FastQCFastQC Report
Fri 27 May 2016
SRR2140073_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140073_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23162071
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA346050.051.479531
GTATCAA647500.039.7976651
TCAACGC792850.031.7789884
ATCAACG793600.031.7220973
CAACGCA801250.031.419235
TATCAAC819850.030.9425872
AACGCAG828500.030.5041586
ACGCAGA956650.026.3074537
CGCAGAG974700.025.8054358
GTACATG868850.022.2992461
GCAGAGT1133250.022.1536989
TACATGG865450.022.0666872
ACATGGG868650.021.4440463
GAGTACT619250.020.40179412-13
AGACCGA193600.020.1672988-89
CATGGGG611100.019.4957374
ACCGAGG206250.019.2235690-91
CAGAGTA1096650.019.16045610-11
GAGACCG199100.019.07561588-89
GACCGAG203650.019.06221690-91
Produced by FastQC (version 0.11.5)