##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2140072_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18647352
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56444673753142	33.0	31.0	34.0	28.0	34.0
2	32.05624868345919	33.0	31.0	34.0	30.0	34.0
3	32.08525993395738	33.0	31.0	34.0	30.0	34.0
4	35.394680381428955	37.0	35.0	37.0	33.0	37.0
5	35.385039441524995	37.0	35.0	37.0	33.0	37.0
6	35.45549770283738	37.0	35.0	37.0	33.0	37.0
7	35.539900678659365	37.0	35.0	37.0	33.0	37.0
8	35.71670883887428	37.0	35.0	37.0	35.0	37.0
9	37.66821262343308	39.0	38.0	39.0	35.0	39.0
10-11	37.79929951984603	39.0	38.0	39.0	35.0	39.0
12-13	37.82796396507129	39.0	38.0	39.0	35.0	39.0
14-15	39.24349382153562	41.0	39.0	41.0	36.0	41.0
16-17	39.272905611477704	41.0	39.0	41.0	36.0	41.0
18-19	39.311048399794245	41.0	39.0	41.0	36.0	41.0
20-21	39.327316306224716	41.0	39.0	41.0	36.0	41.0
22-23	39.28682967165682	41.0	39.0	41.0	36.0	41.0
24-25	39.26822646391231	41.0	39.0	41.0	36.0	41.0
26-27	39.19060713774036	41.0	39.0	41.0	36.0	41.0
28-29	39.128183555457184	41.0	39.0	41.0	36.0	41.0
30-31	39.04795547681144	41.0	39.0	41.0	35.0	41.0
32-33	38.97126302611507	41.0	39.0	41.0	35.0	41.0
34-35	38.900070281963664	40.0	38.0	41.0	35.0	41.0
36-37	38.84576654792389	40.0	38.0	41.0	35.0	41.0
38-39	38.801227884132174	40.0	38.0	41.0	35.0	41.0
40-41	38.75611111282204	40.0	38.0	41.0	35.0	41.0
42-43	38.68726859668587	40.0	38.0	41.0	35.0	41.0
44-45	38.61352637624592	40.0	38.0	41.0	34.5	41.0
46-47	38.54035718207185	40.0	38.0	41.0	34.0	41.0
48-49	38.45865049063016	40.0	38.0	41.0	34.0	41.0
50-51	38.33981354897105	40.0	38.0	41.0	34.0	41.0
52-53	38.22695070468512	40.0	37.5	41.0	34.0	41.0
54-55	38.08270640405455	40.0	37.0	41.0	33.0	41.0
56-57	37.913418552732466	40.0	37.0	41.0	33.0	41.0
58-59	37.71688324870067	39.0	36.0	41.0	33.0	41.0
60-61	37.50605863738986	39.0	36.0	41.0	33.0	41.0
62-63	37.273725930717696	39.0	35.0	41.0	33.0	41.0
64-65	37.272861473665735	39.0	35.0	41.0	33.0	41.0
66-67	37.05486345724577	38.0	35.0	40.0	33.0	41.0
68-69	36.76519258854478	37.0	35.0	40.0	33.0	41.0
70-71	36.45706872129057	37.0	35.0	39.5	32.0	41.0
72-73	36.13901576974136	36.5	35.0	39.0	32.0	41.0
74-75	35.796415393580254	36.0	35.0	39.0	32.0	40.5
76-77	35.459940040724575	35.5	35.0	37.5	32.0	39.5
78-79	35.155977681432496	35.0	35.0	37.0	31.5	39.0
80-81	34.863511408732975	35.0	34.5	37.0	31.0	39.0
82-83	34.61034620747997	35.0	34.0	36.0	31.0	38.0
84-85	34.39613334340888	35.0	34.0	36.0	31.0	37.0
86-87	34.202080866689826	35.0	34.0	35.5	31.0	37.0
88-89	34.064738567388346	35.0	34.0	35.0	31.0	36.0
90-91	33.95869732411623	35.0	34.0	35.0	31.0	36.0
92-93	33.88266069571765	35.0	34.0	35.0	31.0	36.0
94-95	33.82078184101244	35.0	34.0	35.0	31.0	36.0
96-97	33.8084292312262	35.0	34.0	35.0	31.0	35.5
98-99	33.85356911871586	35.0	34.0	35.0	31.0	35.0
100	33.256047509343325	35.0	33.0	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	185.0
18	1814.0
19	6674.0
20	13747.0
21	21703.0
22	30546.0
23	41773.0
24	56032.0
25	74378.0
26	97171.0
27	123845.0
28	154655.0
29	189375.0
30	232686.0
31	289888.0
32	367328.0
33	486154.0
34	697931.0
35	1065089.0
36	1865310.0
37	3955397.0
38	5801097.0
39	2923969.0
40	150603.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.47442467970787	18.7799318637842	10.023315911020504	27.722327545487424
