FastQCFastQC Report
Fri 27 May 2016
SRR2140071_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2140071_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26653800
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA407850.052.3677641
GTATCAA757750.038.229351
TCAACGC931600.030.4703164
ATCAACG934800.030.3614863
CAACGCA951800.029.81925
TATCAAC966450.029.6062452
AACGCAG976900.029.092016
ACGCAGA1125250.025.1939477
CGCAGAG1146350.024.7240738
GTACATG1063700.023.2765451
TACATGG1067550.022.7963472
ACATGGG1082550.021.9470253
GCAGAGT1328100.021.2418829
GAGTACT719350.020.2320712-13
CAGAGTA1286500.019.00481210-11
CATGGGG699750.018.998564
GTACTTT777650.018.65155214-15
AGAGTAC1199000.018.62820210-11
GTGGTAT173900.017.4169671
AGTACTT773850.017.28523312-13
Produced by FastQC (version 0.11.5)