##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140081_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	71310482
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.101496214820145	31.0	31.0	34.0	28.0	34.0
2	31.167434641656186	31.0	31.0	34.0	28.0	34.0
3	31.177969362204003	31.0	31.0	34.0	27.0	34.0
4	34.855788620248	37.0	35.0	37.0	32.0	37.0
5	34.56930201369274	37.0	35.0	37.0	30.0	37.0
6	34.6336199214023	37.0	35.0	37.0	30.0	37.0
7	34.48144347138195	37.0	35.0	37.0	30.0	37.0
8	34.4667927500476	37.0	35.0	37.0	30.0	37.0
9	35.87816008591837	38.0	35.0	39.0	30.0	39.0
10-11	35.818244791838595	38.0	35.0	39.0	30.0	39.0
12-13	35.77499973986994	38.0	35.0	39.0	30.0	39.0
14-15	36.81317618214949	39.0	36.0	41.0	30.0	41.0
16-17	36.85777633644378	39.0	36.0	41.0	30.0	41.0
18-19	36.93620635603052	39.0	36.0	41.0	30.0	41.0
20-21	37.007538405056266	39.0	36.0	41.0	30.0	41.0
22-23	36.990663473849516	39.0	36.0	41.0	30.0	41.0
24-25	36.96934984957698	39.0	36.0	41.0	30.0	41.0
26-27	36.8765615262747	39.0	36.0	41.0	30.0	41.0
28-29	36.83399506958245	39.0	36.0	41.0	30.0	41.0
30-31	36.73203259235143	39.0	35.0	41.0	30.0	41.0
32-33	36.623338515639105	39.0	35.0	41.0	30.0	41.0
34-35	36.57209807401519	39.0	35.0	41.0	30.0	41.0
36-37	36.561783697219	39.0	35.0	41.0	30.0	41.0
38-39	36.51784577784453	39.0	35.0	41.0	30.0	41.0
40-41	36.505394515343454	39.0	35.0	41.0	30.0	41.0
42-43	36.477935410809486	39.0	35.0	41.0	30.0	41.0
44-45	36.45755242375027	39.0	35.0	41.0	29.5	41.0
46-47	36.432377764406425	39.0	35.0	40.5	29.0	41.0
48-49	36.38251099562596	39.0	35.0	40.0	29.0	41.0
50-51	36.33953812189258	39.0	35.0	40.0	29.0	41.0
52-53	36.28160691094534	38.5	34.5	40.0	29.0	41.0
54-55	36.203407371141026	38.0	34.0	40.0	28.5	41.0
56-57	36.12074896282412	38.0	34.0	40.0	28.0	41.0
58-59	35.99634041262284	38.0	34.0	40.0	28.0	41.0
60-61	35.847077363043965	38.0	34.0	40.0	28.0	41.0
62-63	35.697333613819836	38.0	34.0	40.0	28.0	41.0
64-65	35.526682170737175	37.0	34.0	40.0	28.0	41.0
66-67	35.32531177404081	37.0	33.0	40.0	28.0	41.0
68-69	35.098336131165496	36.5	33.0	39.5	28.0	41.0
70-71	34.88156186433341	36.0	33.0	39.0	28.0	41.0
72-73	34.60707863793604	36.0	33.0	39.0	28.0	40.0
74-75	34.33812832359036	35.0	32.0	38.5	28.0	40.0
76-77	34.05879882892586	35.0	32.0	37.5	27.5	39.5
78-79	33.78298897322894	35.0	32.0	37.0	27.0	39.0
80-81	33.527742040142144	35.0	32.0	36.5	27.0	39.0
82-83	33.403647120946786	35.0	32.0	36.0	27.5	38.5
84-85	33.362401429640045	35.0	32.0	36.0	28.0	37.0
86-87	33.194161317774295	35.0	32.0	36.0	28.0	37.0
88-89	33.04700965340436	35.0	32.0	35.0	28.0	37.0
90-91	32.96337102336052	35.0	32.0	35.0	28.0	36.0
92-93	32.91074924623522	34.0	32.0	35.0	28.0	36.0
94-95	32.88244586365967	34.0	32.0	35.0	28.5	36.0
96-97	32.93432185018954	34.0	32.0	35.0	29.0	35.5
98-99	33.04099701192138	34.0	32.0	35.0	29.0	35.0
100	32.69054477990473	34.0	32.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	9.0
13	34.0
14	86.0
15	176.0
16	364.0
17	1376.0
18	9668.0
19	49773.0
20	157558.0
21	348401.0
22	572356.0
23	773038.0
24	947623.0
25	1110069.0
26	1269609.0
27	1430335.0
28	1612958.0
29	1824688.0
30	2082550.0
31	2431375.0
32	2902597.0
33	3541117.0
34	4458677.0
35	5732919.0
36	7837884.0
37	1.13751E7
38	1.3461447E7
39	7122715.0
40	255978.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.52202286334286	21.80913319306971	14.874187780696813	23.79465616289061
