##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140079_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6012194
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.452308757834494	32.0	25.0	33.0	18.0	33.0
2	30.255349877266102	32.0	28.0	33.0	25.0	33.0
3	31.20058767232062	33.0	31.0	33.0	28.0	33.0
4	32.195869095375166	33.0	33.0	33.0	31.0	33.0
5	32.78855007007425	33.0	33.0	33.0	32.0	34.0
6	37.03779618555223	38.0	37.0	38.0	36.0	38.0
7	37.09371370917172	38.0	38.0	38.0	36.0	38.0
8	37.41608703910752	38.0	38.0	38.0	37.0	38.0
9	37.47412857935057	38.0	38.0	38.0	37.0	38.0
10-11	37.50404419750926	38.0	38.0	38.0	37.0	38.0
12-13	37.53062076839171	38.0	38.0	38.0	37.5	38.0
14-15	37.51749827101388	38.0	38.0	38.0	38.0	38.0
16-17	37.530070220621624	38.0	38.0	38.0	38.0	38.0
18-19	37.458044933347125	38.0	38.0	38.0	37.0	38.0
20-21	37.53518587571957	38.0	38.0	38.0	37.5	38.0
22-23	37.545536956326785	38.0	38.0	38.0	38.0	38.0
24-25	37.54825226838121	38.0	38.0	38.0	38.0	38.0
26-27	37.524958076892574	38.0	38.0	38.0	38.0	38.0
28-29	37.442880772685115	38.0	38.0	38.0	37.0	38.0
30-31	37.47684012164482	38.0	38.0	38.0	37.0	38.0
32-33	37.44797955929476	38.0	38.0	38.0	37.0	38.0
34-35	37.403492545910915	38.0	38.0	38.0	37.0	38.0
36-37	37.365558694316704	38.0	38.0	38.0	37.0	38.0
38-39	37.307323203553196	38.0	38.0	38.0	37.0	38.0
40-41	37.39530389438405	38.0	38.0	38.0	37.0	38.0
42-43	37.33417077845593	38.0	38.0	38.0	37.0	38.0
44-45	37.333821443568624	38.0	38.0	38.0	37.0	38.0
46-47	37.302430232575304	38.0	38.0	38.0	37.0	38.0
48-49	37.266350260111246	38.0	38.0	38.0	37.0	38.0
50-51	37.2329126578325	38.0	38.0	38.0	37.0	38.0
52-53	37.21213341183191	38.0	38.0	38.0	37.0	38.0
54-55	37.189676842653185	38.0	38.0	38.0	37.0	38.0
56-57	37.19032195185026	38.0	38.0	38.0	37.0	38.0
58-59	37.17961894519098	38.0	38.0	38.0	37.0	38.0
60-61	37.15983524553887	38.0	38.0	38.0	36.5	38.0
62-63	37.15730027788898	38.0	38.0	38.0	36.5	38.0
64-65	37.15135495385003	38.0	38.0	38.0	36.0	38.0
66-67	37.135260658077016	38.0	38.0	38.0	36.0	38.0
68-69	37.12341801871909	38.0	38.0	38.0	36.0	38.0
70-71	37.12164274937143	38.0	38.0	38.0	36.0	38.0
72-73	37.069715909328366	38.0	38.0	38.0	36.0	38.0
74-75	37.077555269053306	38.0	38.0	38.0	36.0	38.0
76-77	37.09269434353965	38.0	38.0	38.0	36.0	38.0
78-79	37.10273303432191	38.0	38.0	38.0	36.0	38.0
80-81	37.110946703733745	38.0	38.0	38.0	36.0	38.0
82-83	37.12552766102364	38.0	38.0	38.0	36.0	38.0
84-85	37.118532821963136	38.0	38.0	38.0	36.0	38.0
86-87	37.107720574557746	38.0	38.0	38.0	36.0	38.0
88-89	37.0965697197171	38.0	38.0	38.0	36.0	38.0
90-91	37.090461600711414	38.0	38.0	38.0	36.0	38.0
92-93	37.088763282987856	38.0	38.0	38.0	36.0	38.0
94-95	37.058015931539146	38.0	38.0	38.0	36.0	38.0
96-97	37.073490217728164	38.0	38.0	38.0	36.0	38.0
98-99	37.06916270895965	38.0	38.0	38.0	36.0	38.0
100	35.43028949600545	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	8.0
18	25.0
19	54.0
