FastQCFastQC Report
Fri 27 May 2016
SRR2140074_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140074_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20474288
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA313000.052.9620821
GTATCAA573850.038.168631
ATCAACG700950.030.67673
TCAACGC702450.030.6414454
CAACGCA711650.030.2453235
TATCAAC723000.029.9831072
AACGCAG740550.029.1510936
ACGCAGA846750.025.437557
CGCAGAG861150.025.0023168
GTACATG755600.021.9952851
TACATGG749300.021.7093832
GCAGAGT997900.021.4610619
ACATGGG765750.020.8038443
GAGTACT559600.020.2744612-13
CAGAGTA971100.019.18677710-11
CATGGGG513500.018.8061054
GTACTTT609250.018.6037114-15
AGAGTAC900100.018.48808510-11
AGTACTT609200.016.99822412-13
GTGGTAT129450.016.9430831
Produced by FastQC (version 0.11.5)