FastQCFastQC Report
Fri 27 May 2016
SRR2140081_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140081_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71310482
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA1256400.050.716811
GTATCAA2271200.035.1980131
ATCAACG2964400.026.5921943
TCAACGC2997850.026.2939514
CAACGCA3012950.026.1390425
TATCAAC3036750.026.1039682
AACGCAG3117500.025.3632376
ACGCAGA3526600.022.2831177
CGCAGAG3559550.022.0545868
GCAGAGT3967000.019.3449959
AGAGTAC3696550.018.27538910-11
GAGTACT2433800.018.1500712-13
GTACATG2591550.017.7912351
TACATGG2539500.017.746562
CAGAGTA3891450.017.67989310-11
GTGGTAT492750.017.6366751
TGGTATC467050.017.3050352
ACATGGG2556250.017.2233
AGTACTT2591100.017.01987612-13
GTACTTT2726300.016.13895214-15
Produced by FastQC (version 0.11.5)