##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2140071_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 26653800 Sequences flagged as poor quality 0 Sequence length 19-100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.48624672654556 33.0 31.0 34.0 28.0 34.0 2 31.9911748793793 33.0 31.0 34.0 30.0 34.0 3 32.025105726012804 33.0 31.0 34.0 30.0 34.0 4 35.35089675768558 37.0 35.0 37.0 33.0 37.0 5 35.34638246704035 37.0 35.0 37.0 33.0 37.0 6 35.41666854257179 37.0 35.0 37.0 33.0 37.0 7 35.49884222887543 37.0 35.0 37.0 33.0 37.0 8 35.67516237834755 37.0 35.0 37.0 33.0 37.0 9 37.617819597956014 39.0 38.0 39.0 35.0 39.0 10-11 37.74682452408287 39.0 38.0 39.0 35.0 39.0 12-13 37.77248915726838 39.0 38.0 39.0 35.0 39.0 14-15 39.16780691683738 41.0 39.0 41.0 36.0 41.0 16-17 39.197223135162716 41.0 39.0 41.0 36.0 41.0 18-19 39.239353244190326 41.0 39.0 41.0 36.0 41.0 20-21 39.25800865839071 41.0 39.0 41.0 36.0 41.0 22-23 39.21716891738514 41.0 39.0 41.0 36.0 41.0 24-25 39.19775141114394 41.0 39.0 41.0 36.0 41.0 26-27 39.11780777731958 41.0 39.0 41.0 35.5 41.0 28-29 39.054269754995076 41.0 39.0 41.0 35.0 41.0 30-31 38.97173366943288 40.5 39.0 41.0 35.0 41.0 32-33 38.89121772320231 40.0 38.0 41.0 35.0 41.0 34-35 38.817722591856835 40.0 38.0 41.0 35.0 41.0 36-37 38.76394794547528 40.0 38.0 41.0 35.0 41.0 38-39 38.7200714668287 40.0 38.0 41.0 35.0 41.0 40-41 38.67614827962332 40.0 38.0 41.0 35.0 41.0 42-43 38.61020936099886 40.0 38.0 41.0 34.5 41.0 44-45 38.53354091177065 40.0 38.0 41.0 34.0 41.0 46-47 38.46008624686007 40.0 38.0 41.0 34.0 41.0 48-49 38.3815791273137 40.0 38.0 41.0 34.0 41.0 50-51 38.267531966130996 40.0 38.0 41.0 34.0 41.0 52-53 38.15967788784769 40.0 37.0 41.0 33.0 41.0 54-55 38.014345667897956 40.0 37.0 41.0 33.0 41.0 56-57 37.851740002092015 40.0 37.0 41.0 33.0 41.0 58-59 37.65420511270063 39.0 36.0 41.0 33.0 41.0 60-61 37.44950823071068 39.0 36.0 41.0 33.0 41.0 62-63 37.21667543467689 39.0 35.0 40.5 32.5 41.0 64-65 37.21316502376612 39.0 35.0 41.0 33.0 41.0 66-67 36.993789265160245 38.0 35.0 40.0 33.0 41.0 68-69 36.71045255266657 37.0 35.0 40.0 32.0 41.0 70-71 36.40692052459124 37.0 35.0 39.5 32.0 41.0 72-73 36.09005537160201 36.5 35.0 39.0 32.0 41.0 74-75 35.75838460970845 36.0 35.0 39.0 32.0 40.5 76-77 35.43056570893984 35.5 35.0 37.5 31.5 39.5 78-79 35.14066857239878 35.0 35.0 37.0 31.0 39.0 80-81 34.859055624073086 35.0 34.5 37.0 31.0 39.0 82-83 34.61387843921226 35.0 34.0 36.0 31.0 38.0 84-85 34.38785723504695 35.0 34.0 36.0 31.0 37.0 86-87 34.19475842715659 35.0 34.0 35.5 31.0 37.0 88-89 34.05478270085362 35.0 34.0 35.0 31.0 36.5 90-91 33.94343515955918 35.0 34.0 35.0 31.0 36.0 92-93 33.866017548498014 35.0 34.0 35.0 31.0 36.0 94-95 33.814830756661436 35.0 34.0 35.0 31.0 36.0 96-97 33.812581914166074 35.0 34.0 35.0 31.0 35.5 98-99 33.85495182106312 35.0 34.0 35.0 31.0 35.0 100 33.27452745780991 35.0 33.0 35.0 29.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 6.0 17 269.0 18 2738.0 19 9880.0 20 20254.0 21 31970.0 22 45891.0 23 62629.0 24 84107.0 25 112354.0 26 146198.0 27 186049.0 28 229983.0 29 282077.0 30 345334.0 31 428837.0 32 542380.0 33 710191.0 34 1015474.0 35 1540784.0 36 2623643.0 37 5488845.0 38 8033520.0 39 4471772.0 40 238615.