##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140083_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15879618
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08483453443276	33.0	31.0	34.0	30.0	34.0
2	32.29684612060567	33.0	31.0	34.0	30.0	34.0
3	32.38280517831097	34.0	31.0	34.0	30.0	34.0
4	35.97435379113024	37.0	35.0	37.0	35.0	37.0
5	35.70614897663155	37.0	35.0	37.0	33.0	37.0
6	35.7598578882691	37.0	35.0	37.0	33.0	37.0
7	35.74645718807594	37.0	35.0	37.0	33.0	37.0
8	35.73422748582491	37.0	35.0	37.0	33.0	37.0
9	37.55441900428587	39.0	37.0	39.0	35.0	39.0
10-11	37.36768242787704	39.0	37.0	39.0	34.5	39.0
12-13	37.374945795295574	39.0	37.0	39.0	34.5	39.0
14-15	38.5956964455946	40.0	38.0	41.0	34.5	41.0
16-17	38.53899719124226	40.0	38.0	41.0	34.0	41.0
18-19	38.5862207138736	40.0	38.0	41.0	34.0	41.0
20-21	38.603602103136495	40.0	38.0	41.0	34.0	41.0
22-23	38.53724749293818	40.0	38.0	41.0	34.0	41.0
24-25	38.54866228745473	40.0	38.0	41.0	34.0	41.0
26-27	38.422073633084324	40.0	38.0	41.0	34.0	41.0
28-29	38.327440790792856	40.0	38.0	41.0	34.0	41.0
30-31	38.20605821879025	40.0	38.0	41.0	34.0	41.0
32-33	38.113065666058205	40.0	38.0	41.0	34.0	41.0
34-35	38.05506378223221	40.0	37.5	41.0	34.0	41.0
36-37	38.0302854891234	40.0	37.0	41.0	33.5	41.0
38-39	37.94500826125455	40.0	37.0	41.0	33.0	41.0
40-41	37.9162468714094	40.0	37.0	41.0	33.0	41.0
42-43	37.86439372488135	40.0	37.0	41.0	33.0	41.0
44-45	37.76886769257608	40.0	37.0	41.0	33.0	41.0
46-47	37.69092993881105	40.0	37.0	41.0	33.0	41.0
48-49	37.70232827959443	40.0	37.0	41.0	33.0	41.0
50-51	37.63554195768796	39.0	37.0	41.0	33.0	41.0
52-53	37.44845073096195	39.0	36.0	40.0	32.0	41.0
54-55	37.28141677444163	39.0	36.0	40.0	31.5	41.0
56-57	37.155118077768144	39.0	36.0	40.0	31.0	41.0
58-59	36.99639803190932	39.0	35.5	40.0	31.0	41.0
60-61	36.81543448576692	38.5	35.0	40.0	31.0	41.0
62-63	36.67248972541343	38.0	35.0	40.0	31.0	41.0
64-65	36.47756527530976	38.0	35.0	40.0	31.0	41.0
66-67	36.20626380415198	37.5	34.5	40.0	30.5	41.0
68-69	35.974725365139705	37.0	34.0	40.0	30.0	41.0
70-71	35.702203479310484	36.5	34.0	39.0	30.0	41.0
72-73	35.40758221152147	36.0	34.0	39.0	30.0	40.0
74-75	34.9818965613122	36.0	34.0	38.5	29.5	40.0
76-77	33.01997674922671	34.0	31.0	36.5	27.0	39.0
78-79	34.169115438141645	35.0	33.0	37.0	29.0	39.0
80-81	34.18143543463858	35.0	33.0	37.0	29.0	39.0
82-83	33.93619469070535	35.0	33.0	36.0	29.0	38.5
84-85	33.664530285464	35.0	33.0	36.0	29.0	37.0
86-87	33.427213936262305	35.0	33.0	35.5	29.0	37.0
88-89	33.279516121766264	35.0	33.0	35.0	29.0	36.5
90-91	33.19132439434813	35.0	33.0	35.0	29.0	36.0
92-93	33.07036722713536	34.5	33.0	35.0	29.0	36.0
94-95	32.980538745020475	34.0	33.0	35.0	29.0	36.0
96-97	32.99439456575951	34.0	32.5	35.0	29.0	35.0
98-99	33.16776658569704	34.0	33.0	35.0	29.0	35.0
100-101	31.955659639879144	33.0	31.0	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	12.0
19	32.0
20	135.0
21	524.0
22	2214.0
23	6902.0
