##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140075_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	17498158
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36060452763085	33.0	31.0	34.0	28.0	34.0
2	31.872104309493604	33.0	31.0	34.0	30.0	34.0
3	31.893832196508914	33.0	31.0	34.0	30.0	34.0
4	35.32951879849296	37.0	35.0	37.0	33.0	37.0
5	35.261864877434526	37.0	35.0	37.0	33.0	37.0
6	35.28132561153008	37.0	35.0	37.0	33.0	37.0
7	35.403778271975824	37.0	35.0	37.0	33.0	37.0
8	35.56527361337119	37.0	35.0	37.0	33.0	37.0
9	37.48983310128986	39.0	37.0	39.0	35.0	39.0
10-11	37.598094953765994	39.0	37.5	39.0	35.0	39.0
12-13	37.61612165120466	39.0	38.0	39.0	35.0	39.0
14-15	38.98265866041443	40.0	38.5	41.0	36.0	41.0
16-17	39.01244445272468	40.5	39.0	41.0	36.0	41.0
18-19	39.07315401426824	41.0	39.0	41.0	36.0	41.0
20-21	39.100937551168414	41.0	39.0	41.0	36.0	41.0
22-23	39.06953456155758	41.0	39.0	41.0	35.5	41.0
24-25	39.046332301521005	41.0	39.0	41.0	35.0	41.0
26-27	38.96200474921385	41.0	39.0	41.0	35.0	41.0
28-29	38.90545753942362	40.0	39.0	41.0	35.0	41.0
30-31	38.81307116197742	40.0	38.0	41.0	35.0	41.0
32-33	38.73126487730447	40.0	38.0	41.0	35.0	41.0
34-35	38.67905429383506	40.0	38.0	41.0	35.0	41.0
36-37	38.617583532848386	40.0	38.0	41.0	34.5	41.0
38-39	38.557296918955224	40.0	38.0	41.0	34.0	41.0
40-41	38.522483087733804	40.0	38.0	41.0	34.0	41.0
42-43	38.45886316563701	40.0	38.0	41.0	34.0	41.0
44-45	38.393144212105966	40.0	38.0	41.0	34.0	41.0
46-47	38.33634587593503	40.0	38.0	41.0	34.0	41.0
48-49	38.24197518414644	40.0	38.0	41.0	33.0	41.0
50-51	38.15888552650639	40.0	37.5	41.0	33.0	41.0
52-53	38.05013439336216	40.0	37.0	41.0	33.0	41.0
54-55	37.918655359525275	40.0	37.0	41.0	33.0	41.0
56-57	37.733362441110074	39.5	36.5	41.0	33.0	41.0
58-59	37.563000744018325	39.0	36.0	41.0	33.0	41.0
60-61	37.37451065432607	39.0	36.0	41.0	32.0	41.0
62-63	37.16598453456504	39.0	35.0	40.5	32.0	41.0
64-65	36.91414412704355	38.0	35.0	40.0	32.0	41.0
66-67	36.638203665032776	38.0	35.0	40.0	31.5	41.0
68-69	36.330628946678374	37.0	35.0	40.0	31.0	41.0
70-71	36.021094486649815	37.0	35.0	39.0	31.0	41.0
72-73	35.681041767648125	36.0	34.5	39.0	31.0	40.5
74-75	35.34381330845331	36.0	34.0	38.5	31.0	40.0
76-77	35.309687199981994	35.5	34.0	37.5	31.0	39.5
78-79	35.0602798183942	35.0	34.0	37.0	31.0	39.0
80-81	34.79309518911589	35.0	34.0	37.0	31.0	39.0
82-83	34.51977834319908	35.0	34.0	36.0	31.0	38.0
84-85	34.29221845768797	35.0	34.0	36.0	31.0	37.0
86-87	34.10876163236959	35.0	34.0	35.5	31.0	37.0
88-89	33.9490704737175	35.0	34.0	35.0	31.0	36.5
90-91	33.83887742083826	35.0	34.0	35.0	31.0	36.0
92-93	33.76075370214559	35.0	34.0	35.0	31.0	36.0
94-95	33.718115645398576	35.0	34.0	35.0	31.0	36.0
96-97	33.7167402897299	35.0	34.0	35.0	31.0	35.5
98-99	33.75105083826102	35.0	34.0	35.0	31.0	35.0
100	33.26821190467589	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	7.0
17	173.0
18	1917.0
19	7191.0
20	14458.0
21	22386.0
22	31340.0
23	43448.0
24	58707.0
25	78477.0
26	103754.0
27	131853.0
28	165316.0
29	202835.0
30	250416.0
31	311096.0
32	395754.0
33	523102.0
34	746772.0
35	1109722.0
36	1864252.0
37	3654873.0
38	5090180.0
39	2571469.0
40	118660.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.03816435992863	18.991804737390073	9.439142108557942	28.53088879412336
