FastQCFastQC Report
Fri 27 May 2016
SRR2140082_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140082_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5819525
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79550.049.054331
GTATCAA141950.035.378531
ATCAACG163400.030.0198883
TCAACGC164900.029.7964674
CAACGCA167900.029.2645745
AACGCAG173150.028.4255166
TATCAAC178000.027.861542
ACGCAGA204700.023.9422957
TACATGG242850.023.690282
CGCAGAG207300.023.6016948
GTACATG250250.023.3234621
ACATGGG248300.022.6664733
CATGGGG158000.020.335154
GCAGAGT247400.019.6915869
GTGGTAT39900.019.2670651
TGGTATC37400.018.4326062
GAGTACT125500.018.1271112-13
CAGAGTA244600.017.74727210-11
AGAGTAC229850.017.08660910-11
ATGGGGG92800.016.9737955
Produced by FastQC (version 0.11.5)