##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140077_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5378306
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.521609778246162	18.0	18.0	30.0	18.0	33.0
2	31.052201566813046	32.0	30.0	33.0	27.0	33.0
3	31.87980825189195	33.0	31.0	33.0	29.0	33.0
4	32.639325653839705	33.0	33.0	33.0	31.0	34.0
5	33.028792709079774	33.0	33.0	34.0	33.0	34.0
6	37.07478060935915	38.0	38.0	38.0	36.0	38.0
7	37.31327931136681	38.0	38.0	38.0	36.0	38.0
8	37.2674968661136	38.0	38.0	38.0	36.0	38.0
9	37.46889317937656	38.0	38.0	38.0	37.0	38.0
10-11	37.52321028219666	38.0	38.0	38.0	37.5	38.0
12-13	37.55430929366979	38.0	38.0	38.0	38.0	38.0
14-15	37.52814120282483	38.0	38.0	38.0	37.5	38.0
16-17	37.539376338943896	38.0	38.0	38.0	38.0	38.0
18-19	37.49354583022982	38.0	38.0	38.0	37.5	38.0
20-21	37.57207879117567	38.0	38.0	38.0	38.0	38.0
22-23	37.590181180022775	38.0	38.0	38.0	38.0	38.0
24-25	37.600951451437325	38.0	38.0	38.0	38.0	38.0
26-27	37.58208860052116	38.0	38.0	38.0	38.0	38.0
28-29	37.5159442039714	38.0	38.0	38.0	38.0	38.0
30-31	37.55246396250669	38.0	38.0	38.0	38.0	38.0
32-33	37.542970617968784	38.0	38.0	38.0	38.0	38.0
34-35	37.52273811873967	38.0	38.0	38.0	38.0	38.0
36-37	37.50447975600228	38.0	38.0	38.0	38.0	38.0
38-39	37.4909213933844	38.0	38.0	38.0	38.0	38.0
40-41	37.46744591489259	38.0	38.0	38.0	38.0	38.0
42-43	37.39417027496884	38.0	38.0	38.0	37.0	38.0
44-45	37.39079085034735	38.0	38.0	38.0	37.0	38.0
46-47	37.354703098834335	38.0	38.0	38.0	37.0	38.0
48-49	37.31315350538493	38.0	38.0	38.0	37.0	38.0
50-51	37.2819885218488	38.0	38.0	38.0	37.0	38.0
52-53	37.26531046615876	38.0	38.0	38.0	37.0	38.0
54-55	37.25627112686732	38.0	38.0	38.0	37.0	38.0
56-57	37.259514758357426	38.0	38.0	38.0	37.0	38.0
58-59	37.2578131474004	38.0	38.0	38.0	37.0	38.0
60-61	37.26078469672855	38.0	38.0	38.0	37.0	38.0
62-63	37.26665243493764	38.0	38.0	38.0	37.0	38.0
64-65	37.27352825086683	38.0	38.0	38.0	37.0	38.0
66-67	37.28411151031737	38.0	38.0	38.0	37.0	38.0
68-69	37.29394444876817	38.0	38.0	38.0	37.0	38.0
70-71	37.30388989308764	38.0	38.0	38.0	37.0	38.0
72-73	37.28650723219238	38.0	38.0	38.0	37.0	38.0
74-75	37.28623948649093	38.0	38.0	38.0	37.0	38.0
76-77	37.285155025418234	38.0	38.0	38.0	37.0	38.0
78-79	37.27073316273261	38.0	38.0	38.0	37.0	38.0
80-81	37.26190043661839	38.0	38.0	38.0	37.0	38.0
82-83	37.26223993959887	38.0	38.0	38.0	37.0	38.0
84-85	37.24599433517946	38.0	38.0	38.0	37.0	38.0
86-87	37.23008682586381	38.0	38.0	38.0	37.0	38.0
88-89	37.21144112773449	38.0	38.0	38.0	36.5	38.0
90-91	37.197601767123174	38.0	38.0	38.0	36.0	38.0
92-93	37.19049849126387	38.0	38.0	38.0	36.0	38.0
94-95	37.14992547550972	38.0	38.0	38.0	36.0	38.0
96-97	37.16213197657004	38.0	38.0	38.0	36.0	38.0
98-99	37.150539966673485	38.0	38.0	38.0	36.0	38.0
100	35.57433337632292	38.0	36.0	38.0	31.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	8.0
19	11.0
20	40.0
21	121.0
