##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140074_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	20474288
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.307628817177918	33.0	31.0	34.0	28.0	34.0
2	31.820707074160527	33.0	31.0	34.0	30.0	34.0
3	31.850907001015127	33.0	31.0	34.0	30.0	34.0
4	35.30975997797823	37.0	35.0	37.0	33.0	37.0
5	35.26226084150032	37.0	35.0	37.0	33.0	37.0
6	35.2820505406586	37.0	35.0	37.0	33.0	37.0
7	35.3992418197888	37.0	35.0	37.0	33.0	37.0
8	35.559096511683336	37.0	35.0	37.0	33.0	37.0
9	37.46934980107733	39.0	37.0	39.0	35.0	39.0
10-11	37.572281048308	39.0	37.5	39.0	35.0	39.0
12-13	37.58347225554315	39.0	37.0	39.0	35.0	39.0
14-15	38.93741162085831	40.0	38.0	41.0	35.0	41.0
16-17	38.96561968357581	40.0	38.0	41.0	35.5	41.0
18-19	39.02878082988771	40.5	39.0	41.0	36.0	41.0
20-21	39.05809069280808	41.0	39.0	41.0	35.5	41.0
22-23	39.0275497206205	41.0	39.0	41.0	35.0	41.0
24-25	39.00010822610987	41.0	39.0	41.0	35.0	41.0
26-27	38.91538246817842	40.5	39.0	41.0	35.0	41.0
28-29	38.85894004233072	40.0	38.5	41.0	35.0	41.0
30-31	38.76209275197169	40.0	38.0	41.0	35.0	41.0
32-33	38.67669187356976	40.0	38.0	41.0	35.0	41.0
34-35	38.62194312397278	40.0	38.0	41.0	34.0	41.0
36-37	38.5609019714373	40.0	38.0	41.0	34.0	41.0
38-39	38.49574970734184	40.0	38.0	41.0	34.0	41.0
40-41	38.45840391974656	40.0	38.0	41.0	34.0	41.0
42-43	38.39310749723274	40.0	38.0	41.0	34.0	41.0
44-45	38.32524672418256	40.0	38.0	41.0	34.0	41.0
46-47	38.2623122128755	40.0	38.0	41.0	33.0	41.0
48-49	38.164940838292125	40.0	38.0	41.0	33.0	41.0
50-51	38.07894765741689	40.0	37.0	41.0	33.0	41.0
52-53	37.96993991215974	40.0	37.0	41.0	33.0	41.0
54-55	37.835154113854855	40.0	37.0	41.0	33.0	41.0
56-57	37.645758856153876	39.5	36.0	41.0	33.0	41.0
58-59	37.47072112313266	39.0	36.0	41.0	32.5	41.0
60-61	37.277835963165444	39.0	35.5	41.0	32.0	41.0
62-63	37.06794802146082	39.0	35.0	40.5	32.0	41.0
64-65	36.814322107237786	38.0	35.0	40.0	32.0	41.0
66-67	36.536402946559804	37.5	35.0	40.0	31.0	41.0
68-69	36.22935945897436	37.0	35.0	39.5	31.0	41.0
70-71	35.921269075419374	36.5	35.0	39.0	31.0	41.0
72-73	35.58616381839598	36.0	34.5	39.0	31.0	40.5
74-75	35.25434786439931	35.5	34.0	38.5	31.0	40.0
76-77	35.22753264136636	35.0	34.0	37.5	31.0	39.5
78-79	34.98607615384672	35.0	34.0	37.0	31.0	39.0
80-81	34.72457723526598	35.0	34.0	37.0	31.0	39.0
82-83	34.45797044302617	35.0	34.0	36.0	31.0	38.0
84-85	34.23807669684204	35.0	34.0	36.0	31.0	37.0
86-87	34.06014746050789	35.0	34.0	35.5	30.5	37.0
88-89	33.90471905879165	35.0	34.0	35.0	30.0	36.5
90-91	33.79795877042487	35.0	34.0	35.0	30.0	36.0
92-93	33.72325638671684	35.0	34.0	35.0	30.0	36.0
94-95	33.683408006562004	35.0	34.0	35.0	30.0	36.0
96-97	33.68086928942476	35.0	34.0	35.0	30.5	35.5
98-99	33.7188176994964	35.0	34.0	35.0	31.0	35.0
100	33.23964924343523	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	1.0
16	4.0
17	213.0
18	2250.0
19	7887.0
20	16748.0
21	25926.0
22	37080.0
23	50958.0
24	69640.0
25	93914.0
26	125215.0
27	160170.0
28	200616.0
29	247616.0
30	305485.0
31	377500.0
32	479937.0
33	633518.0
34	895271.0
35	1331621.0
36	2215159.0
37	4288795.0
38	5807689.0
39	2959565.0
40	141509.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.18831502223667	18.81641012376108	9.326502587049669	28.66877226695258