2	14.10055116441367	22.918266069215463	40.816521195299174	22.164661571071697
3	16.513634871306813	28.417234567436463	32.10801437786296	22.961116183393763
4	11.736029425666212	19.951652969318403	40.103498275426126	28.208819329589264
5	11.503804801953388	39.62719313664472	36.63143743217138	12.237564629230512
6	27.085599704457252	40.9484141991386	19.48357856111919	12.48240753528496
7	23.79744658984801	34.532652229486594	24.93633304003458	16.733568140630815
8	23.60007768668909	37.309450346760784	22.031879674431124	17.058592292119005
9	25.26981201262529	16.258275013224104	21.962959609877934	36.50895336427267
10-11	22.820001110030738	27.659784218945187	30.237679125179078	19.282535545844993
12-13	24.157629615053633	25.639432927700557	30.16189077052063	20.04104668672518
14-15	21.33967093857308	26.65977698362308	28.206231534385157	23.794320543418685
16-17	20.220878535176144	29.638209615950466	28.878237041273625	21.262674807599762
18-19	20.186080483388803	28.65637270399311	31.07553609212543	20.082010720492658
20-21	21.383508291671944	27.659885123082745	30.895485463145754	20.061121122099557
22-23	20.97459460848184	27.64577932414912	30.73292726671868	20.64669880065036
24-25	20.592418279619253	27.836313537390478	30.889688595231252	20.681579587759018
26-27	20.591136733738484	28.010675731949696	30.663637704236475	20.734549830075345
28-29	20.524585256935985	28.104306937244832	30.744344488651144	20.62676331716804
30-31	20.9615905866729	27.893166607263836	30.571177758808442	20.57406504725482
32-33	20.748363426555237	28.087871425537703	30.404596407249112	20.759168740657945
34-35	20.848161165251295	28.188732568906822	30.42554357242603	20.537562693415857
36-37	20.84548938934615	28.101702366530024	30.335246791173354	20.717561452950477
38-39	20.772837348759023	28.185048719589506	30.303918602363332	20.738195329288132
40-41	20.992759685927716	28.088446873538842	30.13759328126734	20.781200159266103
42-43	20.90214564011892	28.219117601875983	29.84632989508026	21.032406862924837
44-45	21.016705171727075	28.470549737737677	29.548761102557243	20.963983987978004
46-47	21.106929962847776	28.48147886626922	29.145857216561588	21.265733954321416
48-49	21.166935919803606	28.567575732573474	29.031617631502815	21.23387071612011
50-51	21.16651767470062	28.684275445066497	28.772289647889	21.37691723234388
52-53	21.236386368934312	28.64577363621127	28.740531863548597	21.377308131305824
54-55	21.23579574487437	28.59989899224743	28.834600380015	21.329704882863197
56-57	21.28274578191757	28.62582924571632	28.759297206769062	21.332127765597043
58-59	21.251785361815053	28.68675338228237	28.79629669518285	21.26516456071973
60-61	21.3355235210079	28.69775041210435	28.752088084647504	21.21463798224024
62-63	21.26797496274705	28.70096982203401	28.732640409211086	21.298414806007848
64-65	21.19037572047725	28.63436321010206	28.758185656210557	21.417075413210142
66-67	21.309499014875932	28.727317047906514	28.59300923362919	21.37017470358836
68-69	21.26940915855074	28.65979179208249	28.680739670331484	21.39005937903529
70-71	21.271412036895555	28.4322850799971	28.76546647298493	21.53083641012242
72-73	21.321068159455663	28.53253434406886	28.75186562013761	21.394531876337865
74-75	21.218133210928457	28.66719625181309	28.6739522530142	21.440718284244255
76-77	21.27481171111073	28.640279974609502	28.63935827236493	21.445550041914842