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.55640846708537 18.83688629763861 9.919073452941044 27.687631782334975 2 14.619812387272713 21.908755573282164 38.483523196072596 24.987908843372526 3 18.13809287981451 27.05560933150245 28.469524045351886 26.33677374333116 4 12.252763593741694 18.6919639311346 37.369959867997764 31.685312607125944 5 13.211283194141174 38.881236446585476 34.05810053350742 13.849379825765931 6 29.45870440338652 38.58256164457835 18.359173861059062 13.599560090976068 7 26.463560578843342 33.6097263091936 22.27481269763249 17.651900414330566 8 25.227761144752343 36.549640201397175 20.27635834290045 17.946240310950035 9 26.660798835438097 16.518972904426384 20.246163774020964 36.57406448611455 10-11 24.452428921954844 26.940256173603768 28.154008058888415 20.45330684555298 12-13 25.510246193788504 24.681478813527526 28.382416390908617 21.425858601775357 14-15 22.68236986846153 25.526230781352005 26.30414800141068 25.487251348775786 16-17 21.92380286988122 28.545241190125857 26.96871281250626 22.562243127486653 18-19 22.01352340004052 27.634436740727402 28.813658840390488 21.53838101884159 20-21 22.985756359114074 26.72677067076126 28.667171934927733 21.62030103519694 22-23 22.69916079661934 26.685282499370356 28.49853766050203 22.117019043508275 24-25 22.337644396904157 26.835629933928274 28.620436295143858 22.206289374023708 26-27 22.249529418916815 26.964760992308328 28.59400584729068 22.191703741484172 28-29 22.22135121486141 26.919248094081144 28.688012466016282 22.171388225041166 30-31 22.45894738635768 26.822955376512898 28.57270090114496 22.145396335984465 32-33 22.226287302477672 26.976385223698625 28.61334501063535 22.183982463188357 34-35 22.39747384799618 26.974086359246847 28.604943360774804 22.023496431982164 36-37 22.365210663844508 26.880157045319493 28.568430382112027 22.186201908723973 38-39 22.236902133753667 27.00382403676258 28.625738786585025 22.133535042898732 40-41 22.4588240391678 26.85757921855259 28.497151211820377 22.186445530459228 42-43 22.35376682682929 26.94649386554516 28.310970748391213 22.38876855923434 44-45 22.4031903768798 27.127243606330232 28.095162858886958 22.374403157903014 46-47 22.564948331818478 27.164772772884678 27.718440496730494 22.55183839856635 48-49 22.529368532036813 27.27283102419486 27.58738199333104 22.610418450437283 50-51 22.490930878234796 27.443935791594036 27.489692923586073 22.57544040658509 52-53 22.650472199034066 27.313600952732976 27.430255026760182 22.605671821472775 54-55 22.593602739857253 27.251785103985178 27.478793021477333 22.67581913468024 56-57 22.553845030973356 27.33378331479891 27.461789223209244 22.650582431018492 58-59 22.545202423476724 27.357865557163112 27.4366158489728 22.660316170387368 60-61 22.65683395536336 27.352513801803013 27.423114881421096 22.56753736141253 62-63 22.555942433787106 27.46100094213831 27.430858629667643 22.552197994406946 64-65 22.536823338844762 27.388547739487016 27.327367543537857 22.747261378130364 66-67 22.59324719323825 27.480093908575228 27.200627756203744 22.72603114198278 68-69 22.536029663892336 27.450427718639574 27.264031937464722 22.749510680003365 70-71 22.62082214814694 27.20736123104307 27.3074822405134 22.864334380296587 72-73 22.599357387227393 27.22474748087033 27.261895813785507 22.91399931811677 