2	14.223739379636163	22.351769253387673	38.26243946681937	25.16205190015679
3	17.670654248292877	27.399043945082674	28.635979855708243	26.294321950916206
4	12.026774475347633	19.157456459131296	38.14371775589179	30.67205130962928
5	13.005580358801193	38.94888822012008	34.45571242413059	13.589818996948136
6	28.624244557611373	39.51261102916311	18.77127488023999	13.091869532985534
7	25.945838938451928	33.91773629921758	22.807597354759302	17.328827407571193
8	24.926749432711716	36.73576955928732	20.677387871340514	17.660093136660443
9	26.51122478148843	16.70518691167379	20.50907872702944	36.274509579808345
10-11	24.028506314778962	27.081436228887632	28.706167243432134	20.183890212901268
12-13	25.218280118398752	24.76062337532899	28.79768544780542	21.22341105846684
14-15	22.40985933502923	25.607113899979357	26.730116276660876	25.252910488330542
16-17	21.695015671999784	28.595544678429402	27.310589855370466	22.39884979420035
18-19	21.720946284746088	27.877825768860927	29.06438209096066	21.336845855432326
20-21	22.721557267915323	27.028354551082955	28.856388163336938	21.393700017664784
22-23	22.38036549638108	26.94819794117918	28.713682298560308	21.957754263879433
24-25	22.059635797505013	27.06971819095017	28.8754854459234	21.995160565621415
26-27	22.070599838598177	27.2211250818321	28.7006780823997	22.007596997170026
28-29	22.003825887031006	27.261753697308965	28.79211997390177	21.94230044175826
30-31	22.35526008238117	27.04392442811061	28.69485663647382	21.905958853034395
32-33	22.097519645859865	27.30464288519646	28.680468365533695	21.917369103409982
34-35	22.18781668568738	27.261171473549872	28.782357960389838	21.76865388037291
36-37	22.226385882019933	27.11240820135073	28.7297249440646	21.93148097256474
38-39	22.050222490261337	27.28712340682895	28.756487294760912	21.906166808148804
40-41	22.30122777129619	27.096712300624198	28.617689480758727	21.984370447320877
42-43	22.172753134848968	27.239426965740044	28.422681698811996	22.165138200598992
44-45	22.283975826713164	27.418562772342277	28.2416182213857	22.05584317955886
46-47	22.38516796989212	27.397503504839303	27.845439210717686	22.371889314550895
48-49	22.40376469694035	27.48590405090342	27.78402294299417	22.326308309162062
50-51	22.34435182208046	27.65640167098527	27.62961148433253	22.36963502260174
52-53	22.46770187268509	27.59845612796296	27.508646899378856	22.4251950999731
54-55	22.442040421607654	27.48850800996337	27.609173402781707	22.46027816564727
56-57	22.425386594891428	27.58929575167618	27.583891302478175	22.401426350954214
58-59	22.424609498721324	27.60480934723247	27.57678108479954	22.393800069246662
60-61	22.481782568557428	27.615160900903142	27.513126637279	22.389929893260433
62-63	22.452631675671704	27.693524669917462	27.490678461016977	22.363165193393858
64-65	22.41341244803821	27.579506895633184	27.52589713073284	22.481183525595767
66-67	22.458447830807067	27.704684528326595	27.3201999314717	22.516667709394632
68-69	22.40701097556717	27.631901614220173	27.45410236983263	22.506985040380027
70-71	22.462096496523316	27.38479478019771	27.466489061317567	22.686619661961412
72-73	22.519782633516357	27.396420597879363	27.454683600562994	22.629113168041282
