##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2140082_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5819525 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.732445173789955 34.0 31.0 34.0 31.0 34.0 2 32.90790863515493 34.0 31.0 34.0 31.0 34.0 3 33.0031217324438 34.0 33.0 34.0 31.0 34.0 4 36.39327986390642 37.0 37.0 37.0 35.0 37.0 5 36.24383364621683 37.0 37.0 37.0 35.0 37.0 6 36.30172874933951 37.0 37.0 37.0 35.0 37.0 7 36.30599679527109 37.0 37.0 37.0 35.0 37.0 8 36.29338786241145 37.0 37.0 37.0 35.0 37.0 9 38.1911217839944 39.0 39.0 39.0 37.0 39.0 10-11 38.092060778156295 39.0 38.0 39.0 37.0 39.0 12-13 38.1293706101443 39.0 38.0 39.0 37.0 39.0 14-15 39.55180379841997 41.0 39.0 41.0 37.0 41.0 16-17 39.537809958716565 41.0 39.0 41.0 37.0 41.0 18-19 39.60039668185978 41.0 39.5 41.0 37.0 41.0 20-21 39.6238354794138 41.0 40.0 41.0 37.0 41.0 22-23 39.560648306394626 41.0 39.0 41.0 37.0 41.0 24-25 39.51843043648027 41.0 39.0 41.0 37.0 41.0 26-27 39.48269182861296 41.0 39.0 41.0 37.0 41.0 28-29 39.39025585652674 40.5 39.0 41.0 36.5 41.0 30-31 39.29358404647185 40.0 39.0 41.0 36.0 41.0 32-33 39.18082445796142 40.0 39.0 41.0 35.5 41.0 34-35 39.10944108057327 40.0 39.0 41.0 35.0 41.0 36-37 39.11796027299941 40.0 39.0 41.0 35.0 41.0 38-39 39.11814502268351 40.0 39.0 41.0 35.0 41.0 40-41 39.07669575556443 40.0 39.0 41.0 35.0 41.0 42-43 39.046615134305114 40.0 39.0 41.0 35.0 41.0 44-45 39.01502859128399 40.0 39.0 41.0 35.0 41.0 46-47 38.951666810999356 40.0 38.0 41.0 35.0 41.0 48-49 38.931681350029336 40.0 38.5 41.0 35.0 41.0 50-51 38.851202551205525 40.0 38.0 41.0 35.0 41.0 52-53 38.69716440521539 40.0 38.0 41.0 35.0 41.0 54-55 38.579763242040045 40.0 38.0 41.0 34.5 41.0 56-57 38.4336068175234 40.0 38.0 41.0 34.0 41.0 58-59 38.26281804297673 40.0 37.0 41.0 34.0 41.0 60-61 38.15182095380128 40.0 37.0 41.0 34.0 41.0 62-63 38.002232323864405 39.5 36.5 41.0 34.0 41.0 64-65 37.76876156349455 39.0 36.0 41.0 34.0 41.0 66-67 37.51161132197498 39.0 35.5 40.5 33.5 41.0 68-69 37.270784904900694 38.5 35.0 40.0 33.0 41.0 70-71 36.98166435365884 37.5 35.0 40.0 33.0 41.0 72-73 36.6348085729789 37.0 35.0 39.5 33.0 41.0 74-75 36.256368787781966 36.5 35.0 39.0 32.5 41.0 76-77 34.31066477864027 34.5 33.0 37.0 30.0 39.0 78-79 35.37289346500097 36.0 34.0 37.5 31.5 39.0 80-81 35.28551726742399 35.0 35.0 37.0 32.0 39.0 82-83 34.9955443484478 35.0 34.0 37.0 32.0 39.0 84-85 34.73601858231292 35.0 34.0 36.0 32.0 38.0 86-87 34.511782775070685 35.0 34.0 36.0 32.0 37.0 88-89 34.3415837482161 35.0 34.0 36.0 31.5 37.0 90-91 34.18214497184893 35.0 34.0 35.0 31.0 36.5 92-93 34.083603610668305 35.0 34.0 35.0 31.0 36.0 94-95 33.99550035486753 35.0 34.0 35.0 31.0 36.0 96-97 33.950257657167256 35.0 34.0 35.0 31.0 36.0 98-99 33.966590880175346 35.0 34.0 35.0 31.0 36.0 100-101 32.73425919590583 34.5 32.0 35.0 28.