2	14.612673138717721	21.717731297881052	38.5245643593482	25.145031204053026
3	18.080840646972288	26.772997045371376	28.561974912041133	26.584187395615206
4	12.298494793924304	18.563643396564668	37.3981135225295	31.739748286981524
5	13.331404909394106	38.61268881501245	34.22694546657348	13.82896080901996
6	29.69733729091167	38.48803209412033	18.32029536064347	13.494335254324536
7	26.516309878302195	33.34601007610658	22.39467192800654	17.743008117584683
8	25.230139914771815	36.54732318025637	20.35016017287602	17.872376732095795
9	26.606903757702433	16.374196186139457	20.23894330948437	36.77995674667373
10-11	24.524307794479345	26.706706131787506	28.30039353576851	20.46859253796464
12-13	25.481416878922005	24.4404352961214	28.454986531063497	21.623161293893094
14-15	22.666880530812094	25.338578903857233	26.22731635033221	25.767224214998464
16-17	22.053667693711894	28.33684366326681	26.895179984067436	22.71430865895386
18-19	22.160028405105454	27.497199066119414	28.66002100459089	21.682751524184237
20-21	23.040001660813807	26.660763517842902	28.463704043408395	21.8355307779349
22-23	22.792105336394275	26.6123807078077	28.339254841832535	22.25625911396549
24-25	22.46416221993198	26.747088002045587	28.43744467344196	22.351305104580472
26-27	22.404939175416274	26.879745490337314	28.382969519629185	22.33234581461722
28-29	22.379684081678185	26.81759212876929	28.48157467644672	22.321149113105804
30-31	22.627952388769124	26.684430229045997	28.381329380049696	22.306288002135183
32-33	22.38024874182862	26.909557740600277	28.392681946432397	22.31751157113871
34-35	22.548909856414596	26.869282536347278	28.387994835955404	22.193812771282722
36-37	22.52262154285896	26.802595414914805	28.358960172166835	22.3158228700594
38-39	22.373530839900816	26.870311875059986	28.37852637459096	22.377630910448236
40-41	22.68051103580411	26.672801176965017	28.347680623597306	22.299007163633565
42-43	22.416547941453857	26.783437413880325	28.19255788832325	22.60745675634257
44-45	22.505811690895968	26.930859940606044	28.000154171496376	22.563174197001608
46-47	22.66437368815325	26.967285956597713	27.726651751169896	22.641688604079143
48-49	22.604146554864236	27.212922888115017	27.497849606986353	22.685080950034394
50-51	22.57958804948931	27.356097114957134	27.282605917997373	22.78170891755618
52-53	22.627286527129943	27.141661580636818	27.240404778934906	22.990647113298333
54-55	22.673003447696797	26.884754887060442	27.57495109835358	22.86729056688919
56-57	22.548318051089662	26.740242981355767	27.681895130165042	23.029543837389532
58-59	22.418653437444004	26.57640981978327	27.65142334865869	23.353513394114035
60-61	22.622769427899335	26.808598522921606	27.868791667693106	22.69984038148595
62-63	22.198641337559284	27.064595463759268	27.850119837133157	22.88664336154829
64-65	22.084401796726503	27.284410638163198	27.50510081749602	23.126086747614274
66-67	22.106258281010575	27.796568327766778	27.135177911680188	22.961995479542463
68-69	22.198601610635233	27.796718616850402	26.993733372831375	23.01094639968299
70-71	22.27124832460387	27.277651400264396	26.95749761723799	23.493602657893742
72-73	22.399867760631683	26.881576663609184	26.946548223970357	23.772007351788776
74-75	22.440014400866524	26.977494632095528	27.171026345804822	23.411464621233126