22	323.0
23	788.0
24	1811.0
25	3460.0
26	5950.0
27	9843.0
28	15564.0
29	23048.0
30	32660.0
31	43505.0
32	56761.0
33	73649.0
34	109853.0
35	207301.0
36	810929.0
37	3982678.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.11941901409106	19.355704193848396	20.06862383806351	22.456252953997037
2	13.999907033821096	21.965695479984383	42.69038543777774	21.344012048416786
3	17.504768229996582	27.75297649482941	31.44930764445162	23.292947630722388
4	11.596234948327597	18.92595921466722	40.44156282665954	29.036243010345636
5	12.370336184111927	38.68331887304187	35.54016719394356	13.406177748902648
6	27.286090773157103	38.966782354080834	20.266475230212748	13.480651642549319
7	25.06850670080877	34.13716140360924	23.941664903410107	16.852666992171887
8	23.777412441761403	36.40441804538455	22.37756646795478	17.440603044899266
9	26.182776375950834	16.710097374653213	22.18521134656143	34.92191490283452
10-11	23.46294539581794	27.55673440670724	29.302646595414988	19.67767360205983
12-13	24.663899748359427	25.661992828225095	29.441919072659683	20.232188350755795
14-15	21.89169786918037	26.609307837821056	28.077279351528155	23.421714941470416
16-17	20.400568506143014	29.673348820241912	28.69108786298139	21.234994810633683
18-19	20.340456641924057	28.45127257541687	31.153647635519437	20.054623147139637
20-21	22.05560879930918	27.237793113838865	30.83036540095336	19.87623268589859
22-23	21.596240156286115	27.244035525390725	30.553977922986142	20.605746395337025
24-25	20.867520997996696	27.519631622551614	30.971317963733934	20.641529415717752
26-27	20.75434558511123	27.74808367427263	30.81635573754296	20.681215003073177
28-29	20.614437514926117	27.81615253111085	30.86521306739165	20.70419688657138
30-31	20.885332204234995	27.661723781064325	30.78181938851148	20.6711246261892
32-33	20.692093352649486	27.72754673182823	30.705578123850998	20.874781791671293
34-35	20.834734705438954	27.80939896428642	30.775674752434718	20.58019157783991
36-37	20.760498254079877	27.74816655653629	30.676750542568925	20.814584646814907
38-39	20.634674003432846	27.829738782604608	30.785847555505025	20.74973965845752
40-41	20.84420910463637	27.80671220820424	30.612308581111563	20.736770106047828
42-43	20.849349723573578	27.81169713649152	30.40926886380545	20.92968427612945
44-45	20.887684019052514	28.020485335162054	30.112608456324057	20.979222189461378
46-47	20.922804934314808	28.079470291628745	29.794632135044996	21.20309263901145
48-49	20.949607486556523	28.161198695028162	29.734994323667387	21.15419949474793
50-51	21.0587798880043	28.375337742783106	29.374296724000796	21.1915856452118
52-53	21.161897332592254	28.47942280520963	29.086751162189312	21.2719287000088
54-55	21.211330532555625	28.425891525639912	29.027267379351134	21.335510562453326
56-57	21.264533708843945	28.494597973672537	28.87045925086878	21.37040906661474
58-59	21.17038491351752	28.506184408040795	29.026404565045855	21.297026113395827