74-75 22.520210263203026 27.412401800433063 27.259295259559007 22.808092676804907 76-77 22.710632474080846 27.29346878855275 27.145893595772435 22.850005141593968 78-79 22.642724090737655 27.32389500249098 27.133325822781902 22.900055083989464 80-81 22.680475462831787 27.406218803287334 27.094224489953632 22.819081243927247 82-83 22.731706671087764 27.342957828829718 27.16896137403644 22.756374126046087 84-85 22.730013051769497 27.374719151671634 27.13104040420641 22.76422739235246 86-87 22.594911183931053 27.425846203188186 27.256410192067072 22.722832420813692 88-89 22.670204991398 27.360715506905457 27.163391870374465 22.805687631322076 90-91 22.734610079952986 27.376974629530565 27.179379179959057 22.709036110557392 92-93 22.75933445156889 27.368858641124184 27.20352048405688 22.66828642325005 94-95 22.62313125557878 27.356471247058167 27.270086586620845 22.750310910742215 96-97 22.693036422544303 27.47872523098768 27.343440995881053 22.484797350586962 98-99 23.729801456875617 29.4551570968519 27.494574487148004 19.320466959124484 100 22.747976033303686 30.28032360022097 21.310595548079192 25.66110481839615 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 25661.0 1 21220.5 2 12074.5 3 7045.5 4 6957.0 5 8876.5 6 12612.5 7 20347.5 8 32660.5 9 40863.0 10 40123.0 11 37647.0 12 41186.5 13 52581.5 14 81061.0 15 121373.5 16 154247.5 17 173341.5 18 175866.0 19 162889.0 20 140508.0 21 117204.5 22 103145.0 23 105259.5 24 122809.5 25 153571.5 26 196143.0 27 244529.5 28 293373.0 29 345856.0 30 399538.0 31 452697.5 32 513326.5 33 573567.5 34 631231.5 35 687068.5 36 746775.0 37 804895.0 38 852324.5 39 892637.5 40 928803.5 41 960152.5 42 985717.5 43 1014558.5 44 1042050.5 45 1073566.5 46 1098390.5 47 1115837.5 48 1135679.5 49 1136253.0 50 1124343.5 51 1103133.0 52 1068294.5 53 1025613.5 54 976713.5 55 919292.0 56 856945.0 57 785624.5 58 705526.5 59 622405.0 60 529468.5 61 433235.5 62 344358.5 63 269685.0 64 205023.5 65 151244.5 66 110578.5 67 82572.0 68 62220.0 69 44856.5 70 32110.5 71 23432.0 72 16820.0 73 11790.5 74 8112.0 75 5350.5 76 3512.5 77 2283.5 78 1438.0 79 905.5 80 594.0 81 396.5 82 284.5 83 198.5 84 136.0 85 95.0 86 72.0 87 61.0 88 43.0 89 24.0 90 19.5 91 14.5 92 7.5 93 6.0 94 6.0 95 6.0 96 9.5 97 14.5 98 15.5 99 13.5 100 30.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.007841283419249789 3 0.0 4 3.376629223600388E-5 5 0.0 6 0.01063638205434122 7 0.008910549340056577 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.252534347822825E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 6.388584726491064E-5 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 1.3414864743324783E-4 50-51 0.014191366710452513 52-53 3.6451083419872417E-4 54-55 0.0 56-57 0.008058905180157982 58-59 0.024450451293902407 60-61 0.004778332502928859 62-63 0.001296951694407954 64-65 0.0021427594083192666 66-67 0.0010928422868973198 68-69 0.0015786025224934508 70-71 2.422743713646318E-5 72-73 0.005467401795687821 74-75 0.0019351782196993465 76-77 0.020562146981825118 78-79 8.468818599576473E-5 80-81 3.2855281278227776E-4 82-83 0.0040545353487282925 84-85 0.003137812240693041 86-87 0.003426984404998436 88-89 2.417174605695371E-6 90-91 2.4479725059395156E-6 92-93 0.0 94-95 0.0018609222272252517 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 