20	97.0
21	220.0
22	470.0
23	990.0
24	2099.0
25	4171.0
26	7341.0
27	12148.0
28	19676.0
29	29326.0
30	41127.0
31	54085.0
32	70198.0
33	93868.0
34	141066.0
35	261772.0
36	864282.0
37	4409171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.72642732420145	23.124819325524093	14.259902458237375	24.888850892037084
2	13.070916800118159	20.959280606077137	43.64579057285786	22.32401202094684
3	16.426898400151426	27.84143359312757	32.54312818249045	23.188539824230556
4	11.35051530273308	20.201061376262974	38.48806276044985	29.9603605605541
5	12.08307439054738	38.4479135754332	36.49690998638637	12.972102047633053
6	26.895839834043116	39.25443484242945	19.492315911542	14.357409411985437
7	23.816114383534533	33.666628189309925	23.11818281312945	19.399074614026095
8	22.695444205097346	37.37678572646336	22.872301585994716	17.05546848244458
9	24.005411013838238	16.820831438569225	21.271478474869856	37.902279072722685
10-11	22.833910549127324	27.268414824937455	29.767494195962406	20.130180429972818
12-13	24.212483828698808	24.496473334027478	29.318714599029903	21.97232823824381
14-15	21.404607036965206	26.044019870283623	26.327069618844632	26.22430347390653
16-17	21.061886891873417	29.92603864745549	26.959226531944914	22.052847928726184
18-19	21.3065147265707	27.997516380875272	29.046135237818337	21.64983365473569
20-21	21.759526928622137	27.17542376095205	28.85168882303668	22.21336048738913
22-23	21.34194102341169	28.46032650849289	28.743347997001283	21.45438447109413
24-25	21.61310099245885	28.68864232930191	29.175809931109352	20.522446747129887
26-27	21.7204648662219	28.764497954778616	29.35383681668007	20.161200362319413
28-29	20.943298813788356	27.68666842978706	29.96088569004776	21.40914706637682
30-31	21.428048302214535	29.100658664289508	28.919205830153384	20.552087203342573
32-33	22.891338476369803	27.469885215305993	29.32723826226645	20.311538046057745
34-35	21.377921310432534	27.433022837447872	29.92039929211398	21.268656560005613
36-37	20.662870122987457	28.719782890950896	29.595962149150413	21.021384836911235
38-39	20.76706845747921	27.024739782789737	30.41028558228324	21.797906177447814
40-41	20.97453691111218	26.919790448878288	30.71255011001365	21.39312252999587
42-43	21.858327725537478	26.533216784271634	30.50641521215225	21.102040278038643
44-45	20.723633225474984	27.541610645673227	30.30692401213585	21.427832116715944
46-47	21.964100025563624	26.436046870802464	29.33771585290011	22.2621372507338
48-49	21.444883357596847	26.495650321343607	29.51382132585307	22.545644995206477
50-51	20.732557627924677	26.791372032936668	30.3934046371528	22.082665701985853
52-53	20.949365274977904	27.097176074103274	31.198407377278947	20.75505127363987
54-55	20.927148779144257	27.323801309265004	29.833765417593455	21.91528449399728
56-57	21.949297070937163	28.384823174800395	28.291544143024783	21.37433561123766
58-59	20.26729159893568	29.893567074652267	29.843774056211387	19.995367270200667
