4	1.3461766664371435E-5
18-19	5.048162499139288E-5	5.7212508323578604E-5	0.0	3.2981328327710016E-4	2.0192649996557154E-5
20-21	5.384706665748574E-5	6.730883332185718E-5	0.0	3.634676999380288E-4	2.0192649996557154E-5
22-23	5.384706665748574E-5	6.730883332185718E-5	0.0	4.2404564992770026E-4	2.355809166265001E-5
24-25	5.384706665748574E-5	8.077059998622862E-5	0.0	5.115471332461146E-4	2.692353332874287E-5
26-27	5.384706665748574E-5	8.750148331841433E-5	0.0	5.754905249018788E-4	2.692353332874287E-5
28-29	5.384706665748574E-5	8.750148331841433E-5	0.0	7.572243748708931E-4	2.692353332874287E-5
30-31	6.730883332185718E-5	1.1442501664715721E-4	0.0	0.0014202163830911866	2.692353332874287E-5
32-33	7.40397166540429E-5	1.1442501664715721E-4	0.0	0.002833701882850187	3.365441666092859E-5
34-35	7.40397166540429E-5	1.312522249776215E-4	0.0	0.004795754374182324	3.365441666092859E-5
36-37	7.740515832013576E-5	1.5817575830636435E-4	0.0	0.007397240782072104	3.701985832702145E-5
38-39	8.077059998622862E-5	1.783684083029215E-4	0.0	0.011799238481321563	4.711618332530002E-5
40-41	8.077059998622862E-5	1.9519561663338582E-4	0.0	0.016746437730478066	4.711618332530002E-5
42-43	9.423236665060005E-5	1.9856105829947867E-4	0.0	0.02154219210466039	4.711618332530002E-5
44-45	1.0096324998278576E-4	2.3221547496040724E-4	0.0	0.026691317853782464	4.711618332530002E-5
46-47	1.0096324998278576E-4	2.62504449955243E-4	0.0	0.032493339286126556	4.711618332530002E-5
48-49	1.0096324998278576E-4	2.62504449955243E-4	0.0	0.03832564969346548	4.711618332530002E-5
50-51	1.0769413331497148E-4	2.8269709995180014E-4	0.0	0.04447767705908322	5.048162499139288E-5
52-53	1.0769413331497148E-4	2.8269709995180014E-4	0.0	0.05051527940805381	5.7212508323578604E-5
54-55	1.0769413331497148E-4	2.8269709995180014E-4	0.0	0.056347589815392736	6.057794998967146E-5
56-57	1.0769413331497148E-4	2.8269709995180014E-4	0.0	0.062102495064411525	6.057794998967146E-5
58-59	1.2115589997934292E-4	2.8269709995180014E-4	0.0	0.06805932681339588	6.057794998967146E-5
60-61	1.2788678331152864E-4	2.8942798328398584E-4	0.0	0.07353826584579506	6.057794998967146E-5
62-63	1.2788678331152864E-4	2.8942798328398584E-4	0.0	0.0794547122947863	7.067427498795003E-5
64-65	1.2788678331152864E-4	3.1635151661272876E-4	0.0	0.08523317563546774	7.40397166540429E-5
66-67	1.2788678331152864E-4	3.197169582788216E-4	0.0	0.09075586540952613	7.40397166540429E-5
68-69	1.2788678331152864E-4	3.2981328327710016E-4	0.0	0.09638624931689949	7.40397166540429E-5
70-71	1.2788678331152864E-4	3.432750499414716E-4	0.0	0.10163633831600434	7.40397166540429E-5
72-73	1.3798310830980722E-4	3.500059332736573E-4	0.0	0.10574890803196982	8.077059998622862E-5
74-75	1.649066416385501E-4	3.5337137493975015E-4	0.0	0.10881145994811431	8.077059998622862E-5
76-77	1.817338499690144E-4	3.8366034993458594E-4	0.0	0.11191439716425193	8.077059998622862E-5
78-79	1.817338499690144E-4	3.9712211659895735E-4	0.0	0.11386298788891969	8.077059998622862E-5
80-81	1.817338499690144E-4	4.004875582650502E-4	0.0	0.11452597989713999	8.077059998622862E-5
82-83	1.817338499690144E-4	4.0385299993114305E-4	3.3654416660928587E-6	0.11509810498037577	8.077059998622862E-5
84-85	2.0192649996557152E-4	4.0385299993114305E-4	6.730883332185717E-6	0.11538080208032758	8.077059998622862E-5
86-87	2.0192649996557152E-4	4.240456499277002E-4	6.730883332185717E-6	0.1155120543053052	8.077059998622862E-5
88	2.0192649996557152E-4	4.307765332598859E-4	6.730883332185717E-6	0.11552215063030348	8.077059998622862E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	17430	0.0	48.68491	1
GTATCAA	32815	0.0	32.32107	1
ATCAACG	43280	0.0	28.883244	3
TCAACGC	43775	0.0	28.57609	4
CAACGCA	44390	0.0	28.22814	5
AACGCAG	45715	0.0	27.44677	6
TATCAAC	46635	0.0	27.107077	2
ACGCAGA	52405	0.0	23.96714	7
CGCAGAG	52900	0.0	23.718727	8
TACATGG	52025	0.0	21.00047	2
TGGTATC	8135	0.0	20.778654	2
ACATGGG	51740	0.0	20.498613	3
GCAGAGT	62470	0.0	20.08517	9
GAGTACT	35020	0.0	19.288162	12-13
AGAGTAC	58585	0.0	18.677279	10-11
GTACATG	51115	0.0	18.625498	1
CAGAGTA	62670	0.0	18.1562	10-11
CATGGGG	30365	0.0	18.074104	4
GTGGTAT	8625	0.0	17.919752	1
TGTATCA	11160	0.0	17.130365	1
>>END_MODULE