5915.0 20-21 14948.0 22-23 18726.0 24-25 18931.0 26-27 22320.0 28-29 32283.0 30-31 60232.0 32-33 72330.0 34-35 96802.0 36-37 148429.0 38-39 186172.0 40-41 192115.0 42-43 222086.0 44-45 269979.0 46-47 260649.0 48-49 234898.0 50-51 199529.0 52-53 176965.0 54-55 185686.0 56-57 203348.0 58-59 212606.0 60-61 213585.0 62-63 197857.0 64-65 203727.0 66-67 216130.0 68-69 227061.0 70-71 228744.0 72-73 209104.0 74-75 204863.0 76-77 216046.0 78-79 231347.0 80-81 240946.0 82-83 228608.0 84-85 222293.0 86-87 231184.0 88-89 254043.0 90-91 274137.0 92-93 286201.0 94-95 334032.0 96-97 891736.0 98-99 1646032.0 100-101 1.7061175E7 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.67171276346413 #Duplication Level Percentage of deduplicated Percentage of total 1 87.35375446435837 70.46976988958892 2 8.467096990314728 13.661104326861226 3 2.15351542713016 5.211833339073991 4 0.867687230810087 2.7999126020974776 5 0.4208924304924708 1.6977056628502445 6 0.2317363430964912 1.1216740624281427 7 0.13634157980719822 0.7699236134746251 8 0.08572817243863493 0.5532670802165023 9 0.06548833023454417 0.4754750189436028 >10 0.21004268968828868 2.5813344557070934 >50 0.005454600398900949 0.300466686759307 >100 0.002178759566418958 0.2949678817504189 >500 5.1968595450876894E-5 0.02894901247299956 >1k 3.10130682862836E-5 0.033616367775443454 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.1255430745334624E-5 0.0 2 0.0 3.7518102484448745E-6 0.0 1.8759051242224373E-5 0.0 3 3.7518102484448745E-6 7.503620496889749E-6 0.0 1.8759051242224373E-5 0.0 4 3.7518102484448745E-6 3.7518102484448745E-5 0.0 3.0014481987558996E-5 0.0 5 7.503620496889749E-6 4.126991273289362E-5 0.0 3.376629223600387E-5 0.0 6 1.5007240993779498E-5 4.126991273289362E-5 0.0 3.376629223600387E-5 1.1255430745334624E-5 7 1.5007240993779498E-5 4.126991273289362E-5 0.0 3.376629223600387E-5 1.1255430745334624E-5 8 1.8759051242224373E-5 4.877353322978337E-5 0.0 3.376629223600387E-5 1.1255430745334624E-5 9 2.2510861490669247E-5 9.754706645956674E-5 0.0 3.7518102484448745E-5 1.1255430745334624E-5 10-11 2.813857686333656E-5 9.754706645956674E-5 0.0 5.627715372667312E-5 1.1255430745334624E-5 12-13 3.0014481987558996E-5 1.0129887670801161E-4 0.0 7.691211009311992E-5 1.5007240993779498E-5 14-15 3.7518102484448745E-5 1.7445917655268666E-4 0.0 9.004344596267699E-5 2.063495636644681E-5 16-17 6.378077422356287E-5 1.8759051242224373E-4 0.0 1.1255430745334624E-4 2.6262671739114122E-5 18-19 6.753258447200774E-5 2.1760499440980272E-4 0.0 1.3506516894401548E-4 3.376629223600387E-5 20-21 7.128439472045262E-5 2.5512309689425147E-4 0.0 1.707073663042418E-4 3.564219736022631E-5 22-23 7.128439472045262E-5 2.776339583849207E-4 0.0 2.063495636644681E-4 4.314581785711606E-5 24-25 8.816754083845456E-5 2.982689147513675E-4 0.0 2.945171045029227E-4 4.877353322978337E-5 26-27 9.379525621112186E-5 3.1515206086936946E-4 0.0 4.164509375773811E-4 4.877353322978337E-5 28-29 9.754706645956674E-5 3.207797762420368E-4 0.0 9.173176057447719E-4 4.877353322978337E-5 30-31 1.0880249720490136E-4 3.564219736022631E-4 0.0 0.0028982734169236656 4.877353322978337E-5 32-33 1.1255430745334624E-4 3.6017378385070796E-4 0.0 0.0075186277378835285 4.877353322978337E-5 34-35 1.2005792795023599E-4 4.1457503245315863E-4 