##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140076_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33897913
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.116063398947304	31.0	31.0	34.0	28.0	34.0
2	31.166601879000634	31.0	31.0	34.0	28.0	34.0
3	31.169397390334915	31.0	31.0	34.0	27.0	34.0
4	34.867875848286	37.0	35.0	37.0	32.0	37.0
5	34.60269447856569	37.0	35.0	37.0	30.0	37.0
6	34.66792221102225	37.0	35.0	37.0	30.0	37.0
7	34.518664998638705	37.0	35.0	37.0	30.0	37.0
8	34.504510734923414	37.0	35.0	37.0	30.0	37.0
9	35.90702675412495	39.0	35.0	39.0	30.0	39.0
10-11	35.84938805819698	38.0	35.0	39.0	30.0	39.0
12-13	35.8062875286747	38.0	35.0	39.0	30.0	39.0
14-15	36.81574157677495	39.0	36.0	41.0	30.0	41.0
16-17	36.868331215553006	39.0	36.0	41.0	30.0	41.0
18-19	36.976930703668984	39.0	36.0	41.0	30.0	41.0
20-21	37.05585003118731	39.0	36.0	41.0	30.0	41.0
22-23	37.030523003179184	39.0	36.0	41.0	30.0	41.0
24-25	37.000513029188596	39.0	36.0	41.0	30.0	41.0
26-27	36.91005677117124	39.0	36.0	41.0	30.0	41.0
28-29	36.869631660641616	39.0	35.5	41.0	30.0	41.0
30-31	36.75878757993651	39.0	35.0	41.0	30.0	41.0
32-33	36.640852260287936	39.0	35.0	41.0	30.0	41.0
34-35	36.58985519467869	39.0	35.0	41.0	30.0	41.0
36-37	36.58109766383508	39.0	35.0	41.0	30.0	41.0
38-39	36.54215948786964	39.0	35.0	41.0	30.0	41.0
40-41	36.53181765798078	39.0	35.0	41.0	30.0	41.0
42-43	36.50886571589764	39.0	35.0	41.0	30.0	41.0
44-45	36.490063255938715	39.0	35.0	41.0	30.0	41.0
46-47	36.46522822929781	39.0	35.0	41.0	29.5	41.0
48-49	36.423072235886764	39.0	35.0	40.0	29.0	41.0
50-51	36.38936948118557	39.0	35.0	40.0	29.0	41.0
52-53	36.34147301895587	39.0	35.0	40.0	29.0	41.0
54-55	36.268145883971926	38.0	34.0	40.0	29.0	41.0
56-57	36.18436967816932	38.0	34.0	40.0	29.0	41.0
58-59	36.06000887231036	38.0	34.0	40.0	28.0	41.0
60-61	35.915116688672555	38.0	34.0	40.0	28.0	41.0
62-63	35.76948604388957	38.0	34.0	40.0	28.0	41.0
64-65	35.598491120644475	37.5	34.0	40.0	28.0	41.0
66-67	35.39910590760378	37.0	33.5	40.0	28.0	41.0
68-69	35.17168663455483	36.5	33.0	39.5	28.0	41.0
70-71	34.958592613833616	36.0	33.0	39.0	28.0	41.0
72-73	34.68425241030288	36.0	33.0	39.0	28.0	40.0
74-75	34.41574044492597	35.0	33.0	38.5	28.0	40.0
76-77	34.13271304743242	35.0	32.0	37.5	28.0	39.5
78-79	33.8571694343685	35.0	32.0	37.0	27.5	39.0
80-81	33.60016463495919	35.0	32.0	37.0	27.0	39.0
82-83	33.4745818086289	35.0	32.0	36.0	27.5	39.0
84-85	33.428988668445115	35.0	32.0	36.0	28.0	37.0
86-87	33.259454268528536	35.0	32.0	36.0	28.0	37.0
88-89	33.11347176503644	35.0	32.0	35.0	28.0	37.0
90-91	33.02586639997919	35.0	32.0	35.0	28.0	36.0
92-93	32.96706417515013	35.0	32.0	35.0	28.5	36.0
94-95	32.93988059836311	34.0	32.0	35.0	29.0	36.0
96-97	32.989884707283714	34.0	32.0	35.0	29.0	36.0
98-99	33.09148344944111	34.0	32.0	35.0	29.0	35.0
100	32.70223466679272	34.0	32.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	6.0
13	15.0
14	61.0
15	98.0
16	204.0
17	719.0
18	4504.0
19	21985.0
20	69576.0
21	155345.0
22	256614.0
23	350277.0
24	432370.0
25	509128.0
26	582798.0
27	654930.0
28	741047.0
29	841932.0
30	966480.0
31	1134850.0
32	1356286.0
33	1665217.0
34	2117410.0
35	2711754.0
36	3710585.0
37	5373499.0
38	6376153.0
39	3711399.0
40	152670.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.76930930821611	21.586246327318147	14.772561366831049	22.871882997634692
