FastQCFastQC Report
Fri 27 May 2016
SRR2140072_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140072_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18647352
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA254650.052.6803861
GTATCAA468750.038.4036251
ATCTCGT164300.038.1925994
TATCTCG193850.032.1039194
TCAACGC584400.030.2115144
ATCAACG583600.030.1790683
CAACGCA594850.029.6372175
TATCAAC601350.029.5842232
AACGCAG616550.028.6780076
TATGCCG101700.026.45248886-87
CTCGTAT101100.026.01534882-83
TCGTATG106250.025.6680884-85
TCTCGTA103950.025.25097382-83
ACGCAGA710400.024.8077837
CGCAGAG717750.024.5574448
CCGTCTT113850.024.5195890-91
CGTATGC110700.024.44207884-85
GCCGTCT115850.024.29041190-91
ATGCCGT114250.024.23518888-89
GTACATG646750.024.0149041
Produced by FastQC (version 0.11.5)