60-61	21.35191854566307	31.279555221881477	27.421982470602163	19.946543761853285
62-63	20.179564212323243	32.37892267165407	27.368508641860984	20.073004474161706
64-65	20.184446863486176	32.19006881464128	27.45816068850175	20.167323633370803
66-67	20.38138966176472	31.86659891993015	27.445743726041876	20.306267692263248
68-69	20.590411895700438	31.014590203699026	27.845518068532567	20.549479832067966
70-71	20.7824667516585	30.134952681413687	28.164243531308962	20.918337035618848
72-73	21.235049617376824	28.88122924536196	28.6538348145775	21.229886322683715
74-75	21.245925710190654	28.654948771783584	28.732337309081807	21.36678820894395
76-77	21.312587285091602	28.59605445332714	28.665662282666233	21.425695978915027
78-79	21.376627206580856	28.572035969921256	28.584475988155017	21.466860835342874
80-81	21.39121371162391	28.458450932513045	28.63596182915614	21.514373526706905
82-83	21.46792210044689	28.376805804434298	28.742458769852647	21.41281332526617
84-85	21.666352824969923	28.3042625716151	28.590508750545464	21.43887585286951
86-87	21.436310708983598	28.33006047655259	28.657277708685402	21.576351105778414
88-89	21.586687856062962	28.252099938059693	28.569906246734856	21.59130595914249
90-91	21.63156781369539	28.25778869629548	28.616155796131743	21.494487693877385
92-93	21.64845974079899	28.244664648122004	28.56299060069547	21.543885010383537
94-95	21.552875806394045	28.3567802734775	28.52458066072391	21.565763259404548
96-97	21.644943198452495	28.429891823509667	28.63386444887081	21.291300529167025
98-99	22.652448802544754	30.318817529377746	28.877770183232997	18.1509634848445
100	21.5008137078671	31.28791957634542	22.841845283833443	24.369421431954045
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1287.0
1	1319.0
2	2002.5
3	2883.5
4	3559.0
5	4594.0
6	5534.5
7	6935.0
8	8857.0
9	10168.5
10	10618.0
11	10942.0
12	12076.5
13	14424.0
14	18833.0
15	23623.0
16	26656.5
17	27879.0
18	27150.5
19	25106.5
20	22873.5
21	21829.5
22	23289.5
23	28000.0
24	35577.0
25	46528.5
26	61028.0
27	76497.5
28	91212.0
29	106642.0
30	121794.0
31	135991.5
32	151722.5
33	167290.5
34	181982.5
35	196299.5
36	211109.5
37	223556.5
38	232116.5
39	238935.5
40	243592.0
41	245378.5
42	263477.0
43	336210.0
44	356545.0
45	325066.5
46	300462.0
47	258079.0
48	235491.0
49	222635.5
50	213156.5
51	202738.5
52	189740.5
53	176188.5
54	163022.5
55	148444.0
56	133516.5
57	118966.0
58	104319.5
59	90768.0
60	76601.5
61	62199.0
62	48167.5
63	36750.0
64	28564.0
65	21332.5
66	15430.5
67	11516.0
68	8599.0
69	6141.5
70	4334.5
71	3102.5
72	2175.0
73	1490.0
74	1055.0
75	711.5
76	440.5
77	270.5
78	183.5
79	132.5
80	87.5
81	50.0
82	33.5
83	26.0
84	18.5
85	12.0
86	8.0
87	5.5
88	4.5
89	4.5
90	4.0
91	1.5
92	0.0
93	0.5
94	1.0
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	3.6592298917832657E-4
3	0.0
4	0.0
5	0.009480731992347552
6	4.989858943340817E-4
7	0.0
8	8.316431572234695E-5