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 8.0 20 22.0 21 66.0 22 244.0 23 838.0 24 2400.0 25 4955.0 26 9439.0 27 16023.0 28 24221.0 29 34750.0 30 47734.0 31 64105.0 32 85498.0 33 120449.0 34 186641.0 35 300927.0 36 589627.0 37 1326363.0 38 1989230.0 39 993947.0 40 22037.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.81052147726833 20.13949592105885 13.159630725875394 23.89035187579742 2 14.711657540199614 21.64418686692599 41.27033822886798 22.373817364006417 3 18.505624584784975 26.64595274255861 30.23920165986341 24.609221012793007 4 11.890856328062682 17.9442057268621 39.72819417127386 30.436743773801357 5 12.557949317169356 38.69968768928735 35.86124297086102 12.881120022682262 6 29.107684905555008 38.47714375314136 19.434318092971505 12.980853248332123 7 26.384507326628892 32.90761703059958 23.480335594399886 17.227540048371644 8 24.531423796342324 36.71922510061447 21.708822988127313 17.040528114915894 9 26.101642081864036 16.221484724921776 21.380315191598555 36.29655800161564 10-11 23.967838682369226 27.09572366100691 29.254827174717036 19.68161048190683 12-13 24.731818033805084 24.743597377064166 29.48462409074167 21.039960498389082 14-15 21.739845420161156 26.058210672810134 27.212928495612264 24.989015411416446 16-17 21.178343620692026 29.224624706157293 27.943161197641007 21.65387047550967 18-19 21.15206997134646 28.462048019383023 29.657875857565696 20.72800615170482 20-21 22.165020290084193 27.624457735363688 29.42137817915934 20.789143795392782 22-23 21.79381797990585 27.574929449809044 29.28669421937831 21.344558350906794 24-25 21.43799617988488 27.711261175125852 29.46893524496649 21.381807400022776 26-27 21.363244542437126 27.94001045893364 29.355878069431814 21.34086692919742 28-29 21.24989849595085 28.056605195355726 29.4034779072136 21.290018401479827 30-31 21.519916845089178 27.9071957150342 29.335158707446297 21.23772873243033 32-33 21.300171067640164 28.045592587823197 29.261375928977092 21.392860415559547 34-35 21.45691798125899 28.1176336627606 29.24293659193821 21.182511764042196 36-37 21.363758044663633 28.078055378531786 29.221807950795075 21.33637862600951 38-39 21.258408220515264 28.136389410597 29.31963130475614 21.2855710641316 40-41 21.46543273190972 28.045460685912566 29.210818282261116 21.2782882999166 42-43 21.4444431555456 28.099494860714152 28.994129462353698 21.46193252138655 44-45 21.479827548655194 28.314434506423652 28.77773703045015 21.428000914471 46-47 21.506991067296465 28.271531855067916 28.551754752554615 21.669722325081004 48-49 21.497136105380246 28.334750280389358 28.578769794565755 21.58934381966464 50-51 21.470606767647585 28.479708679090994 28.440511790571556 21.609172762689866 52-53 21.610874968080143 28.422478218115693 28.33453594656852 21.63211086723565 54-55 21.620995302373956 28.307540204135286 28.3591931360559 21.712271357434858 56-57 21.660581581783127 28.43612788894689 28.220822092608643 