2	14.341657191086313	22.111266758101134	38.071304230602415	25.47577182021014
3	17.85077459103828	27.08100032587214	28.500214512954003	26.568010570135574
4	12.105456365564459	18.879372020165	37.5264429219712	31.48872869229934
5	13.11370143860436	38.88837062368176	34.20248850656003	13.795439431153845
6	29.11078544105376	39.079044089183704	18.371498617449035	13.438671852313497
7	26.231292683135297	33.85337453951361	22.39107856077932	17.524254216571773
8	25.190521887745255	36.64990450461574	20.32773496201675	17.831838645622256
9	26.64865317905072	16.566773897094738	20.306952798553972	36.47762012530057
10-11	24.333844966916555	27.013330085031527	28.29361147992057	20.359213468131347
12-13	25.36722888727559	24.752675160181393	28.504895994429695	21.375199958113317
14-15	22.54224912949269	25.5230101510833	26.503348007362217	25.431392712061797
16-17	21.756737216674225	28.559139586427317	27.182682908668397	22.501440288230057
18-19	21.837924229648426	27.674168693924788	29.03871919746367	21.449187878963116
20-21	22.82050578612125	26.83053480400124	28.834161727526254	21.514797682351258
22-23	22.54355695471165	26.767968081784876	28.71862534926314	21.96984961424033
24-25	22.18274492354014	26.89400287823335	28.859142187056207	22.0641100111703
26-27	22.108114478761433	27.06297325647697	28.770951300056673	22.05796096470492
28-29	22.087327514928802	27.03334619506967	28.839006583984077	22.040319706017456
30-31	22.388483505604736	26.894448980929653	28.728707891361317	21.988359622104294
32-33	22.107258039076573	27.11958212225214	28.748968792798955	22.02419104587233
34-35	22.26410944033137	27.06270682392634	28.80425417969763	21.868929556044662
36-37	22.269524915577488	26.948588442040304	28.726572735971995	22.055313906410216
38-39	22.114432616539386	27.09687746293677	28.78257548940094	22.00611443112291
40-41	22.339636665831154	26.943082380183625	28.639999149609935	22.077281804375286
42-43	22.223233609278672	27.06503754774447	28.45105482983483	22.260674013142026
44-45	22.29587272666906	27.228000450807745	28.279102077198864	22.197024745324327
46-47	22.45684582360785	27.239266971875374	27.849553951743605	22.454333252773175
48-49	22.432172499687432	27.359065115492015	27.74842357288475	22.460338811935802
50-51	22.381984561553516	27.541590458235483	27.633406370883684	22.443018609327318
52-53	22.53150243252273	27.450160509020368	27.50701925693564	22.511317801521262
54-55	22.457299920026063	27.35747397562796	27.608822912526147	22.576403191819825
56-57	22.457432212721248	27.479684484575788	27.584836468318553	22.478046834384408
58-59	22.438247739344785	27.490844889923967	27.585238605436157	22.48566876529509
60-61	22.507376202468464	27.474155876230462	27.560974313964472	22.457493607336602
62-63	22.44969630041687	27.605916354467443	27.50610785741871	22.438279487696978
64-65	22.42951211180513	27.520239710951657	27.4732352280608	22.577012949182418
66-67	22.50497402428378	27.618840581289078	27.30008065094427	22.576104743482873
68-69	22.420155328925375	27.568705455115328	27.40669920114459	22.604440014814706
70-71	22.48615920711177	27.287619457035216	27.4563304627821	22.76989087307092
72-73	22.497125603934805	27.327601649763032	27.392357597589378	22.782915148712785
74-75	22.400201285431386	27.49613405183239	27.41887303569096	22.68479162704526
