##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140078_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	43041742
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.87292166288251	31.0	30.0	33.0	28.0	34.0
2	31.559944808925252	33.0	31.0	34.0	28.0	34.0
3	31.622605725390947	33.0	31.0	34.0	28.0	34.0
4	35.05862404453797	37.0	35.0	37.0	32.0	37.0
5	35.09858985261331	37.0	35.0	37.0	32.0	37.0
6	35.12240085450073	37.0	35.0	37.0	32.0	37.0
7	35.23596161605169	37.0	35.0	37.0	32.0	37.0
8	35.40830698720326	37.0	35.0	37.0	33.0	37.0
9	37.288759595278464	39.0	37.0	39.0	34.0	39.0
10-11	37.422351353716124	39.0	37.0	39.0	34.5	39.0
12-13	37.450409953203106	39.0	37.0	39.0	34.5	39.0
14-15	38.72809475276349	40.0	38.0	41.0	34.0	41.0
16-17	38.764408152904224	40.0	38.0	41.0	34.5	41.0
18-19	38.833087401527564	40.0	38.5	41.0	35.0	41.0
20-21	38.86824628936316	40.0	39.0	41.0	35.0	41.0
22-23	38.8291837696528	40.0	38.5	41.0	34.0	41.0
24-25	38.802317122423474	40.0	38.0	41.0	34.0	41.0
26-27	38.70716245347977	40.0	38.0	41.0	34.0	41.0
28-29	38.649287204910024	40.0	38.0	41.0	34.0	41.0
30-31	38.544041873953844	40.0	38.0	41.0	34.0	41.0
32-33	38.44837792246	40.0	38.0	41.0	34.0	41.0
34-35	38.39471128777558	40.0	38.0	41.0	34.0	41.0
36-37	38.33251012533691	40.0	38.0	41.0	34.0	41.0
38-39	38.27321011625304	40.0	38.0	41.0	34.0	41.0
40-41	38.243213459678294	40.0	38.0	41.0	33.5	41.0
42-43	38.1806879312533	40.0	38.0	41.0	33.0	41.0
44-45	38.11982842244446	40.0	38.0	41.0	33.0	41.0
46-47	38.06994050890465	40.0	38.0	41.0	33.0	41.0
48-49	37.986095860458576	40.0	37.0	41.0	33.0	41.0
50-51	37.915370350574776	40.0	37.0	41.0	33.0	41.0
52-53	37.82897478881945	40.0	37.0	41.0	33.0	41.0
54-55	37.7187366622082	40.0	37.0	41.0	33.0	41.0
56-57	37.55175633402776	39.5	36.0	41.0	32.0	41.0
58-59	37.40370378820498	39.0	36.0	41.0	32.0	41.0
60-61	37.241971096214215	39.0	35.5	41.0	32.0	41.0
62-63	37.065128539911534	39.0	35.0	40.5	31.0	41.0
64-65	36.84446769915597	38.5	35.0	40.0	31.0	41.0
66-67	36.59830123682127	38.0	35.0	40.0	31.0	41.0
68-69	36.31729104425828	37.5	35.0	40.0	31.0	41.0
70-71	36.033667387079966	37.0	35.0	39.5	31.0	41.0
72-73	35.70988361532183	36.5	34.5	39.0	30.5	41.0
74-75	35.38051379385743	36.0	34.0	39.0	30.0	40.0
76-77	35.36254941425711	36.0	34.0	38.5	31.0	40.0
78-79	35.10888035438521	35.0	34.0	37.5	31.0	39.0
80-81	34.82768615642449	35.0	34.0	37.0	31.0	39.0
82-83	34.53332293200842	35.0	34.0	36.5	30.0	39.0
84-85	34.28439139522753	35.0	34.0	36.0	30.0	37.5
86-87	34.07882600393877	35.0	34.0	36.0	30.0	37.0
88-89	33.90136437584867	35.0	34.0	35.5	30.0	37.0
90-91	33.77445321905962	35.0	34.0	35.0	30.0	36.0
92-93	33.68724076324059	35.0	34.0	35.0	30.0	36.0
94-95	33.64167402694272	35.0	34.0	35.0	30.0	36.0
96-97	33.64170982165836	35.0	34.0	35.0	30.0	36.0
98-99	33.685754861955836	35.0	34.0	35.0	30.0	35.5
100	33.1924034023115	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	15.0
17	588.0
18	5777.0
19	20296.0
20	41175.0
21	65292.0
22	93777.0
23	129789.0
24	176174.0
25	235937.0
26	306969.0
27	389890.0
28	481281.0
29	586786.0
30	712432.0
31	876860.0
32	1096126.0
33	1425974.0
34	1988425.0
35	2853646.0
36	4476505.0
37	8220314.0
38	1.1851389E7
39	6719994.0