24	17332.0
25	36028.0
26	65230.0
27	104060.0
28	153567.0
29	213684.0
30	285036.0
31	373561.0
32	494007.0
33	674838.0
34	979075.0
35	1479291.0
36	2455808.0
37	3768189.0
38	3582001.0
39	1174925.0
40	13165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.635774865617044	20.957821529459967	14.198389407100349	23.208014197822642
2	14.665439818791604	21.716977001829314	41.99357699669051	21.624006182688575
3	18.684104200000807	26.799111515039154	30.270883124629716	24.245901160330323
4	12.330756584136509	18.394693206093944	39.425660734242854	29.848889475526697
5	12.78259968218379	38.56631815702368	35.66246996621707	12.988612194575461
6	29.124756023728025	38.62678560655552	19.210972203487515	13.037486166228938
7	26.19429128200426	32.958912777723334	23.451801251592325	17.394994688680075
8	24.03143026615937	37.56201324338827	21.35800367657084	17.04855281388152
9	25.662642135084912	16.748627107513713	21.555218099787325	36.033512657614054
10-11	23.964563713622862	27.354444761308038	29.0181392180665	19.6628523070026
12-13	24.77067429540723	24.879398710219238	29.34391349201661	21.006013502356925
14-15	21.77399000076576	26.234922809216542	27.18367371100158	24.807413479016116
16-17	21.14493872187691	29.394238482336355	27.867931025318953	21.59289177046778
18-19	21.119531968590177	28.59232507986023	29.663040382961352	20.625102568588236
20-21	22.173763391643575	27.64621925134481	29.495848433443268	20.684168923568343
22-23	21.80031591771678	27.625721967845273	29.316771093938314	21.257191020499633
24-25	21.41656175990729	27.763199942544748	29.503353840091386	21.316884457456574
26-27	21.4106839217446	27.93125615269912	29.348106953920773	21.309952971635507
28-29	21.280684004048588	28.103251600123553	29.384961529533015	21.231102866294844
30-31	21.60199498141749	27.920644252107856	29.31790852132023	21.15945224515442
32-33	21.386410669621515	28.034875816056733	29.227294000214854	21.351419514106894
34-35	21.464180347804223	28.121672572513884	29.289570544471495	21.1245765352104
36-37	21.43400491263161	28.078700621253866	29.221528395405016	21.265766070709507
38-39	21.319450475696637	28.16123836839111	29.283142280394042	21.23616887551821
40-41	21.55890008237629	28.01857841616957	29.128202177612806	21.294319323841336
42-43	21.452731841463745	28.12653848745221	28.954450901603202	21.466278769480844
44-45	21.57574073846491	28.290262457399812	28.71777254047852	21.416224263656762
46-47	21.570494928896718	28.25873208938146	28.468952928400647	21.70182005332117
48-49	21.60713687564352	28.316859329356316	28.437537342247644	21.638466452752525
50-51	21.637462189557986	28.43787944956333	28.280129912905554	21.644528447973133
52-53	21.681831042032712	28.400756849408076	28.147166232195598	21.770245876363617
54-55	21.66923542488713	28.33158667949473	28.188724363177826	21.810453532440306
56-57	21.7461261319385	28.3510894339424	28.123443436253183	21.77934099786592
58-59	21.680093687736722	28.3909905414577	28.197338694493784	21.731577076311794
