FastQCFastQC Report
Fri 27 May 2016
SRR2140077_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140077_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5378306
Sequences flagged as poor quality0
Sequence length19-100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104950.050.3578071
GTATCAA201250.034.5467221
ATCAACG267700.028.7310923
TCAACGC273850.028.1006054
TGGTATC42800.028.017042
CAACGCA276550.027.8408585
TATCAAC278600.027.752192
AACGCAG286700.026.8964586
GTGGTAT42500.026.0298231
ACGCAGA323550.023.8079687
CGCAGAG326950.023.5356888
TACATGG320600.021.1066882
GCAGAGT364300.021.056199
ACATGGG328750.020.1165583
GTACATG317400.019.8311921
TTATCAA42800.019.8100281
GAGTACT182750.019.2639112-13
CATGGGG202650.018.9668794
CAGAGTA365700.018.40331310-11
AGAGTAC349400.018.10059410-11
Produced by FastQC (version 0.11.5)