76-77	22.80227550378991	26.863466657695728	26.58269181769306	23.7515660208213
78-79	22.943123948831882	26.91264782517011	26.222496598402756	23.92173162759525
80-81	22.804287920697885	27.282710985601575	26.366179650566934	23.546821443133602
82-83	22.78661225974771	27.033206941757033	26.45240842084251	23.727772377652744
84-85	22.798925173124612	27.38937291175505	26.40841899140625	23.403282923714087
86-87	23.1931051353379	27.328856408985157	26.50507202345251	22.972966432224435
88-89	22.934154541772976	27.42650060864894	26.755813560752628	22.883531288825452
90-91	22.978340983407907	27.059870936798596	26.615712535616364	23.346075544177133
92-93	22.948852948990098	26.734303300845873	26.821394822290866	23.495448927873163
94-95	22.292251715917512	27.101953036037855	27.407504344249233	23.1982909037954
96-97	22.452926197969116	27.010196750956517	27.5458720852026	22.991004965871767
98-99	23.607055965477734	28.932732616087204	27.787597411043762	19.6726140073913
100	22.681340103546006	29.94978589296175	21.48976553089626	25.879108472595984
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11506.0
1	9886.5
2	5931.5
3	3462.0
4	3489.5
5	4927.0
6	7612.5
7	12607.5
8	20267.5
9	25301.0
10	25038.5
11	23836.0
12	26456.5
13	34214.5
14	53554.5
15	81195.5
16	103339.0
17	115307.5
18	116048.5
19	107109.5
20	92332.5
21	76520.5
22	66098.0
23	66815.5
24	78146.0
25	97628.0
26	124307.5
27	154550.0
28	184683.5
29	218279.5
30	254032.5
31	290005.0
32	330313.0
33	370002.5
34	408200.5
35	446080.0
36	487182.5
37	528348.0
38	563880.5
39	594982.5
40	623412.5
41	649425.5
42	672629.5
43	697709.5
44	722093.0
45	749817.0
46	771242.5
47	785304.5
48	798614.0
49	799022.5
50	794286.5
51	783363.0
52	760030.0
53	726485.5
54	686946.0
55	643020.0
56	598026.5
57	546049.0
58	486899.5
59	427856.5
60	361394.5
61	293723.5
62	232818.5
63	181218.0
64	136873.5
65	100536.0
66	74750.0
67	57415.0
68	43872.5
69	31847.0
70	23065.5
71	16843.5
72	11931.5
73	8366.0
74	5835.0
75	3915.5
76	2548.5
77	1536.0
78	961.0
79	654.0
80	455.0
81	314.0
82	219.5
83	150.0
84	101.0
85	70.5
86	50.0
87	44.5
88	34.5
89	23.0
90	14.0
91	7.0
92	5.0
93	2.0
94	1.0
95	2.5
96	4.5
97	5.5
98	7.0
99	11.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.008242457159600997
3	0.0
4	2.681345855432986E-5
5	0.0
6	0.010419709994212583
7	0.008891342856615782
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	3.75388419760618E-5
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.444830758341484E-5
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	1.7913985741547877E-4
50-51	0.014157612858427485
52-53	3.3082098289905794E-4
54-55	0.0
56-57	0.007925022764606254
58-59	0.023733290853198374
60-61	0.004679473241963492
62-63	0.0013371097692054708
64-65	0.0021246182889801783
66-67	0.0010338121830207462
68-69	0.0014997889926127727
70-71	3.0336256777726488E-5
72-73	0.005437218661820276
74-75	0.0018340389854936163
76-77	0.020118268010209866
78-79	1.3829847923534772E-4
80-81	3.238044046160794E-4
82-83	0.00390294714200522
84-85	0.0029891032774367783
86-87	0.003316049542434217
88-89	6.6096421989169184E-6
90-91	3.3437100917751454E-6
92-93	0.0
94-95	0.0018469026207204896
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	3804.0
20-21	9432.0
22-23	11828.0
24-25	11988.0