60-61	21.792665325242062	28.343752099812747	28.154518181951143	21.709064392994044
62-63	21.68368108549537	28.344124677963862	28.13134715436041	21.84084708218036
64-65	21.598416283849183	28.37536243969901	28.153399343666308	21.872821932785502
66-67	21.743968216631007	28.431316082121516	27.958805280425874	21.865910420821596
68-69	21.708870186616476	28.450797936739242	27.979671048476185	21.860660828168093
70-71	21.66411889834609	28.242110300517997	28.059317833327928	22.034452967807987
72-73	21.695702594700688	28.248453452513118	28.098330763791797	21.957513188994398
74-75	21.67877936207282	28.279007768907192	28.018703488391054	22.023509380628933
76-77	21.73911949645978	28.262331027694398	27.895981301210576	22.102568174635245
78-79	21.8021103250858	28.23701975382248	27.882460392987173	22.078409528104544
80-81	21.736637961791335	28.257897338554045	27.908305891446687	22.097158808207933
82-83	21.84987244836351	28.179120467180947	28.020938591097394	21.950068493358156
84-85	21.957331679299863	28.174177425961865	27.901648633629815	21.96684226110846
86-87	21.764121891383308	28.24503253154952	27.95496924421076	22.035876332856414
88-89	21.89115793304905	28.122708658887817	27.98952358901233	21.9966098190508
90-91	21.859473476096543	28.04524733213268	28.091778171780202	22.003501019990583
92-93	21.845695169320585	27.973457218055504	28.136053261132925	22.04479435149098
94-95	21.696407382436377	28.08099423516272	28.190183392831013	22.03241498956989
96-97	21.65890684902631	28.13433403158298	28.238872875980274	21.967886243410437
98-99	21.877956615505585	28.702014918815394	28.307970303121877	21.112058162557144
100-101	25.76670858050541	35.802505264403315	28.932556479841708	9.498229675249563
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2540.0
1	2480.0
2	4019.0
3	6111.5
4	8916.5
5	14999.5
6	19817.5
7	23201.5
8	25556.5
9	25012.0
10	22425.0
11	19987.0
12	22027.0
13	30086.5
14	48124.0
15	71727.0
16	89834.5
17	99242.5
18	99885.5
19	93790.0
20	85670.5
21	78750.0
22	76516.5
23	84043.0
24	101878.0
25	127592.5
26	160824.5
27	197633.0
28	235898.0
29	276684.5
30	313786.5
31	348315.5
32	385262.5
33	422640.0
34	459482.0
35	494552.0
36	528178.5
37	557884.5
38	579804.5
39	596620.5
40	616283.5
41	635564.5
42	649316.0
43	661253.5
44	672469.5
45	681109.0
46	684730.0
47	684059.0
48	677425.5
49	663368.0
50	644800.0
51	621202.5
52	586957.0
53	547124.5
54	504222.0
55	457959.0
56	410610.0
57	360094.5
58	312111.0
59	268617.5
60	223645.0
61	178399.5
62	139730.5
63	108289.0
64	80769.5
65	58192.0
66	42068.5
67	31101.5
68	22935.0
69	16055.5
70	11008.5
71	7689.0
72	5286.0
73	3586.0
74	2488.5
75	1772.5
76	1243.5
77	845.5
78	598.0
79	412.0
80	282.5
81	188.5
82	134.5
83	93.0
84	66.0
85	50.0
86	39.5
87	31.5
88	21.0
89	13.5
90	10.5
91	8.0
92	6.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0027834422717221536
3	1.2594761410507483E-5
4	3.085716545574333E-4
5	0.0
6	0.0
7	0.0020529461099127195
