FastQCFastQC Report
Fri 27 May 2016
SRR2140078_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140078_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43041742
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA816500.052.7625431
GTATCAA1483700.037.7495461
ATCAACG1818600.030.226773
TCAACGC1851000.029.7158264
TATCAAC1870650.029.6917762
CAACGCA1851400.029.6640475
AACGCAG1933250.028.5449586
ACGCAGA2198750.025.0102637
CGCAGAG2223950.024.7438588
GCAGAGT2522100.021.6572919
TACATGG1901350.020.4914952
GTACATG1946750.020.3587131
ACATGGG1911450.020.064163
GAGTACT1409800.019.89952312-13
CAGAGTA2495000.019.20359810-11
GTACTTT1514000.018.55160914-15
AGAGTAC2371450.018.09019710-11
CATGGGG1122500.017.8424684
GTGGTAT304850.017.2996751
AGTACTT1510700.017.20307512-13
Produced by FastQC (version 0.11.5)