21.68246843666134 58-59 21.578480554990623 28.504477000195582 28.29752629278202 21.619516152031775 60-61 21.64403093686597 28.47935785014598 28.25018894585457 21.62642226713347 62-63 21.562971006433493 28.472238001332222 28.280187962087084 21.6846030301472 64-65 21.53282998374282 28.455094260917928 28.29440661289924 21.71766914244001 66-67 21.66944876868541 28.47879296186631 28.13888587619089 21.712872393257392 68-69 21.67968949581467 28.450648894747992 28.176584753293245 21.693076856144092 70-71 21.58713755958188 28.25677415024435 28.295478651414737 21.860609638759033 72-73 21.638769374443125 28.295991355783517 28.304313690611377 21.760925579161974 74-75 21.59413174219904 28.342347470280348 28.21760970247883 21.845911085041774 76-77 21.617282648925475 28.36637027144179 28.14898773936485 21.867359340267882 78-79 21.665249134485098 28.345637776328143 28.12944855544646 21.859664533740304 80-81 21.63020043787535 28.360234540129554 28.161262039512113 21.84830298248298 82-83 21.740863974894097 28.2636909851865 28.239307555758753 21.756137484160647 84-85 21.84112150601452 28.2339070542399 28.16338409568343 21.761587344062157 86-87 21.646069225012567 28.294826080207226 28.244224491261726 21.814880203518477 88-89 21.751127636889407 28.18501327225917 28.19757128699798 21.866287803853442 90-91 21.7756420587249 28.19324108779796 28.245716854884762 21.785399998592375 92-93 21.753368582223302 28.127835844971322 28.295048024399982 21.823747548405393 94-95 21.658360746954912 28.16079498290989 28.350122392543508 21.830721877591685 96-97 21.674433995184817 28.217341032341807 28.381685444644333 21.726539527829043 98-99 21.915585537165466 28.732310019487116 28.44249957079844 20.90960487254897 100-101 25.75981869922323 35.70089661706612 29.179099200307263 9.360185483403384 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 942.0 1 900.0 2 1450.5 3 2227.0 4 3160.0 5 5194.5 6 6814.0 7 8021.0 8 8924.0 9 8767.5 10 7819.0 11 6951.0 12 7606.5 13 10261.0 14 16360.0 15 24115.0 16 29797.0 17 32871.0 18 33344.0 19 31653.5 20 29143.5 21 27005.5 22 26699.5 23 29705.0 24 36068.0 25 45242.0 26 56671.0 27 69826.5 28 84567.0 29 99945.5 30 114587.0 31 128869.0 32 143449.5 33 157930.5 34 171882.5 35 185282.0 36 198574.0 37 209868.5 38 218075.5 39 224860.0 40 231912.0 41 238241.0 42 242169.5 43 246216.5 44 249534.0 45 250912.0 46 250943.0 47 249779.5 48 247485.5 49 242171.0 50 235766.0 51 226141.5 52 212107.5 53 197874.0 54 182564.5 55 165980.0 56 149973.5 57 133180.5 58 116118.5 59 100832.5 60 85881.0 61 70542.5 62 55721.5 63 43013.5 64 32745.0 65 24519.5 66 18349.0 67 13895.5 68 10412.5 69 7300.5 70 5071.5 71 3613.5 72 2541.0 73 1836.0 74 1315.5 75 919.5 76 636.0 77 410.5 78 287.0 79 206.0 80 142.5 81 105.5 82 83.5 83 56.0 84 35.0 85 27.0 86 21.5 87 16.5 88 12.0 89 9.5 90 8.0 91 7.5 92 6.5 93 4.0 94 1.5 95 1.0 96 1.0 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 