9	1.995943577336327E-4
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	7.485667442070916E-5
26-27	2.495541299544813E-5
28-29	6.241340659946047E-4
30-31	2.3329357621971924E-4
32-33	0.0014620337407311237
34-35	3.0181225684726073E-4
36-37	0.0014407448195745151
38-39	8.147037045680691E-4
40-41	7.270325499315264E-4
42-43	1.034356050004564E-4
44-45	6.088446867646298E-5
46-47	4.0423579355045307E-4
48-49	0.0017487177771009757
50-51	0.001756323863611416
52-53	0.001961542202444895
54-55	0.0013221776795252853
56-57	0.003331611693846605
58-59	4.645716282575879E-4
60-61	7.505784614379995E-5
62-63	0.0022011677574464935
64-65	0.002276002900991377
66-67	0.0032889789633196867
68-69	8.467257116729607E-4
70-71	0.005045542121121494
72-73	9.635264397415277E-4
74-75	0.003940435903552574
76-77	2.041281151024723E-4
78-79	0.0010112849615075583
80-81	0.0
82-83	1.114659567920053E-5
84-85	9.471539939341552E-4
86-87	0.0
88-89	0.0
90-91	0.0030853470092118643
92-93	5.914825332250526E-4
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	41.0
20-21	175.0
22-23	387.0
24-25	600.0
26-27	1736.0
28-29	4646.0
30-31	15317.0
32-33	19485.0
34-35	26070.0
36-37	40180.0
38-39	46946.0
40-41	44262.0
42-43	49676.0
44-45	58035.0
46-47	58113.0
48-49	54204.0
50-51	49329.0
52-53	47304.0
54-55	50005.0
56-57	51625.0
58-59	50996.0
60-61	59128.0
62-63	61693.0
64-65	71095.0
66-67	98531.0
68-69	111527.0
70-71	141969.0
72-73	78428.0
74-75	53071.0
76-77	55396.0
78-79	56884.0
80-81	56317.0
82-83	52107.0
84-85	50840.0
86-87	52256.0
88-89	52334.0
90-91	52073.0
92-93	51252.0
94-95	57785.0
96-97	164779.0
98-99	356808.0
100-101	3608789.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.46427624982037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.78668145678242	76.73553747577077
2	7.092196517905687	12.122588848486116
3	1.8183483324452667	4.662114726075075
4	0.6375866096249361	2.1796351255268767
5	0.2794458346740228	1.1941318005721158
6	0.14172886353359537	0.7267652847364974
7	0.08154963261470694	0.4878706230898272
8	0.04659311869733483	0.31856377341517556
9	0.02853226896850547	0.21946407454426758
>10	0.08513343002233768	1.056864104946031
>50	0.0012263431497811852	0.07400617098563438
>100	8.575968657088575E-4	0.14177473149696945
>500	8.999604726018316E-5	0.053429912348890045
>1k	2.999866845353845E-5	0.02725334800573901
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.663286314446939E-5	0.0	0.0	0.0
3	0.0	1.663286314446939E-5	0.0	0.0	0.0
4	0.0	4.989858943340817E-5	0.0	1.663286314446939E-5	0.0
5	0.0	4.989858943340817E-5	0.0	1.663286314446939E-5	0.0
6	0.0	4.989858943340817E-5	0.0	1.663286314446939E-5	0.0
7	0.0	4.989858943340817E-5	0.0	1.663286314446939E-5	0.0
8	0.0	4.989858943340817E-5	0.0	3.326572628893878E-5	0.0
9	0.0	1.3306290515575513E-4	0.0	3.326572628893878E-5	0.0
10-11	0.0	1.3306290515575513E-4	0.0	6.653145257787756E-5	0.0
12-13	0.0	1.3306290515575513E-4	0.0	7.484788415011226E-5	0.0