8	9.257149636722998E-4
9	3.778428423152245E-5
10-11	1.2279892375244796E-4
12-13	1.2594761410507482E-4
14-15	1.2594761410507483E-5
16-17	3.274637966731945E-4
18-19	0.0
20-21	5.667900873074004E-5
22-23	0.0
24-25	6.299917593927913E-6
26-27	6.050248829087781E-4
28-29	1.828628215134326E-4
30-31	3.157094729037767E-5
32-33	0.0016743565208922504
34-35	5.017264311230432E-4
36-37	0.001256950232937762
38-39	0.003507282106887313
40-41	1.0031671929896606E-4
42-43	8.026294663352569E-4
44-45	0.0016440651094591532
46-47	8.997602438870122E-5
48-49	2.089308080473138E-4
50-51	2.2475759382693052E-4
52-53	0.0
54-55	1.5262674089876337E-4
56-57	0.0014955747798597984
58-59	0.0028376980041818893
60-61	0.0010835318676019896
62-63	9.180552553603223E-4
64-65	7.305502413098766E-6
66-67	6.649977931309348E-5
68-69	2.2437635993109402E-5
70-71	1.1361565890397775E-5
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	0.0
82-83	2.046654638613799E-5
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	435.0
20-21	1629.0
22-23	2886.0
24-25	5843.0
26-27	7493.0
28-29	14974.0
30-31	38491.0
32-33	47704.0
34-35	64861.0
36-37	97695.0
38-39	116776.0
40-41	123207.0
42-43	140893.0
44-45	169084.0
46-47	169868.0
48-49	158749.0
50-51	143017.0
52-53	127665.0
54-55	137468.0
56-57	143276.0
58-59	146831.0
60-61	151925.0
62-63	143525.0
64-65	151775.0
66-67	161690.0
68-69	167432.0
70-71	167043.0
72-73	159227.0
74-75	160794.0
76-77	156139.0
78-79	169003.0
80-81	174956.0
82-83	166688.0
84-85	165562.0
86-87	174618.0
88-89	189209.0
90-91	208550.0
92-93	216050.0
94-95	240392.0
96-97	348274.0
98-99	1342794.0
100-101	9205127.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.28427112019344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.35226684960271	75.30990684355787
2	7.232347724480717	12.191463128912938
3	1.860310693178928	4.703847924950633
4	0.7278654649787807	2.4539044075718888
5	0.33781619709144756	1.4236295972224133
6	0.17174962624553178	0.8685475237962176
7	0.10171535321598708	0.6001103085279748
8	0.05657802462255308	0.38149100533857955
9	0.039208384803356	0.2974185121455837
>10	0.11688620917294046	1.4564059675348429
>50	0.0024900936692009083	0.14559820422666517
>100	6.927911582421096E-4	0.10389638980500043
>500	4.840273127764694E-5	0.03054376453964781
>1k	2.4185048343144053E-5	0.033236421869746
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.297380705253741E-6	0.0
2	0.0	6.297380705253741E-6	0.0	6.297380705253741E-6	0.0
3	0.0	6.297380705253741E-6	0.0	6.297380705253741E-6	0.0
4	0.0	1.8892142115761225E-5	0.0	1.2594761410507483E-5	0.0
5	0.0	1.8892142115761225E-5	0.0	1.8892142115761225E-5	0.0
6	0.0	1.8892142115761225E-5	0.0	1.8892142115761225E-5	0.0
7	0.0	1.8892142115761225E-5	0.0	4.4081664936776186E-5	0.0
8	0.0	1.8892142115761225E-5	0.0	5.667642634728367E-5	0.0
9	0.0	4.4081664936776186E-5	0.0	1.0075809128405986E-4	0.0
10-11	0.0	4.4081664936776186E-5	0.0	1.3224499481032857E-4	0.0
12-13	0.0	4.4081664936776186E-5	0.0	1.511371369260898E-4	0.0