2	14.197107060838821	23.23337722885772	41.00962498782742	21.55989072247604
3	16.689503963179263	29.513266090040418	31.87226272590865	21.924967220871665
4	11.226851380595349	20.166207956240438	40.276345076048806	28.330595587115415
5	11.196396531087235	40.43754721171236	35.88116710511966	12.484889152080745
6	26.082599300839732	40.89523551708664	20.134822872984152	12.887342309089473
7	23.812342491498427	35.09324007639093	24.602489027824166	16.491928404286476
8	24.316978460661396	36.50090412632262	22.02823916350179	17.1538782495142
9	26.37348267595459	15.654385586216721	21.786025223223795	36.186106514604894
10-11	23.081487266488757	27.830581323432078	29.61056361780839	19.477367792270776
12-13	24.64832051744778	26.15563008656448	29.78843585267381	19.40761354331393
14-15	21.799988945416153	26.691803085084466	28.514703075928367	22.99350489357101
16-17	19.811045077549082	29.772944661684107	29.101941147179133	21.31406911358767
18-19	19.86747209153445	28.4663368798238	31.8230869652277	19.84310406341405
20-21	21.85347943488482	27.12952741580925	31.427136886417056	19.589856262888866
22-23	21.260498329731	27.245794329845076	31.233448962372435	20.26025837805149
24-25	20.37166974621046	27.565810466354822	31.658366194083992	20.40415359335072
26-27	20.309035672602004	27.781495958707858	31.452602091831633	20.456866276858506
28-29	20.194214265445414	27.878925437962515	31.514143743303546	20.412716553288522
30-31	20.535609968086025	27.734052070474075	31.371974214339417	20.358363747100483
32-33	20.376791133260813	27.91088940828731	31.118228100229555	20.59409135822232
34-35	20.58642943600575	28.012633582986613	31.16552224979745	20.235414731210184
36-37	20.500475446842135	27.986254801153343	30.971568281889166	20.541701470115356
38-39	20.4550574602891	27.980789074074647	31.018063568236208	20.54608989740004
40-41	20.64036551598684	27.97631326981986	30.816060330007844	20.56726088418545
42-43	20.65543809003482	28.115778121628082	30.403941581742767	20.824842206594326
44-45	20.731026418575187	28.496843851443153	29.928868597700593	20.843261132281064
46-47	20.841140380016522	28.52382233658014	29.426636856319366	21.208400427083966
48-49	21.015185051711637	28.64483328361794	29.160664502803264	21.179317161867157
50-51	21.036920656417323	28.78801202286137	28.82056434916737	21.354502971553934
52-53	21.05310423466463	28.82787180401288	28.724518802786136	21.394505158536354
54-55	21.13875509915499	28.846162223572126	28.74254725588622	21.272535421386664
56-57	21.252473695232695	28.794541500976962	28.62331842764129	21.32966637614905
58-59	21.11902039553115	28.833028484935657	28.753998313821988	21.293952805711204
60-61	21.222593978342292	28.902345337232727	28.598353916423047	21.276706768001937
62-63	21.17485637231023	28.82167167151565	28.62410104911049	21.379370907063635
64-65	21.04953659375065	28.759354069138322	28.714086304078485	21.47702303303255
66-67	21.263477603218867	28.805296159140326	28.551969312030973	21.379256925609834
68-69	21.223981139630233	28.79541939061682	28.602679793215625	21.37791967653732
70-71	21.146188711265594	28.592472163599282	28.710955692089545	21.550383433045578
72-73	21.1883931199168	28.678848830331383	28.751535823445256	21.381222226306566