60-61	21.266048134338032	28.49828784971683	28.945206227384805	21.29045778856033
62-63	21.211273963611898	28.454987531269644	28.970606171347978	21.363132333770476
64-65	21.127119539723914	28.406184968394022	29.028324795458587	21.438370696423473
66-67	21.22975778260534	28.448218427562683	28.86286110222791	21.45916268760407
68-69	21.271581833899205	28.470101080902598	28.877282513820866	21.38103457137732
70-71	21.27698109814814	28.21381167234897	28.952685037749287	21.556522191753597
72-73	21.27723265817294	28.26942833037845	28.928873084161218	21.524465927287398
74-75	21.242842498076197	28.392968126369816	28.802206461780933	21.561982913773054
76-77	21.28390120505104	28.407776659280305	28.712092206116708	21.596229929551942
78-79	21.33665123704393	28.351317568109618	28.71792570516317	21.59410548968328
80-81	21.284742955052376	28.4631069368742	28.622190813332548	21.62995929474088
82-83	21.448858973739323	28.384380321926734	28.704577932844437	21.462182771489502
84-85	21.559604874884826	28.346027963418692	28.555390350367592	21.538976811328887
86-87	21.3280986210635	28.50752805978528	28.565861589426977	21.59851172972424
88-89	21.469259936787857	28.367830601731747	28.53755864896042	21.62535081251998
90-91	21.484716973147883	28.433233514670263	28.513414518202296	21.568634993979554
92-93	21.501903280557993	28.432363785356763	28.52944799138196	21.536284942703283
94-95	21.425371157428557	28.520739359169433	28.48401330712491	21.5698761762771
96-97	21.37313216674089	28.650264854863877	28.634710288968783	21.34189268942644
98-99	22.429542148455724	30.544269699928243	28.836593619015133	18.189594532600896
100	28.131593746682565	41.68493723959499	30.18346901372245	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2344.0
1	2284.0
2	2637.0
3	3276.0
4	4288.5
5	5499.0
6	6036.0
7	6175.5
8	6017.0
9	5900.0
10	6328.5
11	7862.0
12	10450.0
13	16825.5
14	26869.0
15	34694.0
16	38825.5
17	38737.5
18	35762.5
19	31824.5
20	28121.0
21	26036.5
22	26661.0
23	30712.5
24	37749.5
25	48050.5
26	60750.0
27	74812.0
28	89852.5
29	105480.0
30	120045.5
31	134562.0
32	150306.5
33	165925.0
34	181144.5
35	195778.5
36	209324.0
37	220271.5
38	228121.0
39	233579.5
40	238459.0
41	242824.0
42	245392.0
43	248458.5
44	250493.0
45	249623.0
46	246340.0
47	240681.0
48	233660.0
49	224370.5
50	212719.0
51	198062.0
52	181959.0
53	165149.0
54	147775.5
55	131392.5
56	115402.0
57	100967.0
58	87306.0
59	74032.0
60	62491.0
61	50618.0
62	39980.5
63	30582.5
64	22428.0
65	17206.0
66	13491.0
67	10586.5
68	7466.0
69	5001.5
70	3526.5
71	2574.0
72	1820.0
73	1365.5
74	1055.0
75	724.5
76	440.5
77	258.0
78	168.0
79	121.5
80	96.5
81	82.5
82	63.0
83	41.5
84	23.0
85	14.0
86	10.5
87	8.0
88	4.0
89	2.5
90	2.5
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	1.115592902300464E-4
3	0.0
4	0.0
5	0.006693557413802785
6	3.718643007668214E-4
7	0.0
8	0.0
9	1.3015250526838748E-4
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	1.3961569198470036E-4