78-79	21.220944004494424	28.649768784127566	28.631283747347307	21.4980034640307
80-81	21.215649636456853	28.656282568045555	28.650117242760775	21.47795055273681
82-83	21.325390763817555	28.583152479455592	28.76320240201689	21.32825435470996
84-85	21.408706434132405	28.65065197208048	28.60550084402793	21.33514074975918
86-87	21.154764457695826	28.740328740626918	28.707972155562	21.396934646115255
88-89	21.30970755087254	28.6243427526043	28.626457519217098	21.439492177306064
90-91	21.298556968950898	28.635087738132725	28.745375716107937	21.32097957680844
92-93	21.30282817416077	28.640354854398097	28.690123352777707	21.366693618663422
94-95	21.068182267449824	28.75663584645702	28.74663657814346	21.428545307949705
96-97	21.070027264364	28.938154240646124	28.819279612044223	21.17253888294566
98-99	22.020230057467284	30.88127353198168	29.059920944070573	18.038575466480463
100	21.21797096503609	31.856697417945	22.793877253339772	24.131454363679133
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32767.0
1	32480.0
2	40572.0
3	49137.5
4	61532.0
5	102351.5
6	138315.0
7	160344.5
8	169480.0
9	156236.5
10	129987.0
11	103180.0
12	107217.5
13	149896.0
14	261885.0
15	422233.5
16	547001.5
17	607640.5
18	620871.5
19	601382.0
20	560002.5
21	521384.5
22	508377.5
23	543233.5
24	627049.5
25	754100.5
26	921669.0
27	1097211.0
28	1271656.0
29	1465008.0
30	1651822.5
31	1831810.0
32	2027452.5
33	2204720.5
34	2374690.5
35	2535399.0
36	2692265.0
37	2848234.5
38	2932208.5
39	2968757.0
40	3011840.0
41	3033757.5
42	3034155.0
43	3051223.0
44	3062848.5
45	3060983.5
46	3039460.0
47	3001121.5
48	2953123.0
49	2850531.0
50	2721877.5
51	2573556.0
52	2384269.0
53	2183310.5
54	1984599.5
55	1790995.0
56	1610140.0
57	1427811.5
58	1248862.5
59	1089063.5
60	920558.0
61	741411.0
62	580008.5
63	451046.5
64	348136.0
65	265005.0
66	198515.0
67	150641.5
68	116700.0
69	87659.5
70	65406.5
71	49393.0
72	37521.0
73	28370.0
74	20992.5
75	15547.5
76	11430.0
77	8180.5
78	6004.0
79	4507.5
80	3336.5
81	2511.0
82	1988.0
83	1360.0
84	903.5
85	687.0
86	562.5
87	455.5
88	301.5
89	185.0
90	136.0
91	109.5
92	84.5
93	49.5
94	24.5
95	18.0
96	16.5
97	12.0
98	7.0
99	6.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.021880373771698807
4	0.014794458968879218
5	0.07657359544982462
6	0.04600445696047883
7	0.00928615234994485
8	0.004968414040449201
9	0.01507071569085734
10-11	0.021257744408458776
12-13	0.0013329036255848052
14-15	0.00745332221986664
16-17	6.654000740031459E-4
18-19	0.008960113325275238
20-21	0.001969757939914549
22-23	0.0014040778682039706
24-25	0.01101557339366299
26-27	6.810094406045196E-4
28-29	0.0012280798013046112
30-31	0.004510918467299803
32-33	0.02376104960670477
34-35	3.9730055358023233E-4
36-37	8.446272997577089E-5
38-39	8.41546620878377E-4
40-41	0.0033709281889366654
42-43	0.013761656779882834
44-45	9.608817086648162E-4
46-47	0.0015524969366398852
48-49	1.128121953877553E-4
50-51	0.0011143091828100613
52-53	4.797481737816706E-4
54-55	3.377239711724247E-4
56-57	8.391565485836502E-5
58-59	0.009394255546936698
60-61	0.0068984980657675905
62-63	6.20791721548813E-4
64-65	0.010569317871505515
66-67	0.001310548368481965
68-69	0.005167043084461453
70-71	0.005159197656318559
72-73	0.023307387276087842
74-75	5.355065566162251E-4
76-77	0.005691797924730499
78-79	9.064875085975533E-5
80-81	6.493717870649426E-5
82-83	9.401151889296849E-7
84-85	0.0
86-87	0.011884187631494816