76-77	22.579371466680143	27.437021234693532	27.26418250722078	22.719424791405544
78-79	22.52178485415138	27.484114167171626	27.257632461793623	22.73646851688337
80-81	22.530951257447366	27.55412103626834	27.220359147368356	22.69456855891594
82-83	22.558503086358392	27.50821368114253	27.33771962642948	22.5955636060696
84-85	22.646287102688955	27.496052051601843	27.29505701188957	22.562603833819633
86-87	22.43234351057816	27.591819713483584	27.405799315179095	22.57003746075916
88-89	22.52035750418357	27.526871768779003	27.318493618345336	22.634277108692093
90-91	22.58640917838716	27.514570421575435	27.35926123592713	22.539759164110272
92-93	22.601203617336747	27.52079651726782	27.34656617595087	22.53143368944456
94-95	22.423390458325386	27.530003179331196	27.44677256480099	22.59983379754243
96-97	22.49613295474335	27.65663311250751	27.508502120303447	22.33873181244569
98-99	23.497363857808747	29.61475282800877	27.699034178462988	19.188849135719497
100	22.54729715688498	30.455613391050367	21.493992929031	25.50309652303366
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12426.0
1	10919.5
2	7026.5
3	4458.5
4	4493.0
5	5994.5
6	8763.5
7	13887.0
8	22187.0
9	28106.0
10	28048.0
11	27091.0
12	30876.0
13	40990.0
14	64613.5
15	97550.5
16	124330.5
17	139615.0
18	140980.5
19	130087.5
20	112277.0
21	93119.5
22	81129.0
23	82706.0
24	97356.0
25	121890.5
26	154489.5
27	193133.5
28	230210.0
29	270367.5
30	312124.0
31	351356.5
32	396431.5
33	442855.0
34	490207.0
35	536041.5
36	580856.0
37	624344.5
38	661032.0
39	690191.0
40	719452.5
41	748155.0
42	772038.5
43	798313.5
44	822637.0
45	845131.0
46	861704.0
47	872646.0
48	877328.0
49	869038.5
50	856545.0
51	838215.5
52	807770.0
53	769616.0
54	727655.0
55	677095.0
56	624724.0
57	567709.0
58	506511.0
59	447385.0
60	379362.5
61	310001.5
62	247078.0
63	191819.0
64	144146.0
65	105036.0
66	76782.0
67	57871.0
68	44069.0
69	32227.5
70	23212.5
71	16677.5
72	11694.5
73	7952.5
74	5334.0
75	3488.0
76	2242.0
77	1360.5
78	844.5
79	558.5
80	374.5
81	240.5
82	159.0
83	114.5
84	90.0
85	77.0
86	62.5
87	42.0
88	28.0
89	22.5
90	16.0
91	8.5
92	6.5
93	5.5
94	5.0
95	7.5
96	8.0
97	7.5
98	6.0
99	5.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002969578233929307
3	0.0
4	0.0
5	0.0
6	0.007936783931143295
7	6.349427144914636E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	9.768349453714826E-5
16-17	4.273652886000236E-4
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	4.234514978409604E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	2.465436430324178E-6
36-37	9.801006162382625E-4
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.003938371254840336
52-53	0.0
54-55	0.0
56-57	8.920867803803166E-4
58-59	0.003351607290148265
60-61	0.0036942651247123697
62-63	0.0025223513161137336
64-65	0.0027644043331899425
66-67	0.003086714785151522
68-69	0.0014938502694144867
70-71	0.0
72-73	0.004173089049756892
74-75	0.0033115734276219122
76-77	0.006301227982490886
78-79	2.6552226961827515E-5
80-81	2.414195829133949E-4
82-83	0.0034068129572031097
84-85	0.0020631303901753853
86-87	1.0451609432577514E-4
88-89	7.14794039400877E-5
90-91	4.121342516942886E-4
92-93	0.0
94-95	4.884287983053656E-4
96-97	5.511237214672193E-4
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	4584.0