26-27	2.793375156987684E-5
28-29	6.522490432205374E-4
30-31	2.706991000281714E-4
32-33	0.0015831123689412463
34-35	3.1054372159171914E-4
36-37	0.001335565590715451
38-39	5.736994925723605E-4
40-41	7.7286695964765E-4
42-43	1.2691066445933227E-4
44-45	6.91898012651454E-5
46-47	4.7120435120123527E-4
48-49	0.0017082088484608275
50-51	0.0018475006876521416
52-53	0.0019464938152252025
54-55	0.0016420055095111316
56-57	0.003381284600470632
58-59	4.6787061745320614E-4
60-61	3.309034723686776E-5
62-63	0.002179898164100945
64-65	0.002593975542775223
66-67	0.0036986453998385004
68-69	9.790160725301727E-4
70-71	0.005488783245583777
72-73	9.96220842236307E-4
74-75	0.00429656838073779
76-77	2.5928550791561614E-4
78-79	0.0011406626297237378
80-81	0.0
82-83	1.2923177525043503E-5
84-85	0.0011146636096033646
86-87	0.0
88-89	1.3515529884458438E-5
90-91	0.0037643536591028806
92-93	5.446293748227336E-4
94-95	1.4196384549961121E-5
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	1060.0
20-21	3051.0
22-23	1869.0
24-25	1913.0
26-27	3028.0
28-29	6630.0
30-31	17991.0
32-33	22748.0
34-35	30655.0
36-37	46433.0
38-39	54591.0
40-41	52564.0
42-43	60196.0
44-45	70241.0
46-47	71142.0
48-49	66122.0
50-51	76098.0
52-53	57190.0
54-55	61161.0
56-57	62787.0
58-59	62061.0
60-61	61475.0
62-63	58392.0
64-65	60353.0
66-67	62938.0
68-69	63111.0
70-71	62186.0
72-73	58131.0
74-75	57795.0
76-77	60303.0
78-79	60844.0
80-81	60012.0
82-83	56466.0
84-85	55852.0
86-87	56868.0
88-89	59192.0
90-91	59880.0
92-93	58338.0
94-95	60583.0
96-97	148355.0
98-99	1048079.0
100-101	2279622.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.68911300579101
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.47005065704748	82.93556823031153
2	5.645727033432181	10.127204998026965
3	1.1649445635045874	3.1344853380493447
4	0.375379925338533	1.3466997017517233
5	0.15286601894120155	0.6855208823781409
6	0.06606904177954209	0.35554042526098073
7	0.04164001719802397	0.26142593456256574
8	0.022678054823453548	0.16271796974497996
9	0.013061557333667935	0.10543315425578657
>10	0.04536437543222146	0.6447289170334161
>50	0.0015343313479309045	0.09547202433989775
>100	6.564048648662036E-4	0.1310793904724712
>500	2.8018956326309614E-5	0.014123033812231452
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	3.718643007668214E-5	0.0
6	0.0	0.0	0.0	3.718643007668214E-5	0.0
7	0.0	0.0	0.0	5.5779645115023205E-5	0.0
8	0.0	0.0	0.0	5.5779645115023205E-5	0.0
9	0.0	0.0	0.0	5.5779645115023205E-5	0.0
10-11	0.0	0.0	0.0	6.507625263419374E-5	0.0
12-13	0.0	0.0	0.0	7.437286015336428E-5	0.0
14-15	0.0	1.859321503834107E-5	0.0	7.437286015336428E-5	0.0
16-17	0.0	1.859321503834107E-5	0.0	8.366946767253481E-5	0.0
18-19	0.0	1.859321503834107E-5	0.0	1.0226268271087587E-4	0.0
20-21	0.0	1.859321503834107E-5	0.0	1.1155929023004641E-4	0.0
22-23	0.0	2.7889822557511603E-5	0.0	1.1155929023004641E-4	0.0
24-25	0.0	3.718643007668214E-5	0.0	1.5804232782589908E-4	0.0