0.0 0.013127584059308616 4.877353322978337E-5 36-37 1.256856433229033E-4 4.821076169251664E-4 0.0 0.022711583338961047 4.877353322978337E-5 38-39 1.2756154844712573E-4 5.046184784158357E-4 0.0 0.046878869054318706 5.440124860245068E-5 40-41 1.3506516894401548E-4 5.83406493633178E-4 0.0 0.0734398097081842 5.627715372667312E-5 42-43 1.3506516894401548E-4 6.246764063660717E-4 0.0 0.0902948172493228 5.627715372667312E-5 44-45 1.3506516894401548E-4 6.940848959623018E-4 0.0 0.11074030719822314 5.8153058850895555E-5 46-47 1.5007240993779498E-4 7.484861445647526E-4 0.0 0.131983056824918 7.128439472045262E-5 48-49 1.5007240993779498E-4 7.541138599374197E-4 0.0 0.15174946911884984 8.253982546578724E-5 50-51 1.5007240993779498E-4 8.610404520180987E-4 0.0 0.17191544920424107 8.253982546578724E-5 52-53 1.5007240993779498E-4 8.666681673907661E-4 0.0 0.2196009574619754 9.191935108689942E-5 54-55 1.5007240993779498E-4 8.741717878876558E-4 0.0 0.2747563199243635 1.0317478183223406E-4 56-57 1.5007240993779498E-4 8.966826493783251E-4 0.0 0.3280132664010385 1.0505068695645649E-4 58-59 1.5007240993779498E-4 9.023103647509923E-4 0.0 0.3553452040609594 1.0692659208067892E-4 60-61 1.5007240993779498E-4 9.173176057447718E-4 0.0 0.37481897515551255 1.0880249720490136E-4 62-63 1.5194831506201744E-4 9.304489416143289E-4 0.0 0.39047903113252147 1.1255430745334624E-4 64-65 1.5382422018623987E-4 9.754706645956674E-4 0.0 0.3981458553752185 1.1630611770179111E-4 66-67 1.5757603043468473E-4 9.886020004652245E-4 0.0 0.40429882418266816 1.2193383307445843E-4 68-69 1.5945193555890716E-4 0.0010298719131981182 0.0 0.41070128837163933 1.2380973819868087E-4 70-71 1.6132784068312962E-4 0.0010617623003098995 0.0 0.41595945043483484 1.3881697919246034E-4 72-73 1.6320374580735205E-4 0.001076769541303679 0.0 0.4199326174879379 1.5757603043468473E-4 74-75 1.6507965093157448E-4 0.0010974044976701258 0.0 0.4229903428404205 1.5945193555890716E-4 76-77 1.6507965093157448E-4 0.0011386744104030194 0.0 0.4257347920371579 1.6132784068312962E-4 78-79 1.6883146118001937E-4 0.0011555575565210213 0.0 0.4275956899203866 1.6132784068312962E-4 80-81 1.6883146118001937E-4 0.0011555575565210213 0.0 0.428180972319144 1.6695555605579694E-4 82-83 1.6883146118001937E-4 0.0011630611770179111 0.0 0.4287831378640194 1.7258327142846423E-4 84-85 1.707073663042418E-4 0.001170564797514801 0.0 0.42898761152255965 1.7258327142846423E-4 86-87 1.7445917655268666E-4 0.0011893238487570253 0.0 0.42907202725314963 1.7258327142846423E-4 88 1.7633508167690912E-4 0.0013056299664588164 0.0 0.4290757790633981 1.7258327142846423E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 40785 0.0 52.367764 1 GTATCAA 75775 0.0 38.22935 1 TCAACGC 93160 0.0 30.470316 4 ATCAACG 93480 0.0 30.361486 3 CAACGCA 95180 0.0 29.8192 5 TATCAAC 96645 0.0 29.606245 2 AACGCAG 97690 0.0 29.09201 6 ACGCAGA 112525 0.0 25.193947 7 CGCAGAG 114635 0.0 24.724073 8 GTACATG 106370 0.0 23.276545 1 TACATGG 106755 0.0 22.796347 2 ACATGGG 108255 0.0 21.947025 3 GCAGAGT 132810 0.0 21.241882 9 GAGTACT 71935 0.0 20.23207 12-13 CAGAGTA 128650 0.0 19.004812 10-11 CATGGGG 69975 0.0 18.99856 4 GTACTTT 77765 0.0 18.651552 14-15 AGAGTAC 119900 0.0 18.628202 10-11 GTGGTAT 17390 0.0 17.416967 1 AGTACTT 77385 0.0 17.285233 12-13 >>END_MODULE