74-75	22.355961354178056	27.56247990163116	27.47520563133124	22.606353112859544
76-77	22.488207605881254	27.517477291485264	27.34489146181251	22.64942364082097
78-79	22.49682641597626	27.552693867174906	27.314795542306396	22.63568417454244
80-81	22.506601524012524	27.62162149660641	27.283751740238603	22.588025239142468
82-83	22.553524889056842	27.53617420459723	27.399930364259383	22.510370542086545
84-85	22.671172441024385	27.497228662000907	27.31747305268808	22.514125844286635
86-87	22.42691252275794	27.60660302117418	27.455496631045563	22.510987825022315
88-89	22.495320270941395	27.552977849336415	27.374353038091147	22.577348841631046
90-91	22.600972675315827	27.538109919810168	27.389208830310423	22.471708574563586
92-93	22.61275110974231	27.540397939669113	27.37541286840033	22.471438082188243
94-95	22.400293230336064	27.59277782990695	27.48406979881681	22.52285914094017
96-97	22.48858542013152	27.713960769065366	27.50788302876964	22.28957078203347
98-99	23.4816567499624	29.69572550587983	27.71756892868753	19.105048815470234
100	22.522534600061718	30.625919762938924	21.453148184500584	25.398397452498774
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7823.0
1	7107.5
2	4724.0
3	2975.5
4	3083.0
5	4351.0
6	6494.0
7	10168.5
8	16205.0
9	20514.0
10	20971.5
11	21020.0
12	24478.5
13	33061.0
14	52249.5
15	78850.0
16	100865.5
17	113784.0
18	114683.5
19	104780.0
20	89933.0
21	74885.0
22	65238.5
23	65886.5
24	77154.5
25	98328.0
26	128661.0
27	163540.5
28	198458.0
29	236734.0
30	273915.5
31	309261.0
32	350726.0
33	391049.5
34	431253.5
35	470213.0
36	510845.0
37	553166.0
38	583989.5
39	605520.0
40	629636.0
41	650508.5
42	668365.5
43	691435.0
44	709189.5
45	730776.0
46	749756.0
47	761007.0
48	764731.0
49	750467.5
50	734150.0
51	714336.0
52	683546.5
53	645559.5
54	604597.5
55	556854.5
56	505211.0
57	454423.5
58	402923.0
59	353700.5
60	297993.0
61	239944.5
62	187765.0
63	144444.5
64	108959.0
65	79281.5
66	57188.5
67	42434.5
68	31734.5
69	22869.5
70	16646.0
71	11875.5
72	8058.5
73	5367.0
74	3556.5
75	2247.0
76	1464.0
77	947.0
78	584.0
79	376.0
80	252.0
81	174.5
82	128.0
83	83.5
84	58.5
85	42.5
86	30.5
87	27.0
88	25.0
89	22.5
90	15.5
91	9.0
92	10.0
93	7.5
94	3.0
95	3.0
96	4.0
97	4.5
98	3.0
99	4.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0029888860301752903
3	0.0
4	0.0
5	0.0
6	0.007377919435862906
7	8.572330870483625E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.171551885632762E-4
16-17	4.11471881783214E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.982927174175699E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0010381729544088805
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0039960990751928575
52-53	0.0
54-55	0.0
56-57	8.846397661211814E-4
58-59	0.0034527969695742674
60-61	0.0037218222687450003
62-63	0.002443908939825283
64-65	0.002951794115254377
66-67	0.002897943380799096
68-69	0.0014484520282006742
70-71	0.0
72-73	0.004045415734889223
74-75	0.003126315188918593
76-77	0.006380496434457349
78-79	2.0506704256822682E-5
80-81	2.105662270826277E-4
82-83	0.003330991640396885
84-85	0.00203270771146246
86-87	8.895719852859101E-5
88-89	4.316344763865547E-5
90-91	3.4948912335240454E-4
92-93	0.0
94-95	4.040618088859477E-4