74-75	21.18660822337785	28.75431399109527	28.652324642257337	21.40675314326954
76-77	21.14427572623644	28.770348923758082	28.622308483587368	21.463066866418114
78-79	21.127510287610907	28.75008453788988	28.64841146801997	21.473993706479245
80-81	21.095457360428192	28.790529359312185	28.690552864013846	21.423460416245774
82-83	21.283940180218455	28.670864005390918	28.768050468194673	21.277145346195955
84-85	21.346762574985874	28.77347465933493	28.563809898019617	21.315952867659583
86-87	21.053882144736015	28.905265565802317	28.705115808275373	21.33573648118629
88-89	21.227900764095057	28.70843068291625	28.6092283114171	21.45444024157159
90-91	21.279578766371525	28.703489356764344	28.6622637231007	21.354668153763434
92-93	21.242609000510598	28.816650741971362	28.532859975668824	21.407880281849216
94-95	20.918108604559187	28.965575757519325	28.702592264200007	21.41372337372148
96-97	21.00407319050236	29.113195457132996	28.713911434458073	21.16881991790657
98-99	21.856211791653738	31.0762702148259	29.01966143754315	18.047856555977212
100	27.84491200969237	42.297197073237086	29.857890917070545	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11968.0
1	11943.0
2	18695.0
3	26300.0
4	35518.5
5	61932.5
6	83028.5
7	90794.0
8	88486.5
9	75683.5
10	60718.0
11	47948.5
12	52639.5
13	84319.0
14	160717.0
15	267534.5
16	352032.0
17	390644.0
18	395534.5
19	371998.0
20	330826.5
21	291912.5
22	268143.0
23	275196.0
24	312852.5
25	372021.0
26	448852.0
27	529933.0
28	610726.0
29	703277.5
30	798235.0
31	888311.5
32	980857.5
33	1073558.0
34	1162970.5
35	1256022.0
36	1338046.5
37	1406431.5
38	1456725.5
39	1473693.5
40	1483740.5
41	1492433.0
42	1494490.0
43	1505687.5
44	1530229.5
45	1542789.5
46	1536491.5
47	1522620.5
48	1488242.0
49	1433385.5
50	1372777.0
51	1288680.0
52	1181459.5
53	1068585.5
54	945999.0
55	827215.0
56	724142.0
57	626687.5
58	536811.0
59	454070.0
60	371415.5
61	289638.0
62	219375.5
63	168364.0
64	128218.5
65	98264.0
66	76468.0
67	58972.0
68	43451.0
69	31252.5
70	22919.5
71	17037.0
72	12867.0
73	9770.0
74	7290.5
75	5433.0
76	4087.5
77	3052.5
78	2327.5
79	1782.5
80	1333.5
81	1003.0
82	782.0
83	557.5
84	401.0
85	296.5
86	231.5
87	178.0
88	107.0
89	67.0
90	53.5
91	45.5
92	38.0
93	22.0
94	8.0
95	5.5
96	4.0
97	2.5
98	3.5
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.02196300403508617
4	0.014959623030479782
5	0.07832635596179623
6	0.04756930021031088
7	0.009776413078881878
8	0.004879356437076229
9	0.0148121213244013
10-11	0.020608938373285694
12-13	0.0013673408153475409
14-15	0.007291009331459432
16-17	7.419335815747713E-4
18-19	0.009115605435650272
20-21	0.0020542164276352273
22-23	0.001377092450191964
24-25	0.011310271965883965
26-27	7.304989301754083E-4
28-29	0.0012757465033641673
30-31	0.004744358451079073
32-33	0.024077031952922347
34-35	3.7812819404885515E-4
36-37	8.993984563974906E-5
38-39	8.809165326657095E-4
40-41	0.003266346771525701
42-43	0.014126329612357916
44-45	9.407830744951144E-4
46-47	0.0016929626332321227
48-49	1.2105468479557304E-4
50-51	0.0011490906539688892
52-53	4.612350477206592E-4
54-55	3.4227620382776123E-4
56-57	7.351178412277434E-5
58-59	0.009428454234192694
60-61	0.006724973874852427
62-63	5.706044009532484E-4
64-65	0.010999641312032049
66-67	0.0011981152768202358
68-69	0.005187605843358631
70-71	0.0051577113956163415