40	286329.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.331792704858465	19.78160177624781	9.731959733414136	28.154645785479595
2	13.82630007013855	22.9802127873722	38.469069289212186	24.724417853277064
3	17.26033532750603	28.320937846799975	29.03566495984294	25.383061865851065
4	11.387006594668032	20.09300181205491	38.62194053391241	29.89805105936465
5	12.213803985907449	39.81011735073362	34.71224747362688	13.26383118973205
6	27.318334074260175	40.68305036263283	18.950186318336794	13.048429244770205
7	25.070603604243253	35.032461069392404	22.956984145983782	16.939951180380557
8	24.626489327499804	37.202713589055016	20.716591814522747	17.454205268922436
9	26.345239465447285	17.01478532165357	20.986885242702304	35.65308997019684
10-11	23.44554734796747	27.966399454743257	28.729320481499098	19.858732715790172
12-13	24.742807220553647	25.80721235230703	29.014863734252756	20.435116692886563
14-15	21.946084078483747	26.591904132786198	27.27945827141615	24.182553517313902
16-17	20.627449340390466	29.610092570407964	28.065475085854512	21.696983003347057
18-19	20.590547891858094	28.66690316576871	30.19635566794671	20.546193274426486
20-21	21.88512443166509	27.610711064119315	30.041892588955537	20.462271915260057
22-23	21.530253717593194	27.540716718467923	29.931288501982095	20.997741061956784
24-25	21.033762507764948	27.72911045759921	30.158911724416747	21.078215310219093
26-27	20.94637223018774	27.952217246163297	29.972519081691708	21.128891441957254
28-29	20.830637260048114	27.93303060750107	30.18534912743162	21.05098300501919
30-31	21.276152793558797	27.686495693499293	30.076028966721935	20.961322546219975
32-33	21.033043391040305	27.87344039082581	30.024278128043	21.06923809009088
34-35	21.17923684233658	27.918108623218185	30.128851165255277	20.77380336918996
36-37	21.122785150986935	27.793696302168208	30.03258898818692	21.050929558657934
38-39	20.955407829503	27.900509856127943	30.090477793886635	21.053604520482423
40-41	21.174897736957238	27.755748219345485	29.98895248295496	21.08040156074232
42-43	21.180461480023062	27.831748505948212	29.704247813591973	21.283542200436752
44-45	21.25084983608218	28.207379393923972	29.34999214924256	21.191778620751286
46-47	21.32754916148804	28.12960399947375	28.946920606421756	21.595926232616453
48-49	21.432629019592973	28.264983720739465	28.806558489532847	21.495828770134707
50-51	21.389039416514034	28.401692547371304	28.60984896244276	21.599419073671893
52-53	21.374771989396073	28.439432571665296	28.581651559730126	21.604143879208507
54-55	21.38902430054768	28.407615740469126	28.598760697476422	21.604599261506774
56-57	21.528358093070977	28.440265522129955	28.437624746056922	21.593751638742145
58-59	21.414148571128273	28.481581412781164	28.582900261186513	21.52136975490405
60-61	21.52632472921805	28.56591298841982	28.42129127947541	21.48647100288672
62-63	21.43773012593641	28.58054321692541	28.431796327879276	21.549930329258903
64-65	21.333642311449218	28.482758501154095	28.482939889420074	21.70065929797662
66-67	21.529403239321486	28.52759932301111	28.266455474259978	21.67654196340743
68-69	21.465785063404166	28.496368025452462	28.364316130806948	21.67353078033642
70-71	21.457301638450016	28.236344164773136	28.45242691684607	21.853927279930783