96-97	4.998712926816629E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3645.0
20-21	9301.0
22-23	11692.0
24-25	10874.0
26-27	12823.0
28-29	18721.0
30-31	32024.0
32-33	38715.0
34-35	52367.0
36-37	81192.0
38-39	108751.0
40-41	114579.0
42-43	130681.0
44-45	159515.0
46-47	153530.0
48-49	137136.0
50-51	120688.0
52-53	107432.0
54-55	112609.0
56-57	121679.0
58-59	128304.0
60-61	129400.0
62-63	122243.0
64-65	123343.0
66-67	131028.0
68-69	137075.0
70-71	139252.0
72-73	129002.0
74-75	124740.0
76-77	131409.0
78-79	142143.0
80-81	150453.0
82-83	144111.0
84-85	139984.0
86-87	147629.0
88-89	161864.0
90-91	176704.0
92-93	186275.0
94-95	220765.0
96-97	594724.0
98-99	1112421.0
100-101	1.1487335E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.27947563417308
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.80440872830233	68.81808006714245
2	8.38180659836043	13.290104639701338
3	2.425360411791128	5.76843905012048
4	1.0335774001425526	3.2776589724253387
5	0.49942328136899095	1.979700793321584
6	0.2695729131536449	1.2822959519998467
7	0.16399263714261572	0.9100875196372253
8	0.10216719927011282	0.647980958811729
9	0.07023897925400628	0.5011658499903461
>10	0.24191879166720406	2.8819087886742096
>50	0.00549501179161017	0.299718269675818
>100	0.0019305319502639423	0.23712426185902272
>500	6.1445230190063E-5	0.03637484074675421
>1k	4.607057496932129E-5	0.06936003589393076
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.285954898795633E-5	0.0	5.714887246989083E-6	0.0
3	5.714887246989083E-6	2.285954898795633E-5	0.0	1.1429774493978165E-5	0.0
4	5.714887246989083E-6	7.429353421085807E-5	0.0	1.1429774493978165E-5	0.0
5	5.714887246989083E-6	7.429353421085807E-5	0.0	1.1429774493978165E-5	0.0
6	1.0858285769279257E-4	8.000842145784716E-5	0.0	1.1429774493978165E-5	0.0
7	1.0858285769279257E-4	8.000842145784716E-5	0.0	1.1429774493978165E-5	0.0
8	1.1429774493978166E-4	9.143819595182532E-5	0.0	1.1429774493978165E-5	5.714887246989083E-6
9	1.1429774493978166E-4	1.5430195566870525E-4	0.0	2.285954898795633E-5	5.714887246989083E-6
10-11	1.3429985030424346E-4	1.5430195566870525E-4	0.0	2.285954898795633E-5	5.714887246989083E-6
12-13	1.5144451204521068E-4	1.5430195566870525E-4	0.0	2.285954898795633E-5	5.714887246989083E-6
14-15	1.5430195566870525E-4	2.1145082813859607E-4	0.0	2.285954898795633E-5	1.1429774493978165E-5
16-17	2.6574225698499233E-4	2.1716571538558515E-4	0.0	2.8574436234945415E-5	1.1429774493978165E-5
18-19	2.685997006084869E-4	2.4288270799703604E-4	0.0	2.8574436234945415E-5	1.1429774493978165E-5
20-21	2.685997006084869E-4	2.628848133614978E-4	0.0	3.1431879858439956E-5	1.1429774493978165E-5
22-23	2.828869187259596E-4	2.971741368434323E-4	0.0	4.8576541599407206E-5	2.000210536446179E-5
24-25	3.1146135496090503E-4	3.2003368583138865E-4	0.0	1.0001052682230896E-4	3.42893234819345E-5
26-27	3.2003368583138865E-4	3.314634603253668E-4	0.0	1.5430195566870525E-4	3.42893234819345E-5
28-29	3.2003368583138865E-4	3.3432090394886133E-4	0.0	4.4861864888864303E-4	3.42893234819345E-5
30-31	3.400357911958504E-4	3.657527838073013E-4	0.0	0.001688749181485274	3.42893234819345E-5
32-33	3.514655656898286E-4	3.657527838073013E-4	0.0	0.004057569945362249	3.42893234819345E-5
34-35	3.886123327952577E-4	4.4290376164165396E-4	0.0	0.007143609058736353	3.42893234819345E-5
