FastQCFastQC Report
Fri 27 May 2016
SRR2140083_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140083_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15879618
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA216200.049.11531
GTATCAA388100.035.948811
ATCAACG470800.028.9043543
TCAACGC478850.028.4096974
CAACGCA482900.028.1366565
TATCAAC497200.027.7070772
AACGCAG501650.027.2519366
ACGCAGA581600.023.426547
CGCAGAG588250.023.16128
TACATGG626550.022.0337872
GTACATG645100.021.8479291
TGGTATC97800.021.1073652
ACATGGG640250.021.051733
GTGGTAT106700.020.994841
GCAGAGT690350.019.6143269
CATGGGG403250.019.2405474
GAGTACT362450.018.33880612-13
CAGAGTA683150.017.53193910-11
AGAGTAC640700.017.5172210-11
GTACTTT411150.016.19771814-15
Produced by FastQC (version 0.11.5)