72-73	0.024761373817353754
74-75	4.883254724464921E-4
76-77	0.006152529069097109
78-79	6.706126190558396E-5
80-81	8.667222249370347E-5
82-83	0.0
84-85	0.0
86-87	0.012749256826100699
88-89	1.7117827868785003E-4
90-91	1.6538500735739788E-4
92-93	2.5072869737187547E-5
94-95	1.8156826938995482E-4
96-97	3.6544432942900256E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	27276.0
20-21	53414.0
22-23	58375.0
24-25	65037.0
26-27	79121.0
28-29	120791.0
30-31	179183.0
32-33	199883.0
34-35	245452.0
36-37	306616.0
38-39	396828.0
40-41	390409.0
42-43	448051.0
44-45	535818.0
46-47	520605.0
48-49	466173.0
50-51	448273.0
52-53	346426.0
54-55	351422.0
56-57	372695.0
58-59	387058.0
60-61	385186.0
62-63	339570.0
64-65	336112.0
66-67	356885.0
68-69	373171.0
70-71	386546.0
72-73	348696.0
74-75	330997.0
76-77	346215.0
78-79	370221.0
80-81	385519.0
82-83	359127.0
84-85	342505.0
86-87	357649.0
88-89	398837.0
90-91	435313.0
92-93	450436.0
94-95	487407.0
96-97	1023575.0
98-99	6249823.0
100-101	1.3835217E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.65385464482179
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.71669946744774	72.44060482883317
2	7.194497794989996	11.74916954389208
3	1.9247962123411928	4.715010904302338
4	0.8868121553097871	2.896465233077059
5	0.439720265249305	1.795242731152462
6	0.2682597605623929	1.3142666097609799
7	0.1582604902311556	0.9045805345747112
8	0.10231598666169422	0.6683595762172405
9	0.07539653428861612	0.5540775886373385
>10	0.23041774064673642	2.726931414809215
>50	0.0022871589307941437	0.12119837749159573
>100	4.883552198813589E-4	0.07133184177360533
>500	3.466433714657234E-5	0.018686404642772964
>1k	1.3413783606139615E-5	0.024074410835463682
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.950034121569667E-6
2	2.950034121569667E-6	0.0	0.0	0.0	2.950034121569667E-6
3	2.950034121569667E-6	0.0	0.0	0.0	2.950034121569667E-6
4	5.900068243139334E-6	0.0	0.0	8.850102364709001E-6	2.950034121569667E-6
5	8.850102364709001E-6	0.0	0.0	1.1800136486278668E-5	2.950034121569667E-6
6	1.1800136486278668E-5	0.0	0.0	1.1800136486278668E-5	2.950034121569667E-6
7	1.4750170607848335E-5	0.0	0.0	1.1800136486278668E-5	5.900068243139334E-6
8	2.065023885098767E-5	0.0	0.0	1.1800136486278668E-5	5.900068243139334E-6
9	2.065023885098767E-5	0.0	0.0	2.950034121569667E-5	5.900068243139334E-6
10-11	2.8025324154911836E-5	0.0	0.0	3.687542651962084E-5	5.900068243139334E-6
12-13	3.5400409458836005E-5	0.0	0.0	4.130047770197534E-5	8.850102364709001E-6
14-15	4.2775494762760177E-5	0.0	0.0	6.1950716552963E-5	8.850102364709001E-6
16-17	5.1625597127469176E-5	0.0	0.0	8.407597246473553E-5	8.850102364709001E-6
18-19	5.9000682431393334E-5	0.0	0.0	8.997604070787485E-5	1.4750170607848337E-5
20-21	7.227583597845685E-5	0.0	0.0	1.0325119425493835E-4	2.065023885098767E-5
22-23	7.375085303924168E-5	0.0	0.0	1.3570156959220468E-4	2.065023885098767E-5
24-25	8.407597246473553E-5	0.0	0.0	2.0945242263144636E-4	2.065023885098767E-5
26-27	8.555098952552035E-5	0.0	0.0	2.817282586099032E-4	2.065023885098767E-5
28-29	8.850102364709001E-5	0.0	0.0	8.127344004924433E-4	2.2125255911772502E-5
30-31	9.292607482944452E-5	0.0	0.0	0.0022980765807027706	2.507529003334217E-5
32-33	1.0325119425493835E-4	0.0	0.0	0.00474807991866638	2.6550307094127005E-5
