FastQCFastQC Report
Fri 27 May 2016
SRR2140076_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140076_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33897913
Sequences flagged as poor quality0
Sequence length19-100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA835850.053.6906361
GTATCAA1556200.036.100221
ATCAACG2081450.026.7151663
TATCAAC2098600.026.581242
TCAACGC2127000.026.1683714
CAACGCA2126900.026.1142145
AACGCAG2198650.025.361846
TGCGCGA25650.024.98491794
ACGCAGA2501200.022.2236757
CGCAGAG2513450.022.0913338
TGGTATC251650.021.4132442
GTGGTAT274750.020.7556721
GCAGAGT2752800.019.7576629
TACATGG2253050.019.728522
GTACATG2287500.019.7002091
ACATGGG2312200.018.8319383
GAGTACT1413450.018.67431612-13
AGAGTAC2648600.017.96781710-11
CAGAGTA2728350.017.9108110-11
AGTACTT1487200.017.44373112-13
Produced by FastQC (version 0.11.5)