26-27	14084.0
28-29	21355.0
30-31	39793.0
32-33	47436.0
34-35	64861.0
36-37	98106.0
38-39	80323.0
40-41	128741.0
42-43	145106.0
44-45	176435.0
46-47	171228.0
48-49	152539.0
50-51	82731.0
52-53	25878.0
54-55	24262.0
56-57	24397.0
58-59	84005.0
60-61	144188.0
62-63	130847.0
64-65	135770.0
66-67	145360.0
68-69	150892.0
70-71	154850.0
72-73	139369.0
74-75	137427.0
76-77	144386.0
78-79	155458.0
80-81	160604.0
82-83	153323.0
84-85	148956.0
86-87	156438.0
88-89	171324.0
90-91	187102.0
92-93	194665.0
94-95	230689.0
96-97	674642.0
98-99	1268020.0
100-101	1.244471E7
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.30087245932887
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.80027752367742	69.7013801486317
2	8.827579661192843	14.177246969960242
3	2.37513923591576	5.7217725856925785
4	0.8917433900381343	2.8643108891960707
5	0.41123138237972173	1.6511119393873768
6	0.23101670072925534	1.1130505572740927
7	0.1288959846605577	0.7245322017322938
8	0.08388241422912304	0.5388664837274721
9	0.05359314380724846	0.38732185850042955
>10	0.18770296947320791	2.329329003772405
>50	0.005931427675489699	0.32649268841687623
>100	0.00291811136753866	0.4158079992557055
>500	7.339542301854299E-5	0.0347213234200875
>1k	1.465943073911083E-5	0.014055351032605983
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	5.362691710865972E-6	0.0
2	0.0	0.0	0.0	5.362691710865972E-6	0.0
3	0.0	0.0	0.0	1.0725383421731944E-5	0.0
4	0.0	3.75388419760618E-5	0.0	1.6088075132597917E-5	0.0
5	5.362691710865972E-6	3.75388419760618E-5	0.0	2.1450766843463888E-5	0.0
6	5.362691710865972E-6	3.75388419760618E-5	0.0	2.681345855432986E-5	5.362691710865972E-6
7	5.362691710865972E-6	3.75388419760618E-5	0.0	2.681345855432986E-5	5.362691710865972E-6
8	5.362691710865972E-6	4.826422539779374E-5	0.0	2.681345855432986E-5	5.362691710865972E-6
9	5.362691710865972E-6	1.0189114250645346E-4	0.0	8.044037566298958E-5	5.362691710865972E-6
10-11	1.6088075132597917E-5	1.0189114250645346E-4	0.0	1.0725383421731943E-4	5.362691710865972E-6
12-13	1.6088075132597917E-5	1.0189114250645346E-4	0.0	1.313859469162163E-4	5.362691710865972E-6
14-15	2.4132112698896875E-5	1.4747402204881422E-4	0.0	1.3942998448251525E-4	5.362691710865972E-6
16-17	4.290153368692777E-5	1.501553679042472E-4	0.0	1.5819940547054617E-4	1.0725383421731944E-5
18-19	4.826422539779374E-5	1.5819940547054617E-4	0.0	1.7160613474771107E-4	1.0725383421731944E-5
20-21	5.362691710865972E-5	1.7965017231401007E-4	0.0	2.118263225792059E-4	1.0725383421731944E-5
22-23	6.435230053039167E-5	1.9573824744660795E-4	0.0	2.6009054797699964E-4	2.681345855432986E-5
24-25	7.239633809669061E-5	2.440024728444017E-4	0.0	3.110361192302264E-4	4.826422539779374E-5
26-27	1.0725383421731943E-4	2.5740920212156666E-4	0.0	4.182899534475458E-4	6.167095467495869E-5
28-29	1.4479267619338121E-4	2.5740920212156666E-4	0.0	9.465150869678439E-4	6.435230053039167E-5
30-31	1.501553679042472E-4	2.7349727725416455E-4	0.0	0.003059415621049037	6.435230053039167E-5
32-33	1.5819940547054617E-4	2.7349727725416455E-4	0.0	0.007647198379694875	6.435230053039167E-5
34-35	1.6088075132597915E-4	3.110361192302264E-4	0.0	0.01346035619427359	6.971499224125763E-5
36-37	1.6356209718141213E-4	3.5125630706172114E-4	0.0	0.025255596612323294	7.239633809669061E-5