72-73	21.50812107434006	28.272345431142003	28.46889274432917	21.75064075018876
74-75	21.463035815682048	28.37479566910793	28.415890795618726	21.746277719591305
76-77	21.495383042199148	28.355706293863182	28.31609241902924	21.832818244908434
78-79	21.45823989388983	28.384740208701974	28.332424169521996	21.824595727886194
80-81	21.470207539099405	28.476767477325215	28.308723353783165	21.744301629792215
82-83	21.566835654984256	28.370677120958316	28.45138828767166	21.611098936385773
84-85	21.66743395159448	28.434892945589784	28.270536807362255	21.62713629545348
86-87	21.378480172307473	28.535943407530528	28.414167428194194	21.6714089919678
88-89	21.520022977241656	28.39102538183812	28.360827743249207	21.728123897671015
90-91	21.5830446819468	28.323551950012217	28.435051784105557	21.658351583935424
92-93	21.5759277468197	28.393188540068476	28.414754609307607	21.61612910380422
94-95	21.306786167026832	28.446989886218937	28.566719675547976	21.679504271206255
96-97	21.384582392357494	28.60040677540987	28.615570014158326	21.399440818074307
98-99	22.229900055405437	30.577627288521793	28.90576126106035	18.28671139501242
100	21.450456292341684	31.508894624738932	22.619428552629728	24.421220530289652
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	84258.0
1	74952.0
2	43775.5
3	20614.5
4	19675.5
5	22266.0
6	26779.0
7	36717.0
8	55034.5
9	68285.5
10	67221.5
11	64907.0
12	76045.5
13	105641.0
14	170647.5
15	257995.0
16	328189.5
17	367646.0
18	370970.0
19	342927.5
20	297955.0
21	251209.0
22	222716.0
23	225241.0
24	258045.0
25	316613.0
26	401560.5
27	498716.0
28	591401.0
29	692757.0
30	800560.5
31	909719.0
32	1036174.5
33	1158895.5
34	1277410.5
35	1390686.0
36	1500961.0
37	1603853.0
38	1666833.5
39	1705790.0
40	1742382.0
41	1758409.0
42	1766197.0
43	1795638.5
44	1820126.5
45	1842673.0
46	1859093.0
47	1868323.0
48	1847859.5
49	1779697.0
50	1709902.5
51	1627499.5
52	1518118.0
53	1394562.0
54	1263411.0
55	1132306.0
56	1013595.0
57	896645.0
58	780865.5
59	676636.5
60	562268.0
61	437288.0
62	328061.0
63	248594.0
64	188725.0
65	141293.5
66	105297.0
67	79669.5
68	59885.5
69	43216.0
70	30621.0
71	21377.5
72	15052.0
73	10921.0
74	7722.0
75	5324.0
76	3791.0
77	2544.5
78	1555.0
79	944.5
80	634.0
81	440.0
82	321.5
83	236.0
84	177.0
85	130.5
86	100.5
87	83.0
88	62.0
89	53.0
90	50.5
91	37.0
92	24.0
93	13.5
94	6.5
95	7.5
96	13.0
97	16.5
98	24.0
99	36.5
100	51.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002829811116845596
3	0.0
4	0.0
5	0.0
6	0.0076785925625408
7	6.96997812030935E-5
8	0.0
9	0.0
10-11	0.0
12-13	2.323326040103117E-6
14-15	9.409470462417622E-5
16-17	4.170370241985094E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.6506096617080097E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	9.503898395098902E-4
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.00391644543766164
52-53	0.0
54-55	0.0
56-57	8.422423897666358E-4
58-59	0.0034170935647931853
60-61	0.0036968425453915775
62-63	0.002550756932790845
64-65	0.002881974943666459
66-67	0.0029812436257106788
68-69	0.00148355336706191
70-71	0.0
72-73	0.004075313809634344
74-75	0.0033009703458040463
76-77	0.006297900920996216
78-79	1.4355971590336157E-5
80-81	2.4811530309896013E-4
82-83	0.0034197607065357656
84-85	0.002080151421976241
