FastQCFastQC Report
Fri 27 May 2016
SRR2140079_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140079_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6012194
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC151750.064.879021
TATACAC188500.053.6802523
TTATACA189100.052.8749962
GGTATCA85300.051.6918031
GTATCAA162300.037.3204541
TACACAT281650.036.0295035
ATACACA308000.033.5116844
ACACATC314600.031.778976
ATCAACG199300.030.807653
TCAACGC199750.030.7175184
ACATCTC332250.030.1029978
CAACGCA204250.030.0204855
TATCAAC205200.030.0025622
CACATCT338350.029.4746327
AACGCAG210900.029.129886
CATCTCC359750.027.6520379
ACGCAGA239750.025.6243617
TGGTATC32850.025.332932
CGCAGAG243100.025.254228
GTGGTAT38200.023.6273081
Produced by FastQC (version 0.11.5)