88-89	1.6831987281593834E-4
90-91	1.550703285689764E-4
92-93	1.8198736572934242E-5
94-95	1.7202791137410436E-4
96-97	4.117127505897574E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	56843.0
20-21	109326.0
22-23	122199.0
24-25	134477.0
26-27	157938.0
28-29	222357.0
30-31	321816.0
32-33	361550.0
34-35	439446.0
36-37	536233.0
38-39	673581.0
40-41	676105.0
42-43	773533.0
44-45	921531.0
46-47	890601.0
48-49	792191.0
50-51	698626.0
52-53	636856.0
54-55	650140.0
56-57	676663.0
58-59	690532.0
60-61	683657.0
62-63	636812.0
64-65	641955.0
66-67	671972.0
68-69	688346.0
70-71	703260.0
72-73	654591.0
74-75	642364.0
76-77	665067.0
78-79	699734.0
80-81	723193.0
82-83	684685.0
84-85	676784.0
86-87	711450.0
88-89	776467.0
90-91	837335.0
92-93	887634.0
94-95	1034261.0
96-97	2291284.0
98-99	4103359.0
100-101	4.1353728E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.73236142678746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.84161255294225	69.1597758783008
2	7.869349586557671	12.391449516851981
3	1.9760712554045767	4.667422688567964
4	0.824067104799671	2.595229965400562
5	0.44070648942821655	1.7348931304396515
6	0.27662053323461677	1.3067392680419112
7	0.186479068678098	1.0277356203586312
8	0.12013766274284667	0.7566977507231442
9	0.08918924395550917	0.6319871811438361
>10	0.36358767431501415	4.671405519047539
>50	0.008923285713013722	0.4717976067331332
>100	0.003152126581770844	0.41183817402658685
>500	5.368661963487025E-5	0.029281257692708163
>1k	3.482691915618826E-5	0.06306792633826805
>5k	1.4902107926241763E-5	0.08067851633340653
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.2437094170812084E-5	0.0	7.0115919283787765E-6	0.0
3	1.4023183856757553E-6	2.383941255648784E-5	0.0	8.413910314054531E-6	0.0
4	1.4023183856757553E-6	5.8897372198381716E-5	0.0	2.2437094170812084E-5	0.0
5	9.816228699730287E-6	6.450664574108474E-5	0.0	2.383941255648784E-5	0.0
6	1.2620865471081797E-5	6.450664574108474E-5	0.0	2.664404932783935E-5	1.4023183856757553E-6
7	1.2620865471081797E-5	6.731128251243625E-5	0.0	3.0851004484866613E-5	1.4023183856757553E-6
8	1.2620865471081797E-5	7.572519282649077E-5	0.0	3.0851004484866613E-5	4.2069551570272655E-6
9	1.2620865471081797E-5	1.444387937246028E-4	0.0	3.505795964189388E-5	5.609273542703021E-6
10-11	2.944868609919086E-5	1.500480672673058E-4	0.0	3.7862596413245394E-5	5.609273542703021E-6
12-13	3.6460278027569635E-5	1.5285270403865732E-4	0.0	4.06672331845969E-5	5.609273542703021E-6
14-15	4.277071076311053E-5	2.124512354298769E-4	0.0	4.557534753446205E-5	7.011591928378776E-6
16-17	5.7495053812705964E-5	2.2366978251528295E-4	0.0	5.188578027000294E-5	8.413910314054531E-6
18-19	7.011591928378775E-5	2.594289013500147E-4	0.0	6.310432735540899E-5	1.191970627824392E-5
20-21	7.642635201932866E-5	2.951880201847465E-4	0.0	7.50240336336529E-5	1.6126661435271186E-5
22-23	8.203562556203167E-5	3.2113091031974795E-4	0.0	9.39553318402756E-5	1.6827820628109062E-5
24-25	8.834605829757258E-5	3.646027802756964E-4	0.0	1.5145038565298158E-4	1.7528979820946938E-5
26-27	9.535765022595136E-5	3.870398744465084E-4	0.0	2.4610687668609503E-4	1.8230139013784818E-5
28-29	1.065761973113574E-4	3.9264914798921145E-4	0.0	6.100084977689535E-4	1.9632457399460573E-5
30-31	1.290132914821695E-4	4.466384058377281E-4	0.0	0.0017136330672957727	2.1735934977974208E-5
32-33	1.3322024663919673E-4	4.5294883857326893E-4	0.0	0.0036453266435641256	2.2437094170812084E-5