86-87	1.1237665875065504E-4
88-89	7.122794352848593E-5
90-91	4.436028236348147E-4
92-93	1.5564426171669768E-6
94-95	4.582241903881693E-4
96-97	5.676908890853765E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	12513.0
20-21	32628.0
22-23	44752.0
24-25	42866.0
26-27	50597.0
28-29	79118.0
30-31	118287.0
32-33	130223.0
34-35	174906.0
36-37	259443.0
38-39	346643.0
40-41	357194.0
42-43	402798.0
44-45	488014.0
46-47	465904.0
48-49	406645.0
50-51	344164.0
52-53	303755.0
54-55	311292.0
56-57	335618.0
58-59	357012.0
60-61	356044.0
62-63	326218.0
64-65	330313.0
66-67	342874.0
68-69	359344.0
70-71	365443.0
72-73	329042.0
74-75	315850.0
76-77	334621.0
78-79	362716.0
80-81	381229.0
82-83	362934.0
84-85	350842.0
86-87	368101.0
88-89	407201.0
90-91	445473.0
92-93	466933.0
94-95	550794.0
96-97	1370051.0
98-99	2615628.0
100-101	2.7235719E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.91936649497264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.42418716407468	64.85350172844524
2	9.018378905291117	13.69339226602653
3	2.540970978528299	5.787267209159376
4	1.1293761114458487	3.429660756620979
5	0.6072936327807607	2.305267393857295
6	0.35116024645764654	1.5995918069569792
7	0.23208262196618293	1.2333695943915381
8	0.16378127427573264	0.9947336469402385
9	0.11670419964782104	0.7974098014108845
>10	0.40940820076987483	4.722927716992541
>50	0.005396035706418477	0.272446301228383
>100	0.0011264361172515203	0.13679349758179613
>500	7.524206963586787E-5	0.0431514235040555
>1k	4.4214869461609515E-5	0.06392136717112087
>5k	1.4735999230763194E-5	0.06656548971306178
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.323326040103117E-6	0.0
2	0.0	0.0	0.0	3.2526564561443635E-5	0.0
3	0.0	0.0	0.0	3.2526564561443635E-5	0.0
4	0.0	0.0	0.0	6.272980308278415E-5	0.0
5	4.646652080206234E-6	0.0	0.0	6.96997812030935E-5	0.0
6	6.9699781203093496E-6	0.0	0.0	7.434643328329974E-5	0.0
7	9.293304160412467E-6	0.0	0.0	7.666975932340285E-5	0.0
8	1.1616630200515584E-5	0.0	0.0	7.666975932340285E-5	0.0
9	1.6263282280721818E-5	4.646652080206234E-6	0.0	7.666975932340285E-5	0.0
10-11	1.6263282280721818E-5	4.646652080206234E-6	0.0	8.36397374437122E-5	0.0
12-13	1.8586608320824934E-5	4.646652080206234E-6	0.0	8.596306348381532E-5	0.0
14-15	2.207159738097961E-5	6.9699781203093496E-6	0.0	1.1151964992494959E-4	0.0
16-17	2.555658644113428E-5	9.293304160412467E-6	0.0	1.5682450770696036E-4	0.0
18-19	3.020323852134052E-5	1.0454967180464026E-5	0.0	2.0909934360928049E-4	0.0
20-21	5.459816194242324E-5	1.1616630200515584E-5	0.0	2.3233260401031165E-4	0.0
22-23	5.5759824962474797E-5	1.1616630200515584E-5	0.0	2.706674836720131E-4	0.0
24-25	5.808315100257791E-5	1.3939956240618699E-5	0.0	3.914804377573752E-4	0.0
26-27	5.808315100257791E-5	1.3939956240618699E-5	0.0	5.39011641303923E-4	0.0
28-29	6.156814006273259E-5	1.3939956240618699E-5	0.0	0.0012476260835353737	0.0
30-31	6.272980308278415E-5	1.8586608320824934E-5	0.0	0.0037626265219469976	0.0
32-33	6.621479214293882E-5	1.8586608320824934E-5	0.0	0.008228059171025186	0.0
34-35	7.086144422314506E-5	2.4394923421082726E-5	0.0	0.013714593614728697	0.0
36-37	7.666975932340285E-5	3.136490154139208E-5	0.0	0.022798798431531882	0.0
38-39	7.666975932340285E-5	3.2526564561443635E-5	0.0	0.044796049379228195	0.0
