FastQCFastQC Report
Fri 27 May 2016
SRR2140080_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140080_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30304626
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA519650.049.477911
GTATCAA924500.034.5847471
ATCAACG1182100.026.5473753
TCAACGC1194000.026.2909364
CAACGCA1202400.026.0417025
TATCAAC1222350.025.8255252
AACGCAG1242500.025.307716
ACGCAGA1412650.022.1638837
CGCAGAG1421400.022.0282968
GCAGAGT1608150.019.1254759
GTACATG1075800.018.5024261
TACATGG1061950.018.2606562
AGAGTAC1490850.018.16011610-11
GAGTACT974200.018.04377212-13
ACATGGG1064150.017.772713
GTGGTAT213000.017.5556891
CAGAGTA1578550.017.54781510-11
TGGTATC203150.016.9915492
AGTACTT1035000.016.93621312-13
GTACTTT1087900.016.1048914-15
Produced by FastQC (version 0.11.5)