34-35	1.5145038565298158E-4	5.076392556146234E-4	0.0	0.0061176139575104815	2.2437094170812084E-5
36-37	1.724851614381179E-4	5.581227174989505E-4	0.0	0.009795895083137988	2.2437094170812084E-5
38-39	1.8089907175217242E-4	5.833644484411142E-4	0.0	0.015553113215529803	2.734520852067723E-5
40-41	1.977268923802815E-4	6.394571838681444E-4	0.0	0.022047249659594224	2.8046367713515106E-5
42-43	1.9842805157311937E-4	6.618942780389564E-4	0.0	0.027560464392878455	2.8747526906352982E-5
44-45	2.159570313940663E-4	7.38320630058285E-4	0.0	0.033732768767430296	3.225332287054237E-5
46-47	2.2156630493676933E-4	7.90907569521126E-4	0.0	0.040509472366208374	3.3655641256218124E-5
48-49	2.257732600937966E-4	8.014249574136941E-4	0.0	0.04691526275197523	3.3655641256218124E-5
50-51	2.2998021525082386E-4	9.178173834247818E-4	0.0	0.0535047568462656	3.786259641324539E-5
52-53	2.3699180717920264E-4	9.332428856672151E-4	0.0	0.05986427072530515	3.786259641324539E-5
54-55	2.4821035426460865E-4	9.374498408242424E-4	0.0	0.06584235400344089	3.786259641324539E-5
56-57	2.4821035426460865E-4	9.493695471024863E-4	0.0	0.07157292808650487	3.926491479892114E-5
58-59	2.4961267265028445E-4	9.746112780446498E-4	0.0	0.0770195326964695	4.06672331845969E-5
60-61	2.615323789285284E-4	0.0010005541681796515	0.0	0.08250259758446171	4.06672331845969E-5
62-63	2.664404932783935E-4	0.0010117727152650574	0.0	0.08786225845451445	4.06672331845969E-5
64-65	2.664404932783935E-4	0.0010966129775984406	0.0	0.0925929795285916	4.06672331845969E-5
66-67	2.720497668210965E-4	0.0011071303654910088	0.0	0.09724937772822795	4.136839237743478E-5
68-69	2.734520852067723E-4	0.001139383688361551	0.0	0.10193873040992768	4.837998430581355E-5
70-71	2.7695788117096165E-4	0.0011597173049538497	0.0	0.10604752327995765	5.048346188432719E-5
72-73	2.846706322921783E-4	0.0011737404888106072	0.0	0.1092805683181331	5.118462107716507E-5
74-75	2.895787466420434E-4	0.0012221204731164208	0.0	0.11176056838320067	5.469041704135446E-5
76-77	2.9869381614893586E-4	0.00129504102917156	0.0	0.11408841690342242	5.7495053812705964E-5
78-79	3.0360193049880097E-4	0.001324489715270751	0.0	0.11570038188775669	5.7495053812705964E-5
80-81	3.0570540807731464E-4	0.0013293978296206159	0.0	0.11627182662991957	5.889737219838172E-5
82-83	3.064065672701525E-4	0.0013413175358988599	0.0	0.11668410823530824	6.170200896973323E-5
84-85	3.0921120404150406E-4	0.0013658581076481857	0.0	0.11682363891468298	6.170200896973323E-5
86-87	3.2113091031974795E-4	0.001419847365496702	0.0	0.1168720188989888	6.310432735540899E-5
88	3.2814250224812673E-4	0.001500480672673058	0.0	0.1168776281725315	6.450664574108474E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	125640	0.0	50.71681	1
GTATCAA	227120	0.0	35.198013	1
ATCAACG	296440	0.0	26.592194	3
TCAACGC	299785	0.0	26.293951	4
CAACGCA	301295	0.0	26.139042	5
TATCAAC	303675	0.0	26.103968	2
AACGCAG	311750	0.0	25.363237	6
ACGCAGA	352660	0.0	22.283117	7
CGCAGAG	355955	0.0	22.054586	8
GCAGAGT	396700	0.0	19.344995	9
AGAGTAC	369655	0.0	18.275389	10-11
GAGTACT	243380	0.0	18.15007	12-13
GTACATG	259155	0.0	17.791235	1
TACATGG	253950	0.0	17.74656	2
CAGAGTA	389145	0.0	17.679893	10-11
GTGGTAT	49275	0.0	17.636675	1
TGGTATC	46705	0.0	17.305035	2
ACATGGG	255625	0.0	17.223	3
AGTACTT	259110	0.0	17.019876	12-13
GTACTTT	272630	0.0	16.138952	14-15
>>END_MODULE