40-41	7.666975932340285E-5	4.0658205701804544E-5	0.0	0.06917238619199009	0.0
42-43	7.666975932340285E-5	4.298153174190766E-5	0.0	0.08508949289273654	0.0
44-45	8.015474838355752E-5	4.6466520802062336E-5	0.0	0.10382014742804788	0.0
46-47	8.131641140360909E-5	4.8789846842165446E-5	0.0	0.12288536091313405	0.0
48-49	8.36397374437122E-5	4.8789846842165446E-5	0.0	0.14072850490112598	0.0
50-51	8.36397374437122E-5	5.808315100257791E-5	0.0	0.15898636258727633	0.0
52-53	8.36397374437122E-5	5.808315100257791E-5	0.0	0.19396635944706886	0.0
54-55	8.36397374437122E-5	5.924481402262947E-5	0.0	0.23126038904280408	1.1616630200515584E-6
56-57	8.828638952391844E-5	6.040647704268103E-5	2.323326040103117E-6	0.268705434831146	2.323326040103117E-6
58-59	8.828638952391844E-5	6.272980308278415E-5	2.323326040103117E-6	0.29546434249803366	2.323326040103117E-6
60-61	8.828638952391844E-5	6.272980308278415E-5	2.323326040103117E-6	0.3099793219335779	2.323326040103117E-6
62-63	9.060971556402156E-5	6.505312912288727E-5	2.323326040103117E-6	0.3247974954173556	2.323326040103117E-6
64-65	9.525636764422779E-5	6.505312912288727E-5	2.323326040103117E-6	0.33138993305614817	2.323326040103117E-6
66-67	9.525636764422779E-5	6.505312912288727E-5	2.323326040103117E-6	0.33618295467688086	2.323326040103117E-6
68-69	9.525636764422779E-5	6.505312912288727E-5	2.323326040103117E-6	0.34110724421887945	2.323326040103117E-6
70-71	9.525636764422779E-5	6.621479214293882E-5	2.323326040103117E-6	0.34548903713051393	2.323326040103117E-6
72-73	9.525636764422779E-5	6.96997812030935E-5	2.323326040103117E-6	0.3490890308296537	2.323326040103117E-6
74-75	9.525636764422779E-5	7.086144422314506E-5	2.323326040103117E-6	0.3518967703491183	2.323326040103117E-6
76-77	1.0222634576453713E-4	7.899308536350597E-5	2.323326040103117E-6	0.3544559139822919	2.323326040103117E-6
78-79	1.0222634576453713E-4	8.36397374437122E-5	2.323326040103117E-6	0.356103152144725	2.323326040103117E-6
80-81	1.0222634576453713E-4	8.36397374437122E-5	2.323326040103117E-6	0.35667933700267057	2.323326040103117E-6
82-83	1.0222634576453713E-4	8.36397374437122E-5	2.323326040103117E-6	0.3572415819043755	2.323326040103117E-6
84-85	1.0222634576453713E-4	8.36397374437122E-5	2.323326040103117E-6	0.3574971477687868	2.323326040103117E-6
86-87	1.0338800878458869E-4	8.596306348381532E-5	2.323326040103117E-6	0.3576075057556918	2.323326040103117E-6
88	1.0454967180464024E-4	9.293304160412467E-5	2.323326040103117E-6	0.357613314070792	2.323326040103117E-6
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	81650	0.0	52.762543	1
GTATCAA	148370	0.0	37.749546	1
ATCAACG	181860	0.0	30.22677	3
TCAACGC	185100	0.0	29.715826	4
TATCAAC	187065	0.0	29.691776	2
CAACGCA	185140	0.0	29.664047	5
AACGCAG	193325	0.0	28.544958	6
ACGCAGA	219875	0.0	25.010263	7
CGCAGAG	222395	0.0	24.743858	8
GCAGAGT	252210	0.0	21.657291	9
TACATGG	190135	0.0	20.491495	2
GTACATG	194675	0.0	20.358713	1
ACATGGG	191145	0.0	20.06416	3
GAGTACT	140980	0.0	19.899523	12-13
CAGAGTA	249500	0.0	19.203598	10-11
GTACTTT	151400	0.0	18.551609	14-15
AGAGTAC	237145	0.0	18.090197	10-11
CATGGGG	112250	0.0	17.842468	4
GTGGTAT	30485	0.0	17.299675	1
AGTACTT	151070	0.0	17.203075	12-13
>>END_MODULE