FastQCFastQC Report
Fri 27 May 2016
SRR2140075_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140075_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17498158
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA251300.050.0141331
GTATCAA452650.038.5600051
ATCAACG547650.031.1687813
TCAACGC551500.030.9589394
CAACGCA554300.030.7871425
TATCAAC568600.030.3137232
AACGCAG575600.029.6998026
ACGCAGA663600.025.6887597
CGCAGAG674700.025.291748
GTACATG586800.021.4988561
GCAGAGT794200.021.4754439
TACATGG578800.021.2426222
ACATGGG584750.020.5733683
GAGTACT450250.019.74664912-13
CAGAGTA771750.018.81617210-11
CATGGGG405500.018.556834
AGAGTAC719000.018.47126610-11
GTACTTT490250.018.16311614-15
AGTACTT486900.017.15080312-13
GTACTAG54450.016.4708631
Produced by FastQC (version 0.11.5)