34-35	1.1062627955886252E-4	0.0	0.0	0.007687788920810552	2.6550307094127005E-5
36-37	1.1210129661964734E-4	1.4750170607848335E-6	0.0	0.012049414369551304	2.6550307094127005E-5
38-39	1.1505133074121701E-4	2.950034121569667E-6	0.0	0.019215047250844025	3.09753582764815E-5
40-41	1.5930184256476203E-4	2.950034121569667E-6	0.0	0.027374841631105727	3.2450375337266336E-5
42-43	1.5930184256476203E-4	2.950034121569667E-6	0.0	0.03430594679973366	3.5400409458836005E-5
44-45	1.7257699611182553E-4	2.950034121569667E-6	0.0	0.0419362100551736	3.8350443580405673E-5
46-47	1.976522861451677E-4	2.950034121569667E-6	0.0	0.05000307836060586	3.8350443580405673E-5
48-49	2.0945242263144636E-4	2.950034121569667E-6	0.0	0.058139272467895	3.8350443580405673E-5
50-51	2.3010266148243403E-4	2.950034121569667E-6	0.0	0.06654096964612541	3.8350443580405673E-5
52-53	2.3600272972557336E-4	2.950034121569667E-6	0.0	0.07453998716676156	3.8350443580405673E-5
54-55	2.4485283209028236E-4	2.950034121569667E-6	0.0	0.08172922032102684	3.8350443580405673E-5
56-57	2.5222791739420655E-4	2.950034121569667E-6	0.0	0.0884449729987802	4.4250511823545004E-5
58-59	2.537029344549914E-4	2.950034121569667E-6	0.0	0.0945028680674235	5.015058006668434E-5
60-61	2.5665296857656103E-4	2.950034121569667E-6	0.0	0.10066991439856489	5.015058006668434E-5
62-63	2.5665296857656103E-4	2.950034121569667E-6	0.0	0.10677205997903175	5.310061418825401E-5
64-65	2.625530368197004E-4	2.950034121569667E-6	0.0	0.11193756972590024	5.605064830982367E-5
66-67	2.7730320742754873E-4	2.950034121569667E-6	0.0	0.11701162841500007	5.605064830982367E-5
68-69	2.832032756706881E-4	2.950034121569667E-6	0.0	0.1223379150214941	6.0475699492178176E-5
70-71	2.891033439138274E-4	2.950034121569667E-6	0.0	0.12733232278931156	6.490075067453267E-5
72-73	2.9057836097461223E-4	2.950034121569667E-6	0.0	0.13119096742032466	6.490075067453267E-5
74-75	3.038535145216757E-4	2.950034121569667E-6	0.0	0.1341837770366571	7.080081891767201E-5
76-77	3.068035486432454E-4	2.950034121569667E-6	0.0	0.136995159554513	7.375085303924168E-5
78-79	3.0975358276481504E-4	2.950034121569667E-6	0.0	0.13889645654586463	7.817590422159619E-5
80-81	3.127036168863847E-4	2.950034121569667E-6	0.0	0.1394643381142668	8.997604070787485E-5
82-83	3.156536510079544E-4	2.950034121569667E-6	0.0	0.13990684323250224	9.735112601179901E-5
84-85	3.1860368512952406E-4	2.950034121569667E-6	0.0	0.14008532029685722	9.735112601179901E-5
86-87	3.1860368512952406E-4	2.950034121569667E-6	0.0	0.14015317108165332	9.735112601179901E-5
88	3.1860368512952406E-4	2.950034121569667E-6	0.0	0.14016202118401802	9.735112601179901E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	83585	0.0	53.690636	1
GTATCAA	155620	0.0	36.10022	1
ATCAACG	208145	0.0	26.715166	3
TATCAAC	209860	0.0	26.58124	2
TCAACGC	212700	0.0	26.168371	4
CAACGCA	212690	0.0	26.114214	5
AACGCAG	219865	0.0	25.36184	6
TGCGCGA	2565	0.0	24.984917	94
ACGCAGA	250120	0.0	22.223675	7
CGCAGAG	251345	0.0	22.091333	8
TGGTATC	25165	0.0	21.413244	2
GTGGTAT	27475	0.0	20.755672	1
GCAGAGT	275280	0.0	19.757662	9
TACATGG	225305	0.0	19.72852	2
GTACATG	228750	0.0	19.700209	1
ACATGGG	231220	0.0	18.831938	3
GAGTACT	141345	0.0	18.674316	12-13
AGAGTAC	264860	0.0	17.967817	10-11
CAGAGTA	272835	0.0	17.91081	10-11
AGTACTT	148720	0.0	17.443731	12-13
>>END_MODULE