20-21	11146.0
22-23	13714.0
24-25	13736.0
26-27	16371.0
28-29	23839.0
30-31	43027.0
32-33	51605.0
34-35	70233.0
36-37	107814.0
38-39	138642.0
40-41	144480.0
42-43	164508.0
44-45	198185.0
46-47	193047.0
48-49	171581.0
50-51	145849.0
52-53	127601.0
54-55	133606.0
56-57	148948.0
58-59	157361.0
60-61	159611.0
62-63	147200.0
64-65	149381.0
66-67	158265.0
68-69	165169.0
70-71	166780.0
72-73	153583.0
74-75	148305.0
76-77	156586.0
78-79	169550.0
80-81	177548.0
82-83	170034.0
84-85	164806.0
86-87	172776.0
88-89	190315.0
90-91	207894.0
92-93	218879.0
94-95	260236.0
96-97	694413.0
98-99	1281732.0
100-101	1.3281348E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.82834352742289
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.13053948118178	69.55486637433462
2	8.33545834862873	13.308116650257187
3	2.3134994562517965	5.540484880325234
4	0.9391970889126959	2.998981914147129
5	0.46780200830242263	1.8671929710792063
6	0.2574179216656774	1.232954776850576
7	0.1531526180730416	0.8558143865361273
8	0.1010554235781267	0.6453669654963142
9	0.0685658691880708	0.49261497838214907
>10	0.22478264939508324	2.7608555858688546
>50	0.0061250478443588335	0.3347272257081413
>100	0.0023086057113969206	0.31404428456692735
>500	5.458025247258095E-5	0.03084374181642876
>1k	4.090101433608151E-5	0.0631352646310095
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.768349453714825E-6	0.0
2	0.0	9.768349453714825E-6	0.0	9.768349453714825E-6	0.0
3	0.0	9.768349453714825E-6	0.0	1.953669890742965E-5	0.0
4	0.0	6.837844617600378E-5	0.0	1.953669890742965E-5	4.884174726857412E-6
5	9.768349453714825E-6	7.32626209028612E-5	0.0	1.953669890742965E-5	4.884174726857412E-6
6	9.768349453714825E-6	7.32626209028612E-5	0.0	2.442087363428706E-5	1.4652524180572238E-5
7	9.768349453714825E-6	7.32626209028612E-5	0.0	2.442087363428706E-5	1.4652524180572238E-5
8	9.768349453714825E-6	1.1233601871772049E-4	0.0	2.9305048361144477E-5	1.953669890742965E-5
9	2.442087363428706E-5	2.5886126052344287E-4	0.0	6.837844617600378E-5	2.442087363428706E-5
10-11	3.418922308800189E-5	2.5886126052344287E-4	0.0	1.1233601871772049E-4	2.442087363428706E-5
12-13	3.90733978148593E-5	2.637454352503003E-4	0.0	1.3431480498857885E-4	2.686296099771577E-5
14-15	4.1515485178288006E-5	3.223555319725892E-4	0.0	1.3675689235200756E-4	5.128383463200283E-5
16-17	6.105218408571765E-5	3.3212388142630405E-4	0.0	1.5140941653257978E-4	6.837844617600378E-5
18-19	7.082053353943247E-5	3.565447550605911E-4	0.0	1.8071446489372427E-4	9.035723244686214E-5
20-21	8.303097035657601E-5	3.858498034217356E-4	0.0	2.0513533852801132E-4	9.279931981029084E-5
22-23	8.791514508343343E-5	4.32249463326881E-4	0.0	2.2467203743544098E-4	1.0745184399086308E-4
24-25	9.524140717371955E-5	4.688807737783116E-4	0.0	2.759558720674438E-4	1.3919897971543626E-4
26-27	1.0256766926400566E-4	4.786491232320264E-4	0.0	3.49218492970305E-4	1.5385150389600848E-4
28-29	1.0745184399086308E-4	4.8109121059545514E-4	0.0	8.083309172949018E-4	1.562935912594372E-4
30-31	1.172201934445779E-4	5.372592199543154E-4	0.0	0.0026716435755910046	1.562935912594372E-4
32-33	1.221043681714353E-4	5.39701307317744E-4	0.0	0.006542352046625504	1.611777659862946E-4
34-35	1.2698854289829273E-4	6.15406015584034E-4	0.0	0.011402105899848629	1.611777659862946E-4