36-37	4.11471881783214E-4	5.114824086055229E-4	0.0	0.012692764575562752	4.000421072892358E-5
38-39	4.11471881783214E-4	5.343419575934794E-4	0.0	0.02782864344921334	4.000421072892358E-5
40-41	4.1718676903020307E-4	6.17207822674821E-4	0.0	0.04510474759686134	4.000421072892358E-5
42-43	4.1718676903020307E-4	6.400673716627773E-4	0.0	0.05693456419812874	4.000421072892358E-5
44-45	4.314739871476758E-4	7.200757931206244E-4	0.0	0.07093318050962849	4.000421072892358E-5
46-47	4.457612052651485E-4	7.743672219670207E-4	0.0	0.08493179682112825	4.571909797591266E-5
48-49	4.543335361356321E-4	7.800821092140099E-4	0.0	0.09776457613424225	5.714887246989083E-5
50-51	4.943377468645557E-4	8.743777487893297E-4	0.0	0.11136600778207625	5.714887246989083E-5
52-53	5.029100777350393E-4	8.743777487893297E-4	0.0	0.1427978876405162	5.714887246989083E-5
54-55	5.029100777350393E-4	8.772351924128243E-4	0.0	0.17811303338328527	6.0006316093385374E-5
56-57	5.057675213585339E-4	8.972372977772861E-4	0.0	0.2133710302535844	6.286375971687991E-5
58-59	5.086249649820284E-4	9.143819595182533E-4	0.0	0.23323312088049497	6.286375971687991E-5
60-61	5.143398522290174E-4	9.200968467652424E-4	0.0	0.2484975847172028	6.8578646963869E-5
62-63	5.48629175710952E-4	9.315266212592205E-4	0.0	0.259644472292455	7.143609058736354E-5
64-65	5.48629175710952E-4	9.315266212592205E-4	0.0	0.26244476704347963	7.715097783435261E-5
66-67	5.829184991928864E-4	9.34384064882715E-4	0.0	0.2645964220919711	8.000842145784716E-5
68-69	6.000631609338538E-4	9.515287266236823E-4	0.0	0.2666337793955227	8.000842145784716E-5
70-71	6.257801535453046E-4	9.658159447411551E-4	0.0	0.2682168031629386	8.000842145784716E-5
72-73	6.514971461567554E-4	9.77245719235133E-4	0.0	0.269594090989463	8.572330870483625E-5
74-75	6.514971461567554E-4	9.91532937352606E-4	0.0	0.2707284961079903	8.572330870483625E-5
76-77	6.629269206507337E-4	0.0010801136896809367	0.0	0.27174003115070744	8.572330870483625E-5
78-79	6.800715823917009E-4	0.0011515497802683003	0.0	0.2724429622820871	8.572330870483625E-5
80-81	6.800715823917009E-4	0.0011544072238917947	0.0	0.27265155566660215	8.572330870483625E-5
82-83	6.829290260151955E-4	0.0011772667728797512	0.0	0.27298587657055107	9.429563957531986E-5
84-85	6.8578646963869E-4	0.001197268878244213	5.714887246989083E-6	0.2731630380752077	1.028679704458035E-4
86-87	6.943588005091737E-4	0.0012172709836086747	5.714887246989083E-6	0.27322875927854806	1.028679704458035E-4
88	7.200757931206244E-4	0.001314424066807489	5.714887246989083E-6	0.27322875927854806	1.028679704458035E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	25130	0.0	50.014133	1
GTATCAA	45265	0.0	38.560005	1
ATCAACG	54765	0.0	31.168781	3
TCAACGC	55150	0.0	30.958939	4
CAACGCA	55430	0.0	30.787142	5
TATCAAC	56860	0.0	30.313723	2
AACGCAG	57560	0.0	29.699802	6
ACGCAGA	66360	0.0	25.688759	7
CGCAGAG	67470	0.0	25.29174	8
GTACATG	58680	0.0	21.498856	1
GCAGAGT	79420	0.0	21.475443	9
TACATGG	57880	0.0	21.242622	2
ACATGGG	58475	0.0	20.573368	3
GAGTACT	45025	0.0	19.746649	12-13
CAGAGTA	77175	0.0	18.816172	10-11
CATGGGG	40550	0.0	18.55683	4
AGAGTAC	71900	0.0	18.471266	10-11
GTACTTT	49025	0.0	18.163116	14-15
AGTACTT	48690	0.0	17.150803	12-13
GTACTAG	5445	0.0	16.470863	1
>>END_MODULE