14-15	0.0	5.35277359946568E-5	0.0	1.8577273080498536E-4	0.0
16-17	0.0	5.667642634728367E-5	0.0	1.9836749221549283E-4	0.0
18-19	0.0	6.612249740516429E-5	0.0	2.3615177644701528E-4	0.0
20-21	0.0	7.55685684630449E-5	0.0	3.4635593878895577E-4	0.0
22-23	0.0	8.186594916829864E-5	0.0	6.958605679305384E-4	0.0
24-25	0.0	8.816332987355237E-5	0.0	0.0015617504149029278	0.0
26-27	0.0	8.816332987355237E-5	0.0	0.0037847258038574984	0.0
28-29	0.0	8.816332987355237E-5	0.0	0.020762464185221582	0.0
30-31	0.0	9.760940093143298E-5	0.0	0.03059897284682793	0.0
32-33	0.0	1.0075809128405986E-4	0.0	0.03549833503551533	0.0
34-35	0.0	1.1965023339982107E-4	0.0	0.041852392167116365	0.0
36-37	0.0	1.385423755155823E-4	0.0	0.051433856910159934	0.0
38-39	0.0	1.511371369260898E-4	0.0	0.06987573630549551	0.0
40-41	0.0	1.826240404523585E-4	0.0	0.11061034339742934	0.0
42-43	0.0	1.920701115102391E-4	0.0	0.12722598238824134	0.0
44-45	0.0	2.2985439574176156E-4	0.0	0.14987136340433377	0.0
46-47	0.0	2.550439185627765E-4	0.0	0.1753348222860273	0.0
48-49	0.0	2.5819260891540337E-4	0.0	0.19802743365740916	0.0
50-51	0.0	2.896795124416721E-4	0.0	0.22112622608427984	0.0
52-53	0.0	2.959768931469258E-4	0.0	0.2517409423828709	0.0
54-55	0.0	3.0542296420480646E-4	0.0	0.3132317162793211	0.0
56-57	0.0	3.085716545574333E-4	0.0	0.3776759617265352	0.0
58-59	0.0	3.085716545574333E-4	0.0	0.44721478816429966	0.0
60-61	0.0	3.117203449100602E-4	0.0	0.49239534603414264	0.0
62-63	0.0	3.2116641596794076E-4	0.0	0.5129027663008014	0.0
64-65	0.0	3.526533194942095E-4	0.0	0.5317634215130365	0.0
66-67	0.0	3.526533194942095E-4	0.0	0.540450658195934	0.0
68-69	0.0	3.589507001994632E-4	0.0	0.5482971945546801	0.0
70-71	0.0	3.7469415196259763E-4	0.0	0.5553723017770327	0.0
72-73	0.0	3.841402230204782E-4	0.0	0.560728224066851	0.0
74-75	0.0	3.841402230204782E-4	0.0	0.5647648450989187	0.0
76-77	0.0	3.9043760372573194E-4	0.0	0.5684330693597289	0.0
78-79	0.0	3.9673498443098566E-4	0.0	0.5717612350624555	0.0
80-81	0.0	3.9673498443098566E-4	0.0	0.5734237435686426	0.0
82-83	0.0	3.9673498443098566E-4	0.0	0.5741164554462204	0.0
84-85	0.0	3.9673498443098566E-4	0.0	0.5746958144711038	0.0
86-87	0.0	4.061810554888663E-4	0.0	0.5749508583896665	0.0
88-89	0.0	4.156271265467469E-4	0.0	0.5750453191002454	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	21620	0.0	49.1153	1
GTATCAA	38810	0.0	35.94881	1
ATCAACG	47080	0.0	28.904354	3
TCAACGC	47885	0.0	28.409697	4
CAACGCA	48290	0.0	28.136656	5
TATCAAC	49720	0.0	27.707077	2
AACGCAG	50165	0.0	27.251936	6
ACGCAGA	58160	0.0	23.42654	7
CGCAGAG	58825	0.0	23.1612	8
TACATGG	62655	0.0	22.033787	2
GTACATG	64510	0.0	21.847929	1
TGGTATC	9780	0.0	21.107365	2
ACATGGG	64025	0.0	21.05173	3
GTGGTAT	10670	0.0	20.99484	1
GCAGAGT	69035	0.0	19.614326	9
CATGGGG	40325	0.0	19.240547	4
GAGTACT	36245	0.0	18.338806	12-13
CAGAGTA	68315	0.0	17.531939	10-11
AGAGTAC	64070	0.0	17.51722	10-11
GTACTTT	41115	0.0	16.197718	14-15
>>END_MODULE