14-15	0.0	1.663286314446939E-4	0.0	8.316431572234695E-5	0.0
16-17	0.0	1.663286314446939E-4	0.0	8.316431572234695E-5	0.0
18-19	0.0	1.8296149458916328E-4	0.0	9.979717886681634E-5	0.0
20-21	0.0	2.1622722087810207E-4	0.0	1.1643004201128573E-4	0.0
22-23	0.0	2.578093787392755E-4	0.0	1.2474647358352042E-4	0.0
24-25	0.0	3.3265726288938776E-4	0.0	1.8296149458916328E-4	0.0
26-27	0.0	3.6592298917832657E-4	0.0	2.6612581031151025E-4	0.0
28-29	0.0	3.6592298917832657E-4	0.0	6.486816626343063E-4	0.0
30-31	0.0	3.8255585232279597E-4	0.0	0.0017464506301692862	0.0
32-33	0.0	3.8255585232279597E-4	0.0	0.004831846743468357	0.0
34-35	0.0	4.2413801018396946E-4	0.0	0.008349697298523634	0.0
36-37	0.0	4.6572016804514294E-4	0.0	0.012898785368536012	0.0
38-39	0.0	4.823530311896123E-4	0.0	0.01985963859449645	0.0
40-41	0.0	5.239351890507858E-4	0.0	0.02729452842007427	0.0
42-43	0.0	5.322516206230205E-4	0.0	0.03434686239332929	0.0
44-45	0.0	6.070995047731327E-4	0.0	0.042106093050224266	0.0
46-47	0.0	6.81947388923245E-4	0.0	0.05078844761163728	0.0
48-49	0.0	6.81947388923245E-4	0.0	0.058930234120855046	0.0
50-51	0.0	7.651117046455919E-4	0.0	0.06720508353522858	0.0
52-53	0.0	7.651117046455919E-4	0.0	0.07485620058168449	0.0
54-55	0.0	7.651117046455919E-4	0.0	0.08203328102852303	0.0
56-57	0.0	7.817445677900613E-4	0.0	0.08820407325512117	0.0
58-59	0.0	7.90060999362296E-4	0.0	0.09459109270259741	0.0
60-61	0.0	8.066938625067655E-4	0.0	0.1005789234346064	0.0
62-63	0.0	8.316431572234695E-4	0.0	0.10645032412460409	0.0
64-65	0.0	9.148074729458164E-4	0.0	0.11227182622516838	0.0
66-67	0.0	9.148074729458164E-4	0.0	0.11825965695717737	0.0
68-69	0.0	9.647060623792246E-4	0.0	0.12401462760516377	0.0
70-71	0.0	9.81338925523694E-4	0.0	0.1285304499488872	0.0
72-73	0.0	9.81338925523694E-4	0.0	0.1322478948616761	0.0
74-75	0.0	9.979717886681634E-4	0.0	0.13534992383811967	0.0
76-77	0.0	0.001056186809673806	0.0	0.13801118194123477	0.0
78-79	0.0	0.0010811361043905102	0.0	0.1399821762238544	0.0
80-81	0.0	0.0010811361043905102	0.0	0.1407472879285	0.0
82-83	0.0	0.0010811361043905102	0.0	0.1412545902544063	0.0
84-85	0.0	0.0010811361043905102	0.0	0.14152903249629004	0.0
86-87	0.0	0.0010977689675349797	0.0	0.14164546253830132	0.0
88	0.0	0.0012141990095462655	0.0	0.14164546253830132	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	15175	0.0	64.87902	1
TATACAC	18850	0.0	53.680252	3
TTATACA	18910	0.0	52.874996	2
GGTATCA	8530	0.0	51.691803	1
GTATCAA	16230	0.0	37.320454	1
TACACAT	28165	0.0	36.029503	5
ATACACA	30800	0.0	33.511684	4
ACACATC	31460	0.0	31.77897	6
ATCAACG	19930	0.0	30.80765	3
TCAACGC	19975	0.0	30.717518	4
ACATCTC	33225	0.0	30.102997	8
CAACGCA	20425	0.0	30.020485	5
TATCAAC	20520	0.0	30.002562	2
CACATCT	33835	0.0	29.474632	7
AACGCAG	21090	0.0	29.12988	6
CATCTCC	35975	0.0	27.652037	9
ACGCAGA	23975	0.0	25.624361	7
TGGTATC	3285	0.0	25.33293	2
CGCAGAG	24310	0.0	25.25422	8
GTGGTAT	3820	0.0	23.627308	1
>>END_MODULE