6.873413208122655E-5 3 0.004313066788096967 4 5.155059906091992E-5 5 0.0 6 0.0 7 0.0 8 5.842401226904258E-4 9 0.002474428754924156 10-11 8.505848845051787E-4 12-13 2.6634476181475295E-4 14-15 1.374682641624531E-4 16-17 0.0014606003067260643 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 6.633190019099279E-4 32-33 7.341163562333301E-4 34-35 2.5996919018470723E-5 36-37 6.618390731326951E-4 38-39 6.053697879415953E-4 40-41 8.846762571470782E-6 42-43 8.030824804076876E-5 44-45 9.10496154596117E-4 46-47 0.0 48-49 4.4308245819932394E-4 50-51 2.8004437396453595E-5 52-53 1.8859587167408824E-5 54-55 6.095827939160589E-4 56-57 8.184634571472754E-4 58-59 7.792179457009767E-5 60-61 1.280987203627598E-4 62-63 2.989725211366098E-5 64-65 0.0 66-67 0.0 68-69 4.128713894267972E-5 70-71 4.4956403788800253E-4 72-73 0.0 74-75 1.0715381362029981E-5 76-77 0.0 78-79 3.18586687932145E-4 80-81 0.0 82-83 0.0 84-85 1.1435535996723032E-5 86-87 2.4327627809536917E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 1.2321188748290436E-5 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 124.0 20-21 360.0 22-23 743.0 24-25 1669.0 26-27 2805.0 28-29 6416.0 30-31 14051.0 32-33 17984.0 34-35 24749.0 36-37 39685.0 38-39 47461.0 40-41 47367.0 42-43 54108.0 44-45 64534.0 46-47 65139.0 48-49 61846.0 50-51 55328.0 52-53 52000.0 54-55 55898.0 56-57 59278.0 58-59 58866.0 60-61 58152.0 62-63 54914.0 64-65 55906.0 66-67 60692.0 68-69 61446.0 70-71 61560.0 72-73 56334.0 74-75 56805.0 76-77 56933.0 78-79 60640.0 80-81 61797.0 82-83 57669.0 84-85 55962.0 86-87 58507.0 88-89 62940.0 90-91 68690.0 92-93 65014.0 94-95 68870.0 96-97 102532.0 98-99 463285.0 100-101 3440466.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.29230704897037 #Duplication Level Percentage of deduplicated Percentage of total 1 91.9366235963417 82.09233223210158 2 6.0910864209941264 10.87774317930443 3 1.246766392471098 3.3397994260459916 4 0.3788408652929458 1.3531029946574136 5 0.1565580535620995 0.6989714894828069 6 0.07001695475239471 0.3751185253430816 7 0.03700222716595073 0.23128099607184777 8 0.022183770032130602 0.15846720041702025 9 0.014245469747913632 0.11448097729087703 >10 0.04521190024416544 0.605275882937198 >50 0.0010936606315063479 0.06958241721203443 >100 3.448801650407227E-4 0.06711394131462212 >500 2.580859894760347E-5 0.016730737820982618 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.4367066040613284E-5 0.0 3 0.0 0.0 0.0 5.155059906091992E-5 0.0 4 0.0 0.0 0.0 5.155059906091992E-5 0.0 5 0.0 0.0 0.0 5.155059906091992E-5 0.0 6 0.0 0.0 0.0 5.155059906091992E-5 0.0 7 0.0 0.0 0.0 6.873413208122657E-5 0.0 8 0.0 0.0 0.0 6.873413208122657E-5 0.0 9 0.0 1.7183533020306642E-5 0.0 1.3746826416245313E-4 0.0 10-11 0.0 1.7183533020306642E-5 0.0 1.718353302030664E-4 0.0 12-13 0.0 1.7183533020306642E-5 0.0 1.9761062973352637E-4 0.0 14-15 0.0 6.873413208122657E-5 0.0 2.14794162753833E-4 