38-39	1.662434430368451E-4	3.539376529171541E-4	0.0	0.17147206745493945	7.77590298075566E-5
40-41	1.716061347477111E-4	4.182899534475458E-4	0.0	0.32299492174545746	8.044037566298958E-5
42-43	1.7428748060314408E-4	4.370593744355767E-4	0.0	0.34679454755828065	8.580306737385555E-5
44-45	1.7696882645857706E-4	4.799609081225045E-4	0.0	0.3775334964449644	9.38471049401545E-5
46-47	1.7696882645857706E-4	5.040930208214013E-4	0.0	0.4107902290898997	1.0725383421731943E-4
48-49	1.7696882645857706E-4	5.067743666768343E-4	0.0	0.4427572343783718	1.2334190934991734E-4
50-51	1.7696882645857706E-4	5.872147423398239E-4	0.0	0.4753704440180032	1.2334190934991734E-4
52-53	1.7696882645857706E-4	5.952587799061228E-4	0.0	0.9061796012645656	1.4479267619338124E-4
54-55	1.7696882645857706E-4	5.979401257615558E-4	0.0	1.4579952156209632	1.6088075132597915E-4
56-57	1.7696882645857706E-4	6.220722384604528E-4	0.0	2.054474007891308	1.689247888922781E-4
58-59	1.7696882645857706E-4	6.247535843158857E-4	0.0	2.6782837584660815	1.8233151816944305E-4
60-61	1.7696882645857706E-4	6.274349301713187E-4	0.0	2.8441759451958646	1.8233151816944305E-4
62-63	1.7696882645857706E-4	6.274349301713187E-4	0.0	2.8504958773771203	1.87694209880309E-4
64-65	1.7965017231401007E-4	6.327976218821847E-4	0.0	2.8553088931876225	1.87694209880309E-4
66-67	1.8233151816944305E-4	6.327976218821847E-4	0.0	2.8583817155379485	1.9305690159117497E-4
68-69	1.8233151816944305E-4	6.596110804365145E-4	5.362691710865972E-6	2.8610737867768035	1.9841959330204096E-4
70-71	1.87694209880309E-4	6.703364638582465E-4	5.362691710865972E-6	2.8632161821152944	2.091449767237729E-4
72-73	1.87694209880309E-4	6.756991555691125E-4	5.362691710865972E-6	2.865141388439495	2.3595843527810277E-4
74-75	1.87694209880309E-4	6.864245389908444E-4	5.362691710865972E-6	2.866664392885381	2.3595843527810277E-4
76-77	1.87694209880309E-4	7.132379975451743E-4	5.362691710865972E-6	2.867999703121387	2.3863978113353575E-4
78-79	1.87694209880309E-4	7.293260726777722E-4	5.362691710865972E-6	2.8688121509155833	2.4132112698896873E-4
80-81	1.87694209880309E-4	7.293260726777722E-4	5.362691710865972E-6	2.869080285501126	2.520465104107007E-4
82-83	1.87694209880309E-4	7.320074185332052E-4	5.362691710865972E-6	2.8693966843120675	2.681345855432986E-4
84-85	1.87694209880309E-4	7.346887643886382E-4	5.362691710865972E-6	2.869573653138526	2.681345855432986E-4
86-87	1.87694209880309E-4	7.480954936658031E-4	5.362691710865972E-6	2.869654093514189	2.681345855432986E-4
88	1.87694209880309E-4	8.044037566298958E-4	5.362691710865972E-6	2.8696621375517553	2.681345855432986E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	25465	0.0	52.680386	1
GTATCAA	46875	0.0	38.403625	1
ATCTCGT	16430	0.0	38.19259	94
TATCTCG	19385	0.0	32.10391	94
TCAACGC	58440	0.0	30.211514	4
ATCAACG	58360	0.0	30.179068	3
CAACGCA	59485	0.0	29.637217	5
TATCAAC	60135	0.0	29.584223	2
AACGCAG	61655	0.0	28.678007	6
TATGCCG	10170	0.0	26.452488	86-87
CTCGTAT	10110	0.0	26.015348	82-83
TCGTATG	10625	0.0	25.66808	84-85
TCTCGTA	10395	0.0	25.250973	82-83
ACGCAGA	71040	0.0	24.807783	7
CGCAGAG	71775	0.0	24.557444	8
CCGTCTT	11385	0.0	24.51958	90-91
CGTATGC	11070	0.0	24.442078	84-85
GCCGTCT	11585	0.0	24.290411	90-91
ATGCCGT	11425	0.0	24.235188	88-89
GTACATG	64675	0.0	24.014904	1
>>END_MODULE