26-27	0.0	3.718643007668214E-5	0.0	2.0452536542175175E-4	0.0
28-29	0.0	3.718643007668214E-5	0.0	6.972455639377901E-4	0.0
30-31	0.0	3.718643007668214E-5	0.0	0.002138219729409223	0.0
32-33	0.0	3.718643007668214E-5	0.0	0.005280473070888863	0.0
34-35	0.0	3.718643007668214E-5	0.0	0.009315200734208877	0.0
36-37	0.0	4.648303759585267E-5	0.0	0.015014021143460413	0.0
38-39	0.0	5.5779645115023205E-5	0.0	0.02402243382953666	0.0
40-41	0.0	6.507625263419374E-5	0.0	0.03335622777878388	0.0
42-43	0.0	7.437286015336428E-5	0.0	0.04211363206184252	0.0
44-45	0.0	7.437286015336428E-5	0.0	0.0524049765855643	0.0
46-47	0.0	7.437286015336428E-5	0.0	0.06356090560856895	0.0
48-49	0.0	7.437286015336428E-5	0.0	0.07427989407817256	0.0
50-51	9.296607519170535E-6	7.437286015336428E-5	0.0	0.08519411130567878	0.0
52-53	1.859321503834107E-5	7.437286015336428E-5	0.0	0.09551334565195807	0.0
54-55	1.859321503834107E-5	7.437286015336428E-5	0.0	0.10557227498770058	0.0
56-57	1.859321503834107E-5	7.437286015336428E-5	0.0	0.11417163694293334	0.0
58-59	1.859321503834107E-5	7.437286015336428E-5	0.0	0.12301271069366451	0.0
60-61	1.859321503834107E-5	7.437286015336428E-5	0.0	0.13168644550905062	0.0
62-63	1.859321503834107E-5	7.437286015336428E-5	0.0	0.14008128209886161	0.0
64-65	1.859321503834107E-5	7.437286015336428E-5	0.0	0.1476952036570623	0.0
66-67	1.859321503834107E-5	9.296607519170534E-5	0.0	0.15575536237618315	0.0
68-69	1.859321503834107E-5	9.296607519170534E-5	0.0	0.16328561446671125	0.0
70-71	3.718643007668214E-5	1.2085589774921695E-4	0.0	0.17041611243391508	0.0
72-73	3.718643007668214E-5	1.3015250526838748E-4	0.0	0.17615211927324329	0.0
74-75	3.718643007668214E-5	1.3015250526838748E-4	0.0	0.18076323660275187	0.0
76-77	3.718643007668214E-5	1.3015250526838748E-4	0.0	0.18537435393226045	0.0
78-79	3.718643007668214E-5	1.3015250526838748E-4	0.0	0.18796810743010903	0.0
80-81	3.718643007668214E-5	1.4874572030672856E-4	0.0	0.18885128514443023	0.0
82-83	3.718643007668214E-5	1.6733893534506963E-4	0.0	0.18925103926775455	0.0
84-85	3.718643007668214E-5	1.6733893534506963E-4	0.0	0.1895578273158872	0.0
86-87	3.718643007668214E-5	1.6733893534506963E-4	0.0	0.18969727642867476	0.0
88	3.718643007668214E-5	1.6733893534506963E-4	0.0	0.18970657303619393	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10495	0.0	50.357807	1
GTATCAA	20125	0.0	34.546722	1
ATCAACG	26770	0.0	28.731092	3
TCAACGC	27385	0.0	28.100605	4
TGGTATC	4280	0.0	28.01704	2
CAACGCA	27655	0.0	27.840858	5
TATCAAC	27860	0.0	27.75219	2
AACGCAG	28670	0.0	26.896458	6
GTGGTAT	4250	0.0	26.029823	1
ACGCAGA	32355	0.0	23.807968	7
CGCAGAG	32695	0.0	23.535688	8
TACATGG	32060	0.0	21.106688	2
GCAGAGT	36430	0.0	21.05619	9
ACATGGG	32875	0.0	20.116558	3
GTACATG	31740	0.0	19.831192	1
TTATCAA	4280	0.0	19.810028	1
GAGTACT	18275	0.0	19.26391	12-13
CATGGGG	20265	0.0	18.966879	4
CAGAGTA	36570	0.0	18.403313	10-11
AGAGTAC	34940	0.0	18.100594	10-11
>>END_MODULE