0.0 16-17 0.0 6.873413208122657E-5 0.0 2.2338592926398633E-4 0.0 18-19 0.0 7.732589859137988E-5 0.0 2.3197769577413966E-4 0.0 20-21 0.0 8.59176651015332E-5 0.0 2.7493652832490627E-4 0.0 22-23 0.0 8.59176651015332E-5 0.0 3.2648712738582615E-4 0.0 24-25 0.0 9.450943161168652E-5 0.0 3.6085419342643944E-4 0.0 26-27 0.0 1.0310119812183984E-4 0.0 4.29588325507666E-4 0.0 28-29 0.0 1.0310119812183984E-4 0.0 8.935437170559453E-4 0.0 30-31 0.0 1.0310119812183984E-4 0.0 0.0022338592926398632 0.0 32-33 0.0 1.0310119812183984E-4 0.0 0.004407576219708653 0.0 34-35 0.0 1.4606003067260643E-4 0.0 0.007552162762424769 0.0 36-37 0.0 1.8901886322337304E-4 0.0 0.01201128958119434 0.0 38-39 0.0 2.062023962436797E-4 0.0 0.01884174395676623 0.0 40-41 0.0 2.2338592926398633E-4 0.0 0.026136153723886398 0.0 42-43 0.0 2.2338592926398633E-4 0.0 0.03266589627160292 0.0 44-45 0.0 2.5775299530459957E-4 0.0 0.04055313792792367 0.0 46-47 0.0 2.9212006134521286E-4 0.0 0.04906757853948561 0.0 48-49 0.0 2.9212006134521286E-4 0.0 0.05750469325245617 0.0 50-51 0.0 3.093035943655195E-4 0.0 0.06610505152911965 0.0 52-53 0.0 3.093035943655195E-4 0.0 0.07391496728684901 0.0 54-55 0.0 3.1789536087567283E-4 0.0 0.08115782645490827 0.0 56-57 0.0 3.2648712738582615E-4 0.0 0.08924267874096253 0.0 58-59 0.0 3.2648712738582615E-4 0.0 0.09674329090432637 0.0 60-61 0.0 3.522624269162861E-4 0.0 0.10475940905829943 0.0 62-63 0.0 3.780377264467461E-4 0.0 0.11244904008488665 0.0 64-65 0.0 4.124047924873594E-4 0.0 0.11976063338502713 0.0 66-67 0.0 4.29588325507666E-4 0.0 0.12689179958845437 0.0 68-69 0.0 4.29588325507666E-4 0.0 0.13384253869516843 0.0 70-71 0.0 4.3818009201781934E-4 0.0 0.1411541319953089 0.0 72-73 0.0 4.4677185852797267E-4 0.0 0.14627482483536028 0.0 74-75 0.0 4.4677185852797267E-4 0.0 0.15001224326727697 0.0 76-77 0.0 4.4677185852797267E-4 0.0 0.15374106993268352 0.0 78-79 0.0 4.4677185852797267E-4 0.0 0.1570918588716433 0.0 80-81 0.0 4.4677185852797267E-4 0.0 0.15881021217367397 0.0 82-83 0.0 4.4677185852797267E-4 0.0 0.159428819362405 0.0 84-85 0.0 4.4677185852797267E-4 0.0 0.15994432535301423 0.0 86-87 0.0 4.4677185852797267E-4 0.0 0.16021067011482898 0.0 88-89 0.0 4.983224575888926E-4 0.0 0.16030517954644063 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7955 0.0 49.05433 1 GTATCAA 14195 0.0 35.37853 1 ATCAACG 16340 0.0 30.019888 3 TCAACGC 16490 0.0 29.796467 4 CAACGCA 16790 0.0 29.264574 5 AACGCAG 17315 0.0 28.425516 6 TATCAAC 17800 0.0 27.86154 2 ACGCAGA 20470 0.0 23.942295 7 TACATGG 24285 0.0 23.69028 2 CGCAGAG 20730 0.0 23.601694 8 GTACATG 25025 0.0 23.323462 1 ACATGGG 24830 0.0 22.666473 3 CATGGGG 15800 0.0 20.33515 4 GCAGAGT 24740 0.0 19.691586 9 GTGGTAT 3990 0.0 19.267065 1 TGGTATC 3740 0.0 18.432606 2 GAGTACT 12550 0.0 18.12711 12-13 CAGAGTA 24460 0.0 17.747272 10-11 AGAGTAC 22985 0.0 17.086609 10-11 ATGGGGG 9280 0.0 16.973795 5 >>END_MODULE