36-37	1.3675689235200756E-4	6.935528112137526E-4	0.0	0.01952937264533936	1.7094611544000944E-4
38-39	1.4164106707886497E-4	7.228578595748971E-4	0.0	0.04306865274142866	1.7583029016686685E-4
40-41	1.4408315444229368E-4	7.765837815703286E-4	0.0	0.06899629427895124	1.880407269840104E-4
42-43	1.465252418057224E-4	8.058888299314731E-4	0.0	0.08438877093064237	1.9292490171086778E-4
44-45	1.465252418057224E-4	8.718251887440482E-4	0.0	0.10255057465246167	1.953669890742965E-4
46-47	1.465252418057224E-4	9.328773728297658E-4	0.0	0.12120079584696669	1.978090764377252E-4
48-49	1.538515038960085E-4	9.377615475566232E-4	0.0	0.13823923938160876	2.0513533852801132E-4
50-51	1.6606194071315202E-4	0.0010720763525452022	0.0	0.15593216232964974	2.0513533852801132E-4
52-53	1.6606194071315202E-4	0.0010867288767257744	0.0	0.20524034828463877	2.319982995257271E-4
54-55	1.6850402807658073E-4	0.0011038234882697752	0.0	0.26243159224877566	2.613033478868716E-4
56-57	1.7338820280343815E-4	0.0011282443619040623	0.0	0.3159767997793135	2.686296099771577E-4
58-59	1.7583029016686685E-4	0.0011331285366309196	0.0	0.33832922541677635	2.857242215211586E-4
60-61	1.7583029016686685E-4	0.001138012711357777	0.0	0.35303303343198067	2.979346583383022E-4
62-63	1.904828143474391E-4	0.0011575494102652067	0.0	0.36335329462983035	3.2723970669944663E-4
64-65	2.0269325116458261E-4	0.0011673177597189216	0.0	0.367236213537682	3.2723970669944663E-4
66-67	2.0513533852801132E-4	0.0011673177597189216	0.0	0.37033766448923644	3.3212388142630405E-4
68-69	2.0513533852801132E-4	0.0011941807207166374	0.0	0.3733536423830709	3.565447550605911E-4
70-71	2.0513533852801132E-4	0.0012259278564412106	0.0	0.3758054980959533	3.638710171508773E-4
72-73	2.295562121622984E-4	0.0012503487300754975	0.0	0.3778837144422311	3.90733978148593E-4
74-75	2.3444038688915582E-4	0.0013089588267977867	0.0	0.37965911195544383	4.005023276023078E-4
76-77	2.3444038688915582E-4	0.0013871056224275052	0.0	0.38122693204276503	4.005023276023078E-4
78-79	2.3444038688915582E-4	0.0014261790202423645	0.0	0.38219399863868286	4.005023276023078E-4
80-81	2.3444038688915582E-4	0.0014261790202423645	0.0	0.382531006694836	4.127127644194513E-4
82-83	2.3444038688915582E-4	0.001438389457059508	0.0	0.3829168564982577	4.3957572541716714E-4
84-85	2.393245616160132E-4	0.0014505998938766516	0.0	0.3831195497494223	4.3957572541716714E-4
86-87	2.393245616160132E-4	0.0014750207675109385	0.0	0.3832050228071423	4.3957572541716714E-4
88	2.393245616160132E-4	0.0015531675631406572	0.0	0.38321234906923257	4.3957572541716714E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	31300	0.0	52.962082	1
GTATCAA	57385	0.0	38.16863	1
ATCAACG	70095	0.0	30.6767	3
TCAACGC	70245	0.0	30.641445	4
CAACGCA	71165	0.0	30.245323	5
TATCAAC	72300	0.0	29.983107	2
AACGCAG	74055	0.0	29.151093	6
ACGCAGA	84675	0.0	25.43755	7
CGCAGAG	86115	0.0	25.002316	8
GTACATG	75560	0.0	21.995285	1
TACATGG	74930	0.0	21.709383	2
GCAGAGT	99790	0.0	21.461061	9
ACATGGG	76575	0.0	20.803844	3
GAGTACT	55960	0.0	20.27446	12-13
CAGAGTA	97110	0.0	19.186777	10-11
CATGGGG	51350	0.0	18.806105	4
GTACTTT	60925	0.0	18.60371	14-15
AGAGTAC	90010	0.0	18.488085	10-11
AGTACTT	60920	0.0	16.998224	12-13
GTGGTAT	12945	0.0	16.943083	1
>>END_MODULE